본 논문에서는 빙하가 녹아 갈라져서 떠내려 온 새끼 펭귄이 다시 펭귄 무리로 되돌아가기 위 해서 길을 찾아 나서는 퍼즐 어드벤처 게임을 제안한다. 플레이어 40명의 로그 기록과 설문지 를 분석한 결과를 바탕으로 제안하는 게임의 특징을 살펴보고자 한다. 첫째, 제안하는 게임은 새끼 펭귄이 얼음 덩어리를 밀어서 길을 만들어 이동하는 직관적인 규칙을 제공한다. 로그 기 록에서 플레이 평균 시간은 19분이며, 이를 19개의 리스폰 지점으로 나누면, 구간별 플레이 평균시간은 1분이다. 설문에서 게임 목표 명확성은 4.45점, 게임 난이도는 4.16점을 받았다. 둘째, 제안하는 게임은 가마우지가 새끼 펭귄에게 도움말을 제시하는 등 유용한 정보를 친절 하게 제공한다. 전체 플레이 평균 시간은 baseline이 24분이고, kinder UI가 19분이다. kinder UI에서 새끼 펭귄이 얼음 덩어리를 덜 밀고, 게임 재시작을 덜 하고, 더 빨리 상호작 용하여, 문제를 빨리 해결한다. 설문에서 게임 스토리 이해는 4.15점, 유용한 정보는 4.45점을 받았다. 셋째, 제안하는 게임은 남극과 유사한 환경을 제공하여 몰입도를 상승시킨다. baseline에 비해서 kinder UI에서 다양한 게임 오브젝트와 더 적극적으로 상호작용을 시도하 고, 미션 완료 후 더 오랫동안 오로라를 지켜봤다. 설문에서 게임 캐릭터 선호도는 4.56점, 게 임 환경 만족도는 4.22점을 받았다.

상주는 귀농 도시이자 농업 중심 도시로써 농업기술원을 유치할 만큼 생태학적 연구가치가 있는 친환경 도시이다. 특히 경천섬은 낙동강 본류에 있는 상주보 인근에 위치하고 있어 낙동강 생태 환경 연구의 중요 지역 중 하나이다. 하지만, 아직 경천섬에 대한 생물다양성 연구가 부족한 상태이다. 섬과 산지의 생태환경이 다르기 때문에 생물의 다양성도 다를 것이라 간주하고, 경천섬 주변의 생태학적 가치를 재조명할 기초자료를 조사하고자 한다. 이에 경천섬과 인근에 위치한 청룡사 길목 산지에 서식하는 곤충 채집을 통해 생물다양성을 조사하여 비교분석하는 것을 목적으로 한다. 채집방법은 직접잡기, 쓸어잡기, 함정트랩, 흡충관을 이용하였으며, 함정트랩에 사용된 유인제는 소주1.8ℓ와 빙초산350㎖를 혼합하여 사용하였다. 2018년 4월~8월 중 3회 채집과정을 수행하여 목표개체수를 확보하였고, 채집된 곤충은 에탄올을 채운 conical tube에 넣어 냉동실에 보관하여 보존성을 높였다. 채집과정에서 잡은 곤충들을 건조시키고 유사종끼리 분류하고 동정하는 과정을 거쳐 다양도지수, 우점도지수, 종풍부도지수, 종균등도지수, 유사도지수를 계산할 것이다. 기존에 알려지지 않았던 경천섬 일대의 생물다양성을 세세하게 분석하는 과정을 통해 경천섬의 생태학적 가치를 드러내고, 경천섬 일대의 자연환경관리와 보존에 필요한 정보나 체계적인 관리체계를 구축할 기초자료를 제공하고자 한다.

The objectives of the present study were to examine the antioxidant activity of fractions with different isoelectric points from salmon enzymatic hydrolysates and obtain peptide fractions of sufficient amounts with higher antioxidant peptide fraction, which could be applied to the food and animal model systems. The salmon enzymatic hydrolysates were fractionated on the basis of the amphoteric nature of sample peptides by preparative isoelectric focusing without toxic solvents and reagents, which is termed autofocusing. Acidic and basic fractions showed higher 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity than the other fractions. The basic fractions showed higher hydroxyl (OH) radical scavenging activity. The weak acidic and weak basic fractions showed higher oxygen radical absorbance capacity (ORAC) values than the acidic and basic fractions. The acidic fraction showed higher metal chelating activity than other fractions. The acidic fraction suppressed lipid oxidation in the cooked patties to a greater extent than other fractions. These results suggest that peptides fractions from salmon enzymatic hydrolysate are effective antioxidant, and that autofocusing could be useful to increase antioxidant activity for application to food and animal model systems.

