The effect of the surrounding vegetation on the seed germination and growth of mountain-cultivated ginseng (MCG, Panax ginseng C.A. Meyer) was investigated. Seed germination rate and growth were tested for allelopathy effects on four forest tree species after treatment with fallen leaves and leaf extracts. In the case of soil treatment through fallen leaves and crushed leaves, the germination rate was lower in the Quercus myrsinifolia treatments, and the average germination time was slower when Chamaecyparis obtusa was treated. In the case of Pinus densiflora and Quercus variabilis, which are used in most of the MCG cultivation areas, they did not have a significant effect on seed germination. In the fallen leaves treatments, the stem showed a tendency to lengthen. The hot water extract treatment showed a higher germination percentage than the cold water extract treatment. The extract treatment showed a deficient germination percentage of some MCG seeds. However, in the case of the treatments except for this, the germination percentage was similar to that of the control treatment. However, the Mean Germination Time, germination rate, and germination value were faster and higher than the control treatment. As a result of calculating the allelopathic index (AI) of MCG according to the extract treatment of 4 species, most had a negative effect on germination, and P. densiflora and Q. variabilis extracts showed the most significant effect. The ginsenoside content was higher in the fallen leaves treatment than in the control. The above results will help select and manage MCG plantations.

선박의 설계과정에 있어, 선박의 중량은 유체역학적 성능에 큰 영향을 미치는 가장 중요한 요소 중 하나이다. 선박은 일반적으 로 최적의 흘수와 배수량을 갖는 하나의 조건으로 설계되지만, 실제로는 연료의 소비, 선박 평형수의 충전과 적재 조건과 같은 운항 활동 으로 인해 선박의 중량 및 흘수가 일정 범위 내에서 바뀐다. 본 연구에서는 소형선박을 대상으로 3가지 하중조건에 따른 선박의 저항성능 변화를 모형실험과 수치해석을 통해 연구하였다. 마지막으로 2050년까지 CO2 배출 가스를 50% 감축한다는 국제해사기구(IMO) 목표를 따 라 선박의 저항 성능을 개선하여 동력 요구 사항을 줄이기 위해 선박의 중량 변화에 따른 저항성능의 민감도를 연구하였다. 연구 결과, 선박의 중량변화에 따른 효과는 낮은 프루드 수에서 크게 나타나는 것으로 확인되며, 저항성능에 대한 연구 결과, 설계 흘수의 적재조건 을 기준으로 배수량이 11.1% 증가하고, 흘수가 5% 증가한 Over load의 적재조건에서 운항 시 선체의 총 저항이 모형시험과 CFD 시뮬레이 션에서 각각 15.97%, 14.31%까지 증가하는 것을 볼 수 있다.

Spodoptera species (S. exigua and S. litura) are important pests of several crops and vegetables in Korea. We investigated development processes of Spodoptera species under constant temperatures (20, 25 and 30 oC) regimes and relative humidity (RH) (30-35, 50-55, 70-75, and 90-95%) conditions. We collected eggs of Spodoptera species by releasing them into a rectangular box inner walls covered with a sheet of white paper. Temperature and RH significantly impacted on oviposition, immature survival, adult emergence and longevity of Spodoptera species. Maximum number of eggs, shorter developmental time, higher adult emergence with longer longevity were reported in 70-75% at 30 oC. Minimal eggs and larval survival were recorded in 30-35% and 90-95% RH, respectively. This results suggest that temperature and RH had individual apparent effect on the developmental processes of Spodoptera species instead interactive effect. Therefore, there is chance to cause a significant damage to field crops and vegetables in 70-75% at 30 oC.

Although ethylformate and phosphine fumigants are widely used for pest quarantine, studies related to their mechanism of action and metabolic physiological changes in Drosophila models are still unclear. In this study, we investigated how key metabolites altered by fumigants and cold treatment are associated with and affect insect physiology by comparative metabolome analysis. Fumigant treatment significantly altered cytochrome P450 and glutathione metabolites involved in the detoxification mechanism and showed lower expression of PGF2α involved in the immune response compared to the control. Additionally, most of the metabolites functioned in metabolic pathways related to the biosynthesis of amino acids, nucleotides and cofactors.

