For the development of processed food for hypoglcemia, it is important to construct model system to confirm factors that reduce the glycemic index in real food. This study was to investigate the relationship between the gel model system and real food the high amylose type of segoami using response surface methodology. The independent variables were concentration (X1; 10, 15, 20, 25, and 30%) and steaming time (X2; 30, 35, 40, 45, and 50 min). The predicted glycemic index (Y1) was analyzed as a dependent variable. The regression of pGI was 0.7343, indicating that the model fits the data well in the prediction test. The predict glycemic index of the gel was in the range of 71.38 ~ 83.78, depending on the gel preparation conditions and predicted optimum condition was 23.7% gel concentration and 43.8 minutes of gelatinization. The predict glycemic index of rice gel were decreased with an increase of gel concentration, rather than the steaming time. In order to confirm the effect on in vitro digestibility in real food, the real food (Garraedduk) was prepared by applying the optimal conditions. As a result, there was no significant difference between the predicted value (77.1) and the experiment value (76.6). This result showed that the in vitro digestibility in real food can be predicted by applying the gel model system.

Entomopathogenic fungi have been used as important part of integrated pest management program to control aphid. In particular, Beauveria bassiana was distributed throughout the world including temperate and tropical area, various habitats from alpine soil, desert soil to running water and both insect and plant. Especially the fungus has also been isolated from the surface and the interior of plants and act as natural control agent. Viability of fungi on the plant surface may be influenced by temperature, humidity, sunlight and plant type as well as fungal isolate. Persistence of treated fungal control agent on phylloplane and control efficacy may differ from environmental conditions and isolates. In this study, we investigated the persistence of an B. bassiana which is developing as prototype wettable powder to control cotton aphid, and the residual efficacy of the prototype on cucumber under three different greenhouse conditions.

무등산국립공원은 백두대간에서 분기된 호남정맥의 핵심공간에 위치하고 있는 국립공원이다. 특히 백두대간과의 생태네트워크의 연결고리이자 호남정맥의 허브역할을 하는 공간이라 할 수 있으며, 일부 정상지역은 아고산대 식생대 분포특성을 나타내는 지역이기도 하고 주상절리대가 분포하는 지역으로 생태적으로나 경관적으로 매우 중요한 공간이라 할 수 있다. 특히 무등산은 광주광역시를 비롯해 화순군과 담양군의 진산 역할을 한 곳으로 역사와 문화적인 가치 또한 매우 높은 곳이라 할 수 있다. 이와 같이 생태적으로나 경관적인 가치, 지질학 가치와 역사‧문화 가치까지 뛰어난 지역이기 때문에 무등산은 2013년에 국립공원으로 지정되었다. 하지만 무등산국립공원은 국가안보의 현실적 상황으로 인해 국립공원에 지정되기 훨씬 이전인 1966년도부터 정상부에 대규모 군부대가 주둔하게 되었으며, 이로 인해 천왕봉과 지왕봉, 인왕봉에 이르는 정상부의 대규모 지형훼손까지 이루어지게 되었다. 이후 다양한 경로로 정상부 복원을 위해 노력해 왔으나 국방부의 반대로 인해 복원사업이 추진되지 못하고 있었는데 2015년 12월 3일에 국방부와 광주광역시, 국립공원관리공단이 ｢무등산 정상 군부대 이전 협약｣을 체결함으로써 군부대 이전이 가시화되었으며 이에 맞춰 본 연구를 수행하게 되었다. 본 연구는 지형 및 지질분야를 비롯하여 식생과 식물상, 탐방객, 훼손분야, 갈등 관리분야까지 다양한 분야별로 조사를 실시하였으며, 무등산국립공원 동부사무소의 적극적인 지원을 통해 과거에는 확인할 수 없었던 옛사진자료 등도 확보하였고 군부대 주변에 분포하는 관속식물에 대한 현황을 파악하였으며, 식생복원의 목표종과 목표군락을 설정할 수 있었다. 특히 정상부 중 천왕봉 지역에서 가장 심각한 지형훼손이 발생하였으며, 해발고도가 4m 정도 낮아진 것을 확인할 수 있었다. 이에 본 연구에서는 복원의 목표를 ‘무등산 정상부가 갖고 있는 역사적 가치를 비롯해 문화‧경관‧생태적 가치를 복원함으로써 무등산이 갖고 있는 고유의 가치를 복원한다.’로 설정하고 ‘무등산국립공원 정상부 복원위원회’의 구성을 제시하고 책임과 권한, 의무 등을 명시하였다. 특히 정상부 복원에는 상당한 재원이 필요하기 때문에 국민적인 공감대가 반드시 필요하며, 이를 위해서 복원 시나리오를 다양하게 설정하여 설명하고 복원의 수준을 결정해 나가야 할 필요성을 제시하였다. 즉 정상부 복원의 시나리오를 매우 다양하게 설정하고 대국민 여론수렴기간을 거치도록 하였다. 다만 본 연구진이 가장 합리적이라고 판단한 복원 시나리오는 인왕봉은 원형으로 복원하고 지왕봉은 현 수준을 유지하며 천왕봉은 최소한의 지형복원을 실시하는 복원 시나리오와 인왕봉은 원형으로 복원하고 지왕봉은 현 수준을 유지하며 천왕봉은 콘크리트 제거만 실시하고 정리하는 복원 시나리오를 추천하였다. 무등산국립공원은 시민이 만들어 낸 국립공원이지만 너무나도 많은 상처가 남아 있는 지역이라고 할 수 있다. 따라서 무등산국립공원 군부대 주둔지역에 대한 복원사업을 통해 지역과 공존하는 국립공원이라는 복원의 새로운 패러다임을 만들어 낼 수 필요가 있을 것이다.