With advances in digital technology, retailers are utilizing digital signage which would be a visual factor of atmospherics in a retail environment. Based on stimulus-organism-response (S-O-R) model, this study was to examine a moderating effect of digital signage in relationship between atmospherics and store patronage. To collect data, a self-administered questionnaire was developed based on literatures, and mainly contained usage experience of digital signage, atmospherics (20 items), and store patronage (6 items). All items were measured on 7 point rating scale. Two sampling frames for this study were involved: experienced sample from retail stores with digital signage vs. unexperienced sample from retail stores without digital signage. Each sample was separately asked to complete the same questions regarding experience with digital signage, store atmospherics and store patronage. A total 497 usable responses (n=278 for experienced digital signage; n=219 for unexperienced digital signage) were obtained from consumers who were aged from 20 to 49 years old. For experienced sample, digital signage has been used various types: for example, location-based digital screen for store and shopping information (52.5%) was the most experienced, and followed by LED screen (28.8%) and others (e.g., Digital Look-Book, Virtual-Try-on, Interactive Screen, and Self-checking kiosk) in the fashion retail environments. Of total respondents, females (50.7%) were slightly more than were males (49.3%). For data analysis, descriptive statistics, factor analysis, t-test, hierarchy regression analysis were used by using PASW18. Preliminarily, factor analysis revealed that atmospherics consisted of three elements, such as store layout, visual aesthetics and interior display. The factor loadings were ranged from .60 to .80 and three factors were accounted for 70.42% of total variance. Compared with store without digital signage, the mean score of atmospherics (e.g., store layout, visual aesthetics, and interior display) was more highly rated in the retail stores with digital signage. In a hierarchical multiple regression model, two factors of store layout and interior display had a significant effect on store patronage in the first step (F=69.23, p<.001, Adjusted R2=.29). The store layout (β=.33, p<.001) and interior display (β=.17, p<.01) were likely to increase consumers’ store patronage. Also, interaction effect of digital signage and atmospherics was significant in the second step (R2 Change=.014, F Change=3.386, df1=3, df2=487, p<.05). Digital signage had moderating effect only in the relationship between interior display and store patronage (β=.65, p<.05). The findings suggest that digital signage plays an important role as a stimulus in improving atmospherics at the retail environments. Particularly, the digital signage would be effective in a good interior display including POP signs, product display, racks and cases, furniture, which can lead to store patronage. This study provides a managerial implication into retailers for improving atmospherics by utilizing retail technology inside a store. Especially for fashion retailers, it is required to combine digital technology service with in-store visual merchandising for a retail environment.

Some bioactive peptides have been identified by activity-guided fractionation. However, it is difficult to prepare or synthesize enough amounts of active peptides for food ingredients and for animal or human trials. The objective of the present study was to prepare peptide fractions of sufficient amounts with high antioxidant activity which could be applied to the food and animal model. Oyster hydrolysate was fractionated the basis of the amphoteric nature of sample peptides by preparative isoelectric focusing (autofocusing). To monitor the fractionation of peptides by autofocusing, amino acid analysis of the autofocusing fractions was performed. Each autofocusing fraction was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl (OH) radical scavenging activities, oxygen radical absorbance capacity (ORAC), and Fe 2+ chelatingassays. More than 40% of the peptides were distributed between Fr. 4 and 7 (pH<5.0). Approximately 16% of the peptides were recovered in basic fractions (pH>10.0; Fr. 9-10). 11 % of peptides were recovered in the neutral fraction (6.0<pH<7.0; Fr. 8). The peptides in the acidic and basic fractions are characterized by a higher content of acidic and basic amino acids, respectively. Acidic fraction showed higher ORAC values than crude oyster hydrolysate. Also, the acidic fraction showed high DPPH and OH radical scavenging activity. On the other hand, basic fraction showed higher chelating ability than crude oyster hydrolysate. In the present study, oyster peptide fractions of large-scale with high antioxidant activity were successfully fractionated by autofocusing for food additives and animal trials.

