If radioactive plumes are released outside due to loss of containment building integrity during a nuclear power plant accident, these materials might travel with the wind, affecting both the surrounding environment and neighboring countries. In China, most nuclear power plants are located on the eastern coast. Consequently, a radioactive plume generated during an accident could negatively impact even the western part of the Korean Peninsula due to westerly winds. To detect such problems early, respond quickly, and protect residents, a system that can monitor aerial radiation under normal conditions is needed. Additionally, a detection system that can operate in real-time in an emergencies conditions is required. The current method for aerial radiation measurement takes environmental radiation data from a monitoring post 1.5 m above the ground and converts it to altitude. To measure actual aerial radiation, an expansive area is surveyed by aircraft. However, this approach is both time-consuming and expensive. Thus, to monitor radioactive plumes influenced by environmental factors like wind, we need a radiation detector that can gauge both radioactivity and directionality. In this study, we developed a radiation detector capable of assessing both the radioactivity and directionality of a radioactive plume and conducted its performance evaluation. We miniaturized the radiation detector using a CZT (Cadmium Zinc Telluride) sensor, enabling its mounting on unmanned aerial vehicles like drones. It is configured with multi-channels to measure directionality of a radioactive plumes. For performance evaluation, we positioned two-channel CZT sensors at 90 degrees and measured the energy spectrum for angle and distance using a disk-type radioactive isotope. Using this method, we compared and analyzed the directionality performance of the multi-channel radiation detector. We also confirmed its capability to discern specific radioactivity information and nuclide types in actual radioactive plumes. Our future research direction involves mounting the multi-channel radiation detector on a drone. We aim to gather actual aerial radiation data from sensors positioned in various directions.

This study focuses on the development of coatings designed for storage containers used in the management of radioactive waste. The primary objective is to enhance the shielding performance of these containers against either gamma or neutron radiation. Shielding against these types of radiation is essential to ensure the safety of personnel and the environment. In this study, tungsten and boron cabide coating specimens were manufactured using the HVOF (High-Velocity Oxy Fuel) technuqe. These coatings act as an additional layer of protection for the storage containers, effectively absorbing and attenuating gamma and neutron radiation. The fabricated tungsten and boron carbide coating specimens were evaluated using two different testing methods. The first experiment evaluates the effectiveness of a radiation shielding coating on cold-rolled steel surfaces, achieved by applying a mixture of WC (Tungsten Carbide) powders. WC-based coating specimens, featuring different ratios, were prepared and preliminarily assessed for their radiation shielding capabilities. In the gamma-ray shielding test, Cs-137 was utilized as the radiation source. The coating thickness remained constant at 250 μm. Based on the test results, the attenuation ratio and shielding rate for each coated specimen were calculated. It was observed that the gammaray shielding rate exhibited relatively higher shielding performance as the WC content increased. This observation aligns with our findings from the gamma-ray shielding test and underscores the potential benefits of increasing the tungsten content in the coating. In the second experiment, a neutron shielding material was created by applying a 100 μm-thick layer of B4C (Boron Carbide) onto 316SS. The thermal neutron (AmBe) shielding test results demonstrated an approximate shielding rate of 27%. The thermal neutron shielding rate was confirmed to exceed 99.9% in the 1.5 cm thick SiC+B4C bulk plate. This indicates a significant reduction in required volume. This study establishes that these coatings enhance the gamma-ray and neutron shielding effectiveness of storage containers designed for managing radioactive waste. In the future, we plan to conduct a comparative evaluation of the radiation shielding properties to optimize the coating conditions and ensure optimal shielding effectiveness.

