목적 : 천연 항균, 항산화 물질인 망고스틴의 크산톤 화합물로 코팅된 콘택트렌즈를 제조하여 안과적 질환 예 방을 위한 기능성 콘택트렌즈의 물리·화학적 특성을 연구하였다. 방법 : 콘택트렌즈를 제조하여 IPN기술을 통해 감마망고스틴을 코팅하였다. 제조된 렌즈를 ISO와 식약처기준 을 참고하여 광투과율, 함수율, 산소투과율(Dk/t), 항균, 항산화성 실험을 진행하였다. 결과 : 감마망고스틴 코팅 콘택트렌즈의 가시광선투과율은 93%이다. 자외선은 70% 이상 청광은 30% 이상 차 단한다. 항균 실험에서는 감마망고스틴 코팅 콘택트렌즈의 항균성이 일반렌즈에 비해 5배 이상 효과 있고, 항산화 는 시험별 17.49, 28.46, 36.99%의 항산화율을 보였다. 결론 : 감마망고스틴으로 코팅된 콘택트렌즈는 UV와 청광의 차단율이 일반렌즈에 비해 매우 뛰어나다. 또한, 일반렌즈에 없는 항균, 항산화 활성을 보여 안과적 질환을 예방할 수 있을 것으로 보인다.

본 연구에서는 채소정식을 위한 정식기에 사용하는 생분해성 포트를 개발하기 위하여 생분해성 첨가제의 비율에 따라 포트의 물성 및 식물의 생장 차이를 구명하였다. 본 실험에 사용된 생분해포트의 주원료는 크라프트지와 신문고지였고, 생분해성 포트는 주 배합비에서 내첨첨가제의 함량을 주원료 대비 각 3%, 5%로 제조하였다. 본 실험에서 8주 육묘 후 포트의 물리적 특성과 첨가제에 따른 변화를 알아보기 위해 포트의 인장강도, 두께, 무게 등을 조사하였다. 생분해성 첨가제가 함유된 포트와 일반 PE포트에 식물 생장도 비교하였다. 2주차에서 5주차에는 매주 배추의 생육조사를 진행했고, 5주차에서 8주차에는 고추생육조사를 진행하였다. 식물의 생장은 뿌리신선중(g), 지상부 시선중(g), 옆 장(cm), 옆 폭(cm)등을 측정하였다. 생분해성 포트에서의 식물 생장은 플라스틱 포트에 비해 생육이 저조하게 나타났다. 생분해성 포트의 무게와 두께는 첨가제 함량에 따라 낮은 상관성을 보였지만, 인장강도의 경우 차이를 보여 내첨제의 비율에 따라 생육에 영향을 미치는 것으로 나타났다. 그러나 첨가제는 무게와 두께에는 영향을 미치지 않아 포트의 생분해 능력에는 영향이 없는 것으로 판단된다. 본 연구는 생분해성 식물 포트 개발의 기초자료가 될 것으로 기대된다.

본 논문에서는 상용코드인 ANSYS CFX를 통한 해양레저 스포츠 및 야외 활동 시 사용 가능한 휴대용 수평축 수차의 유입유속(U) 및 주속비(TSR, Tip Speed Ratio) 변화에 따른 성능해석을 수행하였으며, 해석결과 및 유동장 분석을 통해 설계에 대한 검토 및 장치의 성능을 확인하였다. 또한, 추가적으로 블레이드의 피치각도(αpitch) 변화에 따른 성능해석을 통해 수차의 성능개선에 필요한 데이터를 획득하고자 하였다. 본 논문의 연구 결과 수치해석 케이스 중 주속비 4인 경우, 모든 유입속도 및 블레이드 피치 각도에서 가장 높은 성능을 보였으며, 설계 유속 이하의 일부 조건에서도 설계 출력인 30 W 이상의 출력을 보였다. 그리고 수치해석 케이스 중 가장 높은 출력과 출력계수는 유입유속 1.5 m/s, 블레이드 피치 각도 3°, 주속비 4에서 보였으며, 출력 약 85 W, 출력계수 약 0.30이었다.

