Fabry disease is an X-linked lysosomal storage disorder caused by GLA mutations, leading to a deficiency in α-Galactosidase A activity and subsequent accumulation of globotriaosylceramide (Gb3). This accumulation contributes to progressive multiorgan dysfunction, with cardiovascular complications, particularly endothelial dysfunction and left ventricular hypertrophy being major drivers of disease morbidity and mortality. Although enzyme replacement therapy is currently the standard treatment, its effectiveness is limited in addressing advanced cardiovascular pathology. To better understand Fabry-associated vascular and cardiac phenotypes, an isogenic human induced pluripotent stem cell (hiPSC) model in which GLA was knocked out was developed using CRISPR/ Cas9. GLA-knockout (GLA-KO) hiPSCs were differentiated into endothelial cells (ECs) and cardiomyocytes (CMs) to evaluate disease-relevant phenotypes in vitro . GLA-KO ECs exhibited normal morphology and differentiation capacity but showed markedly impaired tube formation, high expression of inflammatory genes ICAM1, VCAM1, and SELE, and increased mitochondrial and cytoplasmic reactive oxygen species levels. GLA-KO CMs demonstrated enlarged cell size and nuclear translocation of NFATC4, consistent with hypertrophic remodeling. Together, these findings recapitulate key features of Fabry vasculopathy and cardiomyopathy in a genetically defined, human-derived system. This platform enables direct investigation of Gb3-induced oxidative and inflammatory mechanisms and provides a valuable model for the preclinical evaluation of therapeutic strategies targeting the cardiovascular manifestations of Fabry disease.

Lumpy Skin Disease (LSD) and Foot-and-Mouth Disease (FMD) cause substantial economic losses on the livestock industry. Therefore, vaccinations have been implemented as the control strategy in endemic countries. However, the potential adverse effects of administering vaccines for both diseases simultaneously have not been thoroughly evaluated. The aim of this study was to assess the impact of vaccinating dairy cows with either or both LSD and FMD vaccines on milk production and physiological parameters such as milk temperature, rumination time and body weight. The experimental groups were divided into four according to the injection materials: 1) saline, 2) LSD vaccine, 3) FMD vaccine, and 4) both vaccines. The impact of vaccination on milk yield and physiological parameters was evaluated daily until 12 days post-vaccination, and milk components were analyzed twice, once per week. Among the experimental groups as well as each vaccine group, no statistically significant differences (p < 0.05) were observed at milk yield, milk components, or milk temperature. This suggests that simultaneous vaccination of LSD and FMD can be administered without adverse effects.

Maturity at harvest is the key factor influencing storage life and the final quality of fruit. This study examined how the firmness of ‘Sweet Gold’ kiwifruit at harvest affects its post-ripening characteristics to create a maturity chart. Throughout the storage period, firmness decreased in all categories of fruit: hard, medium, and soft. ‘Soft’ fruits lost 40% of their firmness within 2 days after harvest, whereas ‘hard’ fruits remained firmer than soft fruits throughout the storage period and had the least soluble solids content, indicating a slower ripening progression. The acidity of ‘soft’ kiwifruit was very low from the day of harvest, suggesting that it was utilized as a respiratory substrate during ripening. The a-values (indicating redness) for ‘soft’ fruits gradually increased until day 6, stabilizing thereafter. ‘Soft’ fruits exhibited the highest ethylene production rate throughout storage. They showed a climacteric rise in ethylene on day 13, compared to ‘medium’ and ‘hard’ fruits, which exhibited increases on days 19 and 21, respectively. This data can help determine the optimal ethylene treatment duration for ripening ‘Sweet Gold’ kiwifruit. The firmness of ‘Sweet Gold’ kiwifruit at harvest is a crucial factor in determining its marketability and can effectively serve as a maturity index to estimate its shelf life.

본 연구는 절화 국화의 수경재배와 토경관비재배 시 생육 특 성을 비교하기 위하여 실시하였다. 그 결과 초장, 절화의 생체중 과 건물중은 토경관비재배에서 증가하였다. 식물체 내 전질소, 칼륨 및 칼슘의 함량도 토경관비재배에서 더 높았다. 반면에 줄기 직경은 수경재배에서 3.7mm로 증가하였다. 또한 개화소 요일수도 토경관비재배보다 수경재배에서 3일 정도 단축되었 고, 줄기 1개당 꽃 수도 19.6개로 수경재배에서 더 많았다. 결과 적으로 토경관비재배에서 식물체 내 무기염류 함량이 높고, 초장이 길어지고 생체중, 건물중이 증가하였지만 수경재배보다 개화기는 지연되었다. 또한 토경관비재배보다 수경재배에서 줄 기 직경이나 꽃 수 등이 증가하였다. 따라서 재배자의 입장에서 국화 수경재배를 통해 양분과 수분 공급량의 조절에 의한 개화 기의 조절, 조기 개화 및 절화 품질 향상 등의 효과를 기대할 수 있을 것으로 판단된다.

Afoxolaner is an insecticide and acaricide that belongs to the isoxazoline chemical compound group. it has been used as an active pharmaceutical ingrdient in veterinary medicine to treat fleas and ticks in dogs. When patents expire between 2026 and 2066, it is expected that many products will be applied for approval as generic products, and reserch to establish accurate quality control methods must be conducted and managed. HPLC method was developed for the quantitative and qualitative of afoxolaner in veterinary medicinal products. The separation of active constituents for afoxolaner was achieved on a RP18 (4.6 x 150 mm, 5 μm) column using Water : Acetonitrile : MeOH (25:30:45 v/v/v) as mobile phase, with UV detection at 245 nm. The method was validated for specificity, linearity, accuracy and precision. All calibration curves showed good linearity (R2 of 0.999) within the concentration ranges (12.5 to 400 μg/mL). For accuracy, the recovery rate was calculated by spiking three concentrations of standard into the sample blank. The recovery rate was calculated to be 99.70~100.58%. Precision was measured 9 times repeatedly through intra-day, inter-day tests using standard. It showed excellent precision by satisfying the relative standard deviation of less than 2% both intra-day and inter-day. Limit of detection (LOD) and limit of quantitation (LOQ) were 2.0 μg/mL and 6.1 μg/mL, respectively. This method was successfully applied to analyzing afoxolaner drugs distributed in Korea. The HPLC method described in this study is accurate and reproducible and could be applied for the analysis of veterinary drugs of afoxolaner.

This study examines the impact of Strategic Emphasis (SE) on Enterprise Value (EV) in KOSDAQ 150 firms, exploring how the balance between Value Creation (VC) and Value Appropriation (VA) strategies influences Tobin's Q as a measure of EV across different industries. Using Mizik and Jacobson's Strategic Emphasis framework, this research conducted a panel regression analysis on financial and stock market data of KOSDAQ 150 firms over a decade (2014–2023). This study employed a comprehensive empirical framework that accounts for industry characteristics, firm profitability, and time effects to examine how different sectors respond to SE changes. The analysis revealed that SE significantly impacts EV, with varying effects across industries. For instance, VC strategies positively influenced EV in the Healthcare sector, whereas VA strategies proved more effective in the Consumer Discretionary sector. Additionally, the relationship between SE and EV was moderated by firm profitability and shaped by industry-specific factors. This study highlights the importance of choosing appropriate measures, such as Tobin's Q, when evaluating corporate strategies. The findings offer actionable insights for Korean venture firms, emphasizing that strategic resource allocation should be tailored to industry-specific dynamics and a firm’s financial context to maximize EV.