After the major radioactivation structures (RPV, Core, SG, etc.) due to neutron irradiation from the nuclear fuel in the reactor are permanently shut down, numerous nuclides that emit alpha-rays, beta-rays, gamma-rays, etc. exist within the radioactive structures. In this study, nuclides were selected to evaluate the source term for worker exposure management (external exposure) at the time of decommissioning. The selection of nuclides was derived by sequentially considering the four steps. In the first stage, the classification of isotopes of major nuclides generated from the radiation of fission products, neutron-radiated products, coolant-induced corrosion products, and other impurities was considered as a step to select evaluation nuclides in major primary system structures. As a second step, in order to select the major radionuclides to be considered at the time of decommissioning, it is necessary to select the nuclides considering their half-life. Considering this, nuclides that were less than 5 years after permanent suspension were excluded. As a third step, since the purpose of reducing worker exposure during decommissioning is significant, nuclides that emit gamma rays when decaying were selected. As a final step, it is a material made by radiation from the fuel rod of the reactor and is often a fission product found in the event of a Severe accident at a nuclear power plant, and is excluded from the nuclide for evaluation at the time of decommissioning is excluded. The final selected Co-60 is a nuclide that emits high-energy gamma rays and was classified as a major nuclide that affects the reduction of radiation exposure to decommissioning workers. In the future, based on the nuclide selection results derived from this study, we plan to study the evaluation of worker radiation exposure from crud to decommissioning workers by deriving evaluation results of crud and radioactive source terms within the reactor core.

Customer management based on Customer lifetime value has emerged as the most effective way of doing business due to the ability to foster profitable CRM (Verhoef & Lemon, 2013; Villanueva & Hanssens, 2007). Although there has been much conceptual evidence of the positive link between customer equity and firm’s performance(Blattberg, Malthouse, and Neslin 2009), comparison of relationships between two competing firms based on customer equity and firm profitability is limited. Therefore, this research examines the role of customer equity on the firm profitability by comparing the company type such as leader and follower in customer equity setting. The result shows that the effect of newly acquired Customer equity of the second tier company is stronger than that of the top tier company while the effect of retained customer equity of the top company is stronger than that of the second tier company. Overall, the results provide strategic implications for firms to use a different customer equity strategy in a competitive market structure.

It is becoming increasingly essential for firms to achieve social improvement. Consumers no longer support firms that solely seek profits. A worldwide consumer survey found that 66% of respondents are willing to pay premium prices for brands from firms concerned with social well-being (Nielsen Report, 2015). Therefore, many firms actively engage in various societal marketing activities to elicit positive responses from consumers. As societal marketing continues to increase in importance, many studies have focused on the effect of corporate social responsibility activities. As one such activity, cause-related marketing (CM) is defined as “the process of formulating and implementing marketing activities that are characterized by an offer from the firm to contribute a specified amount to a designated cause when customers engage in revenue-providing exchanges that satisfy organizational and individual objectives” (Varadarajan & Menon, 1988, p.60). This enables a firm to improve its brand image or reputation and increase sales (Henderson & Arora 2010; Pracejus & Olsen 