목적: 입체경에서 기하학적 단안 단서를 타깃의 배경에 적용하여 거리감 지각의 변화를 확인하였으며, 가상현실에 서 거리감의 효과적인 구현 방법을 분석하였다. 방법 : 타깃의 배경에 사용된 단안 단서는 결의 밀도, 선형 전망, 대기 전망 및 음영이었다. 결의 밀도 단서 및 선형 전망 단서는 선의 개수와 대비도, 대기 전망 단서 선의 개수와 대비도 차이, 음영 단서는 음영의 농도와 범위 를 각각 변수로 선정하였다. 타깃은 모든 대상자에게 무작위로 제시하였으며, 원거리 및 근거리에서 검사 후 5점 척도의 설문조사를 통해 거리감 변화를 확인하였다. 또한, 거리감 변화에 대해 대상자의 지각이 의도한 것과 일치 했을 때 긍정적, 그렇지 못했을 때 부정적 효과로 구분하고, 거리감 변화의 효율을 평가하였다. 결과 : 원거리와 근거리 모두 단안 단서의 변수에 대해 의도한 것과 대부분 일치하는 것으로 확인되었으며, 근 거리는 일부 변수 조정 항목에서 다소 차이가 발생했다. 또한, 거리감 변화 효과는 결의 밀도 단서 및 대기 전망 단서에 의해 선의 개수가 변화했을 가장 높았고, 일부 유의하였다(p<0.050). 결론 : 입체경에서 대상자의 거리감은 주시거리가 동일해도 타깃의 배경에 적절한 단안 단서를 제공하여 조정 이 가능한 것으로 확인되었다. 원거리 및 근거리 입체시표의 기하학적 단서 중 결의 밀도 및 대기 전망 단서에서 선의 개수를 변화시켰을 때 거리감 지각 변화의 효과가 가장 높은 것으로 나타났다. 보다 정확한 거리감 지각을 위해서는 조절 및 폭주 변화 등을 추가한 후속 연구가 필요할 것으로 판단된다.

목적 : 입체경으로 구현된 가상공간에서 측정한 사위검사를 하웰 시표를 기반으로 하는 기존 검사와 비교하여 효용성을 알아보고자 하였다. 방법 : 전체 대상자는 51명(20~39세)으로 전신질환, 안질환, 억제가 없고, 입체시가 40 sec 이상, 나안 또는 교정시력이 양안 1.0 이상인 사람을 선정하였다. 입체경의 광학렌즈 굴절력은 +3.00 D, +5.00 D, +10.00 D이 고, 광학중심점 간 거리는 62.00 mm이었다. 하웰 시표를 기반으로, 기존과 입체경용 사위도 검사 시표를 제작하 였으며, 검사거리 500.00 cm, 40.00 cm, 33.00 cm에 대해 수평과 수직사위도 검사를 무작위로 수행하였으며, 계산 조절성폭주비를 구하였다. 결과 : 검사거리 500.00 cm, 40.00 cm 및 33.00 cm에서 측정한 기존, +3.00 D, +5.00 D, +10.00 D 입체경 에서 수평사위(△)는 각각 -1.18±1.71, +2.39±3.46, +2.23±3.36, +2.36±3.31(500.00 cm), -3.88±4.17, -4.92±5.92, -4.98±5.70, -4.91±5.58(40.00 cm), 및 -5.53±4.82, -6.50±6.67, -6.49±6.59, -6.38±6.54 (33.00 cm), 수직사위(△)는 각각 -0.03±0.97, -0.03±0.99, -0.03±0.87, +0.02±1.00(500.00 cm), 0.00±0.92, -0.05±1.00, -0.09±1.01, -0.02±1.02(40.00 cm), 및 -0.05±0.99, -0.01±1.00, -0.11±1.02, -0.05±0.99(33.00 cm)로 나타났다. 입체경의 원거리 수평사위에서 더 내사위, 근거리는 더 외사위 방향으로 나 타났으며, 원거리만 통계적으로 유의한 차이가 있었고(p<0.050), 수직사위는 유의한 차이가 없었다(p>0.050). 계 산 조절성폭주비(△/D)는 각각 5.18±1.24, 3.34±1.45, 3.38±1.37, 3.36±1.35(40.00 cm), 및 4.81±1.22, 3.30±1.41, 3.36±1.41, 3.35±1.38(33.00 cm)이었으며. 입체경에서 더 낮았으며, 유의한 차이가 있었다 (p<0.050). 결론 : 입체경을 이용한 사위도 검사는 구현된 가상공간에 대한 거리감 인지가 실제공간과 다르기 때문에 원거 리 과폭주, 근거리 저폭주의 영향이 발생되었다. 실제공간과 유사한 수준을 가지기 위해서는, 거리감 인지를 증가 를 위한 주변부 가상공간에 경험적 단서를 추가시킬 필요가 있다고 판단된다.

