Background: This study explores the potential of discarded male layer embryos as a sustainable and non-GMO cell source for cultivated chicken meat production. The research aims to identify efficient methods for isolating muscle progenitor cells (MPCs) with high proliferative potential by conducting transcriptome analysis on thigh muscle tissues from both male and female chick embryos. Methods: Transcriptome analysis was performed on the thigh muscle tissues of male and female chick embryos, aged 12-13 days, (n = 4 each), to investigate the gene expression profiles and identify strategies for efficiently isolating MPCs. This approach aims to pinpoint techniques that would allow for the selection of MPCs with optimal growth and proliferation capabilities. Results: Using heatmap, hierarchical clustering, and multidimensional scaling (MDS), we found no significant sex-based differences in gene expression, except for the overexpression of the female-specific gene LIPBLL. The expression of muscle stem cell factors, including PAX3, PAX7, and other myogenic regulatory genes, showed no significant variation. However, to recover MPC-rich cells isolated from male thigh muscle, we found that by the pre-plating 7 stage, myogenesis-related genes, MYHs and MUSTN1 were minimally expressed, while the cell cycle arrest gene CDKN1A sharply increased. Conclusions: Our findings suggest that simple cell isolation directly from tissue is a more scalable and efficient approach for cultivated meat production, compared to labor-intensive pre-plating methods, making it a viable solution for sustainable research and resource recycling.

The purpose of the present study was to evaluate if main domestic vector mosquitoes, Aedes albopictus as a diurnal species and Culex pipiens as a nocturnal species, have correlation between reflecting luminous intensity of colors and preference for blood feeding activity. Laboratory evaluations were performed and the mosquito preferences were compared to three different color brightness such as light, primary, and dark for five fundamental color series including red, yellow, green, blue, and purple. The test bodies selected for these experiments were BioGents Sentinel traps (BG traps) with 20g dry ice in each falcon tube, which covered outside of the traps attached with three different luminous intensities such as light, primary, and dark for each different color of paper sheets. In each experiment, a pair of BG traps was placed 0.6 m away from each other in a 1.8 m x 3.7 m x 1.8 m screened enclosure. The enclosure maintained at 26±2℃, 65±5% RH, and 100 lux illumination. Evaluations were performed for two hours between 10:00 and 16:00 for each 25 Ae. ablopictus females and 15 hours from 18:00 to 9:00 for each 25 Cx. pipiens females. Each experiment was replicated four times. As a result, reflecting luminous intensity (L-value) affected and correlated negatively to the preference of the two mosquito species but the fundamental colors, green-red (a-value) and blue-yellow (b-value) were not correlated significantly to the preference (p=0.05). Aedes ablopictus females were preferred for feeding activity more to lower L-values of the colors and correlated negatively between color preference of the mosquitoes and the L-values such as r = -0.584 (p=0.003) for red, r = -0.606 (p=0.002) for yellow, r = -0.620 (p=0.001) for green, and r = -0.463 (p=0.023) for blue except purple (r = -0.129; p=0.549). Similarly, Cx. pipiens females were correlated negatively such as r = -0.590 (p=0.002) for red, r = -0.564 (p=0.005) for yellow, r = -0.687 (p=0.000) for green, r = -0.623 (p=0.001) for blue, and r = -0.689 (p=0.000). In conclusion, the color brightness were negatively correlated to the feeding activity of the two vector mosquito species but not the wavelength of visible ray. Also, the mosquito females preferred significantly darker ones in the same color series (p<0.05) except purple for Ae. ablopictus females.

This study conducted to predict the stock market prices based on the assumption that internet news articles might have an impact and effect on the rise and fall of stock market prices. The internet news articles were tested to evaluate the accuracy by comparing predicted values of the actual stock index and the forecasting models of the companies. This paper collected stock news from the internet, and analyzed and identified the relationship with the stock price index. Since the internet news contents consist mainly of unstructured texts, this study used text mining technique and multiple regression analysis technique to analyze news articles. A company H as a representative automobile manufacturing company was selected, and prediction models for the stock price index of company H was presented. Thus two prediction models for forecasting the upturn and decline of H stock index is derived and presented. Among the two prediction models, the error value of the prediction model ① is low, and so the prediction performance of the model ① is relatively better than that of the prediction model ②. As the further research, if the contents of this study are supplemented by real artificial intelligent investment decision system and applied to real investment, more practical research results will be able to be developed.

