Cold, salt and heat are most critical factors that restrict full genetic potential, growth and development of crops worldwide.. In this study, we applied an annealing control primer (ACP) based GeneFishing approach to identify differentially expressed genes (DEGs) in annual ryegrass (cv. Kowinearly) leaves under cold, salt and heat stresses. Two-week-old seedlings were exposed to cold (4°C), salt (NaCl 200 mM) and heat (42 °C) treatments for 6 h. A total 8 differentially expressed genes were isolated form ryegrass leaves. These genes were sequenced then identified and validated form National Center for Biotechnology Information (NCBI) database. We identified several promising genes encoding light harvesting chlorophyll a/b binding protein, alpha-glactosidase b, chromosome 3B, elongation factor 1-alpha, FLbaf106f03, complete genome, translation initiation factor SUI1, and glyceraldehyde-3-phosphate dehydrogenase. These genes were potentially involved in photosynthesis, plant development, protein synthesis and abiotic stress tolerance in plants. These genes might be useful for the enhancement of abiotic stress tolerance in fodder crops along with crop improvement under unfavorable environmental conditions.

The objective of this study was to investigate the dynamic neural mobilization program on the changes in muscle activity and nerve conduction velocity (NVC) in stroke patients. The participants were sampled and randomly divided into experimental group I (n=12) who underwent arm neural mobilization and experimental group II (n=13) who underwent arm dynamic neural mobilization. As the pretest, peripheral NVC of the radial, median, and ulnar nerves were measured using the Viking Quest; the biceps brachii, brachioradialis, flexor carpi radialis, and extensor carpi radialis activities were measured with sEMG. Each intervention program consisted of 10 trials per set and three sets per session. The intervention programs were performed once daily for four weeks (four days/week). Posttest measurements were taken equally as the pretest measurements. Significant differences in peripheral NVC in all sections of the radial and median nerves and wristbelow elbow and below elbow-above elbow areas of the ulnar nerve, as well as in muscle activity of all muscles except the biceps brachii. These findings indicate that dynamic neural mobilization was effective in increasing peripheral NVC and altering the muscle activity.

This study was conducted to explore the effects of seeding rate of a seed mixture on grassland productivity and botanical composition. Seeding rates were composed of four different treatments varying by the amount of seed: T1, 50% decrease in seeding rate from standard; T2, standard seeding rate; T3, 50% increase in seeding rate from standard; and T4, 100% increase in seeding rate from standard. In 2016, seasonal changes in grass botanical composition revealed T2, T3, and T4 in portion of grass ratio were the highest with a range of 87–100%, whereas T1 was the lowest of all treatments. In 2017, the botanical composition in T1 showed that the grass ratio in of the first cutting was 91%, but in the fourth cutting its ratio decreased by 75%. The results from both years were combined for each treatment, T4 (11,435 kg ha-1) and T3 (11,162 kg ha-1) demonstrated the highest dry matter yield of the treatments (T1: 8,196 kg ha-1; T2: 9,521 kg ha-1) (p＜0.05). As a result, a 50–100% increase in the seeding rate from the standard demonstrated the best grassland yield and botanical composition.

The aim of the study was to investigate the effects of incentive spirometry and Ujjayi breathing technique on the pulmonary function of smokers. Subjects were individuals who had a smoking habit of at least a year. Subjects were randomly divided into 3 groups: the incentive spirometry group (n=8), Ujjayi breathing technique (n=9), and a group applying both incentive spirometry as well as Ujjayi breathing technique (n=8). Each intervention was performed twice a day, 5 times a week, for a total of 8 weeks whereupon the change in pulmonary function was evaluated. A spirometer was used to measure FVC, FEV1, and FEV1/FVC. The survey used for this study included the Fagerström Test of Nicotine Dependence (FTND) and the Shortness of Breath Questionnaire (SOBQ). Study results for the comparison within groups showed that in the group that performed both the incentive spirometry and Ujjayi breathing technique, FEV1 improved with statistical significance (p<.05). Furthermore, within this comparison the FEV1/FVC improved with statistical significance. Comparison amongst the groups showed no statistically significant differences in all areas. Following, to effectively increase pulmonary function in young adult smokers, both incentive spirometry and Ujjayi breathing technique should be employed together.