Minuartia laricina (L.) Mattf. is a Korean native plant with high potential as a commercial flowering potted plant due to its compactness and long flowering duration. However, because this plant is a groundcover, it is susceptible to lodging and leggy growth. Therefore, this study investigated the effects of plant growth retardants (PGRs) on the inhibition of stem elongation and flowering characteristics of M. laricina. Commercial products, Trimmit, Cycocel, and B-Nine, were used for the exogenous PGR application of paclobutrazol (PBZ), chlormequat chloride (CCC), and daminozide (DMZ), respectively. Application concentrations were 50 and 100 mg·L-1 for PBZ; 100, 500, and 1,000 mg･L-1 for CCC; and 500, 1,000, and 2,000 mg·L-1 for DMZ. Paclobutrazol was the only PGR that inhibited stem elongation. The stem lengths of the plants treated with 50 or 100 mg·L-1 PBZ were 2.2 cm (13%) or 9.8 cm (57%) shorter, respectively, than those of the control. 50 mg·L-1 PBZ retarded stem growth effectively without negatively affecting flowering or other growth parameters, whereas 100 mg·L-1 PBZ caused excessive dwarfing and significantly reduced flowering by 59%. CCC and DMZ applications were ineffective for growth control. Flowering time was accelerated with most PGRs, except for 2,000 mg·L-1 DMZ, reducing the time to flowering by 2–8 days. These results indicate that the stem growth of M. laricina was successfully inhibited with PBZ but not with CCC or DMZ. Thus, we concluded that a single application of 50 mg·L-1 PBZ or similar treatment is effective in miniaturizing M. laricina without causing harm to its growth or aesthetic value, such as the flower number. Additionally, because CCC and DMZ are not persistent in the growing medium, testing multiple application times for these PGRs is crucial.

본 연구는 호남지방 소비자 들의 곤충 제품과 관련하여 식품구매 행동, 곤충제품에 대한 인식, 식용, 학습, 애완용, 사료용 곤충에 대한 인식 등에 대한 기초 자료를 얻고자 수행하였다. 연구의 대상은 광주, 전주, 순천 등 대도시 학생과 성인 230여명을 대상으로 2018년 5월부터 9월까지 설문조사를 실시하였다. 설문조사 결과, 새로운 음식을 맛보는 소비자는 36%인고 맛보지 않는 소비자는 32%이지만 새로운 음식을 신뢰하는 소비자는 19%로 신뢰하지 않은 소비자 45%보다 더 적었으며 음식 재료를 모르면 맛보지 않은 소비자가 47%로 많지만 먹어 본적 없는 음식을 꺼리지 않고(46%), 까다롭지 않으며(45%), 무엇이든 잘 먹는(57%) 소비자가 더 많았다. 곤충 제품에 대한 인식은 곤충을 잘 알고 있으며(97%), 체험해보거나(89%), 구입해본 적이 있는(59%) 소비자가 더 많아 소비자들의 거부감이 크지 않음을 알 수 있었으며, 구입장소는 다양하고 주로 TV, 라디오를 통해(48%) 알게 되며 체험 종류나(59%), 구입 용도(63%)는 대부분 식,약용 곤충이었다. 식용곤충 구입은 어쩌다 한번이 79%로 아직까지 주기적으로 구입은 하지 않았으며 멋져 보이거나(54%), 건강을 생각하여(28%) 구입하는데 요리재료(46%), 건조, 튀김(24%) 형태로 누에(74%), 갈색거저리(6%), 흰점박이꽃무지(5%) 등을 구입하였다. 가격 만족도는 47%이며 품질(52%), 구입경로(53%), 맛(70%) 만족도도 긍정적으로 높았다.

