최근 농업분야에서 정밀농업을 구현하기 위한 다양한 노력들을 기울이고 있다. 정밀농업분야에서 가장 대표적인 연구분야는 영상정보를 이용하여 작물의 생육 및 수량을 예측하는 것이다. 특히, 여러 영상정보 중에서 다중분광 혹은 초분광영상정보를 기반으로 한 작물의 생육 및 수량예측이 가장 활발하게 연구되고 있는 분야이다. 각각의 센싱장비를 통해서 획득된 영상정보는 여러 방법으로 활용할 수 있으며, 가장 보편적으로 활용하고 있는 방법이 다중분광 및 초분광정보를 이용하여 다양한 식생지수로 산출하고 이를 기반으로 작물의 생육을 예측하는 것이다. 식생지수는 식물체내의 광합성색소함량 및 광합성효율과 높은 상관관계를 보이는 파장을 기반으로 산출된 지수이다. 현재까지 약 150종의 식생지수가 알려져 있으나 농업분야에서 주로 이용하고 있는 식생수는 일부에 불과하다. 이런 이유에서 본 논문에서는 다양한 식생지수들 중 주요한 식생지수들을 선별하여 식생지수들의 산출공식, 적용방법과 관련된 내용을 소개하였다.

백색, 적색(680±20nm), 황색(600 ± 20 nm), 녹색(525 ± 25 nm),청색(400 ± 10 nm), 흑색(365 ± 10 nm) 및 변환(다양한 파장)램프를 가지는 솔라트랩을 잡곡 및 두류포장에서 설치하여 나비목, 노린재목 및 딱정벌레목의 주요 해충에 대한 유인효과를 잡곡 및 두류포장에 설치하여 10일 간격으로 조사하여 평가하였다. 채집된 충수는 조사시기와 램프의 색깔에 따라 크게 영향을 받았으며, 조사시기와 램프의 색깔의 상호작용 효과도 있었다. 암청색램프에서 매미충류를 제외하고 가장 많은해충이 유인되었다. 따라서 암청색램프는 해충의 대량포획뿐만아니라 예찰을 위해 효과적으로 사용될 수 있다. 솔라트랩의 암청색램프와 페로몬의 조합은 잡곡 및 두류 해충의 포획효과를 증진시킬 수 있어 종합관리를 위한 유망한 수단으로사용될 수 있을 것으로 여겨진다.

Riptortus pedestris (F.) (Hemiptera: Alydidae) attacks several leguminous crops and its reproductive success is found to be affected by the leguminous host types. Ooencyrtus nezarae Ishii (Hymenoptera: Encyrtidae) is an egg parasitoid of R. pedestris and its quality attributes depend on the host egg quality. We, thus, investigated the performance of O. nezarae on eggs of R. pedestris fed on different leguminous seeds. Eggs collected from R. pedestris female adults obtained from rearing on one of the four hosts such as soybean, adzuki bean, mung bean, and cowpea seeds were exposed as a batch (n=15) to a single 4 to 5 day-old mated female O. nezarae. The eggs exposed were collected after 24 h. Number of parasitized eggs, sex ratio, development times, and adult emergence were recorded. The maximum number of eggs parasitized by O. nezarae were the eggs of mung bean fed R. pedestris. Eggs produced from the adzuki bean fed bugs had the lowest parasitization. There was no difference recorded in the developmental time of parasitoids emerged from the eggs of R. pedestris fed on different legumes. Proportion of male O. nezarae was highest on the eggs of cowpea seed fed adult bugs (26%); the lowest of that was found on the eggs of mung bean fed bugs (22.4%). Ooencyrtus nezarae can successfully parasitize eggs of R. pedestris regardless of hosts food sources. However, eggs of mung bean fed R. pedestris, among the tested legumes, were found to be of the best quality for reproductive success of O. nezarae.

썩덩나무노린재는 두과작물에서 과실에 이르기까지 다양한 작물을 가해하는 대표적인 다식성 노린재의 일종이다. 과실이 썩덩나무노린재의 발육에 필요한 영양원인지 불필요한 영양원인지 아직도 분명하지 않다. 그리하여 사과와 오렌지의 단독먹이 및 콩-땅콩과의 먹이조합에 따른 썩덩 나무노린재의 발육, 사망률 및 증식률을 평가하였다. 썩덩나무노린재 약충은 사과단독먹이에서만 높은 사망률로 성충으로 발육하지 못하였다. 물 만 제공한 것은 3령으로 발육하지 못하였고, 사과단독먹이에서 4령으로 발육하지 못하였다. 하지만 오렌지단독먹이에서 14%가 성충으로 우화하 였으나, 약충의 발육기간이 약 74일로 가장 길었다. 콩-땅콩과 조합한 먹이에서 노린재의 사망률은 38~44%를 나타내었다. 총 산란수는 169~190 개 였으며, 부화률은 81~83%이었다. 따라서 사과는 썩덩나무노린재의 발육에 불완전한 먹이 이었으나, 오렌지는 발육이 지연되기는 하였으나 이루지는 것으로 평가되었다.