Eight individual and blended chemical disinfectants were screened for preliminary evaluation of efficacy against duct organisms. Viable counts of surviving bacteria were determined after 30 min contact with each disinfectant. The single and blended disinfectants with high microcidal activity included glutaraldehyde/formaldehyde-based, glutaraldehyde/formaldehyde-based, cresol-based, organic acid-based and potassium peroxymonosulphate-based products. Iodophors of disinfectants showed an inconsistent and low anti-microbial effect.

Cotton aphid, Aphis gossypii Gloever is one of the major pests on a wide range of economically important crops in the world. The sustained use of chemical insecticides to control the aphid has led to the emergence of resistant strains to numerous used insecticides. As an alternative strategy entomopathogenic fungi have been used as part of integrated pest management program to control aphid, especially insecticide-resistance population. In particular, Beauveria bassiana-based commercial bio-insecticide has been used to reduce the pest population under greenhouse conditions in various countries. In this study, we investigated the control efficacy of a prototype of commercial mycopesticide using an B. bassiana (wettable powder) against cotton aphid on potted cucumber plant in greenhouse conditions.

The habitat of the cockroach varies by species. The German cockroach (Blattella germanica) lives in human dwellings, while the Japanese field roach (Blattella nipponica) lives in a mountainous region. Based on phylogenetic analysis of mtCO I, the two species are closely related to each other and B. germanica is divergent from wild species such as B. nipponica. Their habitats and walking speed differ even though the two species have similar morphology. We hypothesized that habitats might influence walking speed via changes to appendage morphology and enzyme-based physiological differences. We found that phenotype such as appendage length and esterase isozyme expression were clearly different between the two species. These differences might be responsible for the observed difference in walking speed.

Arsenic (As) is a toxic element that easily taken up by plants root. Several toxic forms of As disrupt plant metabolism by a series of cellular alterations. In this study, we applied annealing control primer (ACP)-based reverse transcriptase PCR (polymerase chain reaction) technique to identify differentially expressed genes (DEGs) in alfalfa roots in response to As stress. Two-week-old alfalfa seedlings were exposed to As treatment for 6 hours. DEGs were screened from As treated samples using the ACP-based technique. A total of six DEGs including heat shock protein, HSP 23, plastocyanin-like domain protein162, thioredoxin H-type 1 protein, protein MKS1, and NAD(P)H dehydrogenase B2 were identified in alfalfa roots under As stress. These genes have putative functions in abiotic stress homeostasis, antioxidant activity, and plant defense. These identified genes would be useful to increase As tolerance in alfalfa plants.

Clostridium perfringens (C. perfringens) induced necrotic enteritis (NE) and related subclinical disease has become economically significant problems for the broiler industry. The objectives of this study were to determine the toxinotype and the antimicrobial susceptibility of C. perfringens isolated from the intestine of NE-suspicious and clinically healthy chickens in Korea. All of the 110 isolates belong to C. perfringens type A. In addition, these strains harbored the luxS, colA, virS, cpb2, netB and tpeL genes in high percentage order. They do not harbor the cpe gene encoding enterotoxin production. Our results suggest the important roles of the several toxin genes in the pathogenesis of NE except α-toxin, previously thought to be the major virulence factor. In the antimicrobial susceptibility test of 13 antimicrobial agents, none of them is completely susceptible in this study. Remarkably NE-suspicious chickens have higher resistant value than health ones in all of antimicrobial agents. The presence of tetracycline-resistance genes were also tested to analyze the correlation of genotype and phenotype.

