농가에서 곤충자원을 활용한 치유농업프로그램의 개발 요구가 증가함에 따라 기존 곤충 돌보기 중심의 치유프로그램과 더불어 활용할 수 있는 야간 곤충채집 활동을 치유농업프로그램으로 개발하 고 시범 적용하였다. 야간곤충채집 활동은 대학생 9명에게 사전에 참여 동의를 획득하고, 전남대학교 학술림(장성)에서 1박 2일간 진행하였다. 18시부터 24시까지 야간 채집 활동과 익일 오전에는 채집한 곤충을 이용한 곤충 표본 제작 활동으로 프로그램을 구성하였다. 프로그램의 효과 분석을 위해 사전 (활동 전), 중간(채집활동 직후), 사후(표본제작 직후)에 뇌파, 맥파 검사 그리고 설문조사를 실시하였 다. 뇌파 분석 결과, 야간 채집활동 직후 뇌파의 기초율동이 향상(10.67→11.44)하였다(p<0.05). 요인 분석을 통한 스트레스 분석 결과, 내적 스트레스는 3.22(사전)→2.96(사후)로 감소(p<0.1)하였고, 문제 수행능력 관련 스트레스는 2.92(중간)→2.70(사후)로 감소하였다. 이러한 결과는 야간 채집 활동 속에 서 이용되는 근육의 움직임이 뇌 기능 향상과 스트레스 감소에 영향을 줄 것으로 생각된다. 참여자가 적은 연구의 한계를 극복하기 위해 향후 연령대별, 성별 등 다양한 참여자를 대상으로 추가적인 채집 프로그램의 개발 및 적용이 필요하다.

Although ethylformate and phosphine fumigants are widely used for pest quarantine, studies related to their mechanism of action and metabolic physiological changes in Drosophila models are still unclear. In this study, we investigated how key metabolites altered by fumigants and cold treatment are associated with and affect insect physiology by comparative metabolome analysis. Fumigant treatment significantly altered cytochrome P450 and glutathione metabolites involved in the detoxification mechanism and showed lower expression of PGF2α involved in the immune response compared to the control. Additionally, most of the metabolites functioned in metabolic pathways related to the biosynthesis of amino acids, nucleotides and cofactors.

Botanical extracts are employed in management of aphids. Extracts from Tanacetum cineariaiaefolium, Derris elliptica, and Sophora flavescens are widely used to control various insects. In this study, we determined concentrations of active insecticidal compounds (Ais) in commercial botanical extracts of these plants, and we investigated the time and concentration for lethal results with the green peach aphid, Myzus persicae. The concentrations of Ais, pyrethrins from T. cineariaiaefolium, rotenone from D. elliptica, and matrine and oxymatrine from S. flavescens, were determined after their fractionation by liquid chromatography followed by mass analysis and comparison with standard compounds. The extracts were tested for lethality in a bioassay with green peach aphids. Sprays at defined doses were applied to tobacco leaves infested with aphid nymphs. The lethal concentrations (LC50) were 20.4 ppm for pyrethrins, 34.1 ppm for rotenone, and 29.6 ppm for matrine at 48 h after treatments. At 100 ppm application levels, the lethal time LT50 was 13.4 h for pyrethrin, 15.1 h for rotenone, and 14.4 h for matrine. Kaplan - Meier analysis indicated the lethal times for the three botanical extracts at 100 ppm were significantly faster than application of a chemical insecticide, Sulfoxaflor, applied at the recommended level. These results provide baselines to develop and formulate single or mixed preparations containing botanical extracts to control green peach aphids on commercial crops.

Leaf-spray in vitro bioassays appraise new aphicidal formulations for managing deleterious plant-feeding aphids. The formulation may utilize alternative and integrated strategies. However, leaf spraying even under controlled conditions may affect aphid reproduction and mortality. This study examines leaf spray applications for optimum and reproducible aphicidal results using tobacco leaves overlaid on cotton fabric or water agar surfaces. Infestation of the undersides of tobacco leaves with nymphs of green peach aphids was used in the assays. Spray distance and volume were optimized using water-sensitive paper to ascertain the best surface coverage. Overlays of the leaves on water agar caused less mortality and greater reproduction than the use of cotton fabric. The relative humidity of the insect-rearing chambers changed with the watering regime for the insect - rearing chambers with cotton fabric; 60% relative humidity was optimal. Relative humidity was not affected by the concentration of agar in the water agar chambers. Applications of the chemical aphicidal standard, Sulfoxaflor, under the optimized conditions exhibited similar times for lethality although the rate was faster with leaves on the cotton fabric than on water agar. These studies establish reproducible and sensitive techniques for assessing the lethality and effects on reproduction of potential aphicidal products.

