싸리수염진딧물(Aulacorthum solani) 방제를 위해 기생성 천적으로 국내에서 선발한 목화검정진디벌(Ephedrus plagiator)과 외국에서 판매 되고 있는 진디벌(Aphidius ervi)을 비교 조사하였다. 성충 수명과 산란 기간, 머미수, 우화충수 등에 대한 우열성을 싸리수염진딧물과 완두수염진 딧물(Acyrthosiphon pisum)을 대상으로 온도 15, 20, 25, 30℃와 광주기 16(L) : 8(D)의 조건에서 조사하였다. 성충 수명은 15℃에서 목화검정 진디벌이 싸리수염진딧물에서 5.9일로 진디벌 3.6일에 비해 2.3일 길었다. 산란 기간도 15℃의 싸리수염진딧물에서 목화검정진디벌이 5.3일인 반면, 진디벌은 2.2일로 상대적으로 짧았다. 싸리수염진딧물에서 목화검정진디벌과 진디벌의 머미수는 목화검정진디벌이 15, 20, 25, 30℃에서 각각 71.5, 41.0, 15.7, 7.7개로 진디벌의 22.1 16.3 6.2, 0.4 보다 많이 기생하는 것으로 나타났다. 반면에 완두수염진딧물에서 목화검정진디벌 의 머미수는 70.3, 69.8, 34.3, 8.4개로 93.4, 71.2, 34.8, 14.5로 조사된 진디벌의 머미수보다 낮았다. 싸리수염진딧물에서 목화검정진디벌의 우 화충수는 42.1, 36, 11.6, 0로 나타나 30℃의 높은 온도에서는 우화하지 않은 것을 알 수 있었다. 진디벌의 경우에는 우화 개체수가 각 온도에서 19.6, 13.5, 3.7, 0.1로 나타나 목화검정진디벌에 비해 상대적으로 적게 우화한 것으로 조사되었다. 두 종의 비교결과, 싸리수염진딧물에서는 목 화검정진디벌이, 완두수염진딧물에서는 진디벌이 우수한 것으로 조사되었다.

진딧물 기생봉인 목화면충좀벌과 진디면충좀벌의 기주를 목화진딧물과 복숭아혹진딧물로 하여 15, 20, 25, 30℃에서 사육하면서 기주섭식수, 기생수, 우화율, 성비, 발육기간을 조사하였다. 목화진딧물이 기주일 때 15℃, 25℃에서 목화면충좀벌의 기주섭식수(5.4, 9.7마리)가 진디면충좀벌(2.0, 2.9마리)보다 많았으며 복숭아혹진딧물이 기주일 때 2종 기생봉 간 기주섭식수의 차이는 없었다. 목화진딧물에 기생한 목화면충벌의 기생수가 15℃에서 11.1마리, 30℃에서 21마리로 진디면충좀벌의 기생수(7, 12.3마리)보다 많았으며 복숭아혹진딧물에 기생시켰을 때는 20℃와 25℃에서 목화면충좀벌의 기생수(12.1, 17.1마리)가 진디면충좀벌(6.1, 10마리)보다 더 많았다. 목화진딧물을 기주로 했을 때 30℃에서 목화면충좀벌의 우화율(83.3%)이 진디면충좀벌(69.4%)보다 높았으며 복숭아혹진딧물에서는 우화율 차이가 없었다. 목화진딧물에서 사육했을 때 진디면충좀벌의 암컷비율이 15℃에서 75.2%, 30℃에서 73.9%로 목화면충좀벌의 암컷비율(19.5, 48.6%)보다 높았으며 복숭아혹진딧물에서 사육했을 때는 목화면충좀벌의 암컷비율이 15℃에서 65.3%, 25℃에서 90.0%여서 진디면충좀벌의 암컷비율(34.4, 78.8%)보다 높은 것을 알 수 있었다. 목화진딧물에서 2종 기생봉간 산란에서 우화까지의 발육기간의 차이는 없었고 복숭아혹진딧물에서는 20℃에서 목화면충좀벌의 발육기간(19.9일)이 진디면충좀벌(16.5일)보다 길었다.