This study fabricated low thermal conductive polyacrylonitrile (PAN)-based carbon fibers containing cellulose particles while maintaining their mechanical properties. The high thermal conductivity of carbon fibers limits their application as a high temperature insulator in various systems such as an insulator for propulsion parts in aerospace or missile systems. By controlling process parameters such as the heat treatment temperature of the cellulose particles and the amount of cellulose added, the thermal and mechanical properties of the PANbased carbon fibers were investigated. The results show that it is possible to manufacture composite carbon fibers with low thermal conductivity. That is, thermal conductivities were reduced by the cellulose particles in the PAN based carbon fibers while at the same time, the tensile strength loss was minimized, and the tensile modulus increased.

송아지고기는 어린 소 즉, 송아지에서 생산되는 고기이며 국제적 기준으로 1년 미만의 소에서 생산된 고기를 통칭한다. 송아지고기는 사육방법 및 도축연령에 따라 밥빌, 대용유사양 송아지고기, 곡류사양 송아지고기 및 방목사양 송아지고기로 구분한다. 생산 및 소비량이 가장 높은 송아지 고기는 대용유사양 송아지고기로 체중 200 kg 내외, 5개월령에 도축하며 육색은 핑크색을 나타낸다. 송아지고기는 일반적인 소고기에 비해 단백질 함량이 높고 지방함량이 낮으며 불포화 지방이 다수 포함되어 있고 고기의 육질이 부드럽다. 송아지고기의 육질에 양향을 미치는 요인으로 체중, 연령, 사료조성, 사료내 철분 함량 등이 있다. 송아지고기를 생산하기 위해서는 송아지고기 생산용 대용유사료가 필요하다. 일반적으로 대용유사료내 단백질 및 지방함량이 20% 내외로 체중의 10% 이상 급여하며 조사료를 자유채식하여 스트레스를 완화하고 반추위발달을 유지하도록 한다.

The genus Glischrochilus Reitter, 1873 is characterized by reddish or yellowish markings on their elytra. This genus is belonging to family Nitidulidae Latreille,1802. Five species in this genus: Glischrochilus (Librodor) ipsoides (Reitter, 1879), Glischrochilus (Librodor) japonicus (Motschulsky, 1858), Glischrochilus (Librodor) pantherinus (Reitter, 1879), Glischrochilus (Librodor) parvipustulatus (Kolbe, 1886), Glischrochilus (Librodor) rufiventris (Reitter, 1879) have been recorded in Korea upto now. Additionally, a new recorded species, G. christophi, are recognized from korea. As the results, descriptions and illustrations of adults of totally five species with one newly recorded species in Korea were reviewed herein. A key to Korean species of genus Glischrochilus is also provided.

This study was carried out to know some regional gene difference in carabid beetle, Coptolabrus jankowskii. There are eight subspecies in this species from Korea and it was too difficult to identify by morphological similarity. The mitochondrial ND5 (NADH dehydrogenase subunit 5) gene of Coptolabrus jankowskii from four regions of (Sangju, Daegu, Mt. Jumbong and Jeju island) was compared. The results showed the differences of the base sequence of total 57 sites and the amino acid variation of the 25 sites. The Neighbor-joining tree and Maximum parsimony tree were established based on sequence data of the ND5. In NJ tree, the Jeju area except Songdangri, and Mt. Jeombong region showed close relationship group. In the case of Maximum parsimony tree, the result divided to three separated group, the first connected group was Jeju Songdangri area and the other the Korean peninsula region except Mt. Jeombong. Others were Jeju area except Songdangri, and Mt. Jeombong region.

