This experimental study was designed to investigate the effects of Chrysanthemi Flos extract (CFE) on the change of cerebral hemodynamics〔regional cerebral blood flow (rCBF) and mean arterial blood pressure(MABP)〕in normal and cerebral ischemic rats, and to determine the mechanism of action of CFE. This study was also designed to investigate whether CFE inhibit lactate dehydrogenase (LDH) activity in neuronal cells and cytokines production in serum of cerebral ischemic rats. The results were as follows ; 1. CFE increased rCBF and increased MABP significantly in a dose-dependent manner. 2. The CFE-induced increase in rCBF was significnatly inhibited by pretreatment with indometh acin(IDN), an inhibitor of cyclooxygenase and methylene blue(MTB), an inhibitor of guanylate cyclase. 3. The CFE-induced increase in MABP was significnatly inhibited by pretreatment with IDN and MTB. 4. CFE 100㎍/㎖ significantly inhibited lactate dehydrogenase(LDH) activity in neuronal cells. 5. rCBF was significantly and stably increased by CFE (1㎎/㎏, i.p.) during the period of cerebral reperfusion, which contrasted with the findings of rapid and marked increase in control group. 6. In serum, by drawing from femoral arterial blood after middle cerebral arterial occlusion (MCAO) 1 hr and reperfusion 1 hr, sample group was significantly decreased IL-1β production compared to that of the control group. 7. In serum, by drawing from femoral arterial blood after MCAO 1 hr and reperfusion 1 hr, sample group was significantly decreased TNF-α production compared to that of the control group. 8. In serum, by drawing from femoral arterial blood after reperfusion 1 hr, sample group significantly increased TGF-β production compared to that of the control group. 9. In serum, by drawing from femoral arterial blood after reperfusion 1 hr, sample group increased IL-10 production compared to that of the control group. These results suggested that the mechanism of CFE was mediated by cyclooxygenase and guanylate cyclase, and CFE had inhibitive effect on the brain damage by inhibited LDH activity, IL-1β and TNF-α production, but accelerated TGF-β production and IL-10 production.thought that CFE should have an anti-ischemic effect on the improvement of cerebral hemodynamics and inhibitive effect on the brain damage.

The purpose of this study is to determine the effect of Semen Cuscutae on phenylephrine(PE) and KCl induced contraction of isolated rat thoracic aorta. Contractile force was measured with force displacement transducer under 1.5 g loading tension. Contraction evoked by PE (ED50) was relaxed significantly by Semen Cuscutae. The mean percent relaxing of PE induced contraction was 60.6% (p<0.01) after 300 ㎕/㎖ Semen Cuscutae, but the mean percent contraction of KCl induced contraction was 10.4% (p<0.05) after 300 ㎕/㎖ Semen Cuscutae. Vasorelaxation by Semen Cuscutae was significantly reduced after with and without endothelial cells. Pretreatment of aortic rings with L-NAME (100 μM), a nitric oxide synthase inhibitor, indomethacin (3 μM), an inhibitor of cyclooxygenase, methylene blue (10 μM), guanylyl cyclase inhibitor, propranolol (1 μM), a β-adrenoceptor antagonist, atropine (1 μM), a muscarinic receptor antagonist and diltiazem (10 μM), a Ca2+-channel blocker was recovered the effect aorta. These results indicate that Semen Cuscutae can relax EP induced contraction of isolated rat thoracic aorta and that this increasing contraction related to epithelium and various effect

우리나라 전통수산식품의 식품학적 연구의 일환으로서 담치젓국의 유리아미노산, 핵산관련물질, betaine, 총 creatinine 및 유기산 등의 정미성분과 지방산조성을 분석하였다. 담치젓국의 수분함량은 59.6%였고 조단백질 및 당은 9.4%였다. 각각 18.2%, VBN함량은 89.4mg/100g이었고 아미노질소 함량은 157.3mg/100g이었다. 총유리아미노산 함량은 건물량기준으로 10,520.5mg/100g 이었고 이중 glycine, arginine, aspartic acid 및 glutamic acid가 전체의 55.7%를 차지하였다. 핵산관련물질은 hypoxanthine의 함량이 건물량기준으로 8.77 μmole/g으로써 가장 많았다. 담치젓국의 엑스분질소중에서 유리아미노산질소, 암모니아질소, 핵산관련물질질소, TMAO질소, TMA질소, betaine질소 및 총 creatinine질소가 전체의 71.7%를 차지하고 있었고 이중 유리아미노산질소가 53.3%를 차지하여 상당히 많았다. 불휘발성유기산은 7종이 동정되었으며 succinic acid가 건물량기준으로 125.5mg/100g으로서 가장 많았고 다음으로 lactic acid(91.9mg/100g) 및 α-keto-glutaric acid(23.2mg/100g)의 순이었으며 oxalic acid, fumalic acid, malic acid 및 citric acid는 미량 검출되었다. 지방산조성은 폴리엔산이 45.5%, 포화산이 33.0%, 모노엔산이 21.5%였고 주요구성지방산은 16 : 0, 18 : 2, 22 : 6, 18 : 1 및 20 : 5였다. 관능검사 결과 유리아미노산이 맛에 기여하는 정도가 가장 컸고 다음으로 유기산 및 핵산관련물질의 순이었다. 담치젓국의 맛에는 유리아미노산과 불휘발성유기산이 중요한 역할을 하며 핵산관련물질과 TMAO, betaine 및 creatinine이 보조적 역할을 할 것으로 볼 수 있었다.

The crude lipid contents of dried sea food products varied remarkably from 1.2 to 29.9% for dried fish products, from 2.8 to 12.3％ for dried mollusk ones, and from 0.1 to 2.3％ for dried seaweed ones. In fatty acid composition of dried fish products, the saturated acid was the most predominant component for dried anchovies, hair tail, Kstsuobushi, the monoenoic acid was the most predominant one for dried yellow corvenia, flat head, common carp, sea eel and conger eel. And the polyenoic acid was the most abundant one for dried cod. Allaska pollack, flounder, sole fish, ray, smelt and sardine. The major fatty acids of these dried fish products were 16:0, 16:1, 18:0, 18:1, 20:5 and 22:6. Fatty acid composition of dried mollusk products were mainly consisted of polyenoic acid, and followed by saturated acid, monoenoic aced. The major fatty acids of these products were similiar to those of dried fish products. And in case of dried seaweed products, saturated acid such as 14:0, 16:0 was the most predominant component, while polyenoic acid was abundant one in dried laver and sea mustard. The main fatty acids of dried seaweed products were 14:0, 16:0, 18:1, 20:1, 20:4, and 20:5. Judging from the results, dried sea food products were abundant of the highly unsaturated fatty acids in spite of the drying processing and storage.