주변 국가인 태국, 캄보디아, 베트남, 라오스 등에서 벼멸구(Nilaparvata lugens)와 흰등멸구(Sogatella furcifera)를 채집하던 중, 벼멸구와 형태가 아주 유사한 이삭멸구(N. muiri)와 벼멸구붙이(N. bakeri), 그리고 흰등 멸구와 형태가 아주 유사한 흰등멸구붙이(S. kolophon), 피멸구(S. vibix) 그리고 애멸구(Laodelphax striatellus)가 동시에 채집이 되는 등 형태적 차이점이 거의 없어 전문가도 쉽게 구분하지 못하는 문제가 있음이 확인되었다. 따라서 형태가 유사한 상기 멸구류의 종 동정을 확실히 할 수 있는 PCR용 프라이머의 개발을 위해 벼멸구 및 흰등멸구의 미토콘드리아 내 COI 영역을 특이적으로 검출할 수 있는 프라이머 세트를 제작하고 종 동정 효과를 확인하였다.

Rice serves as the staple food for many Asian countries. However, it faces a significant threat from various Hemiptera species, including Nilaparvata lugens, Sogatella furcifera, Laodelphax striatellus, and Nephotettix cincticeps, which can cause devastating diseases. These species are economically significant pests of rice. Traditional morphology-based methods have proven inefficient in accurately distinguishing these pests at the species level. In this study, we present a successful approach for designing species-specific primers and their application in both general and multiplex PCR as well as loop-mediated isothermal amplification (LAMP) assays, widely adopted molecular tools for species identification. Each primer set incorporates a species-specific sequence of at least 2 base pairs in both the forward and reverse primers. These primers have demonstrated exceptional diagnostic accuracy in conventional and multiplex PCR. Additionally, our study showcases the high sensitivity of LAMP, successfully achieving positive amplification with genomic DNA quantities ranging from 100pg to 10pg. In summary, these techniques provide an efficient means of diagnosing planthoppers in a large number of field-collected or individual samples.

모기는 감염병을 매개하는 종으로 전염병 확산 억제를 위해서는 개체수의 감시와 정확한 예측이 필요하다. 본 연구에서는 모기 개체수 및 기상 및 현장 자료를 활용해 모기 개체수 머신러닝 모델을 개발하였다. 모기 개체수는 디지털 모기 측정기(Digital Mosquito Monitoring System, DMS)의 2015 년~2022년의 5월~10월의 자료를 활용하였다. 기상 자료는 기온, 강수량, 풍속, 습도를 사용하였으며, 현장 조사 자료는 현장을 명목척도와 서열척도로 나누어 기록하여, 명목 척도의 경우 원핫 인코딩으 로 변환해 수치화하여 사용하였다. 분석에 사용된 머신러닝 모델은 Artificial Neural Network, Random Forest, Gradient Boosting Machine, Support Vector Machine이며 성능지표로 R2, RMSE를 사용하였다. 연구 결과, Gradient Boosting 모델이 R2 0.4, RMSE 22.45로 가장 좋은 성능을 나타냈다. 현장 조사 자료 를 분석에 활용하였을 때 R2는 증가하였고, RMSE는 감소하였다. 본 연구 결과 모기 개체수에 현장 조사 자료가 예측 정확도를 향상시킬 수 있음을 확인하였다.

Corn silage is extensively utilized in ruminant feeding on a global scale, with substantial research efforts directed towards enhancing its nutritional worth and managing moisture content. The purpose of this study was to assess the impact of normal cutting height and elevated cutting height on whole-crop corn silage. Corn was harvested at heights of 15 cm and 45 cm above the ground, respectively, 45 days after heading. The harvested corn was cut into 2-3 cm lengths and packed into 20-liter plastic silos in triplicate. The results showed that dry matter (DM), crude protein (CP), water soluble carbohydrates (WSC), and in vitro dry matter digestibility (IVDMD) of C45 were significantly higher than those of the control, while the neutral detergent fiber (NDF) was significantly lower in C45 (p<0.05). The C15 had higher yields than C45 (p<0.05). There was no significant difference in the total digestible nutrients (TDN) yield of whole-crop corn silage. The increase in cutting height resulted in a larger change in moisture content and NDF per centimeter. After 60 days-ensiling, C45 showed significantly lower NH3-N concentrations. Moreover, C45 had significantly higher lactic acid concentration, lactic acid/acetic acid ratio, and lactic acid bacteria count compared to the control. Mold was not detected and the yeast count was less than 2 log10 cfu/g fresh matter in both control and C45. In summary, C45 improved the feeding value and fermentation quality of whole-crop corn silage at the expense of forage productivity.