According to the regulations of the Korean Animal and Plant Quarantine Agency (QIA), offals include a greater diversity of internal organs than do those of other nations. The present study was conducted to investigate the microbiological quality of diverse edible offal products from pig and cattle slaughterhouses in Korea by performing aerobic plate counts (APCs) and E. coli counts. There were significant differences in E. coli values between red offal and white offal in both pigs and cattle. Significant differences in APCs were also observed between partial red offal and white offal of pigs (P < 0.05). However, no significant differences in APCs between red offal and white offal of cattle. The most frequently detected foodborne pathogen was Staphylococcus aureus (5.6% prevalence in pig offal and 12.5% % prevalence in cattle offal) followed by Clostridium perfringens (11.1 and 7.1%, respectively) and Salmonella (5.6% and 7.1%, respectively). None of the offal samples tested positive for E. coli O157:H7.

Various insect pests and plant disease can outbreak in a field. For the effective control of pests and plant diseases during crop cultivation, farmers simultaneously or sequentially spray various eco-friendly agricultural materials (EFAM), chemical pesticides and microbial control agents on the same fields. It was reported that many agrochemicals are harmful to entomopathogenic fungi, especially some fungicides with broad spectrum activity that are routinely applied for the control of plant diseases. In addition, some pesticides may antagonize the potential insecticidal activity and efficiency of entomopathogenic fungi. Therefore, sometimes the utilization of fungal entomopathogen in forestry and agricultural production is limited because of the undesirable interference from some fungicides and pesticides. There is little research that examines the compatibility of these EFAMs with entomopathogenic fungi and the influence of EFAMs on the control efficacy of mycopesticides. We conducted a study of influence of pretreated eco-friendly agricultural materials on control efficacy of Isaria javanica isolate against sweet potato whitefly.

Entomopathogenic fungi are natural enemies of insect pests and contribute to the natural regulation of their host populations. These fungal group are often used as active ingredients for microbial insect pest control. In addition, the potential antimicrobial effect by entomopathogenic fungi including Beauveria bassiana, Lecanicillium spp., and Isaria fumosorosea have recently been reported against fungal plant pathogens. Dual microbial control effects with entomopathogenic fungi against both aphids and cucumber powdery mildew had reported in Canada. In our previous studies we conducted bioassay with entomopathogenic fungi to develop dual microbial control agent which can control both aphid and fungal plant disease. We selected an Beauveria bassiana isolate which has high dual control effects against both cotton aphid, Aphis gossypii and sclerotinia rot, Sclerotinia sclerotiorum. In this study, we have tested the dual control efficacy of the B. bassiana isolate against cotton aphid and sclerotinia rot on whole potted cucumber plants. We found that the B. bassiana isolate protected the plant from cotton aphid and sclerotinia rot under laboratory condition.

경기도 8개 지역에서 2010년부터 2012년 동안 식균성인 노랑무당벌레의 발생기주를 조사한 결과, 흰가루병에 감염된 12종의 식물에서 관찰 이 되었다. 특히 가장 밀도가 높았던 배과원에서 노랑무당벌레는 7월 상순부터 11월 상순까지 발견되었다. 식균성인 노랑무당벌레의 장내에서는 흰가루병 균사나 포자 외에 다른 먹이의 흔적이 발견되지 않았고, 알과 번데기를 제외한 전 발육단계에서 균을 섭식하는 특성을 볼 때 절대적 식균성 곤충으로 생각된다. 25℃에서 오이 흰가루병균을 섭식한 노랑무당벌레의 발육기간은 알, 유충, 번데기, 성충이 각각 3.9, 10.4, 4.1, 37.7일 이었고, 발육단계별 오이 흰가루병 섭식량은 45.6, 144.4, 372.2, 628.1, 473.7 mm 2로 4령, 성충, 3령, 2령, 1령 순으로 많았다. 본 연구를 통해 노랑무당벌 레의 오이 흰가루병에 대한 섭식능력을 바탕으로 향후 유용 토착천적으로써 대량사육기술, 저독성 약제 선발 등 작물 흰가루병 종합방제기술(IPM) 에 대한 연구가 필요하리라 사료된다.