The study aimed to evaluate the usability of sterile bag collection (SBC) urinalysis and urine culture for diagnosing urinary tract infections (UTI). Urine culture is key for diagnosing UTI, and transurethral catheterization (TUC) or suprapubic aspiration is recommended for non-toilet-trained children. Although urine testing using SBC is non-invasive and easy, UTI can be diagnosed only if other criteria including clinical symptoms and positive urinalysis results are met. This study included 228 infants who were hospitalized for unexplained fever from October 2015 to June 2016. TUC culture, SBC urinalysis, and urine culture were performed for all patients. UTI was diagnosed when the TUC culture results met the criterion of ≥104 colony-forming units (CFU)/mL. When UTI diagnosis was made based on SBC urine colony counts ≥105 CFU/mL, the false-positive and false-negative rates were 6.3% and 70.0%, respectively. When the criterion was set as ≥104 CFU/mL, they were 23.7% and 30.0%, respectively. When both the criteria of ≥105 CFU/mL and positive urinalysis results were met, the false-positive rate was 2.4%, and the false-negative rate was 80%. Our results suggest that diagnosing UTI using SBC urinalysis and urine culture is not useful in infants with unexplained fever.

부산지역 의료종사자를 대상으로 병원감염관리 인식을 분석한 본 연구의 결론은, 먼저, 개인위생, 장비위 생, 청소 및 환경 관리의 인지도와 수행도는 학력이 낮을수록 척도가 높았다. 또한 개인손씻기의 경우 인지 도는 1차, 2차, 기타의료기관의 종사자들이, 수행도는 의료인 집단이 높은 수치를 나타냈으며, 유의수준 .00 1에서 통계적인 차이를 나타냈다. 병원 내 위생관리 역시 의료인이 높은 인지도와 수행도를 나타냈으며, 통계적인 차이를 보였다. 전체적으로 인지도에 비해 수행도가 낮아 감염관리에 대한 개인의 적극적인 수행 과 이를 위한 현실성 있는 개선방안의 마련이 필요할 것으로 사료된다.

본 연구는 대구 및 경북의 치과위생사들을 대상으로 치과 감염관리의 인식과 실천도를 조사하였으며, 이전의 연구를 참조하여 수정 및 보완 후 설문 조사하였다. 근무지 유형에 따라 치과의원보다는 치과병원에서, 치과 감염관리 담당자 및 감염관리 교육의 프로그램의 여부에 따라 다르게 나타남이 나타났다. 또한 치과 감염관리의 인지도와 실천도는 근무지 유형에 따라 개인방어, 무균술식법, 방사선 장비를 포함한 치과 장비, 감염성 폐기물과 세탁물 관리에서 유의하게 차이를 보였으며, 즉 치과 의원보다 치과병원이 감염관리 인지도 및 실천도가 높았다. 따라서 치과병원에서 감염 예방을 최소화하기 위해, 감염관리 및 감염관리의 정규 교육 및 가이드라인이 필요하며, 치과의 특성을 반영하는 지표 및 프로그램 개발 등이 빨리 도입 되어야 하며, 향후 치과의원(의원급 소형병원)에서의 감염 방지 및 평가 프로그램에 대한 연구 및 정부의 제도도 필요하다.

Toxocariasis is an illness of humans caused by roundworm. Toxocara species are commonly found in dogs and cats. Humans are accidental hosts. There are two routes of infection. One route is the ingestion of infected eggs, and the other route is the ingestion of an infected paratenic host. Clinical manifestations vary from asymptomatic infection to severe organ failure. In most cases, the patients are asymptomatic and the disease is self-limited. Thus, patients with mild symptoms do not require treatment. However we report on cases of patients with asymptomatic hepatic nodules who were treated to exclude malig-nant disease.

Reactive oxygen species (ROS) are produced in organisms as the natural products of oxidative metabolism by environmental stress and pathogen invasion. ROS, such as superoxide anion and hydrogen peroxide, can be toxic to cells and tissues to cause oxidative stress. Recent study revealed that olive flounder (Paralichthys olivaceus) superoxide dismutase (SOD) has been identified as a partial gene and strongly induced to benzoin[a]pyrene and it was deduced indicator of aquatic oxidative stress responses, but its transcriptional response against viral infection has not been investigated. In the present study, spatial and temporal expression profile was analyzed to investigate the function of Of-SOD in the anti-viral response. Of-SOD transcripts were ubiquitously detected in diverse tissues with variable levels using a real-time PCR. The expression of Of-SOD was significantly higher in the muscle, liver and brain, but extremely low in the stomach and spleen. Following VHSV challenge, the expression of Of-SOD increased within 3 hours and subsequently decreased to the original level at 2 days post-challenge in kidney. Although expression pattern and induction time are slight differences depending on the tissue, the transcript of Of-SOD was consistently increased in acute infection response, but expression is low in the chronic response. Collectively, Of-SOD expressions were inducible after VHSV infection and they were probably involved in the immune response against viral challenge. These results suggest that SODs may play important roles in the immune defense system of P. olivaceus and perhaps contribute to the protective effects against oxidative stress in this flounder.