In the realm of dental prosthesis fabrication, obtaining accurate impressions has historically been a challenging and inefficient process, often hindered by hygiene concerns and patient discomfort. Addressing these limitations, Company D recently introduced a cutting-edge solution by harnessing the potential of intraoral scan images to create 3D dental models. However, the complexity of these scan images, encompassing not only teeth and gums but also the palate, tongue, and other structures, posed a new set of challenges. In response, we propose a sophisticated real-time image segmentation algorithm that selectively extracts pertinent data, specifically focusing on teeth and gums, from oral scan images obtained through Company D's oral scanner for 3D model generation. A key challenge we tackled was the detection of the intricate molar regions, common in dental imaging, which we effectively addressed through intelligent data augmentation for enhanced training. By placing significant emphasis on both accuracy and speed, critical factors for real-time intraoral scanning, our proposed algorithm demonstrated exceptional performance, boasting an impressive accuracy rate of 0.91 and an unrivaled FPS of 92.4. Compared to existing algorithms, our solution exhibited superior outcomes when integrated into Company D's oral scanner. This algorithm is scheduled for deployment and commercialization within Company D's intraoral scanner.

Due to the development of the internet and communications technology, the media market has undergone significant changes in the 2000s. In the past, when the types of media were not diverse, media content was mainly consumed through TV, radio, newspapers, etc., but as varied, it allowed users to watch the content anytime and anywhere. ‘Snack Video’ which is produced in a short length and consumed in commuting time and leisure time, and ‘Snack Culture’ which is a popular lifestyle trend of consuming short-form cultural content, is now widespread. The representative content of snack content is video clips. Various of media provide video clips, such as portal sites, SNS, and TV programs. YouTube is effective in increasing purchases than the other channels. Over-The-Top (OTT) is another pillar of the new media market, which has emerged with the development of the internet and communications technologies.

단단한 자구를 가진 적색 비모란선인장 ‘Gangjeok’ 품종 은 ‘Isaek’품종을 모본으로, ‘Suyeon’ 품종을 부본으로 하여 2018년에 교배하여 육성하였다. 교배 후 획득한 종자는 조직 배양실에서 기내파종하여 획득한 유묘를 기내에서 삼각주선 인장에 접목하여 ‘1802001’ 등 20계통을 양성하였다. 2019 년에 기내에서 양성한 20계통을 온실에서 삼각주선인장 대목 에 접목하여 재배하면서 ‘1812005’ 계통을 1차 선발하였다. 2020년부터 2022년까지 3차에 걸쳐서 특성을 검정한 후, 농 산물직무육성품종 심의회에서 최종 선발하여 ‘Gangjeok’으 로 명명하였다.‘Gangjeok’ 품종은 편원형의 적색 구를 가진 다. 혹(tubercle)이 돌출된 형태의 모구는 8.4개의 능(rip)을 가지며, 3.5mm 짧은 회색 가시가 발생한다. 정식 10개월 후 ‘Gangjeok’ 품종의 직경은 46.1mm이며, 자구는 평균 18.3 개 발생한다. 2022년 육성계통 평가회에서 ‘Gangjeok’ 품종 은 높은 기호도 점수 4.0을 받았다.

Biomass-derived porous carbon is an excellent scientific and technologically interesting material for supercapacitor applications. In this study, we developed biomass-derived nitrogen-doped porous carbon nanosheets (BDPCNS) from cedar cone biomass using a simple KOH activation and pyrolysis method. The BDPCNS was effectively modified at different temperatures of 600 °C, 700 °C, and 800 ℃ under similar conditions. The as-prepared BDPCNS-700 electrode exhibited a high BET surface area of 2883 m2 g− 1 and a total pore volume of 1.26 cm3 g− 1. Additionally, BDPCNS-700 had the highest electrical conductivity (11.03 cm− 1) and highest N-doped content among the different electrode materials. The BDPCNS-700 electrode attained a specific capacitance of 290 F g− 1 at a current density of 1 A g− 1 in a 3 M KOH electrolyte and an excellent longterm electrochemical cycling stability of 93.4% over 1000 cycles. Moreover, the BDPCNS-700 electrode had an excellent energy density (40.27 Wh kg− 1) vs power density (208.19 W kg− 1). These findings indicate that BDPCNS with large surface areas are promising electrode materials for supercapacitors and energy storage systems.