2004). A firm that employs CM can differentiate itself in competitive markets, which leads to increase in consumer’ purchase intention and their willingness to pay more for the firm’s brands (Müller, Fries, & Gedenk, 2014; Strahilevitz, 1999; Winterich & Barone, 2011). Therefore, CM expenditures are predicted to reach 2.06 billion dollars in 2017 (IEG Report, 2016). Further, CM growth continues worldwide in both developed and developing countries (Adkins, 2008; Wymer & Samu, 2009). Therefore, we focus on CM among various societal marketing activities. Many firms conduct CM in markets that differ both economically and culturally. However, cross-country or cultural research on consumers’ responses to CM is scarce. Although some studies consider cultural factors, most are based on Western-Eastern or individualism-collectivism typology (Ralston, et al., 2008; Yuan, Song, & Kim, 2011). It is dangerous to assume a bi-cultural continuum without considering the multiple variations within the Western or Eastern worlds. The current research addresses this limitation in literature, by exploring CM within the Asian market. We specifically examine Korea and China, as these countries are included in Asian culture, but differences exist between the two. The majority of previous research has discovered factors for successful CM, including the donation magnitude and quantifier, product traits (e.g., type, involvement), the company’s characteristics such as reputation or image, and the cause’s familiarity or importance (Barone, Miyazaki & Taylor, 2000; Lafferty, & Edmondson, 2014; Pracejus, Olsen, & Brown, 2003; Samu & Wymer, 2009). The results regarding the product type’s effect on consumer responses toward CM, revealed that guilt received considerable attention. It has been found that guilt generated by hedonic consumption compels consumers to purchase CM products. Further, the consumer regards the product’s CM as a means of justifying their hedonic product purchase. Many studies demonstrate that consumers choose CM products to reduce guilty-feeling regarding consumers simply as beings who seek to maximize utility. However, CM must also be illuminated, as CM is marketing strategy that involves donation. In summary, we take both economic and ethical perspective based on the dual process theory. From an economic view point, we examine the effect of guilt on consumers’ preferences for CM products. We then propose a new factor from an ethical perspective. Further, we study the two factors’ impacts on consumers’ decision-making through a cultural comparison. We conducted a between-subjects experiment. It was found that Korean consumers felt guiltier and the mean of perceived value of CM was higher in Chinese consumers (Table Ⅰ). As the below Figure Ⅰ illustrates, there was significant interaction effect between countries (Korea versus China) and product type (utilitarian versus hedonic). We also confirmed that the underlying consumer response mechanism to CM products differs between Korean and Chinese. These results support prior studies’ conclusions, in that guilt is the key factor in consumers’ decision-making processes regarding hedonic CM products. And we present the value perception as new influential factor. This paper provides several implications. First, we attempt to overcome previous research’s narrow viewpoint toward CM. As research is scarce regarding the ethical or philanthropic aspect of CM, we focus on that aspect with a particular focus on consumers’ perception of CM value. Second, this paper draws on the dual mode theory, to proposes another new influential factor that affects consumer behavior. As dual processes’ relative effects may differ depending on context (Sonenshein, 2007), therefore, and third, this study examines these effects using a cultural context. Korea and China have a substantial ripple effect on the global economy, thus, it is valuable to study the two countries’ different responses toward CM. Finally, our study provides more insight and practical implications for firms in the Korean and Chinese markets.