농촌진흥청 개발 품종인 토종벌 신품종(질병저항성 우수계통)에 대해 전남지역에서의 형질특성(봉세발달, 마리당 수밀력, 청소력 등)을 조사하였다. 질병저항성 계통(RS)으로 육성된 토종벌의 유밀기 마리당 수밀력은 들어오는 일벌의 무게가 80.89±8.95mg, 나가는 일벌의 무게가 63.56±8.90mg으로 조사되었고, 비저항성계통(NRS)은 각각 83.22±1.39, 66.67±1.20mg으로 조사되어 RS에서 그 무게차가 0.7mg 더 높았으나 통계적 유의차는 없었다. Pin killed test에 의한 사충제거능력(청소력)은 제거하지 못한 유충잔존율이 RS에서 12시간과 24시간 경과시 14.00±10.39와 7.00±3.46으로, NRS에서는 20.33±14.29, 13.33±10.41%로 조사되었으며, 특히 RS와 NRS의 사충 비제거율이 24시간 경과하였을 때 4.67±2.08, 8.33±7.77%로 RS가 우수한 특성을 보였다. 신규여왕벌 입식 후 봉군세력발달은 NRS에 비하여 RS의 일벌수, 번데기 및 유충수의 발달율이 안정적이고 다소 빠르게 증가하였다.

기후변화, 외래병해충발생, 밀원수 감소 등 여러 요인으로 인해 우리 나라의 양봉농가는 벌꿀생산량 및 농가소득이 감소하는 추세이다. 또한 밀원이 없는 시기에 가축용 사료가 아닌 고가의 식용설탕을 꿀벌의 인공사료로 급여하고 있어 농가는 ‘생산비 상승’과 ‘밀원수 부족’을 국내양봉업이 직면한 가장 큰 문제점으로 지적하고 있다. 이에 따른 양봉농가의 생산비를 절감하고 꿀벌의 생리·생태의 개선효과를 가지는 저가형 당액사료개발을 위해 농산부산물에서 추출한 천연당을 봉군에 적용시험을 추진하였다. 양파(OS-1, OS-2), 양배추(CS-1, CS-2), 바나나(Banana)부산물에서 추출한 천연당의 봉군당 평균먹이소모량(mL)은 설탕당액(대조구) 처리구와 비교시 바나나추출당액 급여구에서 5500.00±0.00, 5133.33±404.15로 유사하였다. 그러나 꿀벌의 봉세발달은 대조구에 비해 21006.7±2137, 17403.3±2257마리로 조사되어 낮은 경향을 나타내었고 수명조사 결과는 큰 차이가 없었다. 농산부산물을 이용한 천연당은 우선 꿀벌의 기호도 향상을 우선으로 개선시켜야 할 것으로 보인다.

Gryllus bimaculatus is one of many cricket species known as field crickets. Also known as the African or Mediterranean field cricket or as the two-spotted cricket, it can be discriminated from other Gryllus species by the two dot-like marks on the base of its wings. G.bimaculatus is a subtropical insect and widely distributed from Africa to south Asia. After into the country, The species are popular for use as a food source for insectivorous animals like spiders and reptiles kept as pets. In 2016, was approved as a general food ingredient by Korean Ministry of Food and Drug Safety. However, domestic research on G.bimaculatus is still in its study is beginning stages. and analyze nutrtion elements as per dry conditions for processing&production of G.bimaculatus. The growth stage passing by 50 days after hatching was highest in general component and amino acid contents. The crickets passing by 50 days after hatching was highest 77% crude protein in condition hotair drying 60℃, 23% crude fat in condition freeze drying.

Oxya chinensis sinuosa Mistshenko(Orthoptera:Acrididae) was lived in clean and non polluted area. grasshopper was approved as a general food ingredient by Korean Ministry of Food and Drug Safety. general component contents was 67~75% crude protein, 5~11% crude fat in a dried state. grasshopper is a high protein food. amino acids is high. In the composition and content of amino acids, which consists of the protein is the hightes alanine, glycine(soft and sweet) and arginine, histidine(essential amino acid of children) consequently, we developed a snack for children added with grasshopper powder. general composition of snack is 8~11% crude protein, 0.2~0.75% crude fat. The protein content increased with increasing addition ratio. 3% grasshopper powder was the best in general preference.