The brown marmorated stink bug Halyomorpha halys Stål (Hemiptera: Pentatomidae), is native to Korea, Japan, and China. H. halys is one of the major polyphagous pentatomids with a wide host range encompassing ornamental shrubs, trees, and cultivated crops such as millet, sesame, soybean, apple, yuzu, pear, cherry, and peach and inflicting losses of several crops in Korea. However, study on dietary importance of these hosts on the development rate of H. halys is sparse. We evaluated fruits of apple and orange with or without soybean plus peanuts as food sources to investigate development rate, mortality and fecundity of the stink bug. Not apple but orange only diet could support development up to adult stage but with higher mortality. First instars were found to molt into second instars without feeding. The longest time H. halys took to develop was in its fifth instar. Overall shortest developmental period (39.82) days was recorded on those fed on orange+soybean and peanut whereas those fed on orange only diet had longest development period (78.92 days). Generational mortality of those fed on diets consisting of soybean and peanuts ranged from 43 to 53%. Those only fed on water could not develop into third instars. H. halys could not develop into fourth instar in apple only diet. However, on orange only diet 20% H. halys could emerge as adults. Fecundity was measured for the first day of oviposition per female. Fecundity was recorded highest (28 eggs) on orange+soybean and peanut diets. The results suggest that H. halys require leguminous seeds in order to develop fully and lay fertile eggs.

Alfalfa weevil, Hypera postica Gyllenhal, is regarded as one of the most important insect pests on Chinese milk vetch and hairy vetch. The insect usually feed on leaves, stems and pods of the green manure which causes severe reduction of biomass. This study was conducted to observe the occurrence of H. postica larva and adult on Chinese milk vetch in 2009 to 2010, and to evaluate host plants preference of 11 different seedlings in 2010, Milyang, Korea. Occurrence of H. postica larvae on Chinese milk vetch was observed to increase from early April clearly and its peak population was showed during middle to late April. The 1st generation of newly emerged adult of H. postica was appeared on 20th May. Newly emerged H. postica adult was fed on green manure such as Chinese milk vetch and hairy vetch as well as various seedlings located at nearby upland. And then the insects were migrated into aestivating places in middle to late of June. Thus, host plant preference of H. postica adults were evaluated with 11 different seedlings using transparent acrylcage and at Chinese milk vetch field. As a result, H. postica adult preferred on Chinese cabbage, followed by soybean, kale, foxtail millet and proso millet. The damaged rating of seedlings were shown to have the same trend with adult occurrence. In particular, few H. postica adults were occurred on eggplant, lettuce, corn, crown daisy and peanut, and thereby those crops were regarded as non-preference plants due to the absence of damage. These informations can be used by upland farmers who sowing from late May to early June for effective management of H. postica.

Bakanae disease is one of the most serious and oldest problems of rice production, which was first described in 1828 in Japan (Ito and Kimura 1931). This disease may infect rice plants from the pre-emergence stage to the mature stage, with severe infection of rice seeds resulting poor germination or withering (Iqbal et al. 2011). Under favorable environmental conditions, infected plants have the capacity to produce numerous conidia that subsequently infect proximate healthy plants, resulting in major yield loss (Ou 1985). One hundred sixty nine NILs, YR28297 (BC6F4) generated by five backcrosses of Shingwang with the genetic background of susceptible japonica variety, Ilpum were used for QTL analysis. Rice bakanae disease pathogen, CF283, was mainly used in this study and inoculation and evaluation of bakanae disease was performed with the method of the large-scale screening method developed by Kim et al. (2014). A major QTL for resistance against bakanae disease on chromosome 1 was identified using SSR marker, RM9, which explaining 65 % of the total phenotype variation. The major QTL designated as qBK1 and mapped to a 4.4 Mbp region between RM24 (19.30 Mb) and RM11295 (23.72 Mb). The results of this study are expected to provide useful information toward developing resistant rice lines to this detrimental fungal disease.

Soybean mosaic virus (SMV) is a prevalent pathogen that causes significant yield reduction in soybean production worldwide. SMV belongs to potyvirus and causes typical symptoms such as mild mosaic, mosaic and lethal necrosis. SMV is seed-borne and also transmitted by aphid. Eleven SMV strains, G1 to G7, G5H, G6H, G7H, and G7a were reported in soybean varieties in Korea. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) method allowed one-step detection of gene amplification by simple procedure and needed only a simple incubator for isothermal template. This RT-LAMP method allowed direct detection of RNA from virus-infected plants without thermal cycling and gel electrophoresis. In this study, we designed RT-LAMP primers named SML-F3/B3/FIP/BIP from coat protein gene sequence of SMV. After the reaction of RT-LAMP, products were identified by electrophoresis and with the detective fluorescent dye, SYBR Green I. under daylight and UV light. Opmtimal reaction condition was at 58℃ for 60min and the primers of RT-LAMP showed the specificity for nine SMV strains tested in this study.