This study investigated the synergistic effect of single inoculation and co-inoculation of phosphate-solubilizing bacteria (PSB) Burkholderia metallica JH-7 and Burkholderia contaminans JH-15. Phosphate-solubilizing abilities of these strains were assessed by measuring phosphorus content in culture media that were singly inoculated or co-inoculated with these strains for 7 days. B. metallica JH-7 was found to release the highest content of soluble phosphorus (140.80 μg mL-1) into the medium, followed by single inoculation of B. contaminans JH-15 (135.95 μg mL-1) and co-inoculation of two strains (134.84 μg mL-1). The highest pH reduction, organic acid production, and glucose consumption were observed in the medium inoculated with B. metallica JH-7 alone compared with that in the medium co-inoculated with both the strains. Results of a plant growth promotion bioassay showed 17.4% and 7.48% higher leaf and root growth, respectively, in romaine lettuce inoculated with B. metallica JH-7 alone than in romaine lettuce inoculated with a control strain. However, no significant difference was observed between single inoculation and co-inoculation of these strains with respect to phosphorus release and plant growth. Although the results of the present study did not show the synergistic effect of phosphate solubilization by the PSB strains examined, these results indicate that treatment with PSB exerts a beneficial effect on crop growth.

In the present study, the effect of cysteine and NT or bisphenol A (BP) on in vitro aturation (IVM) of porcine oocytes were examined. COCs was cultured in NCSU-23 medium supplement with 10% FCS which had previously been covered with mineral oil and equilibrated in a humidified atmosphere of 5% CO2 and 95% air at 38℃. The IVM rate of oocytes cultured for 48 hrs in NCSU-23 medium supplement with 0.5～10.0 mM cysteine were 34.0±3.2%, 36.0±3.5%, 48.0±3.8%, 22.0±3.2%, respectively. The IVM rate of oocytes cultured in NCSU-23 medium supplement with 0.5～5.0 mM NT for 48 hrs were 24.0±4.2%, 18.0±4.9%, 8.0±2.2%, respectively. NT affects oocyte in vitro maturation rate in a dose-dependent. This result were significantly lower than the control group. The IVM rate of oocytes cultured for 48 hrs in NCSU-23 medium supplement with 1.0 mM NT+5.0 mM cysteine (38.0±4.3%) were significantly higher than that of NT treatment. The IVM rate of oocytes cultured in NCSU-23 medium supplement with 0.05～5.0 mM BP for 48 hrs were 20.0±4.7%, 10.0±5.3%, 6.0±3.2%, respectively. The IVM rate of oocytes cultured in NCSU-23 medium supplement with BP was significantly lower cultured non supplement of BP (44.0±3.5%). BP affects porcine oocyte maturation rate in a dose-dependent manner. The IVM rate of oocytes cultured for 48 hrs in NCSU-23 medium supplement with 1.0 mM BP+5.0 mM cysteine (32.0±3.2%) were increased than that of BP treatment.

These study was carried out to investigate the effects of the supplementation with sodium nitroprusside (SN) and nitric oxide (NO) of canine oocytes on IVM rates. Oocytes were incubated in TCM-199 supplement with at 0.03～0.10 mM SN and 0.3～1.0 mM NO for 48 hrs. Oocytes were transferred to 50 ul drops of maturation medium covered mineral oil and cultured in a CO2 incubator (5% CO2, 95% air, 38℃). The in vitro maturation rate of oocytes cultured for 48 hrs in TCM-199 medium supplement with 0.03, 0.05, 0.07, 0.10 mM SN were 25.9±3.5%, 36.4±3.2%, 33.3±3.5%, 28.8±3.2%, respectively. The in vitro maturation rate of oocytes cultured for 48 hrs in TCM-199 medium supplement with 0.03～0.07 mM SN were significantly increased compare to the control (26.0±2.2%). The in vitro maturation rates of oocytes cultured for 48 hrs in TCM-199 medium supplement with 0.3, 0.5, 0.7, 1.0 mM NO were 28.0±4.2%, 36.5± 3.6%, 30.0±3.8%, 19.2±3.5%, respectively. The in vitro maturation rate of oocytes in TCM-199 medium supplemented with 0.3 and 0.5 mM NO were significantly increased compare to the control (26.0±2.2%). The in vitro maturation rates of oocytes cultured for 12～48 hrs in TCM-199 medium supplement with 0.05 mM SN were 26.0±3.2%, 28.0±3.4%, 38.0±3.2%, respectively. The in vitro maturation rate of oocytes cultured for 12～48 hrs in TCM-199 medium supplement with 0.5 mM NO were 22.0±3.0%, 30.0±3.8%, 36.0±4.2%, respectively. These result was significantly increased compare to the control.

