본 연구는 냉동 오징어튀김 제품을 단체급식에서 대량튀김 조리하면서 대두유에서 발생하는 휘발성 알데히드류를 측정함으로써 대량 조리가 진행됨에 따라 발생하는 대두유의 품질저하를 평가하고자 하였다. 휘발성 알데히드류는solid phase micro-extraction(SPME)과 gas chromatography-mass spectrometry(GC-MS)을 이용하여 분석되었으며pentanal, hexanal, heptanal, (E)-2-heptenal, octanal, (E,E)-2,4-heptadienal, (E)-2-dodecenal, nonanal, (E)-2-nonenal,decanal, (E,E)-2,4-nonadienal, 4-oxononanal, 2-decenal,undecanal, (E,E)-2,4-decadienal, 2-undecenal 등 총 16가지 성분들이 검출되었다. 대조군인 미사용 대두유에서는 9가지성분들만이 소량 검출되었으며, pentanal, hexanal, (Z) or(E)-2-heptenal, (E,E)-2,4-heptadienal, (E,E)-2,4-nonadienal 등5가지 알데히드는 튀김사용 횟수 3회 이상에서 유의적으로증가하였고, (E)-2-dodecenal, nananal, (E)-2-nonenal, 4-oxononanal, (Z) or (E)-2-decenal, (E,E)-2,4-decadienal, 2-undecenal 등 7가지 알데히드는 대조군에 비해 튀김사용횟수 1회에서부터 유의적 차이를 보였으나 그 후 튀김횟수 증가에 따른 유의적 증가는 없었다.
The effect of electrochemical surface treatments in KOH chemical solution on microstructures of carbon blacks was investigated in terms of surface functional values and XRD measurements. And their mechanical interfacial properties of the carbon blacks/rubber composites were studied by the composite tearing energy (GIIIC). It was found that the development of basic-surface functional groups lead to the significant physical changes of carbon blacks, such as, decrease of the interlayer spacing (d002), increase of the crystalline size along c-axis (Lc), and increase of degree of crystalline (χc). This treatment is possibly suitable for carbon blacks to be incorporated in a hydrocarbon rubber matrix, resulting in improving the hardness and tearing energy of the resulting composites.
The objective of this study was to determine the genetic diversities of major rice blast resistance genes among 84 accessions of aromatic rice germplasm. Eighty four accessions were characterized by a dominant 11 set of PCR-based SNP and CAPS marker, which showed the broad spectrum resistance and closest linkage to seven major rice blast resistance (R) genes, Pia, Pib, Pii, Pi5 (Pi3), Pita (Pita-2), and Pi9 (t). The allele specific PCR markers assay genotype of SCAR and STS markers was applied to estimate the presence or absence of PCR amplicons detected with a pair of PCR markers. One indica accession, Basmati (IT211194), showed the positive amplicons of five major rice blast resistance genes, Pia, Pi5 (Pi3), Pib, Pi-ta (Pi-ta2), and Pik-5 (Pish). Among 48 accessions of the PCR amplicons detected with yca72 marker, only five accessions were identified to Pia gene on chromosome 11. The Pib gene was estimated with the NSb marker and was detected in 65 of 84 accessions. This study showed that nine of 84 accessions contained the Pii gene and owned Pi5 (Pi3) in 42 of 84 accessions by JJ817 and JJ113-T markers, which is coclosest with Pii on chromosome 9. Only six accessions were detected two alleles of the Pita or Pita-2 genes. Three of accessions were identified as the Pi9 (t) gene locus.
Brown planthopper (BPH) is a serious insect pest of rice crop throughout rice growing countries, and yield loss due to its infection can be up to 60%. This study aimed to evaluate efficiency of molecular markers for screening BPH resistance accessions among 86 aromatic rice germplasm Eighty-six accessions of aromatic rice germplasm included two accessions of Tongil type (bred in Korea), 28 accessions of japonica type and 56 accessions of indica type. We applied eight STS markers (pBPH9, pBPH19, pBPH20, pBPH21, AJ09-b, RG457L, RG457B, and 7312.T4A) which were linked to four of BPH resistance genes, Bph1, Bph13(t), Bph10, and Bph18(t) respectively. One japonica type accession, 415XIr352, and six indica type accessions possessed one or four positive bands when tested with four STS markers linked to Bph1 gene. One indica type aromatic rice, Basmati9-93, showed the target bands linked to the Bph10 gene. The other accessions did not show same fragments as the respective resistant lines. Bph13(t) is the most widely introduced resistance gene and only one accession showed positive bands implying that this accession might harbor Bph10 and Bph18(t) genes. Three aromatic accessions, Domsiah, Khao Dawk Mali 105 and 415XIr352 showed gene pyramiding of Bph1 and Bph13(t). Two indica aromatic rice, Ds 20 and Basmati 9-93, possessed at least two BPH resistance genes, Bph1, Bph18(t) and Bph13(t), Bph18(t), respectively. These results indicates that aromatic rice germplasm have narrow diversities of BPR resistance genes.
