벗초파리기생벌인 A. japonica의 벗초파리 유충의 발육단계에 따른 기생특성과 기생당한 유충과 번데기에서 형태적인 차이를 조사하였다. 또한 A. japonica의 우화기간이 벗초파리 우화일수 보다 더 소요되는 것을 확인하였다. A. japonica는 벗초파리 유충에 효과적인 기생 및 살충 효과를 나타내었으며 벗초파리 방제를 위한 천적으로 활용이 가능할 것으로 보인다.

This study was carried out to confirm the predatory and developmental features of N. stenoferus. We determine the host range of N. stenoferus. As a result, it was confirmed that Aphis gssypii, Myzus persicae, Planococcus citri, and Frankliniella occidentalis. N. stenoferus is thought to be able to feed on other micro pests. The test for a developmental period of N. stenoferus at 25℃ showed that the egg period was about 10 days. The nymphal period was about 18 days. Each nymphal period from 1st instar to 3rd instar nymphs was about 3 days. And the nymphal period of 4th and 5th were about 3.5 and 6 days, respectively. The female adult laid eggs in stem tissue or on leaves, and sometimes on the soaked cotton for water supply.

이리응애과의 많은 종들은 잎응애, 총채벌레 및 가루이와 같은 미소 해충의 생물학적 방제원으로 이용가능성이 높다. 실제로 칠레이리응애와 같은 포식활동이 왕성한 일부 종은 현재 농업에서 전세계적으로 많이 이용되고 있다. 특정 지역이나 생태계 또는 작물에 서식하는 이리응애과의 다양성 조사는 포식성 이리응애의 활용을 위한 좋은 기초자료가 된다. 그러나 국내에서 이와 관련된 자료는 90년대에 1건에 그쳐 자생 이리응애의 활용을 위한 후속 연구를 도모하기에 턱없이 부족한 실정이다. 본 연구에서는 중부지역의 6개도 17개 시군에서 2018년 7월부터 10월까지 자생 이리응애의 발생상황을 조사하였다. 그 결과 총 29과 57종의 식물종에 서식하는 20종의 이리응애를 확인하였다. 다양한 이리응애가 서식하는 식물종은 벚나무로 긴꼬리이리응애를 포함한 6종이 출현하였다. 서식 식물종이 가장 많은 이리응애는 긴꼬리이리응애로 17종이 식물에서 출현했고, 두 번째는 긴털이리응애로 16종의 식물에서 발견되었다.

This study was carried out to confirm the parasitic and developmental features of A. japonica and D. suzkii was used as a parasitic natural enemy. A. japonica attacked the D. suzukii larvae and the emergence of adults were observed from D. suzukii pupae. Black spots were observed in parasitized D. suzukii larvae. Mortality of parasitized larvae, rate of parasitic and developmental feature were investigated according to developmental stages of host, D. suzukii. Mortality and rate of parasitic of D. suzukii larvae were the highest when second instar larvae were attacked. Developmental period of parasitized D. suzukii larvae showed differences to developmental stages, but there was no significant difference in developmental stage of pupal period.

주로 열대와 아열대 지역에 분포하는 순모이리응애속(Euseius Wainstein) 응애류는 이리응애과(Phytoseiidae), 순이리응애아과(Amblyseiinae)에 속하며, 전세계적으로 193종이 보고되었다(Liao et al, 2017). 국내에는 순이리응애(E. finlandicus) 1종만이 기록되어 있다(Ryu, 2012). 잎응애류의 자생 천적자원 탐색조사 도중 본 속의 울릉도산 한국미기록종인 Euseius australis (Wu & Li, 1983)를 새롭게 확인하여 보고한다.

