본 연구는 제주지역에서 난지형 목초의 도입을 위한 버뮤다그라스와 바히아그라스의 생육특성 및 사초생산성을 평가하기 위하여 국립축산과학원 난지축산시험장 조사료 시험포장 (해발 200 m)에서 2007년 4월부터 2009년 12월까지 수행하였다. 난지형 영년생 화본과 목초인버뮤다그라스 “Common”, “제주 자생형 (Ecotype)”품종과 바히아그라스 “Argentine”, “Tifton 9”품종을 2007년 4월 22일에 파종하였다. 버뮤다그라스의 출현일은 5월 8일로 파종 후 16일정도 소요되었으며 바히아그라스는 파종 후 24~28일 정도 소요되는 것으로 나타났다. 출수기는 버뮤다그라스 Common은 5월 28일, 바이하그라스 Tifton 9은 7월 4일에 출수기에 도달하였다. 버뮤다그라스의 3년간 평균 건물수량은Common이 16,749 kg/ha으로 Ecotype 보다 다소높게 나타났으며 바히아그라스에 있어서는Tifton 9이 23,695 kg/ha으로 Argentine 보다 높게 나타났다. 조단백질 함량은 버뮤다그라스의Ecotype이 13.86%로 가장 높게 나타났으며ADF 함량은 버뮤다그라스 common이 27.97%로 가장 낮은 것으로 나타났다. 총 가소화영양소 (TDN) 함량은 버뮤다그라스가 65.48%로 바히아그라스 보다 높은 것으로 나타났으며 invitro 건물소화율은 각각 70.90%, 70.44%로 두초종이 모두 비슷한 경향을 보였다. 이상의 결과를 종합해 볼 때 제주지역에서 버뮤다그라스와 바히아그라스는 월동이 가능하여 여름철조사료자원으로 활용이 가능하고 또한 지하경과 포복경을 가지고 있어 말의 제상에 강한 특성을 가지고 있어 여름철 방목용 초종으로 적합한 것으로 판단된다.

본 연구는 전남 신안군 비금도 간척 논에서 백수현상이 나타난 벼를 조사료 자원으로 활용하기 위해 백수 벼 사일리지의 사료가치 및 유기산 등 품질을 조사하기 위해서 수행하였다.벼 백수현상이 가장 심한지역의 총체 벼의 수량은 정상지역보다 약 800 kg/ha 정도 감소되었다. 그리고 벼 백수현상이 가장 심한지역에서의 총체 벼의 조단백질 함량 및 TDN 함량은벼 백수현상이 없는 정상생육 지역 보다 약간높은 함량을 보였으나 ADF 및 NDF 함량은 현저하게 감소되었다 (p<0.05). 벼 백수현상이 가장 심한지역과 정상지역의 총체 벼 사일리지pH는 4.0~5.0을 나타냈으며 젖산균 접종에 의해 pH가 저하되었다. 벼 백수지역과 정상생육지역 모두 젖산균 접종에 의해 젖산함량은 증가하였으며 초산과 낙산함량은 감소되었다(p<0.05). Flieg법에 의한 총체 벼 사일리지의품질등급은 젖산균을 접종함으로써 2~3단계 상승되었다. 따라서 본 연구의 결과에서 나타난바와 같이 백수현상이 나타난 벼도 조사료원으로 활용될 수 있다.

본 시험은 2007년부터 2008년까지 2년간에 걸쳐 충남 천안, 경북 영주, 전북 김제에서 여름 사료작물인 사일리지용 옥수수를 파종하여국내 육성품종인 광평옥과 도입품종간의 생육특성 및 수량성에 대한 기초자료를 얻고자 실시하였다.연구결과 도입품종 사일리지용 옥수수 중에서 Cap444NG 품종이 녹색도가 우수하고 생초수량이 61,111 kg/ha이고 건물수량이 20,635kg/ha로 최고의 수량을 보였다. 또한 사료가치에 있어서도 조단백질 함량이 7.9%로 양호한생육특성을 보였다.

Gene manipulation of Siberian wildrye grass through transgenic technology can modify grass feature for further improvement in the forage production. In order to optimize transformation conditions of Siberian wildrye grass, the effects of transformation efficiency was investigated with GUS gene. Seed-derived calli were infected and co-cultured with Agrobacterium tumefaciens carrying the binary vector pCAMBIA3301 encoding the GUS gene in the T-DNA region. The effects of several factors on transformation and the expression of the GUS gene were investigated. The highest transformation efficiency was obtained when embryogenic calli were inoculated with Agrobacterium strain EHA101 in the presence of 200 μM acetosyringone and coculture for 5 days. The present study was framed and materialized to standardize an efficient, high frequency and reproducible genetic transformation protocol for aromatic Siberian wildrye grass using Agrobacterium tumefaciens.

To get good productivity and high feed value of forage, we develop to new Italian ryegrass (Lolium multiflorum Lam.) 'Kogreen'.variety. In Suwon, heading date of 'Kogreen' was on May 3 but in Yonchun was on 8 May that was 2 day later than that of 'Florida 80' and dry matter yield is 12.7ton/㏊, and had a good cold tolerance.

