Organoleptic parameters such as color, odor, and flavor influence consumer perception of drinking water quality. This study aims to evaluate the taste of the selected bottled and tap water samples using an electronic tongue (E-tongue) instead of a sensory test. Bottled and tap water's mineral components are related to the overall preference for water taste. Contrary to the sensory test, the potentiometric E-tongue method presented in this study distinguishes taste by measuring the mineral components in water, and the data obtained can be statistically analyzed. Eleven bottled water products from various brands and one tap water from I city in Korea were evaluated. The E-tongue data were statistically analyzed using multivariate statistical tools such as hierarchical clustering analysis (HCA), principal component analysis (PCA), and partial least squares discriminant analysis (PLS-DA). The results show that the E-tongue method can clearly distinguish taste discrimination in drinking water differing in water quality based on the ion-related water quality parameters. The water quality parameters that affect taste discrimination were found to be total dissolved solids (TDS), sodium (Na+), calcium (Ca2+), magnesium (Mg2+), sulfate (SO4 2-), chloride (Cl-), potassium (K+) and pH. The distance calculation of HCA was used to quantify the differences between 12 different types of drinking water. The proposed E-tongue method is a practical tool to quantitatively evaluate the differences between samples in water quality items related to the ionic components. It can be helpful in quality control of drinking water.

This study evaluated the effect of lactic acid bacteria (LAB, a mixture of Enterococcus faecium and Lactobacillus plantarum) supplementation, the storage temperature, and storage period on the fermentation characteristics and in vitro ruminal digestibility of a total mixed ration (TMR). The TMR was prepared into two groups, namely, CON (control TMR without the LAB) and ML (supplementing a mixture of E. faecium and L. plantarum in the ratio of 1% and 2% (v/w), respectively). Both groups were divided and stored at 4°C or 25°C for 3, 7, and 14 d fermentation periods. Supplementing LAB to the TMR did not affect the chemical composition of TMR except for the lactate and acetate concentration. Storage temperatures affected (p<0.05) the chemical composition of the TMR, including pH, lactate, and acetate contents. The chemical composition of TMR was also affected (p<0.05) by the storage period. During in vitro rumen fermentation study, the ML treatment showed lower (p<0.05) dry matter digestibility at 24 h incubation with a higher pH compared to the CON. There was no difference in the in vitro dry matter digestibility (IVDMD) of TMR between the CON and ML treatment however, at 24 h, ML treatment showed lower (p<0.05) IVDMD with a higher pH compared to the CON. The effects of storage temperature and period on IVDMD were not apparent at 24 h incubation. In an in vivo study using Holstein steers, supplementing LAB to the basal TMR for 60 d did not differ in the final body weight and average daily gain. Likewise, the fecal microbiota did not differ between CON and ML. However, the TMR used for the present study did include a commercial yeast in CON, whereas ML did not; therefore, results were, to some extent, compromised in examining the effect of LAB. In conclusion, storage temperature and period significantly affected the TMR quality, increasing acetate and lactate concentration. However, the actual effects of LAB supplementation were equivocal.

The present study was conducted to examine the effect of soybean silage as a crude protein supplement for corn silage in the diet of Hanwoo steers. The first experiment was conducted to evaluate the effect of replacing corn silage with soybean silage at different levels on rumen fermentation characteristics in vitro. Commercially-purchased corn silage was replaced with 0, 4, 8, or 12% of soybean silage. Half gram of the substrate was added to 50 mL of buffer and rumen fluid from Hanwoo cows, and then incubated at 39°C for 0, 3, 6, 12, 24, and 48 h. At 24 h, the pH of the control (corn silage only) was lower (p<0.05) than that of soybeansupplemented silages, and the pH numerically increased along with increasing proportions of soybean silage. Other rumen parameters, including gas production, ammonia nitrogen, and total volatile fatty acids, were variable. However, they tended to increase with increasing proportions of soybean silage. In the second experiment, 60 Hanwoo steers were allocated to one of three dietary treatments, namely, CON (concentrate with Italian ryegrass), CS (concentrate with corn silage), CS4% (concentrate with corn silage and 4% of soybean silage). Animals were offered experimental diets for 110 days during the growing period and then finished with typified beef diets that were commercially available to evaluate the effect of soybean silage on animal performance and meat quality. With the soybean silage, the weight gain and feed efficiency of the animal were more significant than those of the other treatments during the growing period (p<0.05). However, the dietary treatments had little effect on meat quality except for meat color. In conclusion, corn silage mixed with soybean silage even at a lower level provided a greater ruminal environment and animal performances, particularly with increased carcass weight and feed efficiency during growing period.

