The present study was carried out to investigate the effects of feeding dietary legislation sulfur on the lipid levels of chicken meat and growth performance in broilers. A total of 180 male broilers (Ross strain 308) were randomly assigned into three treated groups with triplicate of 20 birds each, T1 (control group), T2 (antibiotics 8 ppm), and T3 (0.3% sulfur premix containing 30% of sulfur) groups. The body weight gains over a 3-wk period were significantly higher in T2 and T3 groups than the T1 group, but not different between T2 and T3. The dressing percentage, weight of thigh meat, breast muscle and bursa of F were significantly high in T2 and T3 groups compared to the T1 group, but not different between T2 and T3. The weight of abdominal fat was significantly higher in T3 group than in T1 and T2 groups. The cholesterol content of the whole chicken meat was significantly lower in the T3 group as compared to the T1 and T2 groups. The oleic acid (18:1n-9) content of whole chicken meat from the T3 group was higher than that of the other groups. The results of this study suggests that feeding dietary sulfur as an antibiotic replacement may increase the oleic acid and reduce the cholesterol in chicken meat while improving the carcass characteristics and growth performance in broiler chickens

This study was conducted to evaluate the effects of dietary Korean red pine bark extract as an antibiotic replacements on cholesterol, fatty acids and the shelf-life of chicken meat. To accomplish this, chickens were fed the optimal level of red pine bark extract that was found to replace antibiotics in the diet of broilers. A total of 180 male broilers(Ross strain 308) were divided into three treated groups, T1(control group), T2(8 ppm of avilamycin) and T3(65 ppm of red pine bark extract per kg diet). The lipid content was reduced by 24.67% and 20.49% in T3 group, while the cholesterol level also decreased significantly in the T3 group by 20.49% and 20.55% when compared to the T1 and T2 groups, respectively. In addition, the saturated fatty acid level was lower in the T3 group than in the T1 and T2 groups, while the unsaturated fatty acid level of the T3 group was significantly higher than those of the other groups. The TBARS value of chicken thigh muscle containing its skin on the 7th day of low temperature storage was significantly lower by 23.86% and 21.17% in the T3 group than in the T1 and T2 groups, respectively. Evaluation of the color of the meat revealed that the L*value (lightness) and b*value(yellowness) were higher in the T3 group than in the T1 and the T2 groups, but that the pH was significantly lower in the T3. Based on the results of this study, the addition of 65 ppm red pine bark extract to the diet of broilers should improve their meat quality with respect to the lipid contents and shelf-life when compared to the addition of antibiotics.

Essential fatty acids (EFA) are fatty acids that must be obtained from the diet because they can not be biosynthesized by human or animals. Gamma fatty acids contain gamma-linolenic acid (GLA, 18:3n-6) and dihomo-gamma-linolenic acid (DHGLA, 20:3n-6) as intermediate metabolites of linoleic acid (LA, 18:2n-6), which is an EFA found in vegetable oils. GLA is an important essential fatty acid that is required by human and animals to function normally. Recently, studies have indicated that GLA may be an essential component of the cell membrane, as well as an active component of dietary supplements and medicine. GLA must beadministered through the diet because it is converted into DHGLA in the body quickly and completely. DHGLA is a key material involved in the metabolism of LA. GLA is biosysthesized by the rate limiting step of δc6-desaturase, which is an enzyme that desaturates LA, there by allowing it to be converted into DHGLA via chain elongation. In addition, DHGLA exerts bioactive effects via action as a precursor of eicosanoid series 1. Breast milk contains an abundant amount of GLA; however, GLA is also available directly in evening primrose oil, black currant seed oil, borage oil and hemp seed oil. In addition, GLA enriched animal and plant can be produced using biotechnology, and highly pure GLA can be extracted using supercritical fluids, such as supercritical carbon dioxide, which will allow economically feasible production of GLA for use in medicines.

