This study was undertaken to evaluate the quality characteristics and antioxidant characteristics of commercially available mixed grains in Korea. The quality characteristics of mixed grain products studied were the mixing ratio, water binding capacity, water solubility, swelling power, and pasting characteristics. The antioxidant characteristics assessed the total polyphenol, flavonoid contents, DPPH and ABTS radical scavenging activities. The mixing ratio of commercially available mixed grain products consisted of 5~25 kinds of grains, with maximum products containing 15-grain products. The water binding capacity, water solubility, and swelling power in commercially available mixed grain products were 99.83~122.83%, 6.91~39.26% and 7.76~86.92%, respectively. The peak, trough, breakdown, final and setback viscosity were 31.53±20.17 RVU, 25.24±13.22 RVU, 6.29±7.43 RVU, 50.27±25.84 RVU and 18.74±8.68 RVU, respectively. Total polyphenol and flavonoid contents were 817.14~2,524.29 μg GAE/g and 06.36~1,099.09 μg CE/g, respectively. The DPPH and ABTS radical scavenging activities were 31.91~151.70 mg TE/100 g and 28.09~119.92 mg TE/100 g, respectively. Products with high phenol content and radical scavenging activity were found to contain greater proportion of brown rice, colored rice, barley and soybean.

Euzophera batangensis (Lepidotera: Pyralidae) is seriously damaging trunks or branches of persimmon tree (Diospyros sp.). We tested the attractiveness of (Z)-9-tetradecen-1-ol (Z9-14OH) and (Z9,E12)-tetradeca- 9,12-dien-1-ol (Z9,E12-14OH) with single or blended baits at southern parts of Korea in 2014 and 2015. The monoene was not attractive at all at three places during the two years. In 2014, diene was equally or more attractive than the mixture in Jinju and Suncheon, respectively. In 2015 too, the attractiveness of diene and mixture was not different in Jinju and Munsan. Monitoring of the seasonal occurrence of E. batangesis with the sex pheromone components revealed that it occurred three times a year; the first occurrence from early April to mid May, the second from early Jun to mid July, and the third from late August to late September.

Non-target predatory insects can be indirectly exposed to aerial pesticide spraying and fogging to control Monochmus beetles that transmit pine wood nematode, Bursaphelenchus xylophilus. We evaluated potential lethal or sublethal effects of thiacloprid on survival and behavior of a carpenter ant species, Camponotus japonicus Mayr. Field-collected ant colonies were directly exposed to several food items, such as thiacloprid-addicted Monochmus beetles, 10% sugar watered cotton balls contaminated by thiacloprid concentrations, and 10% sugar water. Dead beetle bodies caused no apparent adverse effect through dietary exposure in general, although a few ants were died with paralysis at colony level experiment. At individual level, most ant workers were died within 10 days compared to control group. In contrast, dietary exposure of ants to thiacloprid concentrations showed significant lethal effect with paralysis and impaired walking, especially at 10 and 50 mg/L thiacloprid concentrations. Some intoxicated ants recovered within a few days in 10 and 50 mg/L thiacloprid concentrations, but intoxicated ants were generally shown to be less responsible to enemy ants with low aggressive behavior. Implications for predicting hazards of thiacloprid to beneficial arthropods in pine forests are discussed.

The nematode Bursaphelenchus xylophilus is the causal pathogen of pine wilt disease in Korea. Currently, it is reported that B. mucronatus also has a low pathogenicity. Despite this difference in pathogenicity, it is very difficult to differentiate these two species due to similarity in morphological characters. The sequence variation of internal transcribed spacer (ITS) and intergenic spacer (IGS) regions of ribosomal DNA has been used for species identification and phylogeny of B. xylophilus and B. mucronatus. But, the IGS region sequence data of B. mucronatus has been only reported in Portugal. In this study, We analyzed genetic variation on ITS and IGS regions of B. xylophilus and B. mucronatus (Asian genotype, European genotype) based on rDNA gene sequences, and conducted phylogeography using TCS network. When the each isolates was determined the phylogenetic analysis using the neighbor-joining method, Bursaphelenchus species were divided into each groups, and showed low variation within each species. In the TCS analysis, The isolate of B. xylophilus and B. mucronatus (Asian genotype and European genotype) were divided into each groups and confirmed slightly genetic distance within species. B. mucronatus European genotype has possibility of new species different with Asian genotype.