The fruit house fly Atherigona orientalis (Schiner), which is belonging to Muscidae, Diptera, is classified as quarantine pest and first reported in Korea at 2016. Annual occurrence and spatial distribution pattern of A. orientalis was biweekly monitored from April to December, using fly-wine trap in Korea. A. orientalis was first found in Gwangyang in June and increased populations by September, 2017. It is considering that A. orientalis is difficult to survive during winter season because this species is belonging to non-diapausing insect, so it may possible to be overwintering in warm place such as green house of paprika and tomato during winter. Based on this survey, A. orientalis is considered to be excluded from quarantine pests.

Plant quarantine is preventing the introduction and adaption of exotic pests in homeland, and then eventually promoting and improving domestic agriculture and forestry. Therefore, we conducted a survey of intercepted pests on hand-carried plants from all over the world to Korea. Totally 7,291 of hand-carried plants had been inspected by quarantine inspectors at 2017. Gimpo, Daegu, and Incheon International Airport showed more inspection cases than other areas by November 10, 2017, meanwhile, Gimhae and Incheon International Airport and Incheon Harbor showed more intercepting pest rate than other areas. In Animal and Plant Quarantine Agency, inspector found 490 cases of intercepted pests on 32 countries including China with 116 imported goods.

Grapholita molesta occur four times a year and Carposina sasakii occur twice a year, and both pests do damage on stone fruits such as peach, apple, plum, apricot, etc. Grapholita molesta is worldwide distributed in temperate and subtropical areas including South Korea. But, Carposina sasakii distributed in South Korea, Japan, China and Asia, and has been managed as an important import quarantine pest by the authorities of United States, Canada and Taiwan. Forecasting of both pests in Korea is currently done through the investigation of 1,000 fruits per 10 trees (100 fruits / tree) in designated peach orchard. However, this method is very difficult to observe the pest by bagging of peach and require too much time and labor. Therefore, we tried to carry out a new forecasting method by using of sex pheromone traps for newly standardized method as an alternative. Using sex pheromone trap, attractiveness of G. molesta was proved to be 2.5 > 1.5 > 0.5 m by the height and the border => outside > center by the position. Attractiveness of C. sasakii made no difference in height, but, more trapped at the center and border than outside in position at peach orchard.

This paper considers a scheduling problem in a two-machine flowshop with outsourcing strategy incorporated. The jobs can be either processed in the first machine or outsourced to outside subcontractors. This paper wants to determine which jobs to be processed in-house and which jobs to be outsourced. If any job is decided to be outsourced, then an additional outsourcing cost is charged The objective of this paper is to minimize the sum of scheduling cost and outsourcing cost under a budget constraint. At first this paper characterizes some solution properties, and then it derives solution procedure including DP (Dynamic Programming) and B&B (Branch-and-Bound) algorithms and a greedy-type heuristic. Finally the performance of the algorithms are evaluated with some numerical tests.

To profile the proteome in porcine plasma, blood samples were collected from adult male barrows and those plasma were retrieved. For the depletion or pre-fractionation of high-abundance proteins, plasma samples were treated with commercial kits. Then, protein profiling was initiated using one and two-dimensional electrophoresis. Proteins were spotted and then identified by MALDI-TOF-TOF and LC-MS-MS. In the results, more than forty six proteins were identified and the reference map was constructed. The pre-treatment for the removal of high-abundance proteins caused the changes in 2-DE images and some of the proteins were newly uncovered after the most of high abundant proteins were removed. However, it is expected for further steps necessary to identify more low-abundance proteins that may contain potential bio-markers.

Here, we present an approach of blood plasma proteome profiling and their comparisons between the young and the adult pigs as prerequisite for the identification of bio-markers related to the health conditions, growth performance and meat quality. To profile the proteome in porcine plasma, blood samples were collected from 19 young piglets and 20 adult male barrows and the plasma was retrieved. Then, protein profiling was initiated using one and two-di-mensional electrophoresis. Proteins were spotted and then identified by MALDI-TOF-TOF and LC-MS-MS. In the re-sults, more than thirty-six and twenty eight protein spots were selected in young piglets and adult pigs, respectively and twenty three proteins were identified. The proteome profile images were compared between those ones using Image Master Version 7.0. The image of expressed proteome showed that most of proteins from plasma of young pig-let separated clearly and concentrated in 2DE display compared to ones from adult. Image analysis in detail was car-ried out to look for the specific proteins related to age progression. It demonstrated that the characteristics of proteome expression could be distinct to their age stages. Further investigations needed to proceed to understand the age de-pendent change of protein conformation and biological meaning of those differences in proteome expression between young and mature adult pigs.

