‘Seismic Performance Evaluation Method for Existing Buildings (2013)’ developed in accordance with the overseas guidelines ASCE 41 - 06 is the most widely used procedure among domestic seismic performance evaluation guidelines in Korea. However, unlike ASCE 41 - 06, it stipulates that the final performance should be derived as the gravity load distribution ratio of the lateral force resistance system in the guideline. Therefore, in the case of a dual steel structure system with slender braces, where the internal moment frame is mostly responsible for the gravity load, the evaluation of slender braces based on gravity load distribution ratio is difficult to be achieved. In this research, we propose an objective evaluation process for such system by evaluating seismic performance for large-scale factory facilities as an example.

A strain of Saccharomyces cerevisiae BY-366 was isolated to produce a strong sucrose hydrolyzing enzyme. After entrapment of yeast cell invertase with alginate, enzymatic properties of immobilized cells were investigated. The results are as follows. 1. The optimum pH of invertase in immobilized cells and non immobilized cells was 6.0 and 5.0, and pH stability of invertase in immobilized cells and non immobilized cells was 6.0 and 5.0, respectively. 2. Activation energy of immobilized cells was 4.7㎉/㏖. 3. The immobilized preparation exhibited high resistance to heat and urea induced denaturation. 4. The bead size less than 2㎜ in diameter was desirable. 5. In spite of repeated use, the enzyme activity of immobilized cells was inhibited slightly in batch reaction, and a small column of the immobilized preparation could hydrolyze relatively high concentration of sucrose almost quantitatively to more than 6 days.