Insect antennae play important roles in finding mates and in locating food source and oviposition sites. Riptortus pedestris is an important pest of soybean and sweet persimmon in Korea. The male R. pedestris adult produce the aggregation pheromone attracting the conspecific nymphs and both sexes of adults. The pheromone was known as a cue for food finding, but the 1st instar nymph can develop to the 2nd instar without food. This phenomenon may suggest that the 1st instar nymph may have different sensilla system from other instars. Thus, we investigated the morphology and distribution of antennal sensilla and antennal response to the aggregation pheromone (AG) of each nymphal stage using Scanning Electron Microscope (SEM) and Electroantennography (EAG). As expected, first instar nymph did not have sensilla trichodea 3 (T3) and chaetica 3 (Ch3) which existed in other instar nymphs. The antennae of the 1st instar nymph did not responded to AG, with no difference from control. For further elucidation of the functions of sensilla T3 and CH3, single sensillum recording to AG will be done.

Bee venom contains a variety of peptides and enzymes, including serine proteases. While the presence of serine proteases in bee venom has been demonstrated, the role of these proteins in bee venom has not been elucidated. Furthermore, there is currently no information available regarding the melanization response or the fibrin(ogen)olytic activity of bee venom serine protease, and the molecular mechanism of its action remains unknown. Here we show that bee venom serine protease (Bi-VSP) is a multifunctional enzyme. In insects, Bi-VSP acts as an arthropod prophenoloxidase (proPO)-activating factor (PPAF), thereby triggering the phenoloxidase (PO) cascade. Bi-VSP injected through the stinger induces a lethal melanization response in target insects by modulating the innate immune response. In mammals, Bi-VSP acts similarly to snake venom serine protease, which exhibits fibrin(ogen)olytic activity. Bi-VSP activates prothrombin and directly degrades fibrinogen into fibrin degradation products, defining roles forBi-VSP as a prothrombin activator, a thrombin-like protease, and a plasmin-like protease. These findings provide a novel view of the mechanism of bee venom in which the bee venom serine protease kills target insects via a melanization strategy and exhibits fibrin(ogen)olytic activity.

Backgrounds : The action mechanisms of several chemopreventive agents derived from herbal medicine and edible plants have become attractive issues in cancer research. Tea is the most widely consumed beverage worldwide. It have been demonstrated that the active principles of tea sources such as flower extract Camellia sinensis (CSF) and Camellia japonica (CJF)were attributed to their tea polyphenols. We focused on investigating CSF, CJF, mixtures of CSF and CJF has been proven to suppress colonic tumorigenesis. Methods and Results : In this study, human colorectal carcinoma HT-29 cells were treated with CSF, CJF, mixture of CSF and CJF to examine the anti-proliferative and pro-apoptotic effects of mixture of CSF and CJF (3 : 1), as well as the molecular mechanism underlying these effects. Cell viability assay, nuclear staining, DNA fragmentation, caspase assay, cytochrome c release, were utilized to dissect the signaling pathways. In mixture of CSF and CJF (3 : 1), CSF appeared most anticancer effect by both MTT assays and the cleavage analysis of apoptosis-related molecules and PARP. Interestingly, we found that CJF make it possible to express the apotosis inducing by CSF in a short time and apoptosis effect of CSF maintained sustainable. Conclusion : In summary, our results from this study suggest that in HT-29 human colon cancer cells (i) CSF treatment causes damage to mitochondria, and (ii) CJF contributed CSF induced apoptotic cell death mediates cytochrome C release, (ⅲ) mixture of CSF and CJF (3 : 1) the potential to function as a chemopreventive agent against colon cancer.