The Ministry of Environment in Korea published 'The Compilation of National List of Indigenous Insect Species ofthe Korean Peninsula' in 1996 including 11,853 species. The National Institute of Biological Resources (NIBR) originallycompiled the national species list of insects to establish a national inventory of endemic biological assets with the joinedefforts from all distinguished Korean taxonomists since 2008. For the convenience of taxonomists participating in theproject concurrently, the NIBR has developed the NIBR inventory description method and inventory system from 2008to 2017. Until September 2017 in this study, the NIBR has established the national insect inventory of 17,493 speciesincluding Collembola. The insect fauna of the Korean Peninsula comprises 579 families from 30 orders. The largest ordersare Coleoptera (114 families, 4,489 species), Lepidoptera (72 families, 3,868 species), Hymenoptera (63 families, 3,581species), Hemiptera (88 families, 2,102 species) and Diptera (79 families, 2,128 species). The fractions of the main insectorders in the fauna of the Korean peninsula correspond to those in the Holarctic temperate zone.

As a result of the curation of NIBR Insect Collection (NIBR, 2016), we found a new species and 4 newly recordedspecies, Tropideres distinguendus sp. nov., Acorynus latirostris (Sharp), Illis anna Shibata, Phaulimia confinis Sharp, Choraguscissoides Sharp. The genera, Acorynus Schoenherr and Illis Jordan are also newly recorded to Korean fauna. The newspecies, Tropideres distinguendus is easily distinguished from other species by the tubercles and dorsal pubescence pattern.Phaulimia confinis is similar to P. rufobasis, but easily distinguished from the latter by blackish elytral color and tibialpubescence band. Choragus cissoides is similar to C. cryphaloides, but easily distinguished from the latter by the absenceof lateral carina of pronotum.

The Ministry of Environment in Korea published 'The Compilation of National List of Indigenous Insect Species of the Korean Peninsula' in 1996 including 11,853 species. The National Institute of Biological Resources (NIBR) originally compiled the national species list of insects to establish a national inventory of endemic biological assets with the joined efforts from all distinguished Korean taxonomists since 2008. For the convenience of scores of professional taxonomic groups participating in the project concurrently, the NIBR has developed the NIBR inventory description method and inventory system from 2008 to 2016. For nine years in this study, the NIBR has established the national insect inventory of 16,558 species including Collembola. The insect fauna of the Korean Peninsula comprises 565 families from 30 orders. The largest orders are Coleoptera (110 families, 4,318 species), Lepidoptera (67 families, 3,733 species), Hymenoptera (61 families, 3,151 species), Hemiptera (88 families, 2,013 species) and Diptera (79 families, 1,944 species). The fractions of the main insect orders in the fauna of the Korean peninsula correspond to those in the Holarctic temperate zone. And totally 13 volumes of national list of insect species in Korea were published.

Anastatus orientalis Yang & Choi, 2015 (Hymenoptera: Eulpelmidae) is reported for the first time from Korea. It is a solitary endo-parasitoid in egg of Licorma delicatula (Hemiptera: Fulgoridae), Which causes damages to many ornamental and fruit trees in Korea. This species would be a good biological agent for suppression of the spotted lanternfly in Korea. More than 40 specimens of this species were collected in Cheonhyeon-dong, Hanam-city and Samsung-dong, Seoul city. This Korea species was compare with that from China in morphlogical characteristics and barcodes region of COI.

The genus of Cinara (Aphididae: Lachninae) live on the roots, trunk, branches, twigs, shoots or foliage of Coniferae and can be easily distinguished in having the very big body, apterae up to 5-6 ㎜ in length and short antennae which are shorter than half the body length. Most of species are monoecious without host alternation and attended by ants. Based on body appearance and COI gene sequence, we report Cinara cuneomaculata (Del Guercio) on Larix kaempferi, C. largirostris Zhang G. & W. Zhang on Pinus thunbergii and P. dnesiflora, C. smaragdina (Pashtshenko) on Abies holophylla and C. piceae on Juniperus rigida, for the first time in Korea.