Toll and IMD pathways play an important role in producing antimicrobial peptides (AMPs) through NF-κB in insects. The functions of IκB kinase (IKK) complex regulating the NF-κB signaling cascade have not yet been investigated in Tenebrio model. Here, we identified TmIKK-β (or TmIrd5) which contains 2,112 bp encoding 703 amino acid residues. Domain analysis shows that TmIKK-β contains one Serine/Threonine protein kinases catalytic domain. Developmental expression patterns indicate that TmIKK- β gene was highly expressed in early pupal (P1) and adult (A5) stages. Tissue specific profiles show that TmIKK-β was highly expressed in the integuments in last instar larvae, and fat body and hemocytes in 5 day-old adults. TmIKK-β1 transcripts were strongly induced at 3 and 12 h-post injection of E. coli, and 3 h-post injection of S. aureus or C. albicans in hemocytes. In gut, TmIKK-β transcripts were slightly induced by E. coli (at 6, 9 and 24 h) and C. albicans (at 24 h), while it was not induced by S. aureus challenge. Moreover, it was highly induced at 6 h-post injection of E. coli and then it was gradually decreased in the fat body. To understand the immunological role of TmIKK-β, gene specific RNAi and mortality assay was performed. Depletion of TmIKK-β mRNA leads to increase microbial susceptibility of larvae against E. coli, S. aureus and C. albicans. In addition, induction patterns of fourteen AMP genes in response to microbial challenge was tissue specifically investigated in TmIKK-β–silenced T. molitor larvae. The results suggest that expression of ten AMP genes out of fourteen genes were drastically decreased by TmIKK-β RNAi in fat body, suggesting that TmIKK-β plays an important role in antimicrobial innate immune responses.

Autophagy is an important self-eating process to eliminate damaged or unused organelles. We identified nine autophagy-related genes (Atg) including AaAtg-1, -3, -4b, -4d, -5, -6, -8, -12 and -13 from the Asian tiger mosquito, Aedes albopictus. Developmental expression patterns indicate that mRNA levels of AaAtg-1, -3, -4b, -4d, -5, -6, -12 and -13 were highly expressed in egg, whereas expression of AaAtg8 was high in 1stand3rdinstarlarvalstages. TissuespecificexpressionofthesegenesindicatesthatAaAtg1 was highly expressed in thorax and midgut in blood-fed adult female mosquitoes (BF), and head and thorax in sugar fed adult female mosquitoes (SF). Transcript level of AaAtg3 was high in thorax in BF, but head, thorax and Malpighian tubules in SF. AaAtg4b, -4d mRNA levels were significantly high in Malpighian tubules in BF, and head in SF, respectively. AaAtg-5 and -6 transcripts were highly expressed in head in BF, and expression of AaAtg-8 was high in Malpighian tubules in BF. Levels of AaAtg-12 and -13 mRNAs were significantly high in head and midgut in BF. Induction patterns of AaAtg genes against pathogens showed that AaAtg-1, -3, -4b, -8, -12 and -13 were strongly induced at 6 h-post injection of S. aureus, and mRNA levels of AaAtg-1, -3 and -13 were significantly induced by E. coli challenge after 3 h-post injection in SF abdominal carcass. In SF midgut, AaAtg-1, -3, -4b, -4d, -5, -6, -12 and -13 transcripts were drastically induced at 9 h-injection of E. coli and S. aureus, while expression of AaAtg-8 was highly induced by S. aureus and C. albicans at 9 h-post injection. Each AaAtg gene was slightly induced by E. coli, S. aureus or C. albicans at different time points in abdominal carcass in BF. Interestingly, AaAtg-8 was not induced by microbial challenge. While eight other Atg genes except AaAtg-8 were highly influenced by S. aureus at 6 and 9 h-post injection, E. coli at 3 h-post-treatment, and 3, 6, and 9 h-post inoculation. In the future, we will characterize the functional roles of autophagy during mosquito-microbes interaction.