Hemp seed oil and evening primrose oil were incorporated into the diets of laying hens for 5 weeks and the level of gamma fatty acid in the eggs that the treated hens laid was then evaluated. Hens were fed corn-soybean based diets that contained 5% tallow, 5% corn oil (CO), 5% hemp seed oil (HSO), or 5% evening primrose oil (EPO). The hemp seed oil and evening primrose oil influenced the amount of gamma linolenic acid found in the eggs through blood. The level of gamma linolenic acid in the plasma was significantly higher in hens that received the HSO and EPO diets than in those that received the tallow and CO diets. The HSO and EPO diets led to a 1.09% and 4.87% increase in egg gamma linolenic acids, respectively, when compared with eggs produced by hens treated with tallow and CO. Taken together, these data demonstrate that healthy eggs with increased gamma linolenic acids can be generated by minor diet modifications when hemp seed oil or evening primrose oil is included in the hen diet.

This study report that the gamma linolenic acid content of pork is higher in finishing pigs fed diets containing hemp seed oil, evening primrose oil or borage oil as the sources of gamma linolenic acid. Thirty six three crossing swines (Landrace×Yorkshire×Duroc), 80 kg of body weight, were randomly separated into four treatment groups with three pens per treatment and three animals per pen. The finishing swines were fed the experimental diets for 35 days until they reached the market weight of 110 kg. The animals were assigned to the four experimental diets: control diet containing 5.00% tallow, T1 containing 5.00% hemp seed oil(hemp seed oil 40 : soybean oil 60), T2 containing 5.00% evening primrose oil(primrose oil 40 : soybean oil 60), T3 containing 5.00% borage oil(borage oil 40 : soybean oil 60). The plasma triacylglycerol and total cholesterol content of the swine in the gamma fatty acids-fed groups were significantly(p<0.05) lower than those in the control group. No gamma linolenic acid was detected in the plasma of the control group, while the level of gamma linolenic acid treatment groups was significantly(p<0.05) higher than the control in the order of T3, T2 and T1. Moreover, the level of gamma linolenic acid increased with increasing number of feeding days. There was a significant difference between the treatment groups (p<0.05). There was a difference in the amount of saturated fatty acid and polyunsaturated fatty acid accumulated in the pork according to the treatment groups or the parts of the pork meat. The level of n-3 fatty acid of pork was highest in T1, which had been fed the hemp seed oil, followed in order by T3 and T2(p<0.05). The content of gamma linolenic acid in pork was highest in T3, which had been fed the borage oil, followed in order by T2 and T1(p<0.05). In particular, the level of gamma linolenic acid in pork increased in the order of the back fat, pork belly, ham and loin.

This study demonstrates that pork cholesterol levels are reduced in finishing swine fed β-cyclodextrin (βCD). The study subjects were 120 swine fed their respective chow diets containing 0, 5, 7, or 10% βCD for 35 consecutive days. Plasma total lipids, triglyceride and total cholesterol of the βCD- treated group were significantly lower than those of the control group (p<0.05). The levels of plasma lipid were significantly decreased by 63.22 mg, 73.98 mg, and 82.12 mg in the finishing swine group fed βCD at 5%, 7%, and 10% , respectively, compared to those in the control group (p<0.05). When 5, 7, and 10% βCD was administered to finishing swine, the triacylglyceride levels were decreased by 56.24 mg, 55.48 mg, and 60.02 mg, and total cholesterol concentration was reduced by 25.05 mg, 27.17 mg, and 30.19 mg, respectively, compared to those in the control group (p<0.05). Excretion of total steroid significantly (p<0.05) increased with increase of βCD supplementation. The cholesterol levels of swine back fat, belly, loin, and ham were significantly decreased with increase of βCD supplementation (p<0.05). The pork cholesterol was significantly (p<0.05) reduced by 15.31% in the βCD-treated group, compared to that of the control group. These results suggest that feeding βCD to finishing swine may produce novel functional pork with low cholesterol levels.

