양봉꿀벌은 한 마리 여왕벌을 중심으로 일벌 및 수벌들이 군집을 이루고 있는 사회성 곤충이다. 꿀벌은 여왕벌 이 깨어나면 처녀비행 (반경 2.4~7.4km )을 하여 공중에서 여러 마리의 수벌들과 교미를 한 후, 자신의 봉군 내부로 돌아와 평생을 살아가는 생태적 특성을 가지고 있다. 이와 같은 이유로 계통 증식 또는 품종 육종에서 외부의 오염원을 차단하기 위해서는 여왕벌과 수벌이 격리된 지역에서 교미가 이루어져야 한다. 본 연구는 여왕벌과 수벌이 격리될 수 있는 국내 도서지역을 중심으로 격리교미 연구를 2020년부터 2023년 봄부터 가을까지 수행하 였으며, 육종을 위한 격리교미의 효율성을 분석하고자 하였다. 도서지역은 전남 - 낙월도, 전북 - 위도, 왕등도, 식도에서 수행되었으며, 섬 크기, 경관 환경 요인, 교미 시기 등에 의해 격리교미의 성공률이 어떻게 변하는지를 확인하였다.

Marine macroalgae are important in coastal ecosystems and interact with marine microorganisms. In this study, we isolated fungi from seven types of marine macroalgae including Cladophora sp., Gloiopeltis furcate, Gracilariopsis chorda, Hydroclathrus clathratus, Prionitis crispata, Sargassum micracanthum, and Ulva lactuca collected in Korea. Morphological and phylogenetic analyses identified the isolates as four Aspergillus spp. (A. fumigatus, A. sydowii, A. tamarii, and A. terreus), three Penicillium spp. (P. crustosum, P. jejuense, and P. rubens), and Cladosporium tenuissimum. Among them, A. fumigatus TOPU2, A. tamarii SH-Sw5, and A. terreus GJ-Gf2 strains showed the activities of all enzymes examined (amylase, chitinase, lipase, and protease). Based on the enzymatic index (EI) values in solid media, A. terreus GJ-Gf2 and C. tenuissimum UL-Pr1 exhibited the highest amylase and lipase activities, respectively. Chitinolytic activity was only observed in A. terreus GJ-Gf2, A. tamarii SH-Sw5, and A. fumigatus TOP-U2. Penicillium crustosum ULCl2 and C. tenuissimum UL-Pr1 showed the highest protease activities. To the best of our knowledge, this is the first report of lipolytic and proteolytic activities in a marine-derived C. tenuissimum strain. Overall, the fungal strains isolated from the marine macroalgae in this study actively produced industrially important enzymes.

L-asparaginase (ASNase) is a therapeutic enzyme used to treat acute lymphoblastic leukemia. Currently, the most widely used ASNases are originated from bacteria. However, owing to the adverse effects of bacterial ASNases, new resources for ASNase production should be explored. Fungal enzymes are considered efficient and compatible resources of natural products for diverse applications. In particular, fungal species belonging to the genus Trichoderma are well-known producers of several commercial enzymes including cellulase, chitinase, and xylanase. However, enzyme production by marine-derived Trichoderma spp. remains to be elucidated. While screening for extracellular ASNase-producing fungi from marine environments, we found four strains showing extracellular ASNase activity. Based on the morphological and phylogenetic analyses using sequences of translation elongation factor 1-alpha (tef1α), the Trichoderma isolates were identified as T. afroharzianum, T. asperellem, T. citrinoviride, and Trichoderma sp. 1. All four strains showed different ASNase activities depending on the carbon sources. T. asperellem MABIK FU00000795 showed the highest ASNase value with lactose as a carbon source. Based on our findings, we propose that marine-derived Trichoderma spp. are potential candidates for novel ASNase production.