Vespa crabro and V. analis are social hornet species commonly found in Asia, including Korea. Mastoparan is one of the major venom peptides of these two hornets but its amino acid sequence defers substantially. To examine the differences in the potential toxicity and bioactivity of mastoparans between these two social hornets, differential toxicological and pharmacological activities of synthesized mastoparan were investigated. V. analis mastoparan showed a 7-fold higher hemolytic activity, suggesting its higher cytotoxic potential compared with V. crabro mastoparan. Mastoparans from both hornet species exhibited similar levels of antimicrobial activities against Staphylococcus aureus and Botrytis cinerea, whereas the mastoparan from V. analis showed more potent antimicrobial activities against Escherichia coli and Candida albicans. Nevertheless, the antimicrobial activities of mastoparans of V. crabro and V. analis were relatively lower compared with those of other wasps. Both mastoparans also exhibited some levels of antitumor activity but the activity was significantly higher in V. analis mastoparan. In summary, the hemolytic, antimicrobial, and antitumor activities of synthesized V. analis mastoparan were higher than those of V. crabro mastoparan. These differential bioactivities are likely due to the amino acid sequence differences in the mature peptides. In particular, the additional Lys residue present in V. analis mastoparan may contribute to the higher levels of bioactivity as proposed by secondary structure prediction.

Vespa crabro is a cosmopolitan social wasp species whereas Vespa analis is commonly found in Asia. Both species are widely distributed in Korea and known to be aggressive when disturbed, resulting in frequent sting accidents. Although major venom components of well known Vespa wasps have been reported, no comparative transcriptomic analysis of venom gland between V. crabro and V. analis has been conducted to date. To investigate the differences in venom properties between these two wasps, total RNA was extracted from each venom gland and used for RNA-sequencing. A total of 31 venom-specific genes were identified in both venom gland transcriptomes but their expression profiles were different between V. crabro and V. analis. Venom allergen 5, premastoparan A and phospholipase A were the top three genes that were most prevalently transcribed in the venom gland of V. crabro, and their transcription rates were 902-, 112- and 4164-fold higher compared with V. analis, respectively, as judged by FPKM values. Their differential transcription profiles were confirmed by quantitative real-time PCR. In the venom gland of V. analis, however, premastoparan A was most abundantly transcribed gene, followed by calponin and tropomysin. In general, most venom-specific genes were more abundantly expressed in V. crabro but some genes exhibited higher transcription rates in V. analis, including muscle LIM protein, troponin, paramyosin, calponin, etc. Our findings suggest that V. crabro produce venom with much more enriched venom components, thereby with higher toxicity compared with V. analis.

Two species of the genus Asiacentistes (Hymenoptera: Braconidae) are reported in this study. The genus Asiacentistes is a small braconid group distributed in the East Palaearctic and Oriental regions, which has only two species in the world. One of them, Asiacentistes sinica Chen and Belokobylskij is new to Korea. Diagnosis and distribution in East Asia data are included.

Two species of the genus Cosmophorus (Hymenoptera: Braconidae) are reported in this study. Members of Cosmophorus are known as solitary koinobiont endoparasitoids of adults of barkboring and woodboring Scolytidae. The genus Cosmophorus of the subfamily Euphorinae is small in the size and enigmatic in the ecology, which has been recorded in Holarctic, Oriental, Afrotropical and Australian regions. Only five species are known in East Palaearctic. Among them, Cosmophorus (Cosmophorus) klugii Ratzeburg is new to Korea. Diagnosis and distribution data are included.

Two species of the subgenus Exosyntretus (Hymenoptera: Braconidae: Euphorinae) in the genus Syntretus are reported from Korea in this study. The species of the genus Syntretus are small parasitic wasps, which attack adult Hymenoptera. Their hosts are adult parasitoid wasps and adult bees. The subgenus Exosyntretus are known as only five species in the world. Among them, Syntretus (Exosyntretus) nevelskoii Belokobylskij is new to Korea. The world checklist, diagnosis and distribution data are included.