This study examined the effects of different levels of evening primrose oil(EPO) on the accumulation of γ-fatty acids in broiler meat. Six hundred one-day-old male chicks (Ross strain) from commercial broilers were divided randomly into 6 groups × 4 repeat pens. The broilers were fed experimental diets containing 4.0% tallow(control), 0.5% EPO, 0.7% mixed oil(EPO 70 : soy bean oil 30), 1.5% EPO, 3.0% EPO or 4.0% EPO for 35 days. There was a significant difference in body weight gain between the control and treatment groups except for the 0.5% EPO group (p<0.05). There was a significant difference in the percentage of thigh and breast weight against the carcass weight between control and treatment groups except for the 0.5% EPO group in the thigh and 0.5% EPO and 4.0% EPO groups in the breast weight (p<0.05). The saturated fatty acid levels of the skin and breast muscle lipid of the broilers fed diets containing EPO were significantly lower than that of the control group (p<0.05), while the level of unsaturated fatty acid was significantly higher than that of the control group (p<0.05). The γ-fatty acid(GLA, gamma-linolenic acid, 18:3n-6) level was particularly higher in the chicken meat lipids from the broilers fed EPO than in the control group (p< 0.05). This shows that feeding EPO to chicks can produce novel functional broiler meat that is enriched in gamma-linolenic acid.

The objective of this study was to determine the effect of dietary oils on the levels of the γ-linolenic acid in chicken meat lipids. Three hundred ten five, 1-d old, male, Ross strain, broiler chicks were fed for 35 d to compare diets containing evening primrose oil(EPO) and hemp seed oil(HO) to a control diet. Fatty acid composition of lipid from chicken skin, thigh and breast muscle were determined at the end of the trial. The level of γ-linolenic acid of lipids from chicken meat fed diets containing EPO or HO was significantly higher than that of the control group(p<0.05). The level of γ-linolenic acid of lipids from chicken skin was highest in the group, which had been fed the EPO 0.85%, followed in order by EPO 0.7%, 0.5%, EPO mixed oil, HO and HO mixed oil. There was a significant difference in the level of γ-linolenic acid of chicken skin between the control and treatment groups(p<0.05). The level of γ-linolenic acid of lipids from chicken thigh muscle was also similar to skin, and significantly higher than that of the control group(p<0.05). The level of γ-linolenic acid of lipids from chicken breast muscle was highest in the group, which had been fed the EPO 0.5%, followed in order by EPO 0.7%, 0.85%, HO 0.5% and HO mixed oil. There was a significant difference in the level of γ-linolenic acid of chicken breast muscle between the control and treatment groups(p<0.05).

The objective of this study was to determine the effect of dietary oils on the levels of the γ-linolenic acid in chicken meat lipids. Three hundred ten five, 1-d old, male, Ross strain, broiler chicks were fed for 35 d to compare diets containing evening primrose oil(EPO) and hemp seed oil(HO) to a control diet. Fatty acid composition of lipid from chicken skin, thigh and breast muscle were determined at the end of the trial. The level of γ-linolenic acid of lipids from chicken meat fed diets containing EPO or HO was significantly higher than that of the control group(p〈0.05). The level of γ-linolenic acid of lipids from chicken skin was highest in the group, which had been fed the EPO 0.85%, followed in order by EPO 0.7%, 0.5%, EPO mixed oil, HO and HO mixed oil. There was a significant difference in the level of γ-linolenic acid of chicken skin between the control and treatment groups(p〈0.05). The level of γ-linolenic acid of lipids from chicken thigh muscle was also similar to skin, and significantly higher than that of the control group(p〈0.05). The level of γ-linolenic acid of lipids from chicken breast muscle was highest in the group, which had been fed the EPO 0.5%, followed in order by EPO 0.7%, 0.85%, HO 0.5% and HO mixed oil. There was a significant difference in the level of γ-linolenic acid of chicken breast muscle between the control and treatment groups(p〈0.05).

