To find an alternative for synthetic pesticides, methanol extract from plant samples were tested for their insecticidal activity against insect. The extract of Asiasarum sieboldii had strongly insecticidal activity against Plutella xylostella. Roots of A. sieboldii were extracted with methanol, and the concentrated extract was partitioned with n-hexane, ethylacetate, n-buthanol and H2O. The highest activity was shown in the hexane fraction. Activity-guided fractionation led to the isolation of two amides from hexane fration through the repeated silica gel column chromatographic separations. From the interpretation of spectropic data including NMR, MS, IR, the chemical structures of compounds were determined as dodeca-2E,4E,8Z,10Z-tetraenoic acid isobutylamide and dodeca-2E,4E,8Z, 10E-tetraenoic acid isobutylamide. These compounds showed insecticidal activity on P. xylostella by 96.7% at 100ppm. The liquid formulation controlled on cabbage effectively. The extract and compounds from A. sieboldii showed insecticidal activity against Nilaparvata lugens. As a naturally occurring pesticide, A. sieboldii could be useful as a new botanic insecticide.

Background : This study examines the main growth characteristics, physiological stress, or diseases damage for 3 and 4-years-old ginseng in the new lines cultivated in Gyeonggi for development of new lines of Ginseng. Methods and Results : Eumseong 13, 14 and 15, which are two-years-old seedling in the new line ginseng were transplanted in 7 lines × 9 rows (70 plants)/1.62㎡ in 2015 along with Gumpoong and Yeonpoong as the control lines while Eumseong 16 and 17 as well as Chunpoong as the control line were transplanted in the same way in 2016. The ginseng shading was installed with the front post of 170 ㎝ high and the rear post of 100 ㎝ high using the rear line while as the sun blocking material, the aluminum shade plate was used. As for the new growth lines of 3 and 4-years ginseng in the new growth line, the aerial parts of the ginseng and underground part of ginseng were examined. In addition, the physiological stress such as the leaf discoloration, rusty root and root rot as well as the resistance against the disease were examined. It was found out that as for the aerial parts of 3-years-old ginseng in test line, Eumseong 16 and 17 were better in the growth than the control lines were while in 4 years old ginseng, Eumseong 13 and 14 were better in the growth than Gumpoong as the control line was. As for the root weight of the 4-years-old ginseng for the underground part, there was no significant difference compared with control line while in 3-years-old ginseng, Eumseong 16 and 17 were heavier. The leaf discoloration did not happen to both 3 and 4-years-old ginseng. As for the root rot, 3-years-old Eumseong 16 and 17 suffered less root rot in the same way as for the control line. 4-years-old Eumseong 14 and 15 had less root rot in the same way as for the control line. 3-years-old Eumseong 16 and 17 have less rusty root in the same way as the control line while as for 4-years-old ginseng, Eumseong 13, 14 and 15 showed a lot of rusty roots in the same way as the control line. Conclusion : Finally, considering the growth characteristics, physiological stress and root rusty of the aerial parts and underground parts of the ginseng, we selected Eumseong 16 and 17 as the excellent lines for 3-years-old ginseng.

