Recruitment of primordial follicles(PMF) is crucial for female fertility. however, factors and mechanisms that regulate this process is poorly understood. The present study was conducted to obtain an inclusive view of the gene expression and to identify novel factors and their pathways of regulating PMF arrest and/or growth initiation. Ovaries from one-day neonatal(consists of oocyte and PMF) and five-day old(consists of PMF and primary follicles, PRIF) mice were collected, either total RNA or mRNA was isolated, and suppression subtractive hybridization(SSH) was used to isolate and clone genes that differentially expressed in day 1 and day 5 ovaries. Confirmation that some of these genes are differentially expressed in PMF and/or in PRIF was accomplished by using laser captured microdissection(LCM), RT-PCR. in situ hybridization(ISH) and/or immunohistochemistry(IHC). In toto, 357 clones were sequenced and analyzed by BLAST and RIKEN program. Sequences of 330 clones significantly matched database entries while 27 clones were novel. Forty-two and 47 different genes were identified as differentially expressed in day 1 and day 5 ovaries, respectively, while 7 genes were expressed in both stages of ovaries. Day 5-subtracted library included several genes known as markers far growing follicles, such as ZP2, MATER, and fetuin. Among the genes with assigned functions, 23.8% was associated with cell cycle/apoptosis regulation, 7.1% with cellular structure, 11.9% with metabolism, 26.2% with signal transduction, and 31.0% with gene/protein expression in day 1; while 10.6%, 17.0%, 23.5%, 25.5%, and 23.4% in day 5, respectively. Genes such as GDF-8, Lats2, Septin2, and Weel were the highly expressed genes in PMF, while HSP84, Laminin2, MATER, MTi7, PTP, and Wrn were highly expressed genes in PRIF. We have successfully discovered list of genes expressed in day 1 and day 5 ovaries and confirmed that some of them are differentially expressed in PMF and/or PRIF. Gene expression profile from the present study would provide insight for the future study on the mechanism(s) involved in primordial-primary follicular transition. This work was Supported by Korean Health 21 RND Project, Ministry of Health and Welfare, Korea (01-PJ10-PG6-01GN13-0002).

Most apartment houses have been constructed with concrete structures. However, underground parking lots and rooftops in apartment buildings frequently have cracks due to drying shrinkage and various degradation factors. The leakage caused by this cracks has a problem that the durability of the structure and the waterproof performance is deteriorated. Therefore, the development of a proper crack repairing materials is continuously required. The purpose of this study is to apply chemically bonded ceramics (CBC) as a repair material to repair cracks in concrete, which has strong adhesion, durability, rapid hardening performance. Therefore, it was applied to the roof crack of the apartment(mock-up test) and the performance was evaluated. As a result, the compressive strength and the bond strength showed excellent performance. In the case of the dry shrinkage characteristics, the performance was stable in the range of -35 to 43 × 10 -6 until 20 days of age. In addition, the mock-up test results showed good workability and surface condition, and surface polishing is possible after 2 hours of consruction, which is considered to be very effective for Reducing work time.

본 연구는 스마트교육에 활용할 수 있는 공간정보 콘텐츠를 개발하고, 이를 활용하는데 목적 이 있다. 이를 위해 태블릿 컴퓨터와 HTML5, 증강현실(AR), 구글어스를 사용하여 공간정보 콘텐츠를 개발하고, 전자책의 형태로 재구성하여 스마트교육 환경이 구축된 학교 현장에서 사 용할 수 있도록 하였다. 또한 본 연구를 통해 개발된 공간정보 콘텐츠가 시사하는 바에 대해서 탐색하였다. 본 연구는 이러한 연구 결과를 바탕으로 초중등 사회과를 대상으로 전자교과서(전 자책)와 연관하여 적용할 수 있는 공간 정보를 활용한 디지털 콘텐츠 개발 사례를 제시하였다. 이는 앞으로 제기되는 창의적으로 비판적인 사고력 기반의 문제해결 능력의 교실 수업 적용과 디지털 콘텐츠와의 연결 측면에서 사회과 수업에 기여할 것으로 기대된다.

본 연구는 천연물질에서 유래한 반추위 메탄저감용 친환경 첨가제 개발을 위해 각기 다른 종균을 이용하여 발효한 세신 추출물의 항균활성, 항산화활성 및 in vitro 반추위 발효시험을 체계적으로 실시하였다. 접종된 균주들의 성장효율을 알아보기 위해 실시한 생균수측정 결과 L. curvatus NJ40 균주에서 유의적으로 높은 균주성장을 나타냈다(p<0.05). 항균활성측정 결과는 대조구 대비 L. curvatus NJ40 및 L. plantarum NJ45 균주와 발효된 세신 추출물이 병원균에 대한 항균효과를 나타내는 것으로 조사되었다. In vitro 반추위 발효실험에 세신 및 발효 세신 추출물을 적용한 결과, 휘발성 지방산 생성량 대비 반추위 메탄 저감효과가 나타났다. 특히 반추위 미생물 활력 및 사료이용 효율을 대표할 수 있는 휘발성 지방산 생성효율에 대한 부정적 효과 없이, 오히려 휘발성지방산 생성효율을 향상시키면서 반추위 메탄 저감효과를 가져올 수 있는 것으로 나타났다.