In plants, eukaryotic translation elongation factor 1B (eEF1B) is composed of three subunits, eEF1Bα, eEF1Bβ and eEF1B γ. Two subunits are nucleotide exchange subunits (eEF1Bα and eEF1Bβ) and one is a structural protein (eEF1Bγ). In the previous study, eEF1B was identified as a common host factor for several RNA viruses. To test which subunit of eEF1B is essential for Potato virus X (PVX) replication, the virus-induced gene silencing (VIGS) for eEF1Bα, β or γ was performed in Nicotiana benthamiana and green fluorescent protein (GFP)-tagged PVX was inoculated. PVX-GFP accumulation was decreased when eEF1Bβ or γ subunit was silenced, whereas eEF1Bα had no effect on PVX-GFP accumulation in inoculated leaves. Targeting induced local lesions in genome (TILLING) was performed using a Capsicum annuum EMS population to test whether mutations in eEF1Bβ subunit affect virus infection in pepper. We obtained 81 eEF1Bβ mutant lines consisted of 16,759 individuals. These mutant lines are being tested to validate the function of eEF1B β in PVX replication.

식물이 병원체의 침입을 받으면 식물체내로 단백질을 전달한다. 병원체가 식물체내로 분비하는 단백질 중에서 cell death를 일으키는 단백질들에 대해 관심을 가졌다. 먼저 Pseudoperonospora cubensis 에 감염된 오이 잎에서 발 현되어 병원체 유래의 단백질들을 기존에 알려진 RLXR 도메인을 가진 염기서열에서 프라이머를 디자인 하여 PCR 반응을 통해 cloning 하였다. 이중에서 P. cubensis 접종시 발현이 증가하는 단백질들을 중심으로 Nicotiana benthamiana에 Infection 시켰다 그 결과 Infection된 Effector 들 중에서 2개의 Effctor에서 visible한 cell death를 관찰 할 수 있었다. 사세한 결과가 포스트를 통해서 발표될 것이다.

경상북도에 소재한 5개 종합병원 영상의학과의 오염도를 계수하고, 오염 원인균을 배양하여 동정하고 이를 토대로 지역사회 영상의학과의 효율적인 감염관리 방안을 모색하고자 하였다.확인된 표면오염 균주는 Staphylococcus, Micrococcus, Pseudomonas stutzeri, Pseudomonas oryzihabitans로 병원감염의 원인균으로 알려진 균주였고 영상의학과의 오염원으로 특이성을 가진 균주는 없었다. 조사대상 병원은 비슷한감염관리교육을 실시하고 있었으나 병원별 오염도의 차이는 유의한 차이를 보였다. 조사대상 병원별 접촉원에 따른 오염도의 차이는 방사선사 사용, 환자사용, 공통사용으로 구분하여 분석한 결과 환자사용이 방사선사사용, 공통사용에비해 유의하게 높은 오염도를 보였다(p<0.001).병원별 월평균 검사건수의 차이가 적은 장치는 모든 병원에서 유의한 차이를 보이지 않았으나 이에 비해 촬영건수가 가장 많은 일반촬영장치, 흉부전용촬영대의 오염도가 다른 촬영장치에 비해 오염도가 높았다. 또한 월평균 촬영건수가 많은 A, B, C 병원은 방사선사가 단독으로 사용하거나 또는 환자와 공통으로 사용하는 방사선조사장치, 사무용책상, 출입문 손잡이에서 비교적 높은 오염도를 보였다.

본 연구는 방사선사의 병원감염관리에 대한 인지도와 수행도를 파악하여 병원감염관리 방안의 개선 및 교육을 위한 기초자료를 제공하기 위해 실시한 연구이다.연구대상자의 병원감염관리에 대한 인지도와 수행도의 평균 평점은 150점 만점으로 인지도 141.05점과 수행도 138.15점으로 모든 영역에서 인지도가 높을수록 수행도가 높은 정상관계를 보였으나 인지도에 비해 수행도가 전체적으로 낮음을 확인 할 수 있었다.인지도에 영향을 미치는 요인으로는 감염관리 교육의 필요성, 감염예방 교육여부, 환자의 질병상태 인지 여부 등으로 통계적으로 유의하게 나타났고, 수행 도에 영향을 미치는 요인으로는 감염예방 교육여부, 환자의 질병상태 인지여부, 인지도 등으로 통계적으로 유의하게 나타났다.