In the present study, we have developed a high-frequency plant regeneration system for Italian ryegrass via callus culture using mature seeds as explants. Optimal embryogenic callus induction was found to occur in MS medium containing 5 ㎎ 1?¹ 2,4-D, 0.5 ㎎ 1?¹ BA, 500 ㎎ 1?¹ L-proline, 1 g 1?¹ casein hydrolysate, 30 g 1?¹ sucrose, 7 ㎎ 1?¹ AgNO₃, 2 ㎎ 1?¹ CuSO₄ and solidified with 3 g 1?¹ Gelrite. The highest regeneration rate was obtained in MS medium containing 1 ㎎ 1?¹ 2,4-D, 5 ㎎ 1?¹ BA, 500 ㎎ 1?¹ L-proline, 1 g 1?¹ casein hydrolysate, 1 ㎎ 1?¹ thiamine-HCl, 30 g 1?¹ sucrose, 7 ㎎ 1?¹ AgNO₃, 2 ㎎ 1?¹ CuSO₄ and solidified with 3 g 1?¹ Gelrite. By using the most effective treatment determined for each parameter, the highest rates of embryogenic callus formation (48.9%) and regeneration (47.6%) were obtained with the Hwasan 101 cultivar. The overall plant regeneration rates of the examined cultivars ranged from 7.5% to 23.2%. Thus, optimization of regeneration frequency using mature seeds as explant material may offer a simple and efficient protocol for Italian ryegrass that may improve molecular breeding of this species.

In the present study, genotypic variation of Agrobacterium-mediated transformation of Korean Italian ryegrass has been evaluated. Mature seed-derived calli of a total of seven cultivars were infected and co-cultured with Agrobacterium tumefaciens carrying the binary vector pCAMBIA1301, which contains a reporter gene (gus) and a plant selectable marker gene conferring resistance to hygromycin (hpt) in the T-DNA region. The effects of several factors such as callus type and callus age on transformation frequency and the expression of the GUS gene were investigated. The highest transformation frequency (6.7%) was obtained with the Hwasan 101 cultivar when 9-week-old calli (type-I) were inoculated with Agrobacterium. The overall transformation rates of the examined cultivars ranged from 0.4% to 6.7%. GUS histochemical assays, PCR, and southern analysis of transgenic plants demonstrated that transgenes were successfully integrated into the genome of Italian ryegrass. Thus, optimization of transformation frequency and selection of a suitable cultivar of Italian ryegrass may improve molecular breeding of this species.

Perennial ryegrass (Lolium perenne L.) is one of the most important grass species in the world's temperate zones. It is used as high-quality forage in pastures and for recreational use as turf in golf courses, lawns and parks. Genetic improvement of perennial ryegrass is difficult due to its self-incompatibility. Consequently, progress by conventional breeding can be slow. Genetic transformation is an alternative that permits direct introduction of useful genes into a plant's genome and is becoming a powerful tool to complement conventional breeding. To improve environmental stress tolerance and quality of perennial ryegrass by introducing better and useful genes into the genome, we have developed a rapid and efficient transformation system using Agrobacterium-mediated gene transfer system.

In the present study, we have used an annealing-control-primer (ACP)-based differentially display RT-PCR method to identify salt-stress-induced differentially expressed genes (DEGs) in barley leaves. Using 120 ACPs, a total of 11 up-regulated genes were identified and sequenced. Temporal expression patterns of some up-regulated DEGs in response to salt stress were further analyzed by Northern blot analysis. The possible roles of these identified genes are discussed within the context of their putative role in response to salt stress. Thus, the identification of some novel genes-such as SnRK1-type protein kinase; 17 kDa, class I, small heat shock protein; and RNase S-like protein precursor genes-may offer a new avenue for better understanding the salt stress response in plants, knowledge which might be helpful for developing future strategies.

Abiotic stress is the major limiting factor of forage crops growth and yields. The objective of this work was to study the stress tolerance and regeneration capability of transgenic forage crops carrying a MsHSP23 gene, encoding a alfalfa mitochondrial sHSP protein. The expression of the MsHSP23 gene was confirmed in bacteria, recombinant mHSP23 conferred tolerance to salinity and arsenic stress. Furthermore, mHSP23 was cloned in a plant expressing vector and transformed into forage crops such as alfalfa, tall fescue and bent grass. The transgenic plants exhibited enhanced tolerance to salinity and arsenic stress conditions. In comparison to wild type plants, transgenic plants were exhibited significantly lower electrolyte leakage. Moreover, the transgenic plants had superior germination rates when placed on medium containing arsenic. Taken together, these overexpression results imply that mHSP23 plays an important role in salinity and arsenic stress tolerance in transgenic forage crops. This approach could be useful to develop stress-tolerant plants including forage crops.

The molecular responses to various abiotic stresses were investigated by the approaches with transcriptomic analysis based on an ACP system. Here we identified differentially expressed genes under abiotic stresses in alfalfa seedlings and they were mostly unknown genes and a few common stress-related genes. Among them, mitochondrial small HSP23 was responded by the diverse stress treatment such as heat, salt, As stresses and thus it could be a strong candidate that may confer the abiotic stress tolerance to plants. When expressed in bacteria, recombinant MsHSP23 conferred tolerance to salinity and arsenic stress. Furthermore, MsHSP23 was cloned in a plant expressing vector and transformed into tobacco, a eukaryotic model organism. The transgenic plants exhibited enhanced tolerance to salinity and arsenic stress under ex vitro conditions. In comparison to wild type plants, the transgenic plants exhibited significantly lower electrolyte leakage. Moreover, the transgenic plants had superior germination rates when placed on medium containing arsenic. Taken together, these overexpression results imply that MsHSP23 plays an important role in salinity and arsenic stress tolerance in transgenic tobacco. The results of the present study show that overexpression of alfalfa mitochondrial MsHSP23 in both eukaryotic and prokaryotic model systems confers enhanced tolerance to salt and arsenic stress. This indicates that MsHSP23 could be used potentially for the development of stress tolerant transgenic crops, such as forages.