Pyrifluquinazon, as a quinazinalone chemical group, based on a new mode of biological activity. It is reported that mode of action is modifies insect behavior, rapidly stopping feeding such that insects starve to death. Time-release feature and mortality effect on M. persicae using different pyrifluquinazon nano type and non-nano type were compared. Pyrifluquinazon nano type was formulated with different molecular weight and density of used chitosan (CS 30000 0.1% and CS 3000 0.3%). In the CS 30,000 0.1%, the mortality was weakly occurred at early time, but steadily increased after 4days. Finally, we confirmed more than 70% mortality as a peak at 16days. In CS 3000 0.3%, the mortality showed about 70% until 18days as a effective controlled release. Also, We examine time-release feature and mortality effect on M. persicae according to the different pyrifluquinazon nano type(CS 30000 0.1% and CS 3000 0.3%) of concentrations. The CS 30000 0.1% bioassay results of different concentration were showed that the highest concentration(100ppm) was measured better mortality than other concentration at 0 day, but cannot confirm different effect about dissimilar concentration. However, increasing rates of M. persicae were low as treatment concentrate was high. In CS 3000 0.3% 100ppm concentration bioassay result, aphid mortality reached peak at 24 days and increasing rate also low. Additionally, for the comparing of bioassay and feeding behavior of M. persicae against pyrifluquinazon nano types and non-nano type, EPG technique was carried out. In case of non nano type, feeding inhibition efficacy was showed during 4 days after treatment, but appeared similar level with control after 10days. In CS 3000 0.3% 50ppm, residual efficacy was specially showed until 28days after treatment whereas treatments with CS 30000 0.1% were similar to the control after 22days. These result show that the change of feedinng behavior and motrality of M. persicae is correlated with the change of nano type or non nano type of pyrifluquinazon.

Several species of the genus Aphidius are used in biological control programs against aphid pests throughout the world and their behavior and physiology are well studied. But despite knowing the importance of sensory organs in their behavior, their antennal structure is largely unknown. In this study, the external morphology and distribution of the antennal sensilla on the antennal of both female and male adults of A. colemani were described using scanning electron microscopy (SEM). Generally, the filaform antennae of males (1,515.20±116.48 ㎛) are longer than females (1,275.06±116.42㎛). Antennae of this species is made up of scape, pedicel and flagellomeres. Male and female antennae differed in the total number of flagellomeres as 15 in males and 13 in females. Female and male antennae of A. colemani has samely seven types of sensilla. We classified sensilla placodea, Bohm bristles, 2 types of sensilla coeloconica, , 2 types of sensilla basiconica as with a tip pore and with wall pores, sensilla trichodea. In addition, the possible functions of the above sensilla types are discussed in light of previously published literature; mechanoreception(Bohm bristles, sensilla coeloconicaⅡ and sensilla trichodea) and chemoreception(sensilla coeloconicaⅠ, sensilla basiconicaⅠ,Ⅱ and sensilla placodea). Future studies on the functional morphology of the antennal sensilla of A. colemani using transmission electron microscopy (TEM) coupled with electrophysiological recordings will likely confirm the functions of the different sensilla identified in this study.

The multicolored Asian ladybird beetle, Harmonia axyridis, is a generalist predator of aphids also, shows a high level of phenotype polymorphism in color pattern of elytra. Although, it is not sure about genetic information of color polymorphism, it has been confirmed that this phenomenon comes from their genetic traits. The color of H. axyridis elytra is mainly composed of black and red pigment. Phenoloxidase (PO) plays an important role in many insect physiological functions, i.e. sclerotization and pigmentation of cuticle and melanization of parasites. Following activation, PO catalyses the hydroxylation of tyrosine and subsequent oxidation of phenolic substance into quinines, which are further converted to melanin. However, the molecular bases of H. axyridis color pattern formation are almost unknown but it may be that the different pro-POs have different expression. In this study, total RNA samples from four each color pattern individuals, for example, succinea 1, succinea 2, conspicua and spectabilis was extracted. A cDNA enconding pro-PO was molecular cloned from each color pattern of H. axyridis and its putative amino acid sequence shared homology with pro-PO of other insects. We are pursuing to elucidate that their pro-PO sequence will be similar with those other insect PPO sequence. There are also regions of high sequence similarity, including putative activation site and two copper binding sites.

