Caulis Bambusae in Taenia is widely used in Korea and China due to its various pharmacological activity. The present study aims to investigate the effect of the methanol extract of Caulis Bambusae in Taenia (CB) from Phyllostachys nigra Munro var. henonis Stapf (Gramineae) on amyloid β protein (25-35) (Aβ (25-35)), a synthetic 25-35 amyloid peptide, -induced neurotoxicity using cultured rat cortical neurons. CB, over a concentration range of 10-50μg/μl, inhibited the Aβ (25-35) (10 μM)-induced neuronal cell death, as assessed by a 3-[4,5-dimethyIthiazole-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay and the number of apoptotic nuclei, evidenced by Hoechst 33342 staining. CB (50 μg/μl) inhibited glutamate release into medium induced by 10 μM Aβ, (25-35) which was measured by HPLC. Pretreatment of CB (50 μg/μl) inhibited 10μM Aβ (25-35)-induced elevation of cytosolic calcium concentration ([Ca2+]c), which was measured by a fluorescent dye, fluo-4 AM, and generation of reactive oxygen species. These results suggest that CB prevents Aβ (25-35)-induced neuronal ell damage in vitro.

Resveratrol, which is both a phytoalexin with antifungal activity and a phytochemical associated with reduced cancer risk and reduced cardiovascular disease, is synthesized in a limited number of plant species including peanut. Resveratrol synthesis is catalyzed by the enzyme stilbene synthase including resveratrol synthase (RS). Resveratrol synthase gene (RS3) obtained from peanut, Arachis hypogaea, Fabaceae has been transferred into chinese foxglove, Rehmannia glutinosa by using Agrobacterium mediated transformation. RS t-DNA introduced to chinese foxglove (R. glutinosa L) by transformation and its reaction product, resveratrol-3-O-β-D-glucoside was isolated and characterized using HPLC. Also its biological effects was tested in inhibition of the lipid peroxidation of mouse LDL by glycosylated stilbenes derivatives obtained from transgenic plants. Resveratrol-3-O-β-D-glucoside isolated from transgenic R. glutinosa L. showed antimicrobial activity of the growth inhibition zone against Escherichia coli and Salmonella typhimurium. Therefore, this compound can be contributed to be useful as a phytoalexin for plant health as well as a phytochemical for human health.

In recent years, many researches are actively undertaken for environmental-friendly animal production according to the increased understanding about food safety because of the outbreak of various diseases such as mad cow disease, Foot and mouth disease and Poultry Influenza virus. However, high quality(higher safety)-animal production may not be successful without increasing of disease resistance of animal and the improvement of feeding environment. To increase the disease resistance is able to be accomplished by stimulating the immune function. The present study was undertaken to investigate the effects of enzyme mixture reinforced with β-glucanase activity which degrade polysaccharide to release β-glucan known as stimulator of immune function on the change of milk production and somatic cell count. After 12weeks of experimental feeding, milk production tended to be increased and somatic cell count was decreased from average 227×10⁴ to 37.1×10⁴. Milk protein and solid-fat content were tended to increase but milk fat showed decreasing tendency by the feeding of enzyme mixture. All together, it has been suggested that the improvement of high quality milk production may be possible through the dietary addition of immune modulating enzyme mixture in lactating dairy cows.

Total RNAs were isolated from cultured roots of Scopolia parviflora, poly(A)+ RNA was obtained through the mRNA purification, cDNA library of Hnh6h was constructed. Recombinant baculoviruses in Spodoptera frugiperda (Sf) cells were constructed by use of the transfer vector pBacPAK, which has the AcNPV sequence under the polyhedrin promoter. The expression vector carrying Hnh6h gene was transferred to S. parviflora and obtained transgenic hairy root lines. Our results confirmed the over expression of the H6H protein was used by anti-pBacPAK about cDNAs of S. parviflora. This study will served for production of tropane alkaloids by metabolic engineering.

귀리를 건강 및 기능성 식품소재로서 개발하기 위하여 귀리 겉겨(OBC)로부터 수용성 -glucan을 중심합성실험계획에 의해 분리하고, 생물활성(항균, 항산화, 암세포 생육저해능)을 검토하였다 귀리 수용성 -glucan 시료는 250, 500/disc농도에서 항균활성을 paper disc method로 검토한 결과 항균력이 없었으며, 5%농도에서 전자공여능(electron donating ability, EDA)으로 측정한 항산화활성도 없었다. 귀

온대 수도작에 있어서 발아시의 조건에 가까운 저온.담수 토양조건에서의 출아에 관련된다고 생각되어지는 β-아밀라제 유전자 발현과 발현 양상이 실제로 호분층 인접 부분의 전분 분해에 영향을 미치는지 in situ hybridization과 현미화학적방법으로 검토하였다. 1. 18℃ 의 저온.담수토양조건하에서 출아했던 장향도 품종은 호분층에서 β-아밀라제 유전자의 발현이 보였다. 2. 18℃ 의 저온.담수토양조건하에서 출아하지 못했던 수원 287호는 β-아밀라제 유전자의 발현이 보이지 않았다. 3. β-아밀라제 활성의 유무에 의해 배반세포에 인접한 배유부분의 전분분해량에 변화를 보여 β-아밀라제 활성이 높은 장향도, 은방주(Ginbozu), Fortana I-133가 18℃ 의 저온.담수토양조건하에서는 β-아밀라제 활성을 나타내지 않는 수원 287호와 시험한 모든 조건하에서 β-아밀라제의 활성이 보이지 않았던 농림(Norin) 6호, 고시히까리(Koshihikari) 보다 배반상피세포 및 배반상피세포에 인접한 호분층 근접 배유부분의 전분립 감소가 컸다. 이상의 결과 저온.담수토양조건하에서 벼 종자의 출아에 β-아밀라제 유전자의 발현과 전분 분해의 연관 가능성을 확인하였다.