This study evaluated the antimicrobial activity of Endoseal TCS, an mineral trioxide aggregate-based root canal sealer, mixed with water-soluble mangostin derivatives (WsMD) of Garcinia mangostana L. (mangosteen) ethanol extract against Enterococcus faecalis and Staphylococcus aureus. The antibacterial activity of Endoseal TCS mixed with WsMD against three strains of E. faecalis and three strains of S. aureus was performed using agar diffusion test. The data showed that Endoseal TCS mixed with 0.115% WsMD had a zone of inhibition of 0.7 ± 0.2–2.4 ± 0.1 mm. The results suggest that Endoseal TCS mixed with WsMD of Garcinia mangostana L. ethanol extract is useful as a root canal sealer with antibacterial activity against E. faecalis and S. aureus .

Enterococcus faecalis is a major causative agent of endodontic treatment failure. The purpose of this study was to investigate bactericidal effects of ethanol extract of Garcinia mangostana L. (mangosteen extract) on five strains of E. faecalis that were isolated from human oral cavities. The bactericidal effects of mangosteen extract were assessed by measurement of minimum bactericidal concentration (MBC) value. The cytotoxicity of mangosteen extract on immortalized human gingival fibroblasts, hTERT-hNOF, was determined based on cell counting method. The data revealed the MBC value of mangosteen extract against the E. faecalis strains was 4 ㎍/ml. Additionally, the cell viability of mangosteen extract on hTERT-hNOF was 83.7-89.1% at the 1 to 16 ㎍/ml. These findings indicated that mangosteen extract could be used as a root canal cleaner during management of endodontic treatment failure caused by E. faecalis.

Polyphenon 60 refers to the mixture of catechins present in green tea. The aim of this study was to investigate the antimicrobial activities of polyphenon 60 against 4 strains of Streptococcus mutans and 2 strains of Streptococcus sorbrinus, which are the major causative bacteria of dental caries. The minimum bactericidal concentration (MBC) values of polyphenon 60 for S. mutans and S. sobrinus were determined and the effect of biofilm formation inhibition of that was evaluated. The MBC value of polyphenon 60 against the bacterial strains was 2.5 mg/ml except for one particular strain, S. mutans KCOM 1128 for which the value was 1.25 mg/ml. The results of biofilm formation inhibition assay revealed that polyphenon 60 inhibited biofilm formation more than 90% at a concentration of 2.5 mg/ml. It was apparent that polyphenon exhibited biofilm formation inhibition activity along with bactericidal effect against S. mutans and S. sobrinus. Therefore, it is proposed that polyphenon 60 as one of the components of bactericidal agents could be useful in developing oral hygiene products, toothpaste or gargling solution.