The Cmr1 gene in peppers confers resistance to Cucumber mosaic virus isolate-P0 (CMV-P0). Cmr1 restricts the systemic spread of CMV-Fny, whereas this gene cannot block the spread of CMV-P1 to the upper leaves, resulting in systemic infection. To identify the virulence determinant of CMV-P1, six reassortant viruses and six chimeric viruses derived from CMV-Fny and CMV-P1 cDNA clones were used. Our results demonstrate that the helicase domain encoded by CMV-P1 RNA1 determines susceptibility to systemic infection. To identify the key amino acids determining systemic infection with CMV-P1, we then constructed amino acid substitution mutants. Of the mutants tested, amino acid residues at positions 865, 896, 957, and 980 in the 1a protein sequence of CMV-P1 affected the systemic infection. Virus localization studies with CMV-GFP clones and in situ localization of virus RNA revealed that these four amino acid residues together form the movement determinant for CMV-P1 movement from the epidermal cell layer to mesophyll cell layers. Quantitative real-time PCR revealed that CMV-P1 and a chimeric virus with four amino acid residues of CMV-P1 accumulated more genomic RNA in inoculated leaves than did CMV-Fny, indicating that those four amino acids are also involved in virus replication. These results demonstrate that the helicase domain is responsible for systemic infection by controlling virus replication and cell-to-cell movement. Whereas four amino acids are responsible for acquiring virulence in CMV-Fny, six amino acid (positions at 865, 896, 901, 957, 980 and 993) substitutions in CMV-P1 were required for complete loss of virulence in ‘Bukang’.

Pepper mild mosaic virus (PMMoV) and cucumber mosaic virus (CMV) are important pathogens in various vegetable crops worldwide. We have found that methanol extracts of Quercus dentate (Daimyo Oak) gallnut strongly inhibit PMMoV and CMV infection. Based on this result, the inhibitor named as "KN0912" formulated from the extract of Q. dentate gallnut was tested for its inhibitory effects on PMMoV or CMV infection to each local lesion host plant (Nicotiana glutinosa; PMMoV, Chenopodium amaranticolor; CMV). Pre-treatment effect of KN0912 against infections of each virus to local host plant was measured to be 75. 1:±0. 5-97. 5±1. 5% to PMMoV and 70.6±2.2-99.0±1.0% to CMV in 1-10mg/ml conc. and the absorption effect of the antiviral composition of KN0912 to the inside of tobacco leaves tissue, was inhibited by 55.7% to PMMoV and 63.8% to CMV. The persistence of KN0912 treatment was maintained until after the 3 days high inhibitory effect by 98% to PMMoV and by 95.1% to CMV. Inhibitory effects on systemic host plants of KN0912 were measured to be 80-90% to PMMoV and 60-75% to CMV. From the change of morphological characteristics of PMMoV particles under EM, we are tentatively suggested that one mode of action of KN0912 is inactivation due to the destruction of virus particles.

Questionnaires were distributed to Radiology departments at hospitals with 300 sickbeds throughout the Pohang region of North Gyeongsang Province concerning awareness and performance levels of infection control. The investigation included measurements of the pollution levels of imaging equipment and assistive apparatuses in order to prepare a plan for the activation of prevention and management of hospital infections. The survey was designed to question respondents in regards to personal data, infection management prevention education, and infection management guidelines. The ATP Public Heath Monitering System was used to measure seven items for pollution levels of imaging equipment and assistive apparatuses in the Radiology Department. Data was analysed using SPSS version 12.0 for paired t-test and Pearson coefficient with a statistically significant level of 0.05. The results of the survey showed a total awareness level of infection management prevention education averaged at 3.73 ± 0.64 and performance levels resulted at 3.39 ± 0.83 which were statistically significant (p = 0.01). Also the measurements of pollution levels for equipment with high patient contact showed a Pearson Coefficient of over 0.5 implying a focus on pathogenic bacterium. There was no statistical significance with the frequency of imaging (p < 0.05). Therefore for general hospitals with high patient contact, there is a need to supply analyzing equipment for real time monitoring and the implementation of disinfection management that uses a Ministry of Health and Welfare approved antiseptic solution twice every minute.

We previously demonstrated that root colonization of the rhizobacterium, Pseudomonas chlororaphis O6, induced expression of a galactinol synthase gene (CsGolS1), and resulting galactinol conferred induced systemic resistance (ISR) against fungal and bacterial pathogens in cucumber leaves. To examine the role of galactinol on ISR, drought or high salt stress, we obtained T-DNA insertion Arabidopsis mutants at the AtGolS1 gene, an ortholog of the CsGolS1 gene. The T-DNA insertion mutant compromised resistance induced by the O6 colonization against Erwinia carotovora. Pharmaceutical application of 0.5 - 5 mM galactinol on roots was sufficient to elicit ISR in wild-type Arabidopsis against infection with E. carotovora. The involvement of jasmonic acid (JA) signaling on the ISR was validated to detect increased expression of the indicator gene PDF1.2. The T-DNA insertion mutant also compromised tolerance by increasing galactinol content in the O6-colonized plant against drought or high salt stresses. Taken together, our results indicate that primed expression of the galactinol synthase gene AtGolS1in the O6-colonized plants can play a critical role in the ISR against infection with E. carotovora, and in the tolerance to drought or high salt stresses.