Backgrounds : Camellia sinensis is known to have a very high antioxidant activity, but its petals are small and it is difficult to use it because of its low yield. In comparison Camellia japonica has many petals and yield, however, the biological effects of C. japonica have been less frequently studied. In the present study, we focused on investigating the in vitro antioxidant effect of the ethanol extract from flower of C. sinensis (CSF), C. japonica (CJF) and mixture of CSF and CJF. Methods and Results : Content of total phenolics and total flavonid, DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activities, reducing power, superoxide anion and hydroxy radical scavenging activity of CSF and CJF were compared in vitro experimetal models. Total phenolics was contained the higer in CJF (172.19±1.65 mgCAE/gEX) than 146.75±0.15 mgCAE/gEX in CSF. And effects of antioxidant measured by reducing power, superoxide anion generated by xanthine/xanthine oxidase and hydroxyl radical generated by the Fenton reaction (FeSO4 + H2O2) in a cell-free system was shown higher in mixture of CSF and CJF than BHT, ascorbic acid as a chemical oxidant which was detected by electron spin resonance spectrometry coupled with DMPO spin trapping. These results suggest that Camellia flower extract such as CSF and CJF exhibits antioxidant properties by scavenging ROS. Camellia extract contained quercetin, quercetin-3-O-glucoside, quercitrin and kaempferol, which are antioxidant compounds. Conclusion : As a result, the combination of CSF and CJF showed higher antioxidative effect than using CSF or CJF alone.

Background : Perilla frutescens L. is valuable as a medicinal plant as well as a natural medicine and functional food. Limonene perilla collected from various places showed 60% limonene compounds. However biological activity of these accession has not been reported before. Therefore, this study was conducted to investigate the biological activity of limonene perilla. Methods and Results : Fractional solvent extracts were obtained by using organic solvents such as n-hexane, chloroform, ethyl acetate, n-BuOH, and aqueous solvent from different parts of limonene perilla extracted initially in 70% EtOH. We investigated the effects of limonene perilla on total phenol and flavonoid contents, FRAP (Ferric Reducing Antioxidant Power), total saponin contents and tyrosinase inhibition activity. Leaves of limonene perilla produced the highest total phenolic contents (29.88 mg·CAE/g), flavonoid (8.39 mg·QE/g) and saponin contents (47.77 mg·GIE/g) than stems and roots of limonene perilla. FRAP of leaves was 823.00±3.58 μM·FeSO4·E/mg. Tyrosinase inhibition activity rate was 40.31% in 70% ethanol extracts from leaves of limonene perilla. Conclusion : This results suggest that leaf of limonene perilla fractions has significant antioxidant activity. Also, limonene perilla could be used as a functional biomaterial in developing cosmetics and functional foods.

Background : Plants are the rich source of antioxidants, which plays a very important role in maintaining human health. Their antioxidant property protects cells of different organs of human beings against free radicals and free radical mediated diseases. Even though, there is lack of knowledge on the antioxidant effect of lutein present in plants. In the present study, lutein was isolated from the GreenTea leaves (Camellia sinensis) which is used as a dietary source. Methods and Results : The procedure adopted for the isolation and purification of lutein using acetone extraction and preparative high performance liquid chromatography (HPLC) is simple and less time consuming. Free radicals scavenging activity of isolated lutein from acetone extract of GreenTea was assessed by DPPH radical scavenging assay and reducing power. The isolated lutein scavenged 79% of DPPH radicals at 20 ㎍/㎖ and two fold lower concentration compared to the standard antioxidants (α-tocopherol). No significant differences were found between the reducing power of the lutein and BHT when their concentrations were high. However, significant differences were observed at relatively low concentrations, the reducing power of lutein was isolated from the GreenTea leaves was stronger than those of their acetone extract and standard antioxidants (BHA). Both electron spin resonance (ESR) and in vitro assay confirmed that lutein was isolated from the GreenTea leaves, exhibited a greater capacity for scavenging superoxide (O2 •－) and hydroxyl (OH •) radicals than standard antioxidants β-carotene and α-tocopherol respectively. Conclusion : The results proven that lutein isolated from GreenTea leaves has an efficient antioxidant ability, it could serve as an antioxidant to scavenge reactive oxygen species.