본 연구는 표고균사체 발효 적하수오 열수 최적 추출조건 탐색을 목적으로 수행하였으며, 표고균사로 배양된 하수오의 독립변수로는 독립변수(Xi)로서 추출온도(X1), 추출시간(X2) 및 시료에 대한 용매비(X3)에 대한 실험범위를 설정하여 각각을 5단계로 부호화하였고 중심합성계획에 따라 16구로 설정하여 추출하였다. 또한 이들 독립변수에 영향을 받을 종속변수(Yn)로는 고형분 함량(Y1) 및 당도(Y2), TPC (total polyphenol contents, Y3), TFC (total flavonoid contents, Y4), ABTS cation radical scavenging activity (Y5), DPPH radical scavenging activity (Y6)으로 하였다. 고용분 함량 최대값과 최소값은 각각 31.99%와 16.84%로 나타났다. 이를 회귀분석한 결과 고형분 함량에 대한 모델식의 R2은 0.867로 나타났다. 당도는 7번(8 hrs, 85℃, 67 mg/mL)과 15번(6 hrs, 70℃, 100 mg/mL)시험구에서 3.0 brix로 다른 시험구에 비하여 높은 함량을 보였다. 항산화물질인 총 폴리페놀과 플라보노이드 함량은 8번 시험구(8 hrs, 85℃, 40 mg/mL)에서 총 폴리페놀 함량이 30.40 mg GAE/g, 총 플라보노이드 함량이 146.50 mg QE/g으로 가장 높게 나타났으며, 16가지 시험구의 항산화 활성도 총 폴리페놀과 총 플라보노이드 함량의 결과와 같이 8번 시험구(8 hrs, 85℃, 40 mg/mL)에서 가장 높은 활성을 보였다. 이상의 결과를 참조하여 표고균사체 발효 적하수오에 대한 열수 추출조건을 최적화할 목적으로 추출온도를 95℃로 고정하고 용매비와 추출시간에 대한 종속변수들의 contour map을 superimposing하여 추출물의 특성 중 생리활성물질 함량과 생리활성을 만족시켜주는 최적조건은 91.22℃에서 39.71 mg/mL 용매비와 7.72 추출시간으로 각각 분석되었다. 본 연구는 적하수오에 표고균사체를 배양한 후 생리활성이 우수한 원료를 추출 생산하고자 최적 추출조건을 확립하기 위하여 반응표면분석법을 활용하였다. 향후 생리활성부분에 대한 추가적인 연구가 이루어진다면 더욱 완성도가 높은 표고균사체 발효 적하수오 식품개발에 가능할 것으로 사료된다.

Polygonum multiflorum (Polygonaceae family) is effective to cholesterol lowering effect, increased immunity effect and whitening effect. P. multiflorum root is used for medicinal herb that can be used as raw material for food. In this study, acetic acid fermentation was adding extracts of P. multiflorum root (0%, 1%, 3%, 5%) for the developing healthy beverage. In this study, we investigated the contents of free sugars, organic acids and inorganic components of P. multiflorum root. As a result of free sugars from P. multiflorum root analysis, three kind of free sugars were determined the glucose, sucrose and lactose by HPLC. Four kind of organic acids were found in lactic acid fermented P. multiflorum root. And oxalic acid, lactic acid and succinic acid were detected in organic acids. The content of lactose and lactic acid in lactic acid fermented P. multiflorum root was the highest among the free sugars and organic acids. The content of minerals were in high orders of sodium > calcium > magnesium in P. multiflorum by acetic acid fermentation. The content of inorganic components was highest in lactic acid fermented solution with 5% and proportional to the content of P. multiflorum

The purpose of this study was to expand the utility of the Polygonum multiflorum. Also, we fermented P. multiflorum by mushroom mycelial, and analyzed for chemical compositions and biological active of fermented P. multiflorum root. The crude protein content did not show a significant difference between control and fermented P. multiflorum root, crude fat, ash and crude fiber content of fermented P. multiflorum root were lower than control. The content of soluble nitrogen free extract of P. multiflorum root was significantly higher than fermented P. multiflorum root. The major amino acids of P. multiflorum root were determined the arginine. The content of arginine and glutamic acid were 586.67 mg%, and 283.78 mg%, respectively. Sixteen kinds of amino acids were detected in fermented P. multiflorum root, and the major amino acids were determined the arginine and threonine. The total amino acid contents of control and fermented P. multiflorum root were 3,469.03 mg%, and 3,630 mg%, respectively. As the results of antioxidant activity test, the antioxidant capacity of fermented P. multiflorum had a higher than the control. As the mushroom fermentation progresses, it is confirmed that the amino acid content and antioxidant capacity were increased, and it is expected to develop the product using the fermented P. multiflorum using mushroom mycelials.