Several species of the genus Aphidius are used in biological control programs against aphid pests throughout the world and their behavior and physiology are well studied. But despite knowing the importance of sensory organs in their behavior, their antennal structure is largely unknown. In this study, the external morphology and distribution of the antennal sensilla on the antennal of both female and male adults of A. colemani were described using scanning electron microscopy (SEM). Generally, the filaform antennae of males (1,565.60± 194.64㎛) are longer than females (1,303.83±156.41㎛). Antennae of this species is made up of scape, pedicel and flagellomeres. Male and female antennae differed in the total number of flagellomeres as 15 in males and 13 in females. Female and male antennae of A. colemani has samely ten types of sensilla. We classified 3 types of sensilla trichodea as without pores, with a tip pore and with wall pores, 3 types of sensilla coeloconica, 1 sensilla placodea and 1 Bohm bristles. In addition, the possible functions of the above sensilla types are discussed in light of previously published literature; mechanoreception (Bohm bristles and sensilla coeloconica) and olfaction (sensilla trichodea and sensilla placodea). Future studies on the functional morphology of the antennal sensilla of A. colemani using transmission electron microscopy (TEM) coupled with electrophysiological recordings will likely confirm the functions of the different sensilla identified in this study.

To identify DNA markers linked to a elytra polymorphism, amplified fragment length polymorphism (AFLP) analysis was performed on DNA samples from four each colour pattern individuals (2 females and males), for example, succinea 1, succinea 2, conspicua, and spectabilis. As a result of performing AFLP analysis with the restriction endonuclease combination EcoRⅠ and Mse I, total of 2,269 AFLP fragments which were specific to succinea, conspicua and spectabilis was identified using 24 different AFLP primer combinations. Among these 2,269 fragments, 16 bands which were the most specific to one color patterns were isolated, cloned and sequenced. Subsequent UPGMA cluster analysis revealed that population of H. axyridis was divided four major group and these genetic tree showed that H. axyridis elytra colour diversity was affected by genetic polymorphism. It is considered that these genetic analyses may be facilitated the understanding of molecular genetic mechanism related with the wing colour pattern formation in this species.

As indigenous aphid parasitoid, Aphelinus varipes kill aphids for feeding in addition to parasitization. Because of this characteristic of A. varipes, this parasitoid may have the possibility of biological control agent against aphids. So we have evaluated traits such as daily paratization, total parasization, number of aphids killed by host feeding, sex ratio, development time, pupal mortality of A. varipes parasitizing green peach aphid, Myzus persicae. At 25°C and 16L:8D, longevity, total paratization and host feeding of A. varipes female was 11.0, 25.3, and 63.3 days, respectively. And development time of male and female, sex ratio (M:F), pupa mortality of offspring of A. varipes were 12.0 days, 12.5 days, 0.88, and 11.6%, respectively. However, because these results are not enough to estimate potential of A. varipes as biological control agents/factors, other factors such as host suitability (Macrosiphum euphorbiae, Aulacorthum solani), effect of temperature, and host seeking behavior of A. varipes continually will be investigated.

As an effective generalist predator of aphids and other hemipteran pests, Harmonia axyridis has been a successful biological control agent. Interestingly, it was known that there were varied in color patterns on H. axyridis elytra. In fact, Seo & Youn (2007) reported that H. axyridis had five color patterns, for example, succinea 1, 2, conspicua, spectabilis, and axyridis. But there are uncertain that H. axyridis elytra colour patterns are regulated by genetic polymorphism. So we tried to what is the reason that color patterns are greatly variable. To identify DNA markers linked to a elytra polymorphism, amplified fragment length polymorphism (AFLP) analysis was performed on DNA samples from four female succinea, conspicua, spectabilis and Coccinella septempunctata which is another species in Coccinellidae. AFLP analysis with the restriction endonuclease combination EcoRⅠ and MseⅠwas performed. Using 12 AFLP primer pairs, nine AFLP fragments which is specific between succinea, conspicua, spectabilis was identified. These nine AFLP fragments were isolated, cloned and sequenced. Subsequent UPGMA cluster analysis revealed three major group of H. axyridis populations. These genetic tree showed that H. axyridis elytra colour diversity was affected by genetic polymorphism. For more genetically understanding elytra colour genes, different primer combinations may be need to be generate enough polymorphic markers. These genetic analyses may be facilitate the understanding of molecular mechanism behind wing colour pattern formation.

