This study was carried out to investigate the chemical change of rutin and fatty acids of buckwheat (Fagopyrum esculentum) during germination. The content of rutin in buckwheat before germination was 31.5㎎% and that after 7 days was increased to l, 660.3㎎%. The content of stearic acid was increased slightly after 3 day during germination but those of other fatty acids were decreased gradually.

The blocked N-terminus and N-terminal sequence ol soybean β-amylase were determined by analyzing the acidic peptides derived on peptic digestion of the enzyme. The acidic peptides were separated from the digest on a Dowex 50×2 column(1×5㎝) and purified by reversed phase-high performance liquid chromatography(RP-HPLC). The major acidic peptide, PEP-1, was a heptapeptide. The N-terminal 7 amino acid sequence of soybean β-amylase was deduced to be acetyl-Ala-Thr-Ser-Asp-Ser-Asn-Met- from the results of sequence analysis of PEP-1 and amino acid analysis of other acidic peptides.