Botanical extracts are employed in management of aphids. Extracts from Tanacetum cineariaiaefolium, Derris elliptica, and Sophora flavescens are widely used to control various insects. In this study, we determined concentrations of active insecticidal compounds (Ais) in commercial botanical extracts of these plants, and we investigated the time and concentration for lethal results with the green peach aphid, Myzus persicae. The concentrations of Ais, pyrethrins from T. cineariaiaefolium, rotenone from D. elliptica, and matrine and oxymatrine from S. flavescens, were determined after their fractionation by liquid chromatography followed by mass analysis and comparison with standard compounds. The extracts were tested for lethality in a bioassay with green peach aphids. Sprays at defined doses were applied to tobacco leaves infested with aphid nymphs. The lethal concentrations (LC50) were 20.4 ppm for pyrethrins, 34.1 ppm for rotenone, and 29.6 ppm for matrine at 48 h after treatments. At 100 ppm application levels, the lethal time LT50 was 13.4 h for pyrethrin, 15.1 h for rotenone, and 14.4 h for matrine. Kaplan - Meier analysis indicated the lethal times for the three botanical extracts at 100 ppm were significantly faster than application of a chemical insecticide, Sulfoxaflor, applied at the recommended level. These results provide baselines to develop and formulate single or mixed preparations containing botanical extracts to control green peach aphids on commercial crops.

주변 국가인 태국, 캄보디아, 베트남, 라오스 등에서 벼멸구(Nilaparvata lugens)와 흰등멸구(Sogatella furcifera)를 채집하던 중, 벼멸구와 형태가 아주 유사한 이삭멸구(N. muiri)와 벼멸구붙이(N. bakeri), 그리고 흰등 멸구와 형태가 아주 유사한 흰등멸구붙이(S. kolophon), 피멸구(S. vibix) 그리고 애멸구(Laodelphax striatellus)가 동시에 채집이 되는 등 형태적 차이점이 거의 없어 전문가도 쉽게 구분하지 못하는 문제가 있음이 확인되었다. 따라서 형태가 유사한 상기 멸구류의 종 동정을 확실히 할 수 있는 PCR용 프라이머의 개발을 위해 벼멸구 및 흰등멸구의 미토콘드리아 내 COI 영역을 특이적으로 검출할 수 있는 프라이머 세트를 제작하고 종 동정 효과를 확인하였다.

Many insects form mutualistic relationships with microbial symbionts, crucial for their physiological processes. The bean bug, Riptortus pedestris, establishes a unique gut symbiosis with the genus Caballeronia and consistently acquires these symbionts from surrounding soil with each generation. As a result, the bean bug unavoidably consumes a variety of environmental microbes, including potential pathogens. To address this, the bean bug has developed a specialized organ in its midgut that selectively filters out non-symbiotic microbes, thereby preventing contamination of its symbiotic organ. In this study, we identified a pathogenic strain from the genus Burkholderia that lethally affects the bean bug post-invasion of the symbiotic organ. This pathogen employs a strategy of mimicking the motility of native symbionts to infiltrate the symbiont sorting organ and displays a pronounced resistance against antimicrobial agents produced by the host. Upon establishing itself in the symbiotic organ, the pathogen breaches the midgut cells, leading to host mortality, and subsequently disperses into the external environment. Our findings unveil a cunning pathogenic tactic that exploits the mimicry of native symbionts within an insect's symbiotic framework.

최근 2년동안 한국에서 꿀벌의 소실현상으로 식량난이 벌어질 것이라는 우려가 있다. 2020년 기준 국내 12개 과채류에서 화분매개벌의 의존도는 67.2%로 농업에서 화분매개벌은 중요한 위치를 차지한다. 이번 꿀벌 소실로 인해 딸기, 수박, 참외에서 화분매개용 꿀벌의 수요공급 불균형 문제가 발생하였다. 딸기에서는 12월 중 꿀벌 조기소실 문제, 수박과 참외에서는 벌의 임대가격이 30-50% 상승과 더불어 벌을 구하지 못해 인력수급의 어려움 등 작물재배 농가에 큰 부담이 되었다. 꿀벌의 소실은 전세계적인 문제이며 복합적인 원인에 의해 발생하므로 단기간에 해결되기 어렵다. 따라서 꿀벌에 소실에 대비하여 안정적인 작물 생산을 위한 종합적인 화분매개 관리 가 반드시 필요하다. 첫째 화분매개용 꿀벌의 사양기술의 표준화를 통하여 기존 채밀위주의 사양기술보다 더 안정적으로 벌을 생산할 수 있는 기술이 개발되어야 한다. 둘째 작물에서 더욱 효과적으로 벌을 이용하고 관리할 수 있는 기술이 확립 보급되어야 한다. 셋째는 꿀벌을 대체할 수 있는 곤충자원(뒤영벌, 토종벌)을 이용한 화분매 개 기술을 개발해야 한다. 아울러 기후변화에 대응하여 장기적인 기상변동 데이터 확보와 더불어 야생 화분매개 자원의 보호 보존을 위한 연구가 수행되어야 할 것이다.