Nine fish and one clam species were collected from freshwater environments in Korea, including four lakes, two streams, and the Nakdong River, to investigate the host-associated bacteria. Hundreds of bacterial strains were isolated from the samples using a cell sorter and a dilution plating method. After identification of the bacterial strains using 16S rRNA gene sequences, 42 strains with greater than 98.7% sequence similarity with validly published species were determined to be unrecorded bacterial species in Korea. These strains were phylogenetically diverse and assigned to four phyla, six classes, 17 orders, 27 families, and 32 genera. At the genus level, the unrecorded species were classified as Corynebacterium, Mycobacterium, Mycolicibacterium, Gordonia, Williamsia, Modestobacter, Brachybacterium, Sanquibacter, Arthrobacter, and Mycolicibacterium of the class Actinobacteria; Empedobacter, and Flavobacterium of the class Flavobacteriia; Fictibacillus, Psychrobacillus, Cohnella, Paenibacillus, Rummeliibacillus, Enterococcus, and Vagococcus of the class Bacilli; Aquamicrobium, Paracoccus, and Sphingomonas of the class Alphaproteobacteria; Achromobacter, Delftia, and Deefgea of the class Betaproteobacteria; and Aeromonas, Providencia, Yersinia, Marinomonas, Acinetobacter, and Pseudomonas of the class Gammaproteobacteria. The 42 unrecorded species were subjected to further taxonomic characterization using gram staining, cellular and colony morphological determination, biochemical analyses, and phylogenetic analyses. This paper provides detailed descriptions of the 42 previously unrecorded bacterial species.

본 연구는 여름철 장미의 수출 환경 조건에서 황금(Scutellaria baicalensis) 추출물(SC용액, 특허출원번호 10-2019-0109867)의 절화수명연장 효과를 확인하고 수입산 절화보존제의 대체 가능성을 알아보고자 수행되었다. 건식 및 습식 수송 조건으로 구분하고, 습식 수송 조건은 절화보존제 처리 유무와 종류 에 대한 비교분석을 위해 물(tap water), Floralife 1%, SC용 액 300mg･L-1를 처리하였다. 그 결과, 절화수명 연장에는 습식수송 조건에서 절화보존제를 사용하는 것이 유리하였다. 특히 황금추출물 SC용액 처리구의 절화수명은 수돗물(7.7일)보다 1.7일 연장되었으며, 이러한 효과는 수입산 절화보존제인 Floralife의 효과와 유사하였다. 절화수명 종료 증상은 건식 수송 조건 경우 화색의 퇴화 증상이 상대적으로 많았으며, 습식 수송 조건의 경우 일반적인 장미 절화의 노화 증상인 꽃목굽 음과 꽃잎마름 증상이 전체의 60~70%를 차지하였다. 결론적으로 여름철 장미의 수출 환경에서 SC용액은 절화수명을 효 과적으로 연장시킬 수 있었으며, 그 효과는 기존 절화보존제인 floralife와 유사하여 친환경 대체 절화 보존제로 적용이 가능할 것으로 판단된다.

In this study, the effects of heat-moisture treatment (boiling or steaming for 45 min) prior to hot air drying (70 o C for 8 h) on water activity (Aw), moisture (MC), Brix, color, and texture of dried sweet potato slices were investigated to identify the best pretreatment condition for producing dried sweet potato with the best eating quality. Dried sweet potato slices pretreated by boiling (BL) showed significantly lower Aw, MC, and hardness while Brix was significantly higher than with steaming (ST) treatment. There were no significant differences for the color, springiness, cohesiveness, gumminess, and brittleness indexes. At 8 h drying, the Aw, MC, Brix, and hardness of the BL and ST samples were 0.81 and 0.82, 19.71% and 25.53%, 53.80% and 49.40%, and 20.49 kg/cm 2 and 31.98 kg/ cm 2 , respectively. This information will be useful for manufacturers in the production of dried sweet potato slices. These findings provide evidence of the feasibility of heat-moisture treatments in the production of dried sweet potato.

The purpose of this study was to analyze the current status and contents of color-related education programs as lifelong education and to determine the necessity of color education and directions for future development. The findings of this study, first show that the general welfare center, which is striving to develop abilities and promote welfare, was opened mainly for professional education, rather than hobbyoriented education. Accordingly, pricing was set at a lower level than the three institutions and centers, but without a variety of programs or hobby-oriented courses. Second, as the purpose of the set-up is to be established, the general welfare center should be considered an area for hobbies along with expertise, and various courses should be opened. Although the department store’s cultural center focused on programs for cultural and leisure use and promotion, it is necessary to extend the program to attempt to approach it in a short-term process rather than a one-time class. The lifelong education center established as a subsidiary of the university’ was opened as an area for of painting such as oil painting and watercolor and drawing which have much in common school should learn and start basic theory and practice, so there was much difference. Third, if it is changed to a developed form of color-oriented education according to the purpose of each center and institution presented in the results of this research, a good response will be possible to further improve the quality of life and develop professionalism and creativity.

Hepatic stellate cells (HSCs) play essential roles in normal and pathophysiological function in liver. In steady state, HSCs contribute to retinoid storage, immune tolerance, and extracellular matrix (ECM) homeostasis. Upon liver injury, they become activated and lead to morphological and functional changes. Studies have demonstrated that activation of HSCs by various stimuli such as toxins, microbial infection, or metabolic overload can promote the fibrotic changes in liver by production of ECM. Herein, we provide current knowledge about the basic characteristics of HSCs and the mechanism by which they are activated.

Osteoarthritis is a disorder characterized by a loss of cartilage as common aging-associated disease in humans and animals. However, unlike human clinical trials, investigational studies in pet animals are constrained by a lack of interest and funds. In addition, pet owners would often prefer the lowest cost method to treat arthritis of pet animals. Here, we report the outstanding and inexpensive way to prepare chondrocytes for cartilage repair using rabbit adipose derived mesenchymal stem cells (MSCs). This study focused on the development and enhancement of pre-chondrogenic condensation under external electric fields even without additional growth factors. We found that highly compact structures were formed within 3 days in micromass cultures of rabbit MSCs under electrical stimulation (ES), showing increased COL2A1 gene expression compared with their control 3D micromass cultures and 2D monolayer cultures. We further found that ES enhanced the production of proteoglycan, a highly produced extracellular matrix component in chondrocytes. Collectively, these results provide the commercial potential of electrical stimulation driving chondrogenesis of mesenchymal stem cells for repair of cartilage, which is a budget-friendly regimen.