본 실험에서는 불포화 지방산인 oleic acid 함량이 시판 미강유의 자동산화에 미치는 영향에 대하여 연구하고저 시판 미강유에 oleic acid를 각각 0.1%, 0.3% 및 0.5%의 비율로 첨가하여 시료구를 만들었다. 이 시료구들은 45±0.3℃에서 40일간 저장하였으며, peroxide value, anisidine value 및 acid value의 변화에 근거를 두어 자동산화의 진행 정도를 규칙적으로 측정하였다. 자동산화가 진행되는 동안 0.1%, 0.3% 및 0.5%의 oleic acid 시료구의 peroxide value, acid value와 anisidine value의 경우 oleic acid를 첨가한 농토별 영향은 0.1%<0.3%<0.5%의 순서로 증가되었으며 저장기간이 길어질수록 자동산화 속도는 더욱 커짐를 알 수 있었다. 대조구의 유도기간은 19.8일인데 비하여 0.1%, 0.3% 및 0.5% oleic acid 시료구의 유도기간은 각각 18.3일, 16.8일, 15.5일이었다. 이상의 결과에서 보면, 45℃의 자동산화에서는 시판 미강유에 oleic acid를 0.1∼0.5% 농도로 첨가했을 때 oleic acid는 약한 산화촉진제로 작용했으며 농도가 높을수록 산화촉진 작용이 강했다.

Background : This study of investigate the quality characteristics of Korean Dioscoreae Rhizoma by species and various processing methods. We used two types of species D. Rhizoma, which were the long type shape of D. batatas and short type shape of D. japoninca, respectively. Methods and Results : The reaction of D. Rhizoma with acetic acid and iodine showed light blue to blue colours, respectively. When compared with loss of drying between two species, the long type species was about 2% more higher than that of short type of species. When compared with processing methods, the both steamed and dried samples was more higher 2.0 - 3.0% than that of non-steamed samples. The contents of acid-insoluble ash samples with peel was relatively higher than that of samples containing no-peel. The results of TLC analysis also showed the same Rf and colours when compared with the standard of D. Rhizoma. However, both dried sample with peel (DWP) showed more clear spot than those of dried sample except peel (DEP) and both steamed and dried sample except peel (SDEP). Conclusion : We sincerely hope that this study will be contributed to the standardization and quality control of korean herbal medicines.

Background: Leonurine is a the aerial part of Leonurus japonicus Houttuyn, which has been used as a traditional medicines and is registered in the Korean Pharmacopoeia.Methods and Results:In the present study we performed a heavy metals and thin layer chromatography and measured loss on drying, as well as the contents of total ash, acid-insoluble ash, ethanol soluble compounds, and leonurine, using 15 domestically collected L. japonicus samples. The methods were performed according to the ‘crude drugs test of the general test, processes and apparatus’, published by MFDS, Korea (2014). The purity test (heavy metals) indicated that levels of Pb and Hg were 0.35 - 3.64, and 0.001 ppm, respectively, whereas the levels of As and Cd were undetectable, and stachydrine was identified by thin layer chromatography (Rf : 0.15). We found that 5.93 - 10.62% (average: 8.58 ± 1.8%) of the sample mass was lost during drying, and the contents of total ash, acid-insoluble ash, ethanol soluble compounds, and leonurine were 7.87 - 10.84% (average: 9.62 ± 0.82%), 0.99 - 1.76% (average: 1.38 ± 0.24%), 16.70 - 23.11% (average: 19.49 ± 2.14%) and 0.04 - 0.17% (average: 0.11 ± 0.04%) respectively. In addition, HPLC profiling detected leonurine (5.94 min), rutin (16.43 min) and myricetin (26.78 min).Conclusions:We hope that this the rusult of the present study will contribute to the standardization and quality control of Korean herbal medicines.