This study was carried out to introduce of agronomic characteristics, forage yields and quality of Sorghum × Sudangrass hybrids ‘Cadan 99B’ and ‘Sweet Sioux WMR’ from 2015 to 2016 in middle and southern regions of Korea. The field experiment design was complete in seven varieties with three repetitions. Sorghum × Sudangrass hybrids were sown on mid-May in middle region, and end-May in southern region of Korea, in 2015 and 2016. The observed average heading date of Cadan 99B and Sweet Sioux WMR were July 22. The heading dates of Cadan 99B and Sweet Sioux WMR were 8 days earlier than SX-17 and 5 days earlier than brown mid-rid (BMR) Revolution. The sugar contents of Cadan 99B and Sweet Sioux WMR were 6.5 and 6.9 Brix°, respectively. Comparison with BMR variety, the sugar contents of Candan 99B and Sweet Sioux WMR were 0.2 and 0.6 Brix° higher than Revolution, respectively. The average of dry matter (DM) yield for 2 years and 2 regions of Cadan 99B (24,587kg/ha) were the highest among the seven varieties, but there was no significant difference among other varieties except headless control variety Jumbo (19,119kg/ha) and LATTE (20,778kg/ha) (p>0.05). The crude protein (CP) and in vitro dry matter digestibility (IVDMD) of Cadan 99B were 7.5% and 60.2%, and Sweet Sioux WMR were 6.9% and 60.7%, respectively. The results of this study indicated that Sorghum × Sudangrass hybrids Cadan 99B and Sweet Sioux WMR are earlier heading dates and higher than SX-17, and high yields of DM in middle and southern regions of Korea.

Drought is one of the detrimental factors that impair plant growth and productivity. In this study, we applied annealing control primer (ACP)-based reverse transcriptase PCR (polymerase chain reaction) technique to identify differentially expressed genes (DEGs) in maize leaves in response to drought stress. Two-week-old maize seedlings were exposed to drought (DT) by suspending water supply. DEGs were screened after 3 days of DT-treated samples using the ACP-based technique. Several DEGs encoding 16.9 protein, antimicrobial protein, hypothetical protein NCLIV_068840, thioredoxin M-type were identified in maize leaves under drought stress. These genes have putative functions in plant defense response, growth and development. These identified genes would be useful for predictive markers of plant defense, and growth responses under drought stress in plants.

Salt stress is one of the most limiting factors that reduce plant growth, development and yield. However, identification of salt-inducible genes is an initial step for understanding the adaptive response of plants to salt stress. In this study, we used an annealing control primer (ACP) based GeneFishing technique to identify differentially expressed genes (DEGs) in Italian ryegrass seedlings under salt stress. Ten-day-old seedlings were exposed to 100 mM NaCl for 6 h. Using 60 ACPs, a total 8 up-regulated genes were identified and sequenced. We identified several promising genes encoding alpha-glactosidase b, light harvesting chlorophyll a/b binding protein, metallothionein-like protein 3B-like, translation factor SUI, translation initiation factor eIF1, glyceraldehyde-3-phosphate dehydrogenase 2 and elongation factor 1-alpha. These genes were mostly involved in plant development, signaling, ROS detoxification and salt acclimation. However, this study provides new molecular information of several genes to understand the salt stress response. These genes would be useful for the enhancement of salt stress tolerance in plants.