It has been well known that IKK-β, -ε and –γ play a pivotal role in IMD pathway. In this study, TmIKK-ε was identified and their functions in countering pathogenic infections were investigated. We identified TmIKK-ε gene which including 2,196 bp nucleotides (encoding 731 amino acid residues). Domain analysis of TmIKK-ε indicates that there is one Serine/Threonine protein kinases catalytic domain. TmIKK-ε gene was highly expressed in 2 day-old pupal stage and the expression was gradually decreased until 1 day-old adults. Then the expression was slightly increased until 4 day-old adult stage. Tissue specific expression of TmIKK-ε mRNA was high in the gut, integuments and hemocytes in last instar larvae, and fat body, Malpighian tubules and testis in 5-daysold adult. In hemocytes, TmIKK-ε was drastically induced by E. coli injection after 3 h and by S. aureus at 3 and 12 h-post injection. In gut, expression level of TmIKK-ε was high at 6 h-post injection of microbial injection. Expression of TmIKK-ε in fat body was drastically induced by E. coli at 3 and 24 h-post injection while it was not significantly induced by S. aureus and C. albicans. To understand the immunological role of TmIKK-ε, gene specific RNAi and mortality assay were performed. TmIKK-ε RNAi caused increased larval mortality against E. coli, not S. aureus and C. albicans. Finally, to investigate the induction patterns of Tenebrio fourteen AMP genes in response TmIKK-ε RNAi, three microorganisms were treated into TmIKK-ε-silenced T. molitor larvae. Nine out of fourteen AMP genes were not induced by microbial challenge in TmIKK-β dsRNA-injected group. Taken together, our results indicate that TmIKK-ε may regulates nine antimicrobial peptide genes in response to microbial challenge in T. molitor fat body.

Host defense against pathogen invasion highly relies on immune defense machinery that is controlled by the nuclear factor-κB (NF-κB) of transcription factors. The Toll pathway are well known as an insect innate immune mechanism to protect host itself from invaded pathogens. Basically, in the edible insect, Tenebrio molitor, the Toll pathway is primarily activated by polymeric Lys-type peptidoglycans (PGNs), and components of fungal cell walls, β-1,3-glucan. Based on the current studies, the tremendous study has been focused on recognition and subsequent activation of spätzle in haemolymph, hence, there is a grave gap for intracellular event. Herein, in order to understand intracellular event of Toll signaling pathway, the Dorsal gene were identified. Moreover, domain analyses of TmDorsal2 gene indicate that there are two major domains such as Rel homology domain (RHD), ig-like, plexins, and transcription factors (IPT) domains. Based on the achieved results, TmDorsal2 mRNA was highly expressed in 1-day old pupa. Furthermore, TmDorsal2 was highly expressed in Malpighian tubules and fat body in last instar larvae (LL), and likewise mainly expressed in Malpighian tubules during adult 5-day old period, also the lowest expression of TmDorsal2 was observed in gonads. Moreover, TmDorsal2 mRNA levels after infection with E. coli appreciably went up at 6 and 9h time points. To investigate the effects of TmDorsal2 RNAi on larval susceptibility against various pathogens namely E. coli, S. aureus or C.albicans, dsRNA of TmDorsal2 has been synthesized the larvae dissected after 24h. As a result, TmAttacin1a, 1b and 2, TmDefencine1 and 2, TmTenecin1, 2, 3 and 4, TmCecropin2, TmColeoptericin1 and 2, Thaumatin-like protein 1 and 2 markedly reduced in the gut after injecting all mentioned microbes. In contrast, TmTenecin 2, Thaumatin-like protein 1 and 2 strikingly increased after microbe injection in the fat body. Interestingly, the most AMPs gene expression in whole body experimental case were upregulated. On the horizon, we will investigate effects of TmDorsal1 RNAi on larval susceptibility against various pathogens. Taken together, our studies may aid to understand insect innate immunity.