Antibodies raised against the purified p-subunit of β -conglycinin were used in immunohistochemical studies to monitor the pattern of β -conglycinin mobilization in the cotyledons during soybean [Glycine max (L.) Merr.] seed germination. Western blot analysis revealed that the break down of the β -subunit of β -conglycinin commenced as early as 2 days after seed imbibition (DAI). Concurrent with the degradation of the β -subunit of β -conglycinin, accumulation of 48, 28, and 26 kD proteolytic intermediates was observed from 2 to 6 DAI. Western blot analysis also revealed that the acidic subunit of glycinin was mobilized earlier than the basic subunit. The basic glycinin subunit was subjected to proteolysis within 2 DAI resulting in the appearance of an intermediate product approximately 2 kD smaller than the native basic glycinin subunit. In contrast to the major seed storage proteins, lipoxygenase was subjected to limited proteolysis and was detected even after 8 DAI. The first sign of β -conglycinin breakdown was observed near the vascular strands and proceeded from the vascular strands towards the epidermis. Protein A-gold localization studies using thin sections of soybean cotyledons and antibodies raised against the β -subunit of β -conglycinin revealed intense labeling over protein bodies. A pronounced decrease in the protein A-gold labeling intensity over protein bodies was observed at later stages of seed germination. The protein bodies, which were converted into a large central vacuole by 8 DAI, contained very little 7S protein as evidenced by sparse protein A-gold labeling in the vacuoles.

This study was conducted to investigate the β-glucan contents and their characteristics of winter cereals according to particle sizes and milling recoveries. Sieved fractions differed in their average contents of β-glucans, and the coarse fraction had higher contents of β-glucan than finely milled fractions. In all winter cereals, the β-glucan contents of raw flours were higher than those of their brans, and the highest β-glucan contents of every cereals were observed at 100 mesh 〉 or 100-140 mesh fractions except the Chalssalbori fractions which showed the higest β-glucan contents (12.9%) at 140-200 mesh fraction. As compared with the β-glucan content of Chalbori among the various milling recoveries, the β-glucan was distributed more evenly throughout the endosperm but β-glucan content in bran of Chalbori was only 1.5%. However, β-glucan content of Chalssalbori (hull-less waxy barley) was the highest in the subaleurone region (8.2%) and declined slightly toward inner layers of grain. This results suggest that β-glucan distribution between high (Chalbori) and low β-glucan barley (Chalssalbori) may explain the difference in milling performance of barley. On the other hand, β-glucan contents of two rye varieties (Chilbohomil, Chunchoohomil) were lower than those of two waxy barley varieties, and the higest β-glucan contents were observed at the 60% milling recoveries. In all winter cereals, the L-values (lightness) of raw flours were higher than those of brans. And the L-values of barley varieties were higher than those of oat and rye varieties. As the particle sizes and milling recovery ratios were decreased, the L-value were increased. The a-values (redness) in brans of every winter cereals were higher than those of every particle size flours and every milling ratio fractions, and this tendency was observed in the b-values (yellowness) of every particle size of cereal flours. The L and b-value of barley, the b-value of oat, and L, a, b-value of rye have the significant relationship with the β-glucan contents, respectively. This results represent the fact that β-glucans affected the color of the flours and pounded grains of winter cereals.

In ultrastructure study of testis, Sertoli cells start to differentiate at 16 days of gestation. Transcripts of FSH receptor, IGF-I receptor, ER receptor and androgen receptor were highly and initially expressed at 16 day of gestation. As results of in situ PCR at 16 day of gestation, transcripts of FSH, IGF-I receptor were detected in Sertoli cells and spermatogonia, whereas the receptors of and androgen were detected in Sertoli cells. Therefore, expression of FSH and estrogen androgen, IGF-I and could play an important role during fetal and prepubertal testicular development by stage specific manner in mouse.

-Galactosidase was extracted and purified from strawberry. The purified -Galactosidase from strawberry was investigated their physicochemical characteristics. -Galactosidase was purified 25.74 fold from strawberry. The purification procedure include ammonium sulfate fraction, acetone powder treatment and gel and ion exchange chromatography. Yield of the enzyme purification was 18.11%. The purified enzyme has native molecular weight of 116,000 dalton. Vmax value and Km value of -Galactosidase were 0.077 mM ONPG/ml/15mim and 1.75x10-2mM, respectively. The optimum temperature and pH of -Galactosidase were 43C and pH 4.0, respectively. The -Galactosidase activity was stable below 50C and at pH 4.0 to pH 6.0. Among the metal ions Ca and Mg were did not affect, whereas K, Cu and Zn show a little effect on the enzyme activity. The -Galactosidase activities were inhibited by treatment with EDTA and SDS.

The experiment was carried out to study the variations and geographical distribution of β -amylase isozyme by isoelectric focusing (IEF) within Korean, Chinese and Japanese soybean land races. In pH 3-10 gel of IEF, the amylase of soybean accessions was separated into low pI group isozymes (TEX>Sp1 b) and high pI group isozymes(Sp1 a). In pH4-6.5 gel, isoelectric points were at 5.07, 5.15, 5.25, 5.40, and 5.94, and h, j, and k bands also were found. The distribution of Sp1 a allele (high pI type) was 29.3% in soybean accessions from Korea, 10.1 % in those from China, and 6.9% in Japanese accessions. The percentage of Sp1 a) allele was the highest in soybean accessions from Kyungsang province (35 %) in Korea, then central China (32 %) in China, and Honshu (10%) in Japan