The CC chemokine receptor 5 (CCR5) is a G protein-coupled receptor that regulates chemotaxis and effector functions of immune cells. It also serves as the major co-receptor for the entry of human immunodeficiency virus (HIV). Recently, CCR5 inhibitors have been developed and used for the treatment or prevention of HIV infections. Additionally, it has been identified that CCR5 controls bone homeostasis by regulating osteoclastogenesis and the communication between osteoblasts and osteoclasts. However, the effects of CCR5 inhibition on bone tissue in elderly patients are unknown. This study aimed to examine the bone phenotype of aged CCR5 knockout (KO) mice. Femoral and tibial bones were isolated from 12-month and 18-month old wild-type (WT) and CCR5 KO mice, and microcomputed tomography and histology analyses were performed. Twelve-month-old CCR5 KO mice exhibited a decreased trabecular bone mass and cortical bone thickness in both femoral and tibial bones compared with agematched WT mice. Eighteen-month-old mice also showed a decreased trabecular bone mass in femurs compared with control WT mice, but not in tibial bones. Unlike in 12-month-old mice, the cortical margin of femurs and tibias in 18-month-old mice were rough, likely because they were aggravated by the deficiency of CCR5. Overall, our data suggest that the deficiency of CCR5 with aging can cause severe bone loss. When CCR5 inhibitors or CCR5 inactivating technologies are used in elderly patients, a preventive strategy for bone loss should be considered.

This study was conducted to determine the effect of six different leguminous seeds a) cowpea, b) white kidney bean c) soybean (Cheongja) d) soybean (Daepung) e) mung bean and f) azuki bean on the nymphal development, mortality, adult longevity, fecundity and hatchability of Riptortus pedestris (F.) (Hemiptera: Alydidae). Total developmental time ranged from 18 to 25 days; R. pedestris fed on white kidney bean took the longest time. There was no difference in mortality among the bugs fed on the legumes except those fed on white kidney bean which had the highest mortality (94%). Also, adult bugs fed on white kidney bean showed the shortest longevity. Total number of eggs laid by adult female and the hatchability of the eggs were highest from the bugs fed on cowpea. Higher reproductive success of R. pedestris fed on cowpea suggests that cowpea could be very suitable legume for the bug and it can be used as a trap crop against the bean bug.

Several pentatomids and an alydid are reported inflicting losses in several host plants ranging from leguminous crops to fruit orchards in Korea. Understanding their visual preference would help develop mass trapping or efficient monitoring device. Visual preference of the major pentatomids and an alydid bugs to different colors and light emitting diodes (LED) of different wavelengths were observed. Adults of pentatomid such as Halyomorpha halys Stål, Plautia Stali Scott, and Nezara sp., and an alydid Riptortus pedestris (F.) were exposed to seven different colors (black, yellow, green, blue, white, cream, orange) in a multiple choice tests. Also, adult and second instar nymphs of the bugs were released inside a hexagonal LED boxes consisting of LED lamps of six different wavelengths (375 to 630 nm). Most of the pentatomids showed highest preference to blue color followed by yellow and 530 nm LED. However, R. pedestris showed higher preference to the yellow color followed by blue and black. Second instar nymphs of all the tested species also showed higher preference to 530 nm. Their color and LED lamp preference would help in development of mass trapping device.

‘Orange Song’은 전북농업기술원에서 2011년 육성된 화단용 아시아틱나리 품종이다. 1999년 분홍색 아시아틱나리 ‘Chianti’을모본으로 하고 노랑색 아시아틱나리 ‘Madras’를 부본으로 하여주두수분법을 이용 인공 교배 후, 2002년 20개 실생묘 가운데개화 후 특성이 우수한 JaL02-46 계통을 선발하였다. 2003에서 2009년 까지 조직배양과 포장 양구를 통해 증식시킨 후 특성검정을 실시하였다. 2010~2011년 노지포장 적응시험을 위해전북농업기술원 익산포장과 남원, 고창 등 3곳의 노지포장에서 2년간 특성조사를 실시하였고 ‘Orange Song’으로 명명 되었다.노지에서 3지역 모두 2년간 평균 생존률이 98.7%로 높았고엽수도 48.3장으로 대조품종 Partner에 비해 8.4장이 많았다.개화기가 6월 22일로 중만생종에 속하고 진노랑색(RHS, O24A)이며 개화방향은 상향이고 내부에 반점이 있다. 초장이 50cm이하이고 잎마름병에 강한 특성을 가지고 있으며 수확한 구근의구주는 9.6cm, 무게는 17.7g으로 조사되었다.

Hematopoietic stem cells (HSCs) are the self‐renewing, multipotent progenitors that give rise to all types of mature blood cells. The hallmark properties of HSCs are the ability to balance self‐renewal versus differentiation cell fate decisions to provide sufficient primitive cells to sustain haematopoiesis, while generating more mature cells with specialized capacities. In the present experiment, we optimized the techniques for isolation and identification of hematopoietic stem cells from cow peripheral blood. The objective of this study was to optimize the more accurate methodology for separation of mononuclear cells (MNCs) from peripheral blood and identification of HSCs by using a specific cell surface marker i.e. CD34. A total 10 peripheral blood samples were collected from Holstein dairy cows from jugular vein. We used Ficoll 400 in different concentrations from 1 to 12% and Ficollpaque Plus (1.077 g/ml) at different centrifugation speed and time. After Giemsa staining, we found more than 98% recovery of monocytes with Ficollpaque Plus (1.077 g/ml). It was demonstrated that Ficollpaque Plus (1.077 g/ml) and centrifugation at 400xg for 30 min is the best method for separation of MNCs from bovine peripheral blood. Separated MNCs were immediately subjected for magnetic activated cell sorting (MACS) by using CD34 microbead kit. HSCs (CD34+ cells) recovery was 0.307% of peripheral blood. Peripheral blood MNCs and CD34+ cells were morphologically characterized by Giemsa staining. CD34+ cells were also confirmed by immunochemistry using FITC conjugated CD34 antibodies. HSCs were also confirmed by progenitor assay including burst forming unit‐erythroid (BFU‐E), colony forming cells‐ granulocyte (CFC‐G), colony forming cells‐ macrophage (CFC‐M), colony forming cells‐ granulocyte macrophage (CFU‐GM) and colony forming cells‐ granulocyte erythroid macrophage monocyte (CFCGEMM) on Methocult 4435.