This study was performed to evaluate the effect of heat stress on the status of physiological responses, blood parameter, serum T3 and cortisol, and heat shock proteins (HSP 27, 70, and 90) of Hanwoo cattle. Six Hanwoo steers (242.8 ± 7.2 kg of BW) were housed in the climate-controlled respiration chambers. The experiment consisted of 7 days (control; 0 day) at thermoneutral (air temperature (Ta) of 15oC and relative humidity (RH) of 60%; temperature-humidity index (THI) = 64), and by 3 and 6 days (treatment groups) at heat stress (Ta of 35oC and RH of 60%; THI = 87). Body temperature of each parts (frank, rump, perineum and foot) and rectal temperature elevated in heat stress groups (3 days and 6 days) than the control group (0 day). Respiration rates increased in 3 days and 6 days (88.5 ± 0.96 bpm and 86.3 ± 0.63 bpm, respectively) from 0 days (39.5 ± 0.65 bpm). Feed intake significantly decreased in heat stress groups (3 days and 6 days, 3.7 ± 0.14 kg and 4.0 ± 0.15 kg, respectively) than the control group (0 day, 5.0 ± 0.00 kg). In addition, final BW significantly decreased in heat stress groups (3 days and 6 days, 211.8 ± 4.75 kg and 215.5 ± 3.50 kg, respectively) than the control group (0 day, 240.0 ± 25.00 kg). However, heat stress has no significant effect on blood parameter, serum T3 and cortisol. Nevertheless, heat stress increased HSPs mRNA expression in liver tissue, and serum concentration of HSPs. Despite Hanwoo cattle may have high adaptive ability to heat stress, our results suggested that heat stress directly effect on body temperature and respiration rate as well as serum and tissue HSPs. Therefore, we are recommended that HSPs could be the most appropriate indicators of Hanwoo cattle response to heat stress.

Korean sliced rice cakes, or tteokguk, are conventionally dried and rehydrated during their preparation. In this study, the effects of the perforation process and various drying methods (e.g., hot-air drying, vacuum drying, low temperature drying, and freeze drying) on the quality characteristics of tteokguk (rice cake soup) were evaluated. In the experiment, the rehydration capacity and lightness increased as the pore number increased. The hardness, redness, and yellowness of tteokguk, in contrast, tended to decrease as perforations increased. The texture, taste, and overall acceptability scores of tteokguk increased as perforations increased. With respect to drying methods, the rehydration capacity was greatest for vacuum drying. The hardness of tteokguk was lowest for vacuum drying. The redness, yellowness, pH, and sensory characteristics did not differ significantly among tteokguk samples treated through various drying methods. These results suggest that high-quality ready-to-eat Korean sliced rice cakes could be created by perforation and vacuum drying.

본 연구에서는 전문대학생들의 자아존중감, 자아분화수준, 스마트폰 중독의 관계를 파악하고자 하였다. 전문대학생들의 자아존중감, 자아분화수준이 스마트폰 중독에 미치는 영향을 밝히는데 목적을 두었다. 수집된 285부의 자료는 SPSS WIN 22.0 프로그램을 사용하여 분석하였다. 첫째, 전문대학생의 자아존중감, 자아분화수준, 스마트폰 중독의 일반적 특성에 대해 살펴보았다. 둘째, 전문대학생의 자아존중감, 자아분화수준, 자아존중감, 스마트폰 중독의 관계를 알아보기 위하여 상관관계분석을 실시하였다. 셋째, 스마트폰 중독에 영향을 미치는 자아존중감, 자아분화 수준의 영향력을 알아보기 위하여 중다회귀분석을 실시하였다. 연구결과, 첫째 전문대학생들의 자아존중감이 낮아질수록 전문대학생들의 스마트폰 중독 정도 가 높아진다는 것을 알 수 있었다. 둘째, 전문대학생들의 자아분화수준이 낮을수록 스마트폰 중독 정도가 높아진다는 것을 알 수 있다. 본 연구를 통하여 스마트폰으로 야기되는 문제들을 개선하고 건강하고 바람직한 스마트폰 사용 습관을 위한 프로그램의 기반이 되고자 한다.