This study was conducted to find out the effects of over seeding using new grass varieties orchardgrass ‘Kordione’ and ‘Onnuri’, and tall fescue ‘Greenmaster’ on botanical composition and dry matter productivity from 2013 to 2016 in low productivity hilly pasture, middle area of Korea. There were used 3 grass seed mixture types made of different compositions and amounts {T1: control - no over seeding, T2: tall fescue (TF) ‘Greenmaster’ 18 kg ha-1, orchardgrass (OG) ‘Kordione’ 9 kg ha-1, Perennial ryegrass (PRG) ‘Linn’ 5 kg ha-1, and Kentucky bluegrass (KBG) ‘Kenblue’ 2 kg ha-1, T3: TF ‘Greenmaster’ 9 kg ha-1, OG ‘Kordione’ 18 kg ha-1, PRG ‘Linn’ 5 kg ha-1, and KBG ‘ Kenblue’ 2 kg ha-1, T4: TF ‘Greenmaster’ 9 kg ha-1, OG ‘Onnuri’ 18 kg ha-1, PRG ‘Linn’ 5 kg ha-1, and KBG ‘Kenblue’ 2 kg ha-1}. In the botanical composition of grassland, T1 nearly remained constant in other seasons while ratio of weeds were increased in summer season. T2 was better than control (T1) in portion of grass ratio, which has increased by 80%. In the early time of establishment, the portions of OG were increased in T2, but TF rate was increased after 3 years later when grass was established. T3 and T4 showed a very similar patterns, grass ratio had increased by 80% and the portion of KBG had increased as time passed. T2 (129,763 kg ha-1) was showed the highest dry matter yield than other treatments (T1: 6,756 kg ha-1, T3: 9641, and T4: 10,738) in 2016.

This experiment was carried out to compare the growth characteristics and productivity between sudangrass and sorghum-sudangrass hybrid under the same cultivation condition in Cheonan and Kimje of Korea from 2010 to 2011. Sudangrass hybrids(Choice, Sunstar and Piper) and sorghum-sudangrass hybrids(SX-17, Jumbo and Revolution) were planted 5th May in Cheonan and 7th May in Kimje. Heading date of sudangrass hybrids was 12th July which was about 2 week fast more than that of sorghum-sudangrass hybrids. Stem diameter of sudangrass hybrids was about 6mm which was about 5 mm smaller than that of sorghum-sudangrass hybrids. Disease resistance of sudangrass hybrid, “Choice” was similar to that of sorghum-sudangrass hybrids. Dry matter yield of “Choice” was 15ton/ha which was 10% less than that for sorghum-sudangrass hybrids(SX-17). Feed value of “Choice” was similar or slightly lower than that of sorghum-sudangrass. For increasing the productivity in forage cropping systems all around year, sudangrass hybrid is recommended for prolong by the middle ten day of May in the harvesting time of italian ryegrass

This experiment was carried out to study the effect of rice black-streaked dwarf virus(RBSDV) infection rate on forage productivity of corn varieties in Cheonan of chungcheongnamdo from 2006 to 2008. Forage corn varieties of 10 were cultivated with first cropping(seeding in the last ten days of April) and second cropping(seeding in the last ten days of May) system in field and tested the infection rates of RBSDV and productivity of forage. The Infection rate of RBSDV was significant difference between corn varieties in middle district of Korea. Resistant corn varieties for RBSDV were ‘Kwanganok’, ‘P3156’, ‘Kwangpeyongok’ and ‘P3394’ but susceptible varieties were ‘Suwon19’, ‘DK697’, ‘GW6959’ and NC+7117. Dry matter(DM) yield of forage corn according with infection rates of RBSDV in field was significant difference between varieties(p<0.05). DM yield of susceptible varieties, ‘Suwon 19’, ‘DK697’ and ‘GW6959’ was lower about 20% than that of resistant varieties, ‘Kwangpeyongok’ and ‘P3156’. For increasing the productivity of forage corn, recommend of resistant varieties for RBSDV and control of seeding time are very important in middle district of Korea.