The multicolored Asian ladybird beetle, Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae), is common to a wide range of natural and agricultural habitats. Applications of gamma irradiation minimized the losses of stored food and the death or failure of emergence in larval and pupal stages. On the other hand, degrade toxin waste as one of alternative to chemical pesticide for both quarantine and sprout control purposes of storage crop pest. So, we have investigated whether gamma irradiation exposed to eggs, lava, pupa and adults of H. axyridis. It may be affected the emergence, fertility, fecundity, development period and sex ratio of H. axyridis. Some changes of physiological characteristics may be applied to more efficient agents as biological control of several aphids. Insects were exposed to gamma irradiation from 0 to 500 Gy of 60Co depended on their developmental stages. The results showed that the first instar, eggs, third instar, pupae, and adults were more sensitive in order of irradiation dose. And fecundity and fertility of female adults were significantly decreased with increasing gamma irradiation dose at all tested individuals.

The multicolored Asian ladybird beetle, Harmonia axyridis, which demonstrates typical genetic polymorphism in its elytra color patterns. Early studies have color polymorphism in terms of geographical clines while a few investigated temporal populations in Coccinellidae. Nevertheless, note that geographical and temporal morph variation does not always correspond to what is expected from thermal and industrial adaption theories. A recent study of transformation and RNAi of the ladybird beetle, however, there is yet no evidence to indicate the variation is genetic or environmental factors. Here we describe a relatively new molecular fingerprinting technique, amplified fragment length polymorphism (AFLP). Because we think that color polymorphism in Coccinellidae is affected by genetic polymorphism. In total 38 markers were scored from which some markers were polymorphic. Supsequent UPGMA cluster analysis revealed 3 major group of Harmonia axyridis populations. But for strains that are more genetically similar, different primer combinations may be need to generate enough polymorphic marker.

방사성폐기물 처분 연구 사업이 법률적인 인허가 뿐만이 아니라 일반 국민의 동의를 얻기 위해서는 처분 사업의 안전성에 대한 신뢰성 획득이 중요하며 이를 위해 투명하게 공개될 수 있는 종합 성능 평가 (TSPA, Total System Performance Assessment)의 수행 이 필요하다. 본 연구에서는 처분 성능 평가의 투명성 증진을 위한 방안의 하나로 처분 종합 성능 평가 전 과정에 대해 품질 보증 원칙을 도입하여 평가 관련 전체 업무에 관한 신뢰성 향상을 꾀하고자 하였다. 이를 위해 처분 종합 성능 평가 수행의 다섯 단계인 (1) 기획, (2) 연구 수행 , (3) 문서화, (4) 내부 검토, (5) 독자적인 외부 검토 과정에 T2R3의 품질 보증 원칙을 적용한 인터넷 기반의 Cyber R&D Platform이 개발되었다. 인터넷을 기반으로 하는 본 시스템의 개발을 통해 안전성 평가 관련 모든 참여자들은 평가 전 과정에서 투명성이 유지된 데이터들에 쉽게 접근하여 이를 이용할 수 있다 Cyber R&D Platform은 안전성 평가를 위한 시나리오 개발 관련 데이터인 FEP 목록과 관련 시나리오 정보, 관련 시나리오 도출 과정 및 평가 체계 등을 체계적으로 구축한 FEAS (FEp to Assessment through Scenario development)프로그램과 안전성 평가에 필요한 입력 데이터들을 분류, 저장해 놓은 PAID (Performance Assessment Input Data) 프로그램, 그리고 이러한 자료들을 품질 보증 원칙과 절차에 의한 승인 과정을 통해 입력, 저장할 수 있는 품질 보증 시스템으로 구성되어 있으며 이를 통합 운영함으로써 도출된 데이터들의 신뢰성을 높이고자 하였다. 향후 연구에서는 Cyber R&D Platform과 평가 software와의 통합 운영으로 웹 기반 시스템에 대한 한 번의 접속만으로 안전성 평가 관련 모든 정보를 확인, 이용할 수 있도록 할 것이다.