Background : This study was conducted to select a optimal combination of LED light sources for stable production of sprout ginseng used as vegetables throughout the year. Methods and Results : The treatments to select the optimum LED light source for the production of functional sprout ginseng were ①Blue40%: Green40%: Red40% (B40 + G12 + R20 umol·m-2S-1), ②B50: G25: R10 (B50 + G8 + R5 umol·m-2S-1), ③B50: G20: R40 (B50 + G6 + R20 umol·m-2S-1), ④B35: G20: R35 (B35 + G6 + R18 umol·m-2S-1), ⑤B20: G0: R40 (B20 + R20 umol·m-2S-1), ⑥control (a general fluorescent lamp for plant cultivation, FL40W, PG, 35.0 umol). The ginseng seedlings used in the test were subject to preconditioning process for one week after the cold treatment at 4℃ for at least three months. The root media were composed of porous artificial soil 40% + peat moss 30% + perlite 20% + Vomito (brand name)10%. We planted 35 seedlings (planting density 5 × 7 ㎝) in a 480 × 380 × 295 ㎜ plastic box. The two liters of water were irrigated per a plastic box when water potential reached -33 kPa using a tensimeter. The cultivation room was maintained at 25℃ with 45% of the relative humidity, and 16 hours of lighting for growing conditions. The growing period was 40 to 60 days after planting. The results were as follows; the treatment of LED B50%: G25%: R10% (B50 + G8 + R5 umol·m-2S-1) increased the diameter of the sprout ginseng by 10.3% (4.95 → 5.46 ㎜), root weight 27.3% (1.1 → 1.4 g), fresh weight 38.9% (1.8 → 2.5 g) in comparison with the control. The contents of ginsenoside such as Re, Rg2, Rc, Rb2, and Rd increased 24.2% (24.8 → 30.8 ㎎/g). Conclusion : The optimal light source for sprout ginseng was LED B50%: G25%: R10% (B50 + G8 + R5 umol·m-2S-1), which increased sprout production by 38.9% in comparison with the control.

Background : This study researches on the microclimate, photosynthesis and growth characteristics for the development of shading materials proper for the wide and inclined ginseng cultivation facility which can respond to climate change and save labor. Methods and Results : The wide shading facilities were installed on the area of 1,000 ㎡ in 2014 and 4 facilities were installed on the test ginseng cultivation area. On Mar. 29, 2017, 2 blue shading nets [with the sun blocking rate of 85% (200 g/㎡) and 90% (220 g/㎡)] were installed each for 4 facilities. On June 26, 2017, the aluminum screen and black shading net (with the sun blocking rate of 40% each) were installed during the period of high temperature (30℃ or higher) for each facility. The maximum light intensity under the shading facility was high with blue shading net 85% and PE black shading net 40%, or blue shading net 85% and aluminum screen 40%, higher than other treatments. They were higher by 4.8 - 7.3%, 5.3 - 7.8% each in July and August. Among the coating materials for reducing the high temperature, the aluminum screen coating had less water leakage in early July, late July, mid-August and late August when the precipitation was more than 100 ㎜. The death of the aerial part of ginseng occurred less until October. The growth of the aerial part of 4-year ginseng was better in blue shading net 85% and PE black shading net 40%, blue shading net 85% and aluminum screen 40% or blue shading net 90% and PE black shading net 40% than in blue shading net 90% and aluminum screen 40% The photosynthesis rate was the highest in June with 3.67 μmol CO2/㎡/s under the blue shading net 90% and aluminum screen 40% and with 3.55 μmol CO2/㎡/s under blue shading net 85% and PE black shading net 40% for K-1. As for the land races ginseng, it was the highest with 3.55 μmol CO2/㎡/s under the blue shading net 85% and PE black shading net 40%. For the growth of the underground part of the 4-year ginseng, the blue shading net 85% + PE black shading net 40% or the blue shading net 85% + aluminum screen 40% was the best with respect to the growth of the ground part of the ginseng such as the length of root, the length of main root, diameter of root and weight of root than other treatment. Conclusion : Best coating materials for the wide shading facilities are the blue shading net 85% and aluminum screen 40%.