Generally, the virus was detected by the ELISA using the serological method and RT-PCR based on the genetic information. Recently, NGS (next-generation nucleotide sequencing) has been used in genome analysis and diseases diagnostics. To identify distribution aspects of viruses, we collected diseased samples twice in soybean breeding field. After extraction of total RNA from the collected bulk samples, RNA was sequenced by the NGS method. The NGS data were analyzed using the bioinformatics software. With newly produced NGS data, the identification of distribution aspects of organisms in field was estimated in this study. Sequence based identification method should be more accurate diagnostic tools of the target diseases and be able to predict occurrence of potential and new pathogens. NGS method will also provide the basic data by identifying the distribution of using bacteria. In this study, we analyzed the extracted RNA from the collection of approximately 3000 samples. Consequently, we confirmed the following types: the 7,089 kinds of bacteria including Burkholderiaceae, the 13,397 kinds of Eukaryota, the 952 kinds of viruses from the first bulk samples, the 4,160 kinds of bacteria including Burkholderiaceae, the 10,475 kinds of Eukaryota, and the 576 kinds of viruses from the second bulk samples

Bakanae disease incidence threat is an increasing trend in the top rice growing countries. Despite it is essential to identify the resistant genes and underlying mechanisms of bakanae disease to develop resistant varieties, there are very limited genetic studies on bakanae disease in rice. The indica rice variety Shingwang was selected as resistant donor to bakanae disease. One hundred sixty nine NILs, YR28297 (BC6F4) generated by five backcrosses of Shingwang with the genetic background of susceptible japonica variety, Ilpum were used for QTL analysis. Rice bakanae disease pathogen, CF283, was mainly used in this study and inoculation and evaluation of bakanae disease was performed with the method of the large-scale screening method developed by Kim et al. (2014). The proportion of healthy plants of Shingwang and Ilpum after inoculation was confirmed using bakanae disease pathogen, CF283. While inoculated Ilpum showed thin and yellowish-green phenotype which is typical symptom of Bakanae disease, Shingwang showed similar healthy phenotype with control plants. A major QTL for resistance against bakanae disease on chromosome 1 was identified using SSR marker, RM9, which explaining 65 % of the total phenotype variation. The major QTL designated as qBK1 and mapped to a 4.4 Mbp region between RM24 (19.30 Mb) and RM11295 (23.72 Mb). The information of qBK1 could be useful for improving rice bakanae disease resistance in marker-assisted breeding.

국내 콩에서 발생하는 세균병해인 불마름병, 들불병, 세균점무늬병, 세균갈색점무늬병의 다중 진단을 위한 PCR 방법을 요약하면 다음과 같다.1. 콩에 발생하는 각각의 세균들은 서로 다른 박테리오신(bacteriocin) 이나 파이토톡신(phytotoxin)을 생산하는데 이와 관련한 유전자를 목적으로 하여 진단프라이머를 설계하였다.2. 불마름병은 glycinecin A, 들불병은 tabtoxin, 세균점무늬병은 coronatine과 세균갈색점무늬병은 syringopeptin을 목적유전자로 하여 다중 진단프라이머 조합을 설계하였다.3. 1차 선발로 각각의 균주에 대한 단일 진단 프라이머를선발하였으며, 여기선 선발된 21개의 프라이머들을 조합하여 4종 다중진단프라이머 선발을 위한 2차 선발에 이용하였다. 최종적으로 280 bp의 불마름병, 355bp의 세균갈색점무늬병, 563 bp의 들불병과 815 bp의세균점무늬병으로 구성된 다중진단 프라이머 조합이개발되었다.4. 선발된 4종 다중 진단 프라이머 조합의 경우 다른 세균들과의 비특이적 반응이 있는지 확인하기 위한 3차선발을 거쳐 그 특이성을 검증하였다.

It has been reported that bacterial diseases of domestic soybean are bacterial pustule, wildfire, bacterial blight and bacterial brown spot. Among these bacterial diseases, bacterial blight infected by Gram-negative Xanthomonas campestris pv. glycines had been the most issue in soybean diseases. In recently, issue in soybean disease was shifted to wildfire by Gram-negative Pseudomonas syringae pv. tabaci. When infected in nature, symptom of wildfire generally surrounded infected-area of bacterial blight and formed pustule in infective center. Because optimum growth temperature of Wildfire is lower than these of bacterial pustule, Wildfire caused damage to crop yield in later growth stage. In this study, we checked symptoms of bacterial diseases with growth stages of soybean. Also, we surveyed disease severity of single infection, simultaneous infection and sequential infection of bacterial pustule and wildfire.