향후 우리 사회의 혁신적 변화를 가져올 휴대용 전자기기, 전기자동차 및 스마트 그리드 에너지 저장장치 등의 비약적인 발전에 따라, 그 전원으로서 리튬이차전지에 대한 관심이 더욱 증대하고 있다. 본 총설에서는, 리튬이차전지 핵심 소재 중 하나인 분리막에 대해 기공 구조 및 물리화학적 물성 관점에서 고찰하고, 이와 함께 최신 연구 동향을 소개하고자 한다. 리튬이차전지 분리막은 양극과 음극 사이에 위치하는 다공성 막으로서, 두 전극 간의 전기적 단락을 방지하고, 이온의 흐름을 가능하게 하는 기능을 갖는다. 분리막 자체는 전지 내 전기화학 반응에는 직접적으로 참여하지 않으나, 앞서 언급한 기능들에 의해 전지 성능 및 안전성에 큰 영향을 끼친다. 최근 들어, 이러한 분리막의 기본 특성 이외에, 전지 안전성 강화 및 금속 이온 흡착 등을 비롯한 다양한 기능 부여를 위한 노력들이 활발히 진행되고 있다. 본 총설에서는 현재 상업화된 폴리올 레핀 분리막에 대한 이해를 토대로, 개질 폴리올레핀 분리막, 부직포 분리막, 세라믹 복합 분리막 및 화학 활성 분리막 등으 로 대표되는 최신 분리막 기술들을, 차세대 전지 개발 방향과 관련 지어 기술하고자 한다.

Coastal dune wetland has an important ecological implication in that it could be closely related to the coastal dune ecosystem by providing various ecological habitats as well as ecological corridor. This paper aims to investigate the hydrological characteristics and aquatic biota of Mokgimiyeonmot (pond), coastal dune wetland in Gulupdo (island). Mokgimiyeonmot located in the lowland dune flat areas has six months long hydro period a year and can be categorized into wet dune slack type coastal dune wetland. As a result of this study, two species of freshwater fishes, two species of amphibia, and 35 species of benthic macro-invertebrates are recognized. Talitridae sp. belonging to Amphipoda also recognized in this survey for the first time from Korea. The results of this study are expected to be basic data necessary to understand the aquatic ecosystems and manage the ecological function of coastal dune field.

Sperm-mediated gene transfer (SMGT) can be used to transfer exogenous DNA into the oocyte at fertilization. The main objective of this study was to assess efficiency of transferring mitochondrial DNA (mtDNA) fragment into boar spermatozoa in either presence or absence of liposome and quality of transfected spermatozoa. The mtDNA of chicken liver was isolated and purified by phenol and alkaline lysis extraction, and it was inserted to plasmid. The genome of transfected spermatozoa treated with DNase Ⅰ was purified by alkaline lysis, and then amplified by the PCR analysis. After electrophoresis, DNA quantitation of each well was calculated by comparison of the band intensity with standard. As a result, exogenous DNA was composed of mtDNA fragment (1.2 kb) and plasmid (2.7 kb). On the other hand, efficiency of transfection by liposome (9.0±0.34 ng/l) in SMGT was higher than that by DNA solution (6.9±0.53 ng/l). However, there was no significant difference. Transfering exogenous DNA into spermatozoa was completed within 90 min of incubation. In another experiment, there were significant (p<0.05) differences between transfected spermatozoa using both DNA solution and DNA/liposome complexes with untreated spermatozoa for viability (70.8±1.80 and 68.0±2.16% vs. 83.3±1.69%, respectively) and motility (78.7±1.59 and 79.3±2.14% vs. 86.7±1.59%, respectively). This study indicates that exogenous mtDNA can be efficiently transferred into boar spermatozoa regardless of the presence of liposome, and transfected spermatozoa can also use insemination and in vitro fertilization to generate transgenic pig.

본 연구는 OPS 기법에 의한 돼지 수정란의 동결-융해 시 수정란의 발달 단계와 superoxide dismutase (SOD)가 수정란의 생존능력에 미치는 영향을 검토하였다. 돼지 체외수정 배반포는 OPS방법에 의해 동결 후 융해하여 0~10unitsml의 SOD 존재 하에 48시간 체외배양하였다. 동결-융해 후 형태학적으로 정상적인 수정란의 비율은 초기, 중기 및 확장배반포간에 유의적인 차이는 인정되지 않았다(30.8~38.6%). 그러나 발육단계가 높을수록 형태적으로 정상인 수정란의 비율이 높은 경향을 나타냈다. 수정란의 융해 후 48시간 추가 배양했을 때, 발육이 진행된 수정란은 후기배반포기에 동결한 수정란이 38.7%로 유의적으로 높았으며(P<0.05), 1 unit/ml의 SOD를 첨가한 경우 비교적 높은 생존율을 나타내었다. 본 연구의 결과로부터, 수정란의 OPS방법에 의한 동결-융해 후 생존성의 향상을 위해서는 후기배반포기 단계에 동결하는 것이 유리하며, SOD의 첨가는 수정란의 손상을 어느 정도 방지할 수 있을 것으로 사료된다.

Recently with increase tendency of direct sowing cultivation, lodging problem which affect to the high quality rice plant is again raising its ahead. In order to reduce this lodging problem there is a chemical method which uses plant growth retardant. This experiment was conducted to evaluate the effect of lodging reduction as affected by plant growth retardant which is IBP-Metconazole (IPM) in direct-seeded rice on flooded paddy field. IPM treatment carried out 50, 40, 30 days before heading. Hexaconazole treatment carried out 30 days before heading. IPM treatment 30 days before heading resulted the shortest plant culm among the IPM treatments. 4 internode length was shorter by 3.75㎝ in IPM treatmenst than in control. breaking strength was not a difference from between IPM treatments but breaking strength was significantly increased by IPM treatments than control. IPM treatments increased yield 10~11% as compared with control. The protein contents of flag leaves by 2-DE were visually higher in the plot treated with the IPM application than in the plot control plot. The proteome approach to elucidate the mechanism which inhibited gibberellin synthase will be future performed in the on-going research.