묘목 생산성과 적절한 품질을 유지하기 위하여 시비는 매우 중요하지만, 수종에 따른 최적의 시비량을 결정하기는 어렵다. 이 연구에서는 경제림 육성 수종인 물푸레나무, 들메나무, 잣나무, 전나무를 대상으로 복합비료 1x(N 6.9 g m-2, P 3.1 g m-2, K 3.7 g m-2), 2x(1x의 2배), 4x(1x의 4배) 처리가 묘목의 생장과 양분변화에 미치는 영향을 양분벡터분석(Vector diagnosis)을 이용하여 정량화하였다. 시비량이 증가함에 따라 물푸레나무와 들메나무의 수고와 근원경은 증가하는 경향을 보였고 잣나무와 전나무는 시비량에 따른 차이를 보이지 않았다. 처리 1x, 2x, 4x는 대조에 비하여 물푸레나무의 건중량을 각각 43, 11, 87% 증가시켰고, 들메나무는 각각 36, 145, 143% 더 증가시켰다. 반면 잣나무와 전나무 건중량은 시비량에 따른 유의한 차이가 없었다. 시비에 대한 양분벡터반응은 수종과 시비량에 따라 상이한 경향을 보였는데, 물푸레나무 조직의 질소 농도는 생장 및 질소함량의 증가만큼 증가하지 않는 “양분결핍” 상태였고, 들메나무는 흡수한 것보다 생장이 증가하여 농도가 감소하는 “양분희석” 상태였으며, 잣나무와 전나무는 생장이 거의 증가하지 않으면서 농도는 약간 감소하는 “과량길항” 현상을 보였다. 본 연구는 물푸레나무와 들메나무는 처리 2x 또는 그 이상의 시비가 요구되고, 잣나무와 전나무의 경우 초기 시비를 하지 않아도 생장과 양분 흡수에 문제가 없음을 보여주고 있다.

수변완충림으로 조성된 3년생 포플러 4클론에 대하여 biomass 생산능력과 주요 비점오염원 중 하 나인 질소의 저장능력을 조사하였다. 현사시 72-31 및 미루나무 교잡종 Dorskamp 클론의 지상부 biomass 구성 비율은 줄기, 가지, 잎의 순으로 높았으며, 잎과 가지의 비율은 두 클론간에 차이를 보 였다. 지상부 biomass의 질소 함량은 잎, 가지, 줄기의 순으로 높았고 72-31 클론의 잎과 가지의 질 소함량은 Dorskamp에 비해 높았으나 줄기의 질소 함량은 낮게 나타났다. 3년생 Ay48 클론의 지상 부 biomass 추정량은 37.5 ton․ha -1로 가장 높았으며, 72-31 클론이 가장 낮았다. 3년생 포플러 클론의 지상부 biomass의 질소 저장능력은 biomass 생산량의 순위와 일치하였다. 지상부 biomass 생산능력 이 가장 우수한 3년생 Ay48 클론은 218.3 kg․ha -1의 질소를 지상부 biomass에 저장할 수 있는 것으로 추정되었다.

Background: Panax ginseng C. A. Meyer is wood-cultivated ginseng (WCG) in Korea which depends on an artificial forest growth method. To produce this type of ginseng, various P. ginseng cultivars can be used. To obtain a WCG similar to wild ginseng (WG), this method is usually performed in a mountain using seeds or seedlings of cultivated ginseng (CG) and WG. Recently, the WCG industry is suffering a problem in that Panax notoginseng (Burk.) F. H. Chen or Panax quinquefolium L. are being sold as WCG Korean market; These morphological similarities have created confusion among customers. Methods and Results: WCG samples were collected from five areas in Korea. After polymerase chain reaction (PCR) amplification using the primer pair labeled with fluorescence dye (FAM, NED, PET, or VIC), fragment analysis were performed. PCR products were separated by capillary electrophoresis with an ABI 3730 DNA analyzer. From the results, WCG cultivated in Korea showed very diverse genetic background. Conclusions: In this study, we tried to develop a method to discriminate between WCG, P. notoginseng or P. quinquefolium using simple sequence repeat (SSR) markers. Furthermore, we analyzed the genetic diversity of WCG collected from five cultivation areas in Korea.