벼메뚜기는 분류학적으로 메뚜기목(Orthoptera), 메뚜기과(Acrididae)에 속하는 곤충으로 작물에 피해를 주는 해충으로 인식하고 있지만 예로부터 단백질 보충을 위하여 채집하여 튀기거나 볶음요리 로 이용해 왔다. 벼메뚜기는 갈색거저리, 쌍별귀뚜라미 등과 함께 식품공전에 등록되어 있으나 1년 1세대로 가을철에 채집하여 이용하는 실정으로 공급확대에 한계가 있다. 최근 우리나라에서 벼메뚜기를 사육하고자 하는 농가와 다양한 용도로 가공을 통해 이용하려는 수요가 점차 증가하고 있으나 연중사육기술이 개발되지 않아 대량사육하는 농가가 거의 없는 실정이다. 이에 벼메뚜기의 연중대량사육기술 뿐만 아니라 인공사료 및 자동화 사육시설 개발에 따른 생산비 절감 기술 개발이 필요한 실정이다. 벼메뚜기의 먹이로는 여름작물로 옥수수, 수단그래스, 겨울작물로 밀, 보리 등의 볏과작물이 있으며 많은 양의 생엽과 발육에 적합한 작물의 선발 및 볏과작물의 통곡실 가루를 이용한 인공사료의 개발로 먹이공급 부족 시 15~20일 급여 가능하며, 벼메뚜기 사육시설로 단독형, 연결형, 절충형 등의 사육시설을 개발하여 연결형 사육시설의 수확량과 생존율이 가장 높았으며, 예취 급이 노동력이 단독형의 16% 소요되었다.

IKK-γ is an essential protein to form IKK complex which regulate NF-κB. We identified TmIKK-γ (or TmKenny) gene which has 1,521 bp of nucleotides encoding 506 amino acid residues. Domain analysis of TmIKK-γ shows that there are one NF-κB essential modulator (NEMO) domain and a leucine zipper domain. Expression of TmIKK-γ gene was gradually increased from egg to 2-day-old pupal stage, dramatically decreased until 7 day-old pupal stage, and then it was gradually increased. TmIKK-γ transcripts were highly expressed in fat body and hemocytes in late instar larvae and integuments, fat body and Malpighian tubules in 5 day-old adult. TmIKK-γ was drastically induced by E. coli after 3 h challenges and by S. aureus at 3 and 12 h-post injection in hemocytes. TmIKK-γ was not induced by C. albicans although it was significantly induced by E. coli (at 3, 6 and 24 h) and S. aureus (at 9 h) in gut. In fat body, expression of TmIKK-γ was drastically induced by E. coli at 3 and 24 h-post injection while it was not significantly induced by S. aureus and C. albicans. To understand the immunological role of TmIKK-γ, gene specific RNAi and mortality assay was performed. larval mortality against microbial challenge was dramatically increased by TmIKK-γ RNAi. Furthermore, we investigate the tissue specific induction patterns of fourteen AMP genes in response TmIKK-γ dsRNA-treatment. In fat body, ten AMP genes out of fourteen was not significantly induced by microbial challenge in TmIKK-γ dsRNA-treated group. Based on these results, TmIKK-γ might play an important role in antimicrobial innate immune responses in Tenebrio molitor.

In this study, eye movements of drivers on expressway ramps were comprehensively investigated using raw data from driving simulator and eye tracker(MyGaze) deducted though the previous study. Based on the literature review, several parameters including number of fixations, fixation time, horizontal & vertical eye movements, and gaze position were selected. With them, eye movements of older drivers and young drivers were compared and investigated. Average fixation time of older drivers is shorter and tend to focus more on the inside road edge for both left and right turning ramps comparing young drivers. Also, they tend to look wider at right turning ramp. All driver’s fixation points were spread along the inside road edge for both left and right turning ramps. Currently, road delineating facilities are installed on the side where centrifugal force is applied. However, considering the result, it should be installed where centripetal force is applied. In addition, road line marking management should be more taken care of. For future study, vehicle’s speed is also in need of consideration with eye movement.