Firms acquire customers using myriad forms of marketing media (Neslin & Shankar 2009), and different media strategies yield different results to the firms. Therefore, allocating media strategy given a firm’s spending raises important questions for managers. This is especially the case since the media landscape has changed dramatically, with new media channels incorporating online, mobile, and social media now being considered the mainstream. It is crucial to understand how each form of media influences consumers and how it operates alongside traditional media. Based on Stephen and Galak (2012), marketers distinguish earned media from paid media. Earned media is defined as media activity that a company does not directly generate, such as press mentions on the internet and online community posts in consumer-generated social media. On the other hand, paid media refers to the media activity which a company generates (for example, television, radio, print, and direct mail). It is common for firms to consider using earned media and paid media at the same time when developing marketing communication strategies. Despite the coexistence of paid and earned media channels, previous empirical findings focus either on paid media or earned media and suggest that these individually will increase a firm’s marketing outcomes. However, there is a lack of research that examines the question of whether the use of paid media and earned media at the same time is synergistic. The effects of a cross media synergy only focuses on the resource allocation within paid media (for example, TV–Radio (Edell & Keller 1989), TV–Magazine (Confer & McGlathery 1991), TV–Print (Dijkstra, Buijtels, & Van Raaij 2005), and TV, Radio, Print and Outdoor (Briggs, Krishnan, & Sheeran 2003)). Thus, by considering paid media and earned media concurrently, this study investigates whether the synergies between paid and earned media have a stronger effect on a firm’s long term profitability than the isolated effects of TV or word of mouth (WOM) alone. In addition, the research on earned media has focused on short-term outcomes such as customer actions (for example, website sign-ups) and sales growth, sales rank, cross-product sales, and ratings (Trusov, Bucklin, & Pauwels 2009; Li & Hitt 2008; Moe & Trusov 2011). Moreover, in the limited research on the relationship between earned media and long-term outcomes, the outcomes are restricted to those related to soft metrics of communication effectiveness (for example, attitude and brand awareness). Therefore, we use customer equity, which is regarded as a forward looking firm outcome variable, thereby enabling marketers to monitor and measure the long-term financial impact of marketing spending (Kumar & Shah 2015). Moreover, cross-media synergy can be accurately measured by customer equity, which incorporates both customer acquisition and retention. Based on Villanueva, Yoo, and Hassens (2008), customers acquired through paid media focus more on trials, whereas customers acquired through earned media provide the firm with more repeats. In other words, paid media plays a key role in the acquisition of customers, while, on the other hand, earned media increase the retention of customers. Thus, it is appropriate to measure the cross-media synergy with the customer equity (long term profitability) that can capture the customer acquisition and retention simultaneously. Regarding the long term impact of the firm’s media strategy, previous research has used the economic impact of traditional marketing channel (for example, television, radio, magazine or newspaper, advertisement, e-mail links, and direct mail) versus that of WOM (for example, links from Web sites, magazine, or newspaper articles, referrals from friends or colleagues, referrals from professional organizations or associations, and referrals from search engines) on customer equity. Traditional marketing had a stronger effect than WOM in the short term, while WOM is a quiet, gradual-impact, long lasting driver (Villanueva, Yoo, & Hassens 2008). This result can be attributed to the different characteristics of each media channel. Although earned media, including WOM, is not entirely controlled by the firm, earned media may be more likely to last longer for various reasons. One of the reasons for this phenomenon is that earned media has greater credibility than conventional marketing activities that are implemented by the firms, and is therefore more persuasive than conventional advertising (Brown & Reingen 1987, Villanueva, Yoo, & Hassens 2008). In other words, considering the impact of each type of media in itself, earned media is more effective in increasing long-term profitability. However, the interaction effect of earned and paid media has not been empirically tested yet. Thus, it is conceivable that a cross-media synergy (incorporating the implementation of earned media and paid media at the same time) will last longer than the implementation of each isolated media. As Armelini and Villanueva (2010) pointed out, earned media and paid media have complementary effects. For example, offline advertising increases website visitation by influencing consumer awareness, while online advertising directly leads to website traffic (Ilfeld & Winer 2002). The consumer buying process involves distinct stages such as awareness, consideration, and purchase (Lavidge & Stener 2000) and each media influences customer buying behavior in a different way. Hence, it enhances the effectiveness in terms of long-term profitability to utilize the cross-media effect properly. For example, in the car industry, 64 % new car buyers become aware of the features and benefits of a car by obtaining information online, even though they purchase their cars from an offline dealership (J.D. Power and Associates 2004). This finding implies that a firm’s implementation of both paid and earned media properly will maximize the customers’ arousal of the target brands. Furthermore, converging paid media and earned media is expected to proliferate the growth of a firm’s profitability, such as sales, revenue, and customer’s equity, at an exponential rate. For example, the effects of TV advertising execution can be enhanced by press mentions that a company does not directly generate; this is because press mentions support the credence of TV advertising. Inversely, since paid media activities reach the audience relatively more than WOM (due to the high audience penetration share), the online share of press mention can proliferate rapidly with the execution of paid media activities. Therefore, the interaction between earned media and paid media has a greater effect on customer equity than isolated media implementation. The impact of a media synergy has more positive effect and last longer than isolated media implementation (and our model is displayed in Fig. 1). We collect data on marketing efforts, word-of-mouth circumstance, and performance of a telecommunication company. Based on customer equity models and quarterly marketing and performance data, we first estimate the lifetime value of the newly acquired and existing customers. Thereafter, we determine the customer equity of the company over each period. We develop and employ a time-series model for examining the relationship between cross media efforts (paid media vs. earned media) and the estimated firm’s customer equity. Finally, we examine the synergistic effect of cross media on the firm’s long-term profitability.