This experiment was carried out to investigate the effect of harvesting time of Italian ryegrass (Lolium multiflorum Lam; IRG) in spring on dry matter (DM) yield. IRG cultivars ‘Kowinearly’ and ‘Greenfarm’ were seeded at 50 kg/ha and grown on rice paddy fields. There was no difference in growth characteristics between both varieties before winter. However, cold tolerance of Kowinearly was higher than that of Greenfarm, as demonstrated by post-wintering growth characteristics. The heading date of both varieties was affected by the spring weather. The IRG was harvested three times at 5-day intervals beginning from 9 May. The DM yield of Greenfarm was 6,306; 7,335; and 8,109 kg/ha, and that of Kowinearly was 7,498; 9,196; and 10,449 kg/ha at the three consecutive harvests. The delay of the harvesting time for 5 and 10 days increased the DM yield of Greenfarm by 16% and 29% and that of Kowinearly by 23% and 39%, respectively, compared to the yield at first harvest (p < 0.05). Therefore, IRG harvest later than early to mid-May is expected to increase productivity. The feed values of Greenfarm were: 12.2% of crude protein (CP), 34.5% of acid detergent fiber (ADF), 57.7% of neutral detergent fiber (NDF), 61.6% of total digestible nutrients (TDN), and 72.3% of in vitro DM digestibility (IVDMD). For Kowinearly, these values were 16.4% of CP, 30.4% of ADF, 52.7% of NDF, 64.9% of TDN and 79.0% of IVDMD.

The present study investigated the effects of different seeding rates on growth characteristics and seed productivity of the “Kowinearly” cultivar of Italian ryegrass (Lolium multiflorum Lam.) in paddy fields. Sowing Kowinearly in paddy fields under growing rice at the rates of 20, 30, 40, and 50 kg/ha resulted in seed yields of 1.57, 1.92, 2.06, and 2.09 ton/ha, respectively. Seed yield of Kowinearly was the highest at a seeding rate of 50 kg/ha (p<0.05), at which the cultivar was able to survive in winter and the weed ratio was low. Under these conditions, most growth characteristics such as winter survival (85%), weed ratio (10%), stems per square meter (1,006) were superior than those sown at other seeding rates (p<0.05). In addition, it has been reported that the economic efficiency of the 50 kg/ha seeding rate was higher than that of the other seeding rates.

The objective of this study was to determine the effect of harvesting time of rice on growth characteristics and dry matter yields of Italian ryegrass (Lolium multiflorum Lam., IRG) ‘Kowinearly’ after seeding them in paddy field. Experiments were performed in National Institute of Animal Science, RDA (Cheonan, Choongcheongnam-do province of Korea) for four years (2011~2014). Italian ryegrass variety ‘Kowinearly’ was seeded at experimental paddy fields before rice harvest in late September. Rice were harvested at 10, 20, and 30 days after seeding of IRG. As the harvest time of rice became later, the number of tillering before winter was reduced and IRG plant length before winter was shorter. The dry matter yield of IRG was also reduced. When rice were harvested at 20 days and 30 days after sowing of IRG compared to the rice harvested in autumn at 10 days after sowing of IRG, the number of tillering for IRG before winter was decreased by an average of 19% and 40%, respectively. The winter survival ratio of IRG was decreased by an average of 13% and 19%, respectively. Their dry matter yields were also reduced by an average of 8% and 19%, respectively. The heading date was different from each other in three years. The plant length at the time of IRG harvest in the spring was not significantly different among treatment groups. Taken together, our results suggest that when Italian ryegrass is grown in paddy fields by the method of ‘seeding of IRG before rice harvest’, rice should be harvested as soon as possible after sowing of IRG ‘Kowinearly’ in the autumn to increase forage productivity.