Stem cell therapy is undoubtedly the most promising therapeutic approach for neurological disorders. Adipose tissue is ubiquitous and it can be easily harvested in large quantities under local anesthesia with little patient discomfort, making adipose tissue into the ideal large-scale source for research on clinical applications. In this study we monitored the neuronal cell differentiation potential of human adipocyte in the following condition; i) N2 medium containing 200 uM ascorbic acid (AA) and/or 10 uM flavonoid (F) and ⅱ) N2 medium containing AA and/or 10 ng/ml brain derived neurotrophic factor (BDNF) and/or, 200 ng/ml sonic hedgehog (SHH) plus 100 ng/ml fibroblast growth factor (FGF) 8. Adipose stem cells were cultured in above described differentiation condition for three weeks. RT-PCR analysis demonstrated that the mRNA levels of neuronal cell markers in differentiated adipose stem cells. Under the culture condition using N2 medium containing AA, the expression level of nestin (neural progenitor marker) m- RNA was high in all groups, while those of Neuro D, and LEP and FABP4 (adipocyte marker) mRNA were significantly decreased. Also, the addition of BDNF or SHH+FGF8 in N2 medium containing AA enhanced the neural cell differentiation from adipose stem cells, the expression level of Map2 (mature neuron) mRNA was increased, and that of TH (dopaminergic neuron marker) mRNA was high. In addition, we confirmed that the flavonoid addition has effect on the increase of Map2 expression. These results demonstrate that our designed culture condition has effect on the neural cell differentiation of adipose stem cells and this stimulatory effect may be further enhanced by transplantation.

It is known that oocytes can be activated without male contribution in vitro and develop to blastocysts which are used to isolate parthenogenetic embryonic stem (ES) cells. Differentiation capacity of the parthenogentic ES cells was rather lower than that of fertilized embryos derived ES cells, which might be the result of the absence of male genome. However, parthenogenetic ES cells might be useful research tool for genetic engineering and generating SCNT embryo derived ES cells. In our previous study, we reported that establishment of several bovine ES cell lines from in vitro fertilized (IVF) embryos named JNU-ibES. Based on this data, the objective of this study is to generate parthenogenetic ES cells and to examine their stem cell characteristics. Total 107 parthenogenetic embryos produced at day 8 or 9 were classified into their developmental stages (full expanded x 40, hatched x 67). For producing ES cells, ICM and trophetoderm-rich clumps were mechanically dissociated and were cultured on mitomycin- C treated mouse embryonic fibroblast feeder cell drop and covered with mineral oil in DMEM medium containing 20% FBS, 5 ng/ml basic FGF, 1% nonessential amino acids, and 0.55 mM b-mercaptoethanol. We obtained 20 primary parthenogenetic bovine ES (pbES)-like cell colonies. And pbES colony formation was higher in hatched blastocyst (25.4%, 16/67) than expanded blastocysts (10%, 4/40). Among those colonies, 5 pbES cell lines were successfully established and they were named as a series of JNU-pbES. These pbES cells were positively expresssed pluripotency markers such as Oct4, Nanog, TRA-1-81, SSEA-1 and alkaline phosphatase. This result demonstrated that the establishment efficiency and characteristics of pbES cell line was very similar to those of ibES cell line.

The generation of patient-specific pluripotent stem cells has the potential to accelerate the implementation of stem cells for clinical treatment of degenerative diseases. This study was to examine the in vitro neuron cell differentiation characteristics of our established human (h) iPS cells (IMR90-iPS-1~2) derived from human somatic cells. For the neuron differentiation, well grown hiPS colonies were recovered by collagenase treatment and then suspended cultured in a non-adherent bacteriological culture dish using human embryonic stem (hES) cell culture medium for 4 days. Embryoid bodies were plated and cultured in serum-free ITSFN (insulin/transferrin/selenium/fibronectin) medium for 8 days to select neural precursor cells. Then selected neuronal cells were dissociated, plated onto poly-L-ornithin/laminin coated dish at a concentration of 2 x 105 cells/cm2 and expanded in N2 medium containing 20 ng/ml bFGF, 200 ng/ml SHH and 100 ng/ml FGF-8 for 7 days. For the final differentiation step involved removing agents and culturing for 14 days in 20 ng/ml BDNF added N2 medium. In the neural precursor stage, >90% of nestin positive cells and >50% NCAM positive cells were obtained. Also, in final differentiation step, we confirmed the high percent (>80%) of mature neuron tubulin-β positive cells and approximately >20% of tyrosine hydroxylase positive cells. Also, these results were confirmed by RT-PCR. These results indicated that hiPS cells have potential to generate specific neuron differentiation and especially TH+ neuron was also can be obtained, and thus hiPS-derived neural cells might be an usable source for the study of neuro-degenerative disease.