Cross-buying refers to the customer action of buying additional products and/or services from the same provider (Valentin 2004). With the belief that cross-buying enables firms to increase profit from existing customers, firms have steadily placed greater emphasis on cross-selling strategies for profitability. To date, numerous studies show that cross-buying behavior of customers has a positive effect on firm profitability. Business reality, however, offers a different perspective; namely, that high levels of cross-buying may not always be linked to firm profitability. For example, Best Buy (an electronics retailer in the United States) has identified approximately 20% of its customers as unprofitable in spite of them purchasing multiple items (McWilliams 2004). Shah, Kumar, Qu, and Chen (2012) found that customers who persistently exhibit certain types of behavior (e.g., excessive service requests, high levels of returning products, lower levels of revenue growth, promotion maximizers) are unprofitable even though they purchased more than one product category.The aforementioned research implies that cross-buying can exert a negative impact on profitability, thereby calling for further examination of cross-buying behavior. It is conceivable that a repeated purchase propensity (contrasted with a cross-buying propensity) concentrated on a single brand is more profitable. Therefore, our primary objective in this paper is to identify a more beneficial type of customer among those who tend to patronize a limited number of brands versus those who tend to patronize a variety of brands, using a one-dimensional model (brand dispersion index). In addition, the second goal of this research is to investigate the boundary conditions where cross-buying will not lead to an increase in sales (unprofitable cross-buying conditions). As two moderating factors that weaken a customer’s crossbuying propensity and a firm’s sales (frequency and transaction size of firms), we consider (1) promotion dependency and (2) spending limiter condition. We use transaction data that include partners in various industries such as gasoline stations, convenience stores, banks, restaurants, and online shopping malls, covering forty-seven categories. Because multiple partners in many categories are available, this allows us to study whether a customer’s cross-buying level in the current period (t) affects the customer’s purchase frequency and transaction size in the subsequent period (t+1). The observation period for the data set extends over three years. Findings from this study indicate that a high level of cross-buying at period t has a positive impact on increasing customer frequencies and transaction sizes in the subsequent (t+1) period. This means that cross-buying has the potential to increase the firm’s profitability. Customers who show a high level of cross-buying propensity tend to exhibit higher levels of loyalty than customers who concentrated on limited brands. Firms should find ways to induce customers with low cross-buying propensity to increase crossbuying. Regarding moderating effects, promotion dependency and spending growth (decline vs. stagnation), spending growth has a considerable moderating effect on the relationship between cross-buying propensity and a customer’s transaction size. Specifically, the effect of cross-buying on transaction size weakens when spending is shrinking. This result makes an important contribution to cross-buying research. If customers showing a high level of cross-buying do not increase their spending level, they may be merely switching to other brands in the program under a fixed budget. So while the rate of crossbuying seems to increase, profit might not increase. The findings from this study imply that it is crucial to target and motivate customers who tend to use various brands and contribute to sales to do more cross-buying instead of suggesting cross-buying to random customers. The promotion dependency, however, turns out to not have significant moderating effects on the relationship between the customer’s propensity to cross-buying and the customer’s purchase frequency and transaction size. For marketing purposes, it is important to consider which customers are more profitable among those who tend to do cross-buying among multi-brands versus those who tend to purchase repeatedly in a limited number of brands. This research provides a solution with a one-dimensional index, the brand dispersion index. Whether cross-buying is shown to be a positive or negative impact on sales, the results are meaningful in implementing customer relationship management. Regardless of the direction in the level of crossbuying, both directions provide a solution to allocate marketing resources. For instance, if the propensity for cross-buying increases sales, the firm should implement marketing strategies to encourage people to use a variety of brands by adding new brands. If repeat purchases increase sales, the company should concentrate on certain brands that customers use most frequently. In addition, by finding the conditions that do not increase sales (e.g., spending limiter condition), it makes marketing practitioners think that cross-buying does not always bring positive results. Overall, the findings from this study are that it is crucial to motivate and target customers who tend to use various brands and contribute to sales to do crossbuying activity, instead of promoting cross-buying to random customers. Conceptual Framework Figure 1 provides an overview of our framework for the relationship between brand dispersion and visiting frequency and transaction size of customers. Specifically, we hypothesize how customer frequencies and transaction sizes in time t+1 will be influenced by customer brand dispersion levels (the extent that customer transactions occur across a broad range of brands) in time t. In addition, we examine the moderating influence of two customer specific variables: (1) degree of promotion dependency and (2) spending limits.