Background : Lutein, a xanthophyll, consists of chains with 8 conjugated double bounds containing closed rings on each end of the chain. This carotenoid is found in fruits and vegetables, especially dark green leafy vegetables such as green tea. In this study, we investigated the anticancer effects of purified lutein from green tea on human cancer cell lines containing prostate carcinoma cancer cells (LNCaP). Methods and Results : Prostate carcinoma cancer cells (LNCaP) were cultured and evaluated the inhibitory effect of lutein isolated from green tea compared other carotenoids (β-carotene and lycopene) on cell proliferation. Cyclin D1 and PCNA were evaluated as cell differentiation. In results, PCNA/cyclin regulates the initiation of cell proliferation by mediating DNA polymerase. Under cultural conditions, lycopene remarkably suppressed the PCNA expression prostate cancer cell line LNCaP in higher doses (20 μM - 100 μM) statistically. However, β-carotene and lutein presented the less inhibitory effects on PCNA expression. Determination of PCNA expression in control and treated cells demonstrates that lycopene did affect proliferation in LNCaP cancer cells in dose-dependent manner. However, β-carotene and lutein suppressed the cyclin D1 expression in dose-dependent manner but no in lycopene group. These results indicate that differ carotenoids presented the various suppressive ability of PCNA and cyclin D1 expression in cell proliferation. Conclusion : In conclusion, lutein suppressed the carcinogenesis of induced prostate cancer cell line by acting as a suppressor for inhibiting the expression of cyclin D.

These days, animal infectious disease such as AI(Avian Influenza) and FMD(Foot-and-Mouth Disease) breaks out, and increase the number of its occurrence. Offered in "An animal disease prevention" of Ministry of Agriculture and Forestry that to do killed caused livestock order to prevent diffuse pollution of the epidemic when an animal disease has happened. Accordingly, in Ministry of Agriculture and Forestry set up the "Emergency Action Guide", it suggests the emergency cation, disposal method(stamping out, incineration, burying method), disposal method of livestock excretions in the area of origin. Also, the department of the environment makes the livestock burial regional environmental research guidelines, it order to investigate the surround environment, water quality in monitoring well, environment management and so on. In korea, this was the first time confirmed that the foot-and-mouth disease occurred in 1934. The leachate buried underground of livestock is organic waste water and it has been arranged nutrient salts such as nitrogen, phosphorus. So, there is a possibility that can lead to a variety of environmental problems when it can result in river pollution, ground water pollution and soil pollution. Look at domestic and foreign research trend leachate behavior such as contamination analysis through physicochemical properties or physicochemical analysis of the leachate components have been identified and carried out. Because of the leak of the leachate, there is a possibility about the around soil pollution. Thus, we studied that investigation of soil texture around livestock burial site in chung-buk north area of korea. From the result, surface soil was loam and subsoil was clay loam soil in 35% of survey site. In the future, this result will be a important basis data for correlation of leachate leak and consequential soil texture.