Background : Panax ginseng C.A. Meyer is a perennial herb belongs to the family Araliaceae. Wild-cultivated ginseng (WCG) is a specific type of ginseng in Korea which cultivated on artificial forest cultivation method. To obtain a WCG which is similar to wild ginseng (WG), this method usually performed in a mountain using seeds or seedlings of cultivated ginseng (CG) and WG. WCG is very expensive because it is difficult to cultivate. However, systematic cultivation method have not yet been developed compared to high added value. Furthermore, very high price of WCG caused the problem that Panax notoginseng or Panax quinquefolium are sold as WCG in Korean market. In this study, we analyzed the genetic diversity of WCG collected from five areas in Korea using SSR markers. Methods and Results : WCG samples were collected from five areas in Korea (Bucheon, Cheongju, Hoengseong, Judeok and Ulsan). DNA extraction was performed using CTAB method. SSR markers were collected from the published papers. After test PCR using the markers, one of the primer pair was labeled with fluorescence dye (FAM, NED, PET, or VIC) and GeneScan analysis were performed. DNA amplification was conducted using T-100 Thermal Cycler (Bio-Rad). PCR products were separated by capillary electrophoresis on the ABI 3730 DNA analyzer (Applied Biosystems). Conclusion : Eight SSR markers were collected from the published literature and used for the analysis. From the 8 tested SSR markers, 7 SSR markers showed polymorphism between varieties. GenScan analysis were performed using the selected SSR markers to analyze the phylogenetic relationship of WCG. From the results, WCG cultivated in Korea showed that they have a very diverse genetic background.

Background : Wild-cultivated ginseng (WCG) prices are very different according to root ages. Generally, two methods are used to discriminate the root ages of Panax ginseng C.A. Meyer. The first method is the yearly determination method by the ring dyeing method, and the second method is the confirmation the number of stem vestiges in the rhizome. In this study, we analyzed the agronomic and growth characteristics of the WCG cultivated in Korea. In this study, to determine the appropriate root ages discrimination method for the determination of the root ages of WCG, the root ages of WCG and cultivated ginseng was examined. Methods and Results : We examined the cultivated ginseng (CG) and WCG that was collected and sold by regional groups at the Korean market. WCG does not form annual rings, which are clear and regular in wild ginseng. Therefore, it is impossible to identify the age of WCG by using the annual growth rings staining method. However, the age can be estimated by determining the number of stem vestiges in the rhizome. Conclusion : From the results of the Study on identification of root age for quality evealuation in WCG in Korea. Appropriate root ages discrimination method of WCG was confirmation the number of stem vestiges in the rhizome.

Background : Panax ginseng C.A. Meyer is a representative medicinal plants and it has been used in traditional medicine because the plant have many effective component such as saponins. To obtain a wild-cultivated ginseng (WCG) which is similar to wild ginseng (WG), this method usually performed in a mountain using seeds or seedlings of cultivated ginseng (CG) and WG. WCG is very expensive because it is difficult to cultivate. However, systematic cultivation method have not been developed compared to their high added value. Furthermore, very high price of WCG caused the problem that Panax notoginseng or Panax quinquefolium are sold as WCG in Korean market. This is concerned as a serious problem to consumers. In this study, we analyzed the agronomic and growth characteristics of the WCG cultivated in Korea. Methods and Results : We examined the WCG that was collected and sold by regional groups at the Korean market. The root age, growth conditions, and quality level of the cultivated WCG were confirmed. WCG samples were collected from five areas in Korea (Bucheon, Cheongju, Hoengseong, Judeok and Ulsan). The main root diameter, root shape index, rhizome length, and root weight showed high level of variation and they did not form annual rings. Conclusion : Agronomic and growth characteristics of WCG showed high variations according to cultivating regions.