Although there has been much conceptual evidence of the positive link between customer equity and firm’s performance, comparison of relationships between two firms based on customer equity and firm profitability is limited. However, there is an increasing demand for research that investigates the relationship between firms in the competitive market structure. Therefore, the goal of this research is to examine the role of customer equity on firm profitability by comparing two firms, in a customer equity setting. The result shows that the effect of newly acquired CE of the second top company is stronger than that of the top company while the effect of retained CE of the top company is stronger than that of the second top company. Overall, the results offer strategic implications for firms to implement a different customer equity strategy in this competitive market structure.

Consumers who play a key role in the Marketing 3.0 era tend to prefer socially responsible firms to profit-seeking firms. Hence, when creating a vision statement or setting a goal, firms make an effort to project an image of someone who cares for human and societal values. When managing relationships with consumers, many firms actively engage in various societal marketing activities and try to elicit positive responses from consumers. An example of such an activity is cause-related marketing (CRM), “the process of formulating and implementing marketing activities that are characterized by an offer from the firm to contribute a specified amount to a designated cause when customers engage in revenue-providing exchanges that satisfy organizational and individual objectives” (as cited in Varadarajan & Menon, 1988, p. 60). CRM positively influences consumers’ attitudes and purchase behavior (Olsen, Pracejus, Brown, 2003; Pracejus & Olsen, 2004; Henderson & Arora, 2010), and has become a strategic tool for increasing firm value and improving financial performance in the long term (Dean, 2004). As a result, investment in CRM in the U.S. is rapidly increasing, with annual average growth of more than 12%. In Korea, CRM came into the spotlight after the mid-1990s. In China, a successful implementation by Nongfu Spring in 2006 attracted more interest and more investment in CRM. As firms worldwide increasingly employ CRM, researchers have become more interested in CRM as well. Most studies focused on finding factors that affect the effectiveness of CRM. However, most studies were conducted in the West, so the strategies or guidelines they offer are not directly applicable in other cultural contexts. A successful implementation of any marketing tool, including CRM, requires understanding its cultural context. Understanding culture is essential for effective marketing, for entering and growing in foreign markets (Steenkamp, 2001; Mueller, 2004). In this paper, we study the effect of CRM, taking into account cultural factors. We focus on Korea and China. We chose these two countries because they are both part of the larger Asian market, yet have different cultural characteristics. Our study thus extends prior research that was mainly conducted in the West. We examined Chinese consumer responses to CRM. CRM is not a common concept in China, but Chinese consumers demand that both national and foreign companies offer socially responsible products and services. Despite the recent slowdown, China is still a market with a big potential, attractive to multinational corporations. Hence, our study carries practical significance. This comparative study may be especially useful to Korean firms, as they show the highest rate of entering the Chinese market. We tackle several issues in this paper. First, we examined the responses of both Korean and Chinese consumers to CRM from a comparative perspective. Second, we analyzed the moderating effect of product type on consumer response to CRM. Product type has been treated—with contradictory results—as an important variable in previous research (Strahilevits & Myers, 1998; Burnett & Lunsford, 1994; Winterich & Barone, 2011). Our study provides more insight into those results, and suggests what the practical implications are for firms in the Korean and/or the Chinese market. Third, we explored the response of Chinese consumers to CRM by regional groups. The Chinese economic reform of the early 1990s allowed for many different lifestyles to emerge in China (Wei, 1997). There are regional differences also in economic, social, and cultural aspects, consumer characteristics, or consumer behavior (Schmitt, 1997; Cui & Liu, 2000; Sun & Wu, 2004). Rather than treating China as one single market, segmenting markets becomes a prerequisite for effective marketing in China. Building on results and insights from previous research and taking account of differential consumer responses, we found that there are regional differences in how Chinese consumers react to CRM.

Although several causal studies investigate the relationships between customer equity (CE) and firm performance, there has been some debate about whether their positive relationship is valid over long time horizons and across firm/industry environments. In this paper, we investigate the dynamic effect of CE on firm performance. Using individual-level purchase data for an online retailer, we find a weak relationship between CE and firm profitability, which is not consistent with previous assumptions and beliefs. Additional analysis to resolve this gap shows that in the early stage when a firm’s growth rate is relatively high the firm is required to manage many newly enrolled customers. This means that newly acquired customer equity (NCE) has a larger effect than retained customer equity (RCE) on firm profitability in the early stage. In contrast, in the mature stage when a firm’s growth rate is stable and low the firm should retain its customers. This means that RCE has a larger effect than NCE on firm profitability in the mature stage. Thus, marketing managers need to leverage the drivers of acquisition and retention to continue to grow overall CE and firm performance.