Background : This study is designed to select the best shading materials for the 4-year old ginseng K-1 variety by examining the light volume, photosynthesis and growth characteristics of ginseng. Methods and Results : The ginseng seedling was transplanted in the 7 lines x 9 rows (63 plants)/ 1.65 ㎡. The shading materials were installed in the form of rear line with 170 ㎝ high for front pole and 100 ㎝ high for rear pole. The installation of shading materials were installed in combination of polyethylene, blue polyethylene sheet + polyethylene, polyethylene + polyethylene, polyethylene + aluminium screen 70%, polyethylene + aluminium screen 100%, aluminium shade plate to inspect the photosynthesis and ginseng's growth characteristics. The photosynthetic rate was measured on the middle of small leaf using the photosynthetic tester (L1-6400, Li-COR) a t 10 : 00 – 14 : 00 on a clear day with the light volume of 100, 200, 400, 600, 800, 1,000, 1,400, 1,800 μmol s-1m-2.For the ginseng K-1, which is 4-years old, the aerial parts growth (such as survival rate, height of plant, length of stem, diameter of steam and length of leaf) and underground part (length, diameter and weight of root) were examined in 2016. As for the PAR (photosynthetically active radiation), the aluminum shade plate showed the best performance while the polyethylene + polyethylene showed the least. As for the photosynthetic rate, the following order was shown in the decreasing order: blue polyethylene sheet + polyethylene > polyethylene > aluminium shade plate ≒ polyethylene + aluminium screen 100%, > polyethylene + polyethylene > polyethylene + aluminium screen 70%. As for the survival rate for 4-year old ginseng, the polyethylene + aluminium screen 70% showed the highest survival rate with 91.0%. As for the growth of aerial part of ginseng, the polyethylene and polyethylene + polyethylene showed the best growth. As for the underground part of ginseng, the polyethylene, blue polyethylene sheet + polyethylene, and polyethylene + polyethylene showed the best growth in the length of roots and length of main root while polyethylene and blue polyethylene sheet + polyethylene showed the best growth in the weight of roots. Conclusion : In conclusion, considering the characteristics of photosynthesis and the underground parts of ginseng, it was found that the best shading materials for K-1 4-year old ginseng is blue polyethylene sheet + polyethylene.

Background : The study is designed to select the organic fertilizer as an additional fertilizer suitable for improving the yield of ginseng cultivar K-1 at low productive land when it is cultivated as 6-year old red ginseng. Methods and Results : This study was conducted in 2016 at a farm in Paju cultivating 6-year old ginseng root. As for the management of the cultivation land, in 2010 and 2011, Sudangrass was cultivated in order to make the organic matter content and chemical composition suitable for ginseng cultivation, and more than 15 tillage works were carried out a year. The transplantation was carried out at the planting density of 63 weeks / 1.62 ㎡ in April 2012. On May 2, the amount of 15 ℓ / 1.62 ㎡ of N 1.5 ㎏/1,000 ㎡ was supplied to the non-treatment, liquid fertilizer of fermented rice bran, liquid fertilizer of fermented soybean, seaweed and liquid yeast. N 1.5 ㎏/1,000 ㎡ was put into the soil for the fermented soybean and leaf mold. The photosynthetic rate was measured on the middle part of the small leaf by using a photosynthetic analyzer (LI-6400, Li-COR) at 10 : 00 - 14 : 00 on a clear day under PAR (photosynthetically active radiation) of 100, 200, 400, 600, 800, 1,000, 1,400, 1,800 μmol s-1m-2. The growth features of ginseng (including the length of stem, length of leaf, width of leaf, length of root and yield, etc.) as well as leaf discoloration, and root rot, etc were examined. The statistical analysis was performed 3 times in the randomized block design. For the rate of photosynthesis, the liquid fertilizer of fermented rice bran > liquid fertilizer of fermented soybean ≒ untreated ≒ fermented soybean > seaweed > liquid yeast > leaf mold was higher in that order. The survival rate ranged from 42.9% to 51.9%. As for the above-ground growth characteristics, the liquid fertilizer of fermented rice bran, liquid fertilizer of fermented soybean, seaweed and liquid yeast showed higher performance than the non-treated while the fermented soybean and leaf mold showed similar performance to the non-treatment. As for the underground growth, the liquid fertilizer of fermented rice-bran, liquid fertilizer of fermented soybean, fermented soybean, or leaf mold showed the same performance as non-treatment while the fermented soybean or leaf mold showed shorter length than non-treatment. As for the yield, the liquid fertilizer of fermented rice-bran, or liquid fertilizer of fermented soybean showed better performance than non-treatment. Conclusion : In conclusion, it was found that liquid fertilizer of fermented rice bran and liquid fertilizer of fermented soybean are the suitable organic fertilizers which can be used in ginseng cultivation for improving the quantitative yield of 6-year-old ginseng in low-producing area.