Italian ryegrass (Lolium multiflorum L.) is an annual forage crop widely cultivated across the globe for its excellent nutritional value and high productivity. Despite its importance, studies on differentially expressed genes (DEGs) involved in its morphogenesis remain limited. In this study, we employed a high-yielding mutant line developed through radiation mutagenesis to identify candidate genes associated with morphogenesis, focusing on Expansins (EXPs), GRFs (Growth regulating factors), GIFs (GRF-interacting factors), and growth hormone-related genes. RT-qPCR primers were designed, and differential gene expression analysis was performed. Gene expression was assessed in the leaves of seedlings at one to three weeks of age, comparing the control cultivar 'Kowinearly' with the mutant line. Expression patterns fell into four distinct categories: (1) genes consistently exhibiting lower expression in the mutant line across all developmental stages; (2) genes showing persistently low expression in the mutant, while the control displayed a sharp increase at early stages followed by a decline; (3) genes with low expression in the control but a marked early-stage increase in the mutant; and (4) genes with decreasing expression over time in the mutant, contrasting with gradually increasing expression in the control. These expression profiles highlight Actin and GRF6 as Group 1 genes, AXR1 (Auxin-resistant protein 1) and EXPB6 (c) as Group 2 genes, PEVMPP1 (Pyrophosphate-energized vacuolar membrane proton pump 1) as a Group 3 gene, and EF1A (Elongation factor 1-alpha) as a Group 4 gene, all of which are key candidate genes for the development of high-yielding Italian ryegrass cultivars.

This study was conducted in Cheonan of central region in South Korea to evaluate the forage characteristics of early-maturing Italian ryegrass (IRG) within September, 2021 to June, 2023. The three different varieties of Italian ryegrass were prepared in this study 'Kogreen', 'Kospeed' and 'Kowinearly'. The results of heading date in this study, 'Kogreen' was the earliest in among cultivars and recorded 24th of April, whereas 'Kowinearly' was recorded the latest. In case of plant length parameter, 'Kowinearly' was the highest, in addition lodge tolerance score 'Kowinearly' also superior in whole cultivars. The dry matter yield of 'Kowinearly' was recorded significantly the highest (11,027 kg/ha), in contrast 'Kospeed' was recorded significantly the lowest (8,455 kg/ha). As a result of ADF and NDF content 'Kospeed' was recorded excellent value as compared the other two cultivars. The CP content of this study was excellent in the order of 'Kospeed' > 'Kowinearly' > 'Kogreen'. In case of the monosaccharides content 'Kogreen' had relatively higher glucose and fructose content than other four cultivars, therefore the 'Kogreen' has advantage for preparing high quality of silage. In conclusion, among the three different early varieties of Italian ryegrass, 'Kowinearly' was the superior in forage productivity, 'Kospeed' was the highest in feed value and 'Kospeed' had the highest in glucose and fructose content.

The aim of this study was to investigate the feasibility of discrimination 12 different cultivar of sorghum × sudangrass hybrid (Sorghum genus) seed through near infrared spectroscopy (NIRS). The amount of samples for develop to the best discriminant equation was 360. Whole samples were applied different three spectra range (visible, NIR and full range) within 680-2500 nm wavelength and the spectrastar 2500 Near near infrared was used to measure spectra. The calibration equation for discriminant analysis was developed partial least square (PLS) regression and discrimination equation (DE) analysis. The PLS discriminant analysis model for three spectra range developed with mathematic pretreatment 1,8,8,1 successfully discriminated 12 different sorghum genus. External validation indicated that all samples were discriminated correctly. The whole discriminant accuracy shown 82 ~ 100 % in NIR full range spectra. The results demonstrated the usefulness of NIRS combined with chemometrics as a rapid method for discrimination of sorghum × sudangrass hybrid cultivar through seed.

본 시험은 사료가치가 우수하고 엔도파이트에 감염되지 않은 톨 페스큐 신품종을 개발하기 위하여 2010년부터 2017년까지 농촌진흥청 국립축산과학원 초지사료과에서 수행되었다. 톨 페스큐 신품종 ‘그린마스터4호’는 6배체 품종으로서 잎은 녹색이며, 출수기가 5월 16일경으로서 대비품종인 ‘Fawn’ 보다 5일 늦은 중생품종이고, 출수기에 초장은 74.6cm 정도이다. 지역적응시험 4개 지역평균 건물수량은 ‘그린마스터4호’가 16.2톤/ha로서 대비품종인 ‘Fawn’보다 5% 많았으며, 지역별 생산성이 4개 지역 모두에서 ‘Fawn’보다 우수하였다. ‘그린마스터4호’의 사료가치는 조단백질(CP) 함량이 15.2%, 건물소화율(IVDMD)이 70.0%, 가소화양분총량(TDN)이 63.6%, 중성세제불용섬유(NDF)가 58.7%, 산성세제불용섬유(ADF)는 32.0%로서 Fawn보다 높거나 대등하였다. ‘그린마스터4호’는 엔도파이트에 감염되지 않는 품종이면서 ‘Fawn’보다 국내 기후환경 적응성과 영속성이 우수하여 초지조성에 알맞은 품종으로서 우리나라 전국에서 재배 가능하다.