Although the role of Guanxi in the Chinese business to business (B2B) market as a form of relationship marketing has received increasing attention in recent years, few empirical studies have explicitly distinguished between Guanxi and relationship marketing. Westerners typically consider Guanxi as unethical, but foreign-invested enterprises (FIEs) may have some difficulty in fully practicing relationship marketing in China without considering the influence of Chinese culture. In this regard, this study is guided by the following research question: “In China, should foreign-invested enterprises (FIEs) adopt Guanxi instead of relationship marketing in the B2B market?” In this study, we first provide an overview of previous research on Guanxi, focusing on the fundamental differences between Guanxi and relationship marketing. We then provide an empirical analysis of the differential effects of Guanxi and relationship marketing on firm performance by investigating 295 FIEs in the Chinese B2B market. The results suggest that Guanxi and relationship marketing are not trade-off options in today’s Chinese market. Guanxi and relationship marketing have synergetic effects on firm performance, that is, they have differential effects based on the mode of market entry and the type of competitor. Guanxi is more likely to influence firm performance for collaboration based entry firms rather than entry without collaboration firms, whereas relationship marketing is more likely to influence firm performance when FIEs’ main competitors are foreign firms than when they are local firms.

Artificial insemination (AI) has been performed widely in swine industry using fresh liquid sperm instead of frozen type of sperm. However fresh sperm are not able to preserve more than three days with optimal motility and other sperm parameters for the successful fertilization, since in vitro stored sperm has an oxidative stress that resulted increase of abnormality and acrosome reation. To overcome these major problems, novel preservative formulation is needed to neutralize the oxidative stress and to provide suitable physiological environment for sperm in in vitro. In this study, naturally derived substances such as Poncirus trifoliate (Trifoliate orange), Garcinia mangostana (Mangosteen), pig placenta and testis extracts were tested as sperm preservative agents. Placenta extracts (PE), trifoliate orange extracts (TOE), testes extracts (TE) and mangosteen extracts (ME) were applied to analyze specific parameters for sperm motion characteristics individually and combinatorial. Each individual extract treatment can accelerate the sperm motility but noticeably TOE, TE and ME treatments exhibited the considerable and significant preservation of sperm motility. PE, TE and ME showed a significant (p<0.05) increase in ALH after one week. Further we evaluated the five different combinations of these extracts on sperm motility and its motion characteristics. Surprisingly even after one week ME, TOE and TE combination significantly preserved the sperm motility about 75%. It is noteworthy that unlike individual extract treatment, combination of ME, TOE and TE simultaneously protect the sperm motility and its motion characteristics. Taken together these data conclude that addition of ME, TOE and TE can be effective for preservation of pig sperm.

Althogh Spermatogonial stem cells (SSCs) are widely studied in mice, study of pig SSCs is not sufficient for the isolation, long-term culture, and characterization. To identify the effect of growth factor in cultured pig SSC, newly generated pSSCs like cell from neonatal 5days porcine testis were cultured and investigated for the pSSCs like cell formation. Glial derived neurotrophic gactor (GDNF), fibroblast growth factor (FGF), leukemia inhibitory factor (LIF), and epidermal growth factor (EGF) were applied to culture media to identify the pSSC like cell growth and stem cell formation. The criteria for the determining of stem cell characters, morphology, number of colonies, putative stem cell marker were analysed by microspic, polymerase chain reaction (PCR) and immunocytochemistry (ICC) methods. Most of the pSSCs like cells were formed approximately 100 μm size with sphere shape. Most of the feeder cells were highly dependent on FGF that feeder cells were not stably attached on plate without FGF and colony formation of pSSC was not observed consequently. Immunocyto chemistry data revealed that this cells expressed the ubiquitin-C-terminal hydrolase 1 (UCHL-1, PGP9.5) and Dolichos Biflorus Agglutinin (DBA) in addition of 20 ng/ml EGF, 10 ng/ml FGF, 10 ng/ml GDNF, 10 U3/ml LIF. In addition, Alkaline Phosphatase ()was positive in all period of culture. This study suggest that various growth factorsinp SSC culture system is important to regulate and maintain the pSSC. In conculsion, although the precise role of growth factor in pSSC proliferation need to be clarified, combination of growth factor might be critical in order to derivation and proliferation of neonatal pSSCs and spermatogenesis.