Gryllus bimaculatus is one of many cricket species known as field crickets. Also known as the African or Mediterranean field cricket or as the two-spotted cricket, it can be discriminated from other Gryllus species by the two dot-like marks on the base of its wings. G.bimaculatus is a subtropical insect and widely distributed from Africa to south Asia. After into the country, The species are popular for use as a food source for insectivorous animals like spiders and reptiles kept as pets. In 2016, G.bimaculatus was approved as a general food ingredient by Korean Ministry of Food and Drug Safety. However, domestic research on G.bimaculatus is still in its study is beginning stages. G.bimaculatus is possible species to year-round rearing without storage condition. but The aim of the present study prepares for in case of problems such as breeding space, labor cost etc. In the laboratory condition at 28±2℃ and 50% relative humidity under 10h light, 12h dark photoperiod, Adult crickets oviposit at soaked flower foam for 24 hours. The experiment on the hatching of the eggs showed that eggs could be stocked at 16℃ for 10 days with 7 day pre-period after laying, representing 85% hatchability.

아메리카왕거저리의 사육기간을 단축과 기능성 향상을 위해 밀기울에 다양한 먹이를 보조사료로 공급하여 발륙기 간과 무게를 측정한 결과, 먹이종류별 아메리카왕거저리의 발육기간은 조단백질함량이 많은 배합사료와 밀기울에서 80.8일과 75.6일로 가장 짧았으며 버섯배지는 160일이상 소요되었으며 왕겨의 생충율은 85%로 가장 낮아 버섯폐배지 와 왕겨는 먹이원으로 적절하지 않았다. 보조 먹이원 선발을 위해 할맥, 압맥, 배추, 백련초, 파프리카, 개똥쑥을 20~80% 밀기울에 첨가한 결과 압맥과 할맥은 정상적으로 생장하지만 백련초, 개똥쑥 혼합에서는 접종 직후부터 죽은 개체들이 출현하여 생충율이 12.9%, 10.8%로 아주 낮게 나타났으며 배추와 파프리카도 24.2~38.8%로 낮아 보조사료로 적합하지 않았다. 들깨와 콩가루 역시 생충율이 높지 않아 1, 2차 시험결과 할맥, 압맥과 메밀이 보조사료로 적합하였다.

This study was performed to investigate how changing the period of light and dark influences the vegetative growth and the photosynthesis of Doritaenopsis Queen Beer ‘Mantefon’. Clones of Dtps. Queen Beer ‘Mantefon’ at 4-month-old stage were grown in a closed-plant factory system with four different light/dark cycles; 06/06 h, 08/08 h, 10/10 h, and 12/12 h. Temperature and relative humidity were set at 28oC and 80%, respectively, with a photosynthetic photon flux density of 160 ± 10 μmol·m-2·s-1. Repetitive measurements showed that the leaf length and the leaf width were the longest under 12/12 h closely followed by 10/10 h. The fresh weight and the dry weight of leaves and roots were the heaviest at 10/10 h treated samples. Different CO2 uptake patterns were observed from different light/dark cycles. Under 10/10 h and 12/12 h treatments, the CO2 uptake started at early dark period. When the light/dark cycles were shortened to 06/06 h and 08/08 h, the CO2 uptake started at the middle of dark period. Total CO2 uptake amounts were the highest under 12/12 h treatment followed by 10/10 h, 06/06 h, and 08/08 h treatments. Quantitative measurements showed that the vegetative growths under 10/10 h treatment were comparable with that of 12/12 h treatment. These studies indicated that manipulating light/dark can modify the photosynthesis patterns and vegetative growth of Dtps. Queen Beer ‘Mantefon’, resulting in the reduction of the production period.