More than 2,500 accessions of squash germplasm are preserved in the National Agrobidiversity Center of RDA, Korea. We investigated the ecological and morphological characteristics on 100 accessions of the squash germplasms with a low viability. Two typical types of oriental squash (Cucurbita moschata) and western squash (Cucurbita maxima) are possessed 89 and 11 accessions, respectively. The origin of 100 accessions was divided into three groups, group I (Korea landraces with 81 accessions), group II (Nepal with 15 accessions) and group III (Russia, Uzbekistan, Laos, and Mexico with each one accession). Seventy eight accessions germinated at 27~circC in growth chamber. Days to first flowering of the female and male flower ranged from 32 days to 67 days and 24 days to 55 days after planting, respectively. Vine length showed the largest variation ranging from 357.8 cm to 1,465 cm, also the average length of ten stem node range has a variable ranges from 92 cm to 217.5 cm. All regenerated squash accessions have an only collided tendril and have three to seven lateral shoots. The flattened globe-shaped squash is the most predominant and exhibited ordinarily round shaped, ball shaped, long oblong-shaped, elongated shape, and pear shape. The average weight of fruit ranged from 1.33 kg to 7.95 kg. Sugar contents showed 5.4~12.6 Brix~circ without ripening period.
The volatile and semi-volatile compounds of 5 accessions of domestic and 25 accessions of foreign aroma rice were extracted by solid phase microextraction (SPME) and analyzed by gas chromatography-mass spectrum (GC-MS). A total of 158 volatile and semi-volatile compounds were identified from 30 accessions of aroma rice germplasm, including 32 alcohols, 25 acids, 25 ketones, 21 hydrocarbon, 18 esters, 16 aldehydes, 4 ethers, 5 amines, 2 phenols, 2 bases, and 8 miscellaneous compounds. By UPGMA/Neighbor-join tree analysis, the thirty aroma rice germplasms could be classified into five groups according to the major odor or aroma compounds. Group Ⅰ included Indica type of 'Basmati' varieties. GroupⅡ included Japonica type foreign aroma rice except WAR 16 (domestic aroma rice). GroupⅢ and GroupⅤ included Indica type of Basmati and non-Basmati varities. GroupⅣ included domestic aroma rice except WAR34 and WAR35(Basmati varieties).
This study was conducted to provide the fundamental data based on the agronomic, phsyco-chemical characteristics, and quntification of volatile compounds of aroma rice germplasms. Among the 104 rice germplasm, five accessions of domestic aroma rice, 14 accessions of widely cultivated rice, and 60 accessions of foreign aroma rice were selected with excellent agronomic traits, high fertility and germination rate. The average amylose content of foreign aroma rice was higher than domestic aroma rice and widely cultured domestic rice. The domestic aroma rice was shown low protein and the foreign Indica types of aroma rice exhibited a wide range of protein contents. The foreign aroma rice were distributed a variable range of ADV value and lower than the both domestic aroma rice and widely cultured domestic rice. And it shown the difference aspect of amylograms according to the eco-type of the domestic and foreign aroma and the general rice, and it was distinguishable in difference of the aspect of the endosperm of rice as non-glutinous rice, waxy rice, and middle-waxy rice. The optimal extraction conditions of 2-Actyl-1- Pyrroline is recommended that five second grinding time, using 100 % ethyl alcohol, at 90℃ for 30 minutes. The domestic and the foreign aroma rice has more than mild aroma grade. A total 30 accession of aroma rice germplasm selected based on their 2AP concentration, aroma grade and agronomic traits. A total of 158 volatile and semi-volatile compounds were identified from 30 accessions of aroma rice germplasm, including 32 alcohols, 25 acids, 25 ketones, 21 hydrocarbon, 18 esters, 16 aldehydes, 4 ethers, 5 amines, 2 phenols, 2 bases, and 8 miscellaneous compounds. The thirty aroma rice germplasms could be classified into five groups according to the major odor or aroma compounds.