INTRODUCTION In rodent, molecular markers of spermatogonia, spermatocyte, spermatid and sperm have been identified. It has been reported that DBA, PGP 9.5 and NanpG can be the markers for spermatogonia in pig. For further understanding the spermatogenesis of the pig on morphological and molecular level, we report identification of testicular cells in neonatal and pubertal pig testis, and a putative marker for spermatogonia and spermatid in pig testis. MATERIALS AND METHODS Neonatal (3 day) and pubertal testis (150 day) was cut and fixed in Bouin’s solution for immunohistochemistry (IHC). Gonocytes were isolated from neonatal testis for immunocytochemistry (ICC). Based on references (Phillips et al., 2010), thirteen antibodies (VASA, Oct4, NanoG, PGP 9.5, DAZL, SCF, GFR-alpha 1, PLZF, c-kit, integrin-beta1, Thy1, Sohlh1 and CD9) were used for IHC and ICC. Paraffin section was performed for IHC. Gonocytes were attached to the APS-coated slides for ICC. HRP-conjugated and florescent-labeled secondary antibody was used for IHC and ICC, respectively. RESULTS In histological analysis, spermatogonia and Sertoli cells, which are enclosed by seminiferous tubule and connective tissue, were observed in neonatal testis. However, complete spermiogenesis, including spermatocyte, spermatid and spermatozoa, was not observed in neonatal testis. Spermatocyte, spermatid and elongated spermatid were observed in pubertal testis but matured spermatozoa were not observed. As a result, two antibodies (PGP 9.5 and GFR-alpha1) of thirteen antibodies were available for IHC and ICC. As reported in other studies, PGP 9.5 protein was detected in spermatogonia of ne-onatal in IHC. In addition, it was observed in spermatogonia of pubertal testis. GFR- alpha1 protein was detected in spermatogonia of neonatal testis and spermatid of pubertal testis. In ICC, PGP 9.5 protein was detected in gonocytes as reported in other studies. GFR-alpha1 was also observed in gonocyte isolated from pig testis. In this study, we found that 1) only spermatogonia exist in neonatal pig testis (3 day), 2) GFR-alpha1 is a new marker for spermatogenesis in pig testis.

Interferon induced transmembrane protein-1 (IFITM1) is one of transmembrane protein which is differentially expressed in uterus during estrus cycle and pregnancy, that IFITM1 gene is highly expressed in estrus stage by the effect of estrogen, and in parturition by the effect of PGF2 alpha. This genes are also up-regulated in cells with hyperactivation of the WNT/β-catenin signaling pathway. In this study, to identify a function of IFITM1, the binding partner of IFITM1 were determined using immunoprecipitation and LC- MASSMASS methods. 1, 3 and 5 ug of polyclonal anti-IFITM1 antisera were used for immunoprecipitation, and the 75 kDa of specific band was detected in silver stained polyacylamide gel. This band were chracterized using LC-MASS-MASS, and revealed this band is glucose regulated protein 75 (GRP75) which binds to p53 and inhibits the p53 action in nucleus. To identify the localization of GRP75 in cells, immunocytochemical approach has been applied, and GRP75 is expressed in mitochondria of L929 murine connective tissue cells. Co-localization study between IFITM1 and GRP75 in L929 cell identified that these two proteins were closely expressed in mitochondria. Although the role of the interaction of these two protein need to be clarified in various biological phenomena, this data suggest that close interaction of IFITM1 and GRP75 may regulate cellular functions in uterus on sets of estrus cycle and pregnancy.

Extravasation is the accidental injection or leakage of fluid into the subcutaneous or perivascular tissues. Some drugs can cause serious injury such as severe tissue injury, necrosis, and etc. Here we report a case of chemical burn by sodium bicarbonate extravasation due to accidental venous puncture during arterial cannulation. A 42-years-old woman has taken emergency laparotomy surgery due to a stab wound to the abdomen. Massive blood loss has developed, and consequently vital signs were unstable and metabolic acidosis has developed. Sodium bicarbonate has administered via a peripheral intravenous line on the dorsal vein of a right hand that runs to the cephalic vein. However, the cephalic vein that runs by the side of the radial artery has punctured accidentally during the attempt of right radial artery cannulation. Second degree superficial and deep chemical burn by sodium bicarbonate extravasation has developed. Skin lesion about 3 × 4 cm2 with erythema and bullae formation has developed. There were no necrotic changes and the digital sensation was intact. Wet dressing and silicon foam dressing were prescribed. After two weeks, she was discharged. Until then, dermis exposure about 1 × 1 cm2 remained although the skin lesions became getting well.

Kwanganbyeo is a japonica rice variety developed from the cross between Namyang 7 and SR14779-HB234-31, anelite line derived from the cross between Cheonmabyeo and Aichi 37 at the National Crop Experiment Station, RDA, and washi-tecture and light-dark gre