This study was conducted in order to investigate the reduction activity of red ginseng extract (RGE; Panax ginseng, C. A. Meyer) on hydroxyl radical (·OH) using an electron spin resonance (ESR) spectrometer and spin-trapping techniques. ·OH generated by a Fenton Reaction System was trapped by 5, 5-dimethyl-l-pyrroline-N oxide (DMPO). The decay rate showed approximately pseudo-firs order kinetics over the period of measurement (by 10 min), and the half lifetime of the DMPO/·OH signal was estimated as approximately 8.15 min. However, the half lifetime of RGE/·OH was estimated as approximately 7.5 min, and the half lifetime of RGE was higher than that of DMPO/·OH adduct only. The order of reduction activities was ascorbic acid > N, Nʹ-dimethylthiourea (DMTU) > RGE > trolox > mannitol in the Fenton Reaction System. Thus, these observations indicate that RGE reaction with ·OH has relative reduction activity. The second-order rate constant of RGE/·OH may be 3.5~4.5 × 109 M-¹ ∙ S-¹.

본 논문에서는 산업 현장에서 비전기술을 이용한 객체의 검사나 분류에 사용되는 빠르고 효율적인 최소 사각형 근사화 방법을 대해서 소개한다. 주어진 객체의 최소 사각형 근사를 위해서 객체의 전체 외곽선을 사용하지 않고 n 개로 샘플링된 에지 점들에 대해서만 각도 히스토그램을 생성하고 이를 이용하여 객체의 장축과 단축을 계산한다. 최종 근사 사각형을 찾기 위해 이전 단계에서 얻어진 장축과 단축을 중심으로 하는 4개의 모서리점을 갖는 사각형을 찾는다. 이후에, 잡음에 의한 오차를 줄이기 위해 첫 번째 사각형의 각도를-1∼+1로 변형하여 추가적으로 2개의 후보 사각형을 생성한 후, 3개의 추정된 사각형과 원본 영상의 외곽선의 매칭 정도를 측정하여 오류가 최소화 되는 사각형을 최종 사각형 영역으로 선택한다. 제안된 방법을 몇 개의 실험데이터에 대해 실험한 결과 기존의 방법에 비해 빠르고 효율적인 최소 사각형 근사가 가능하였다.

Two species entomopathogenic fungi most widely used and valued in traditional Asian medical practice are Ophiocordyceps sinensis (formely Cordyceps sinensis) and Cordyceps militaris. Although O. sinensis may be the more famous and expensive fungus, it is also comparatively rare and cannot be grown or made to fruit readily in culture whereas C. militaris occurs worldwide, can be easily cultured, and is the easist of all Cordyceps species to fruit in culture. There is a well establihed cottage industry in Korea to produce C. militaris as a dietary supplement or even as a culinary ingredient used to promote improved health. Most of the Korean farmers raising C. militaris obtain fruiting bodies from silkworms that are injected with suspensions of hyphal bodies grown in liquid cultures. This study seeks to facilitate and to simplify the injection process used to produced this fungus by finding a simple cullture medium on which abundant supplies of the Lecanicillium conodial state of C. militaris are produced and can be used with simplified injection protocols involving spraying or dipping in conidial suspensions than the more material- and labor- intensive injection protocol. The studies to be repored include quantitative tests of conodial yields on varying carbon sources, varying nitrogen sources, and attempted to optimize the carbon/nitrogen ratio and pH of the medium for conidial production.

To investigate the effect of carnosine on exhaustive exercise, swimming tests were conducted weekly with loads corresponding to 5% of body weight attached to the tails of mice, and the swimming time to exhaustion was measured. Eighty male ICR mice were divided into four groups, to which carnosine was administered at doses of 0 (control), 10, 50, and 250 mg/kg/day, respectively, for a period of four weeks. At the end of swimming exercise challenges, serum biochemistry, oxidative stress enzyme activity, and antioxidant enzyme activity in tissues were determined. Treatment with 250 mg/kg carnosine resulted in a significant increase in swimming times to exhaustion, compared to the control group in the first (P<0.01) and third week (P<0.05). Significantly lower serum lactate levels were observed after the swimming exercise in the carnosine-treated groups (10 and 250 mg/kg), compared with the control (P<0.01). Malondialdehyde levels in the liver (10 and 50 mg/kg carnosine treated groups) and skeletal muscle (250 mg/kg carnosine treated group) were significantly lower, compared with the control (P<0.05). Significantly lower protein carbonyl levels in skeletal muscle were observed in the 50 and 250 mg/kg carnosine treated groups, compared with the control (P<0.01). Superoxide dismutase and glutathione peroxidase activities in skeletal muscle did not differ significantly among the groups. These results indicate that carnosine may improve swimming exercise capacity by attenuating production of lactate and reducing oxidative stress in mice.