This study was conducted to evaluate the growth characteristics and dry matter yields of Italian ryegrass (Lolium multiflorum Lam., IRG) cultivars for three years (2011~2013). The cold-resistance of domestic cultivars was stronger than other foreign varieties. Among early-maturing cultivars, dry matter (DM) yield of ‘Kowinearly’ was significantly higher (8,751 kg/ha) than other cultivars (p<0.05). In medium-maturing cultivars, DM yield of ‘Kowinmaster’ and ‘Tam 90’ was 9,394 and 7,461 kg/ha, respectively. Of the two, the former’s DM yield was higher than the latter’s (p<0.05). Among late-maturing cultivars, DM yield of ‘Hwasan 104’ (9,809 kg/ha) was similar to ‘Hwasan 101’ (9,354 kg/ha), but significantly higher than the other late-maturing cultivars (p<0.05). According to the results obtained from present study, early-maturing cultivars with cold-tolerance and high productivity is required for the paddy field cultivation of an Italian ryegrass cultivar after rice harvest in Cheonan region. Medium- and late-maturing cultivars are more advantageous for the dry field cultivation of the maximum quantity that is possible. In conclusion, to select which cultivar/s to grow, the climate of the growing area, weather conditions, and the cropping system must all be considered

Arsenic (As) is a toxic element that easily taken up by plants root. Several toxic forms of As disrupt plant metabolism by a series of cellular alterations. In this study, we applied annealing control primer (ACP)-based reverse transcriptase PCR (polymerase chain reaction) technique to identify differentially expressed genes (DEGs) in alfalfa roots in response to As stress. Two-week-old alfalfa seedlings were exposed to As treatment for 6 hours. DEGs were screened from As treated samples using the ACP-based technique. A total of six DEGs including heat shock protein, HSP 23, plastocyanin-like domain protein162, thioredoxin H-type 1 protein, protein MKS1, and NAD(P)H dehydrogenase B2 were identified in alfalfa roots under As stress. These genes have putative functions in abiotic stress homeostasis, antioxidant activity, and plant defense. These identified genes would be useful to increase As tolerance in alfalfa plants.

Copper (Cu) is a necessary microelement for plants. However, high concentrations of Cu are toxic to plants that change the regulation of several stress-induced proteins. In this study, an annealing control primer (ACP) based approach was used to identify differentially expressed Cu-induced genes in alfalfa leaves. Two-week-old alfalfa plants (Medicago sativa L.) were exposed to Cu for 6 h. Total RNAs were isolated from treated and control leaves followed by ACP-based PCR technique. Using GeneFishing ACPs, we obtained several genes those expression levels were induced by Cu. Finally, we identified several genes including UDP-glucuronic acid decarboxylase, transmembrane protein, small heat shock protein, C-type cytochrome biogenesis protein, mitochondrial 2-oxoglutarate, and trans-2,3-enoyl-CoA reductase in alfalfa leaves. These identified genes have putative functions in cellular processes such as cell wall structural rearrangements, transduction, stress tolerance, heme transport, carbon and nitrogen assimilation, and lipid biosynthesis. Response of Cu-induced genes and their identification in alfalfa would be useful for molecular breeding to improve alfalfa with tolerance to heavy metals.

This study was conducted to determine grazing intensity of growing Korean native goats (Capra hircus coreanae) on mountainous pasture. It was carried out to obtain basic information for improvement of mountainous pasture management and establishing feeding system of Korean native goats. Castrated, male goats (n=10) with average initial body weight (BW) of 23.33±2.15kg and an average age of 4 months were used in this study. Grazing goats were supplemented by concentrates with 1.0% of BW. The crude protein content of forage was the highest in October (22.71±0.25%) and there were significantly differences (p<0.05) in monthly comparison. The forage productivity of pasture was the highest from May to June (1718.7±207.5~ 1672.0±422.8 kg/ha) but it was decreased in July (1356.0±103.8 kg/ha) because of drought and summer depression. Average daily gains (ADG) of goats were the highest in June (99.5±6.4 d/g). Grazing intensity was calculated by forage productivity and dry matter intake (DMI) and was the highest in May (65 head/ha). As shown in the results of this research, grazing intensity was suggested to average 39 head/ha from May to October. It is desirable that adequate grazing intensity was maintained by adjusting supplemental feed.