Along the increases of incineration bottom ashes emitted from the municipal solid waste incinerator, the issues, such as increased treatment costs, environmental problems and lack of land area for incineration treatment facility have raised. Therefore, this study was performed to analyze the incineration bottom ash to seek how to recycle the resources. The particles of bottom ash discharged as municipal solid wastes are not even and composed of inorganic substances such as iron and non-metals; in this study, therefore, the bottom ash are used as the basic data for the purpose of resource recycle. In this study, the waste incineration bottom ash emitted from the incineration treatment facility located in city C were analyzed. About 100 tons of municipal solid waste are incinerated in this facility on a daily basis. The particle size, XRF, TGA and ICP were analyzed for bottom ash. A LA-950(Laser Scattering Particle Size Analyzer) was used to perform a particle size analysis and as a result, the particle diameter of a large range was distributed and the particle diameter was shown to be wide so not evenly distributed. The distribution of particle diameter for each sample was shown to be inconsistent. XRF used an EDX-750 (Shimazu) to analyze the chemical components and as a result, the key components contained in the bottom ash included CO2, CaO, SiO2, Al2O3, B2O3, etc. The analysis revealed that CaO contained to be lower than other area. TGA / DSC 1 / 1600 LF(Mettler-Toledo AG) were used to analyze TGA and the heating rate of 10℃/min was applied up to the maximum temperature 1200℃. As a result, the sample of incineration bottom ash showed its significant reaction at around 700℃. In general, when temperature of bottom ash starts raising, the moisture started to evaporate at around 100℃ while a significant decline is observed in weight. However in this study, no significant change was observed around 100℃ followed by the pre-processed and bottom ash. ICP used 820 ICP-MS (Bruker, Germany) to analyze the heavy metal - As, Cd, Cr, Cu, Hg and Pb. 3 different bottom ash were divided into 3 samples and as a result, the average concentration of each substance was analyzed as As 0.0049ppm and Cu 0.006ppm, whereas the concentrations of Cd, Cr, Hg and Pb were observed to be less than the quantization limit; therefore, the concentrations of all 6 items were shown to be less than the hazardous level of the specified wastes.

The change of quality characteristics with storage temperature (room (25°C), low (15°C) and cold (4°C) temperature) and period of unhulled foxtail millet (Setaria italica Beauv. cv. Samdame and Gyeongkwan 1), proso millet (Panicum miliaceum L. cv. Hwanggeum-gijang and Manhongchal) and sorghum (Sorghum bicolor (L.) Moench cv. Hwanggeumchal and Donganme) were evaluated. The 1,000 grain weight, lightness, redness, yellowness and moisture content with storage temperature and period of unhulled foxtail millet, proso millet and sorghum were not showed difference. Germination percent and milling recovery of foxtail millet, proso millet and sorghum decreased with increasing storage temperature and period. Fat acidity of stored foxtail millet, proso millet and sorghum was increased with increasing storage period, and the higher temperature increased more. The results of this study show that the storage of foxtail millet, proso millet and sorghum at the low and cold temperature is better than the room temperature. Especially, in mind of the economics, the storage method is good low temperature than cold temperature.

Manganese () is a trace element that is essential for normal physiology, and is predominantly obtained from food. Several lines of evidence, however, demonstrated that overexposure to exerts serious neurotoxicity, immunotoxicity and developmental toxicity, particularly in male. The present study aimed to evaluate the effect of 0, 1.0, 3.3, and 10 mg/kg/day doses of on the reproductive organs in the immature female rats. Rats (PND 22; S.D. strain) were exposed to () dissolved in drinking water for 2 weeks. The animals were sacrificed on PND 35, then the tissues were immediately removed and weighed. Histological studies were performed using the uteri tissue samples. Serum LH and FSH levels were measured with the specific ELISA kits. Body weights of the experimental group animals were not significantly different from those of control group animals. However, ovarian tissue weights in 1 mg and 3.3 mg dose groups were significantly lower than those of control animals (p<0.05 and p<0.01, respectively). Uterine tissue weights of 3.3 mg dose groups were significantly lower than those of control animals (p<0.01), while the 1 mg dose and 10 mg dose failed to induce any change in uterine weight. Similarly, only 3.3 mg dose could induce the significant decrease in the oviduct weight compared to the control group (p<0.05). Non-reproductive tissues such as adrenal and kidney failed to respond to all doses of exposure. The uterine histology revealed that the exposure could affect the myometrial cell proliferation particularly in 3.3 mg dose and 10mg dose group. Serum FSH levels were significantly decreased in 1mg dose and 10 mg groups (p<0.05 and p<0.01, respectively). In contrast, treatment with 1 mg dose induced a significant increment of serum LH level (p<0.05). The present study demonstrated that exposure is capable of inducing abnormal development of reproductive tissues, at least to some extent, and altered gonadotropin secretions in immature female rats. Combined with the well-defined actions of this metal on GnRH and prolactin secretion, one can suggest the might be a potential environmental mediator which is involved in the female pubertal process.