Natural uranium-contaminated soil in Korea Atomic Energy Research Institute (KAERI) was generated by decommissioning of the natural uranium conversion facility in 2010. Some of the contaminated soil was expected to be clearance level, however the disposal cost burden is increasing because it is not classified in advance. In this study, pre-classification method is presented according to the ratio of naturally occurring radioactive material (NORM) and contaminated uranium in the soil. To verify the validity of the method, the verification of the uranium radioactivity concentration estimation method through γ-ray analysis results corrected by self-absorption using MCNP6.2, and the validity of the pre-classification method according to the net peak area ratio were evaluated. Estimating concentration for 238U and 235U with γ-ray analysis using HPGe (GC3018) and MCNP6.2 was verified by 􀟙-spectrometry. The analysis results of different methods were within the deviation range. Clearance screening factors (CSFs) were derived through MCNP6.2, and net peak area ratio were calculated at 295.21 keV, 351.92 keV(214Pb), 609.31 keV, 1120.28 keV, 1764.49 keV(214Bi) of to the 92.59 keV. CSFs for contaminated soil and natural soil were compared with U/Pb ratio. CSFs and radioactivity concentrations were measured, and the deviation from the 60 minute measurement results was compared in natural soil. Pre-classification is possible using by CSFs measured for more than 5 minutes to the average concentration of 214Pb or 214Bi in contaminated soil. In this study, the pre-classification method of clearance determination in contaminated soil was evaluated, and it was relatively accurate in a shorter measurement time than the method using the concentrations. This method is expected to be used as a simple pre-classification method through additional research.

Kori-1 and Wolseong-1 nuclear power plants were permanently shut down in June 2017 and December 2019, and are currently in the preparation stage for decommissioning. In this regard, it is necessary to secure nuclear power plant decommissioning capacity in preparation for the domestic decommissioning marketplace. To address this, the Korea Research Institute of Decommissioning (KRID) was established to build a framework for the development of integrated nuclear decommissioning technology to support the nuclear decommissioning industry. The institute is currently under construction in the Busan-Ulsan border area, and a branch is planned to be established in the Gyeongju area. Recently, R&D projects have been launched to develop equipment for the demonstration and support verification of decommissioning technology. As part of the R&D project titled “Development and demonstration of the system for radioactivity measurement at the decommissioning site of a nuclear power plant”, we introduce the plan to develop a radioactivity measurement system at the decommissioning site and establish a demonstration system. The tasks include (1) measurement of soil radioactive contamination and classification system, (2) visualization system for massive dismantling of nuclear facilities, (3) automatic remote measurement equipment for surface contamination, and (4) bulk clearance verification equipment. The final goal is to develop a real-time measurement and classification system for contaminated soil at the decommissioning site, and to establish a demonstration system for nuclear power plant decommissioning. The KRID aims to contribute and support the technological independence and commercialization for domestic decommissioning sites remediation of nuclear power plant decommissioning site by establishing a field applicability evaluation system for the environmental remediation technology and equipment demonstration.

Wolsong unit 1, the first PHWR (Pressurized Heavy Water Reactor) in Korea, was permanent shut down in 2019. In Korea, according to the Nuclear Safety Act, the FDP (Final Decommissioning Plan) must be submitted within 5 years of permanent shutdown. According to NSSC Notice, the types, volumes, and radioactivity of solid radioactive wastes should be included in FDP chapter 9, Radioactive Waste Management, Therefore, in this study, activation assessment and waste classification of the End shield, which is a major activation component, were conducted. MCNP and ORIGEN-S computer codes were used for the activation assessment of the End shield. Radioactive waste levels were classified according to the cooling period of 0 to 20 years in consideration of the actual start of decommissioning. The End shield consists of Lattice tube, Shielding ball, Sleeve insert, Calandria tube shielding sleeve, and Embedment Ring. Among the components composed for each fuel channel, the neutron flux was calculated for the components whose level was not predicted by preliminary activation assessment, by dividing them into three channel regions: central channel, inter channel, and outer channel. In the case of the shielding ball, the neutron flux was calculated in the area up to 10 cm close to the core and other parts to check the decrease in neutron flux with the distance from the core. The neutron flux calculations showed that the highest neutron flux was calculated at the Sleeve insert, the component closest to the fuel channel. It was found that the neutron flux decreased by about 1/10 to 1/20 as the distance from the core increased by 20 cm. The outer channel was found to have about 30% of the neutron flux of the center channel. It was found that no change in radioactive waste level due to decay occurred during the 0 to 20 years cooling period. In this study, activation assessment and waste classification of End Shield in Wolsong unit 1 was conducted. The results of this study can be used as a basis for the preparation of the FDP for the Wolsong unit 1.