Polygonum multiflorum (Polygonaceae family) is effective to cholesterol lowering effect, increased immunity effect and whitening effect. P. multiflorum root is used for medicinal herb that can be used as raw material for food. In this study, acetic acid fermentation was adding extracts of P. multiflorum root(0%, 1%, 3%, 5%) for the developing healthy beverage. In this study, we investigated the contents of free sugars, organic acids and inorganic components of P. multiflorum root. As a result of free sugars from P. multiflorum root analysis, three kind of free sugars were determined the glucose, sucrose and lactose by HPLC. Four kind of organic acids were found in lactic acid fermented P. multiflorum root. And oxalic acid, lactic acid and succinic acid were detected in organic acids. The content of lactose and lactic acid in lactic acid fermented P. multiflorum root was the highest among the free sugars and organic acids. The content of minerals were in high orders of sodium> calcium> magnesium in P. multiflorum by acetic acid fermentation. The content of inorganic components was highest in lactic acid fermented solution with 5% and proportional to the content of P. multiflorum

This study was performed to determine the optimal condition for Lentinula edodes JMI-10079 mycelium cultivation on the root of Polygonum multiflorum. We also analyzed the proximate composition, total amino acids, and minerals in the root of P. multiflorum cultivated with L. edodes JMI-10079 mycelia. The optimal temperature and pH for L. edodes JMI-10079 mycelium cultivation on the P. multiflorum root were 25oC and pH 5–6 respectively, whereas the optimal carbon and nitrogen sources were glucose and maltose, respectively. The content of crude protein, crude fat, and ash in the P. multiflorum root cultivated with L. edodes JMI-10079 mycelia was higher than that in the uncultivated P. multiflorum root. The content of crude fiber was the highest in the control. Total amino acid analysis revealed that the contents of total amino acids and total essential amino acids were increased by higher root of P. multiflorum concentration.

Many Wild Mushrooms were collected at Mt. Eokbul areas for 2 days in July 2010. and 1 day in August 2010. Identification in the collecting field by taking photos, recording the feature of the surroundings and ecological quality and then observing internal organs through an optical microscope. They were identified and examined with references. According to the result, Species diversities are 2 division, 2 classes, 1 subclass, 5 orders, 17 familys, 30 genera and 37 species. Dominant famillies are Tricholomataceae and Poryporaceae. Dominant species are Trichaptum abietum. Resources of Wild Mushrooms were 5 species in edibility, 6 species in clulture, 8 species in toxine, 6 species in medicne, 4 species in anticancer, 12 species in ectomycorrizahe and 16 species in rotten wood. Therefore the study is aimed at providing fundamental materials required for preserving Mt. Eokbul.

This study was performed to analysis of chemical constituent in Polygonum multiflorum root (PMR) by different dry methods (hot-air dry, shade dry, and freeze dry). The results are summarized as followings; major free sugar were detected fructose, glucose, and sucrose in dried PMR based on various dry methods. The highest content of free sugars was found in freeze dried PMR. The four organic acids were detected in dried PMR by HPLC analysis. The content of oxalic acid in shade dried PMR was higher than the dried PMR by different dry methods. The content of total amino acid and essential amino acids were high in the orders of freeze drying > shade drying > hot-air drying. The potassium and magnesium levels of freeze dried PMR was significantly higher than the other drying method of PMR. Whereas the calcium and sodium levels were higher in hot-air dried PMR. The major fatty acids were determined the linoleic acid in PMR by different dry methods.

The four transcription factors Oct4, Sox2, Klf4 and c-Myc have been used for making induced pluripotent stem cells. Many efforts have focused on reducing the number of transcription factors, especially c-Myc and Klf4 known as oncogene, for making induced pluripotent stem cells. Recently it have been demonstrated that Oct4 and Sox2 are able to reprogram human fibroblasts or cord blood cells to induced pluripotent stem cells and Oct4 has the ability to reprogram mouse and human neural stem cell to induced pluripotent stem cells. These researches imply cell types for reprogramming experiments have great influence on selection of reprogramming factors. Here we report that pig kidney cortex fibroblasts need only c-Myc factor when they are used for making induced pluripotent stem cells. We used two vector system including drug-inducible vector system and constitutive expression vector system. The two systems generate induced pluripotent stem cells from pig kidney fibroblasts successfully. These one-factor induced pluripotent stem cells are not only similar but also different to pig embryonic stem-like cells. These two one-factor induced pluripotent stem cell lines can express pluripotency related genes and be differentiated into all three germ layers in vitro. However, these two cell lines can be sub-cultured as a single cell by trypsin. Our results support that single factor, c-Myc, is sufficient to converting pig kidney cortex fibroblasts into induced pluripotent stem cells.