본 논문은 (1 : 1 : N) 재고모형에 대한 복수제품의 순환생산 및 배송 일정계획을 수립하는 연구를 수행하였다. 세부적으로 공급자가 원자재를 생산자에게 배송하면, 생산자는 순환생산방식을 활용하여 복수의 제품을 생산하여 N 구매자에게 배송하는 상황을 고려하고 있다. 본 연구의 목적은 공급자, 생산자, 구매자를 포함하는 시스템 전체의 비용을 최소화하는 계획을 수립하는 것이다. 최적해가 가지는 몇 가지 특성들을 분석하고, 이를 통해서 단계적인 휴리스틱 절

This paper considers a 2-stage assembly flowshop scheduling problem where each job is completed by assembling multiple components. The problem has the objective measure of minimizing total completion time. The problem is shown to be NP-complete in the str

This paper considers the simultaneously firing model for the artillery operations. The objective of this paper is to find the optimal fire sequence minimizing the final completion time of the firing missions of multiple artillery units for multiple target

This paper considers the simultaneously firing model for the artillery operations. The objective of this paper is to find the optimal fire sequence minimizing the final completion time of the firing missions of multiple artillery units for multiple targets. In the problem analysis, we derive several solution properties to reduce the solution space. Moreover, two lower bounds of objective are derived and tested along with the derived properties within a branch-and-bound scheme. Two efficient heuristic algorithms are also developed. The overall performances of the proposed branch-and-bound and heuristic algorithms are evaluated through various numerical experiments.

본 논문은 조립과 분리시스템이 혼합된 Fork-and-Join 시스템에서의 일정계획문제를 고려하고 있다. 최초 단계에서는 구성품 단위로 분리가 발생하고 두 번째 단계에서는 부품생산단계에서 각 부품 또는 구성품이 서로 다른 설비와 경로를 통해 독립적으로 생상된 후 최종 조립단계로 이동하게 되고, 그곳에서 조립공정을 통해 제품으로 안성된다. 본 논문에서는 이러한 Fork-and-Join 시스템에서 최종완료시간(makespan)을 최소화 할 수 있는 발견적

  This paper considers an integrated decision for scheduling and outsourcing(or, subcontracting) of a finite number of jobs(or, orders) in a time-sensitive make-to-order manufacturing environment. The jobs can be either processed in a parallel in-house fa

This study was carried out to investigate the effects of addition of aged garlic extract (AGE) on the color and lipid oxidation of ground chicken during refrigerated storage. The leg meat from 6 weeks-broiler was chopped and added with 4% of distilled water (control) and AGE, respectively, and then stored at 4±0.2℃ for 8 days. The commercial garlic was aged for the relatively short time (40-90℃/300 hr →natural dryness/40 hr→20-30℃/30-50 hr). The pH value was not significantly different between control and AGE-added chicken. The TBARS level of chicken was remarkably inhibited by addition of AGE during storage. In CIE color values, AGE-added chicken showed lower L* and higher b*, C* and Ho values than those of control during storage. Therefore, the addition of AGE in ground chicken enhanced the lipid oxidative stability and change to dark color.

개가시나무 가지의 에탄올 추출물에서 항산화활성을 관찰하였으며, 활성성분을 규명하기 위한 연구를 진행하였다. 그 결과, 4종류의 화합물을 분리하여 동정하였다. 분리 동정된 성분은 catechin(1), epi-catechin(2), tyrosol(3) 및 tiliroside(4)이다. 분리 성분의 항산화활성은 DPPH 라디칼 및 superoxide 음이온 라디칼 소거활성을 이용하여 측정하였다. 화합물 1, 2, 3, 4는 100 µL/mL 농도에서 각각 94.2 %, 93.4 %, 33.6 %, 11.2 %의 DPPH 라디칼 저해활성을 나타내었다. 또한, 화합물 1, 2, 3, 4는 200 µL/mL 농도에서 각각 60.2 %, 35.1 %, 20.6%, 4.5 %의 superoxide 음이온 라디칼 저해활성을 나타내었다. 화합물 1 ∼ 4는 개가시나무에서는 처음으로 분리된 물질이다.