These study was to investigate the in vitro fertilization and viability of fresh and vitrified oocytes. Also, the developmental capacity of IVF and intracytoplasmic sperm injection (ICSI) oocytes were investigated. Then vitrification was performed with the use of 20% ethylene glycol + 20% DMSO + 0.5 M sucrose + 10% FCS + TCM-199 medium. Vitrification immature oocytes are cultured in vitrification solution for 10 min afterwards transferred to expose at room temperature for 5 min. and transferred to the ice water for 5 min. The oocytes were sealed in a 1.0 mm straw and placed in a LN2 container. Frozen oocytes were rapidly thawed in a water bath at 30～35℃, and then placed in TCM-199 medium containing 0.5 M sucrose for 5 min each, respectively, at 38℃. After being washed for 2～3 times, using fresh medium the oocytes were cultured in TCM-199 medium supplemented with 5% FCS at 38℃ in 5% CO2 and air. The normal morphology of fresh and vitrified-thawed oocytes were 87.1±2.1% and 54.8±2.5%, respectively. The viability rates of fresh and vitrified-thawed oocytes were 70.0±2.2% and 41.9±2.6%, respectively. Viability rates of vitrified-thawed oocytes were lower than that of fresh follicular oocytes (p<0.05). The in vitro maturation rates of fresh and vitrified oocytes were 45.1±3.6% and 28.9±4.4%, respectively. The IVF rates of fresh follicular and vitrified-thawed oocytes were 34.0±2.2% and 20.2±2.6%, respectively. The in vitro maturation and fertilization rates of vitrified-thawed oocytes were lower than those of the fresh follicular oocytes (p<0.05). A total of 350 oocytes were fixed and stained after co-incubation with spermatozoa, of which 88 had identifiable nuclear material. After IVF for 20 hrs, 25.1±3.4% of the oocytes found to have been penetrated by spermatozoas. Oocytes were fixed and stained after ICSI, and 105 oocytes contained identifiable nuclear material. After IVF and ICSI for 20 hrs, 34.3±3.4% and 59.0±2.0% of the oocytes were found to have been penetrated by spermatozoas. The developmental rates upon ICSI were significantly higher than those of the IVF method (p<0.05).

The in vitro maturation rate of vitrified-thawed canine oocytes was 30.8±3.4%. The in vitro maturation rate of vitrified oocytes was lower than that of the control (52.0±2.5%, p<0.05). The in vitro maturation rate of vitrified-thawed oocytes were significantly (p<0.05) lower than those of fresh oocytes. The in vitro maturation and developmental rates of the vitrified-thawed oocytes were 17.5±2.5% and 8.8±3.4%, respectively. This results were lower than the control group (43.6±3.2% vs 20.0±3.0%). SOD1 gene expression of 1～2 mm of follilce size were higher than those of above 6 mm follicle size. SOD2 gene expression of 1～2 mm of follicle size were significantly higher than those of above 6 mm follicle size (p<0.01). The expression pattern of SOD1, 2 was constantly expressed in both groups but strongly expressed in follicles (1～2 mm) group when compared to the above 6 mm follicles. SOD gene expression between groups the fresh and vitrified oocytes groups were significant differences in rates. However, RGS gene expression between groups the fresh and vitrified oocytes groups were no significant differences in rates.