This study was conducted to evaluate the effect of lactic acid bacteria (LAB) inoculation to domestically-cultivated Italian ryegrass (IRG) on silage fermentation and in vitro ruminal fermentation. There were six treatments based on the LAB inoculants: 1) no addition of LAB (negative control: NC), additions of 2) commercially-available LAB (positive control: PC), 3) Lactobacillus plantarum (LPL), 4) L. paracasei (LPA), 5) L. acidophilus (LA), and 6) L. pentosus (LPT). All treatments were inoculated at a concentration of 106 CFU/g and ensiled for 3, 7, 21, and 42 days in triplicate and analyzed for nutritive values when ensiling was terminated. Day 42 silage from all treatments were also examined for in vitro ruminal fermentation. After 42 days, LAB-inoculated silages had higher (P<0.05) lactic acid concentration compared to the NC. In terms of nutritive values, the silages treated with LPA, LA, and LPT showed higher (P<0.05) crude protein and lower (P<0.05) neutral detergent fiber and acid detergent fiber content compared to the rest of the treatment. In vitro ruminal dry matter degradability was not affected by LAB addition. However, LAB-treated IRG had shown higher (P<0.05) ammonia-N compared with that of the NC. LPA had shown the highest (P<0.05) volatile fatty acid concentration among the LAB examined. In conclusion, the addition of a single strain of LAB appeared to produce a quality IRG silage compared with the NC and the PC. Among the strains examined, LPA seemed to be superior to the others.

본 논문은 다국적 기업 해외 R&D 자회사의 지식 습득 방향에 있어서 자회사의 글로벌 조직 정체성(global organizational identity)이 미칠 수 있는 영향에 대하여 탐색하였다. 사회 정체성 이론에 근거하여, 본 연구에서는 다국적 기업 해외 R&D 자회사가 본국도 현지도 아닌 그 외의 국가에서 지식을 습득하는 글로벌 지식 습득 행위를 하는데 있어서 해외 R&D 자회사의 글로벌 조직 정체성이 미칠 수 있는 영향에 대해 탐구하였다. 자아 존중감을 향상시키고 불확실성을 감소시키기 위한 목적에서 다국적 기업 해외 R&D 자회사는 자신의 조직 정체성을 일치시키는 방향으로 자신의 태도와 행위를 적합시키게 된다. 사회 정체성 이론에 기초하여, 본 연구에서는 해외 R&D 자회사가 글로벌 혁신가로서 명확한 사명을 가질 때, 보다 높은 자율성을 가질 때, 그리고 글로벌 파트너와의 관계에 보다 깊이 배태되어 있을 때 글로벌 지식 습득 행위를 보다 활발하게 할 수 있을 것이라고 예측 하였다. 그리고 해외 R&D 자회사의 기술적인 역량이 글로벌 조직 정체성 관련 요인들과 글로벌 지식 습득 간의 관계를 강화하는 조절 효과에 관한 가설 또한 수립하였다. 가설 검증을 위해 본 연구에서는 일본 다국적 기업의 해외 R&D 연구소들의 설문 자료와 미국 특허청의 특허 인용 자료를 결합하여 음이항 회귀 모형으로 분석하였다. 실증 분석 결과 해외 R&D 자회사의 자율성과 글로벌 관계에의 배태 정도가 글로벌 지식 습득에 긍정적 영향을 미치는 것으로 나타났으며, 해외 R&D 자회사의 자율성과 글로벌 지식 습득 간의 관계를 자회사의 기술적 역량이 강화하는 것으로 나타났다.

본 연구는 다국적 기업의 해외 R&D 자회사가 본사로부터 지식을 소싱하는 정도에 자회사의 기술 역량과 자회 사와 본사간의 관계 배태성이 미치는 영향을 탐색하였다. 이를 위해 글로벌 반도체, 통신장비, 제약 산업에 속한 34개 다국적 기업의 86개의 해외 R&D 자회사가 미국 특허청에 출원한 특허데이터를 분석하였다. 실증분석 결 과, 해외 R&D 자회사는 절대적 수준의 기술 역량이 높을수록 본사로부터 지식을 더 많이 소싱하는 반면, 자회 사의 본사 대비 상대적 수준의 기술 역량이 높을수록 본사로부터 지식을 덜 소싱하는 것으로 나타났다. 또한, 해 외 R&D 자회사와 본사간의 기술적 배태성과 사회적 배태성은 자회사가 본사로부터 지식을 소싱하는 정도에 모 두 긍정적인 영향을 미치는 것으로 나타났다.

Pesticide application in agriculture provides significant benefits such as protection from disease, prevention of harmful insects, and increased crop yields. However, accurate toxicological tests and risk assessments are necessary because of many related adverse effects associated with pesticide use. In this review, we discuss and analyze residual pesticides contained in livestock feed in Korea. A pesticide residue tolerance standard for livestock feed has not been precisely established; so, risk assessments are required to ensure safety. Standards and approaches for animal criteria and appropriate methods for evaluating residual pesticides are discussed and analyzed based on technology related to animal product safety in Korea. The safety of livestock feed containing pesticides is assessed to establish maximum residue limits relative to pesticides. Analysis of residual pesticides in milk, muscle, brain, and fat was performed with a livestock residue test and safety evaluation of the detected pesticide was performed. Efficacy of organic solvent extraction and clean-up of feed was verified, and suitability of the instrument was examined to establish if they are effective, rapid, and safe. This review discussed extensively how pesticide residue tolerance in livestock feed and hazard evaluation may be applied in future studies.