We investigated the effect of different kinds of dietary added fat on the fatty acid composition, cholesterol content and quality of hens‘ eggs. The Haugh units, breaking strength, shell thickness and yolk color were not significant difference among the groups. Dietary fat notably altered the polyunsaturated fatty acid composition and cholesterol content in the egg yolk. The n-6 fatty acid of egg yolk was highest in the corn oil fed group with 31.61%, and this tended to decrease in the order of the tallow, linseed oil and fish oil fed groups(p<0.05). The n-3 fatty acid of egg yolk tended to increase in the linseed oil and fish oil fed groups as 9.74% and 5.16%, respectively(p<0.05). Theses alterations in the yolk fatty acid composition resulted in a reduced cholesterol content of the eggs. The ranges of cholesterol content showed values of 15.98-18.37 mg/g of yolk or 227-261 mg/60g of egg, respectively(p<0.05). The cholesterol content of egg yolk was highest in the tallow fed group, and this tended to be reduced in the order of the fish oil, linseed oil and corn oil fed groups(p<0.05). The reduction of the egg yolk cholesterol content in the other groups compared to the tallow group was significantly different, i.e., 13.01% in the fish oil group, 11.49% in the linseed oil group and 6.91% in the corn oil group, respectively(p<0.05). This result suggests that it is possible to reduce the cholesterol content or to increase the n-6 and n-3 polyunsaturated fatty acids in eggs by manipulation of the dietary added fats.

Response surface analysis was used to study dietary ratios of n-3/n-6 fatty acid and P/S to minimize plasma triglycerides, total cholesterol and LDL · VLDL-C levels and maximize plasma HDL · C levels of rats. Because the dietary components were not statistically independent, they were studied in combinations of two variables. The two-variable combinations were the most useful in locating the desired maximum or minimum plasma triglycerides, total cholesterol and LDL · VLDL-C response in terms of the proportions of the dietary components. Response surface contours and three dimensional plots were developed for each plasma lipid response. The contours and three dimensional plots were used to help determine those combinations of the dietary fatty acid ratios that would produce the desired minimum or maximum lpid responses. The statistical analyses indicated that the minimized plasma cholesterol response levels could be attained with a diet consisting of 2.26 n-3/n-6 fatty acid and 2.15 P/S ratios.

최근, 유한요소해석견과의 신뢰도를 향상시키기 위하여 활발하게 연구되고 있는 적응유한요소해석은 반복계산을 통해서 해석결과의 오차가 사용자에 의해 지정된 허용오차와 같아지도록 하는 해석방법이다. 이와 간은 적응유한요소해석은 해석결과의 오차평가와 이에 따른 유한요소의 재구성과정으로 나누어진다. rp방법에서는 절점의 위치를 이동시켜 요소의 크기를 조절하는 r방법과 형상함수찻수를 증가시키는 p방법을 동시에 적용함으로써 적응해석의 유효성을 향상시키고자 하였다. 제안한 rp방법의 특성을 규명하고 적응해석의 유효성을 보이기 위하여 전형적인 이차원 평면문제들을 해석하고 그 결과를 검토하였다.

The purpose of this study was to determine the effect of dietary γ-linolenic acid on plasma lipid metabolism and anti thrombotic activity in male Sprague Dwaley Strain rats. Rats weighing an average of 100~120g were fed a experimental diets containing 5% lard (saturated fatty acids), corn oil(linoleic acid), evening promise oil(EPO, 9% γ-linolenic acid) or borage oil(BO, 24% γ-linolenic acid) for 3Odays, respectively. Though there were no significant difference in the food intake among the groups, the body weight gain of the BO group was significantly lower than that of other group. The spleen weight of the lard group was significantly lower than that of other group. The bleeding time of the BO group was significantly longer than that of other group. The blood clotting time was significantly tended to long in EPO and BO groups compared with lard group. The plasma triacylglyceride and total cholesterol concentration were high in order of lard, com oil, EPO and BO, groups and there were significant differences among the groups. The plasma HDL-C concentrations were high in order of BO, EPO, com oil and lard groups and there were significant differences among the groups. The plasma LDL-C concentrations were significantly the highest in lard group, but the lowest in BO group. These data indicate that γ-linolenic acid has a antithrombotic activity, and decrease the plasma triacylglyceride, total cholesterol and LDL-C concentrations in rats.