This study was conducted to examine the effect of corn (Zea mays L.) - soybean (Glycine max L.) silage prepared by intercropping method on the nutritive value of the silage, in vitro rumen fermentation characteristics, dry matter degradability, as well as milk yield and milk composition of dairy cows. In a couple of experiments intercropped corn-soybean silage (CSBS) was compared with corn silage (CS) and/or Italian ryegrass hay (IRG). Numerically, CSBS had higher crude protein, ether extract, and lactic acid contents compared to CS. In vitro rumen fermentation analysis demonstrated that up to a 24-h incubation period, both CS and CSBS showed higher total gas production, ammonia N concentration, and dry matter degradability compared to IRG (p<0.05). The investigation on animals was conducted in a commercial dairy farm located in Gyeongju, South Korea, employing 42 Holstein cows that were divided into 2 group treatments: CS and CSBS in a completely randomized design. Although no significant difference was observed in milk yield, animals fed on CSBS showed significantly higher milk protein (p<0.05) and milk fat content (p<0.01), compared to animals fed on CS. Taken together, our findings indicate that corn-soybean silage that is cultivated, harvested, and prepared through intercropping can improve the protein content of the silage, and can also enhance in vitro rumen fermentation, dry matter degradability, and performance of dairy cattle.

본 연구는 일본 다국적 기업의 해외 연구소에 관한 실증 연구를 통하여 다국적 기업 해외 R&D 자회사의 조합 역량, 습득 역량, 자율성이 자회사의 혁신 성과에 미치는 영향에 관하여 검증하는 논문이다. 본 논문에서는 혁신 의 성과에 영향을 미칠 수 있는 요인으로 다양한 지식의 원천에서 어떻게 지식을 골고루 습득하고 조합하여 활용 하는지가 중요하다는 점에 주목하였다. 본 연구에서는 해외 자회사의 혁신 역량의 원천을 습득 역량(sourcing capability)과 조합 역량(combinative capability)으로 구분한 후 이 역량들이 혁신 성과에 미치는 영향을 심 층 분석하였다. 특히 조합 역량을 본국과 현지, 그 외의 국가의 지식들을 조합하는 역량과 다국적 기업 내부의 지식과 외부의 지식을 조합하는 역량으로 세분하고 각각의 조합 역량이 혁신 성과에 미치는 영향에 관한 가설을 수립하였다. 또한 조합 역량이 습득 역량과 혁신 성과 간의 관계를 강화할 수 있는지와 자율성이 조합 역량과 혁 신 성과 간의 관계를 강화할 수 있는지를 검증해 보았다. 일본 다국적 기업의 해외 연구소에 관한 설문 자료와 특허 자료를 결합하여 실행한 음이항 회귀 분석 결과 해외 자회사의 본국-현지-그 외의 국가의 지식을 조합하는 역량과 습득 역량, 자율성이 해외 자회사의 혁신 성과에 긍정적인 영향을 미치는 것으로 나타났다. 그리고 본국- 현지-그 외 국가의 지식을 조합하는 역량이 해외 자회사의 습득 역량이 혁신 성과에 미치는 긍정적 영향을 강화 하는 것으로 나타나 습득 역량과 조합 역량은 상호 보완적인 역할을 하는 것으로 나타났다.

This study was conducted to investigate the antibacterial, antioxidant, and in vitro greenhouse gas mitigation activities of fermented Scutellaria baicalensis Georgi extract. Seven starter cultures were used, comprising four of lactic acid bacteria and three of Saccharomyces cerevisiae. Ten grams of S. baicalensis Georgi powder was diluted in 90 mL autoclaved MRS broth. Each seed culture was inoculated with 3-10% (v/v) S. baicalensis Georgi MRS broth and incubated at 30℃ for 48 h. Among the starter cultures used, only Lactobacillus plantarum EJ43 could withstand the fermentation conditions. This fermentation broth was dried and extracted with ethanol to assess its antibacterial, antioxidant, and in vitro methane mitigation activities. The extract of S. baicalensis Georgi fermented by L. plantarum EJ43 (SBLp) showed higher antibacterial activity (bigger clear zone) compared to the unfermented S. baicalensis Georgi extract (SB0). SBLp also presented 1.2 folds higher antioxidant activity than SB0. During in vitro rumen fermentation, SBLp showed reduction in methane production compared to SB0 or the control. In conclusion, fermentation by L. plantarum EJ43 may enhance antibacterial and antioxidant activities of S. baicalensis Georgi and decrease enteric methane production.