In this study, to stabilize the heavy metal in the contaminated soils, the column leaching test based on rainfall and pH value was performed by using coal mine drainage sludge(CMDS): which was generated during electrical purification of abandoned coal mine wastewater. Four types of testing column were used in this study. That were the CMDS and the heavy metal contaminated soils well mixed in 0 wt%, 1 wt%, 3 wt% and 3 wt% layered column. According to the investigation, when the influent pH was 5.5∼6.2, there were no heavy metal elution at all conditions, and when the influent pH was 3∼3.3, the order of Cu, Zn, Pb, Cr elution concentration was 3 wt% M(mixed)＜3 wt% S(separation)＜1 wt% M＜0 wt% and the average elution concentration was quite low, the value was 0.005 mg/L. Therefore, CMDS can used as new stabilizer of the heavy metal in the contaminated soils.

본 연구는 환경친화적인 중금속 정화방법인 식물복원법(phytoremediation)의 적합성 규명을 위해 경관적 가치를 제공할 수 있는 조경수인 개나리(Forsythia koreana Nakai), 명자나무(Chaenomeles speciosa Koidz) 와 쥐똥나무(Ligustrum obtusifolium )Sieb. et Zucc.의 Cd처리에 따른 생육량과 생리적 특성을 조사하였고, 식물체내의 중금속 축적량을 확인하여 중금속 오염지에 대한 식물복원 개량대책을 위한 가능성을 알아보고자 실시하였다. 생장반응에 있어 Cd의 영향은 생장량의 감소현상은 뚜렷하였으며, 쥐똥나무 경우 6월 7일경에 무처리구는 신초장 60.10cm를 보였으나, Cd 처리구는 11.50cm로 생육이 감소하였다. 한편 명자나무에서는 Cd 처리구에서 가장 생육이 극히 부진하여 Cd에 내성이 약한 것으로 나타났다. 또한 Cd 처리에 따른 생리적 특성에 있어 엽록소 함량은 개나리에 있어 Cd 처리구에서 3.36 mg/g으로 4.85 mg/g인 대조구에 비해 현저하게 감소하였고, 쥐똥나무에서도 4.17mg/g에서 2.57mg/g으로 현저한 감소하는 경향을 보였다. 또한 TSC 함량의 변화는 엽록소의 변화에 매우 유사한 경향을 보였다. Cd 처리에 따른 전 질소 함량의 변화는 쥐똥나무의 Cd 처리구에서 가장 낮은 6.82를 보였다. 한편 soluble protein 함량에 있어서는 모두 Cd 처리구에서는 대조구와 큰 차이가 없었다. 한편 Cd에 대한 조사대상 식물들의 축적정도는 개나리에 있어 10.36ppm인 지하부의 Cd 중금속의 축적량은 2.37ppm인 지상부에 비해 4.3배 정도 많았고, 명자나무는 지상부 1.06ppm에 비해 지하부가 7.17ppm로 6.7배이며, 쥐똥나무는 지상부 함량이 3.23ppm인 반면에, 지하부가 5.12 ppm으로 1.5배로 높게 나타냄을 알 수 있다. 식물체의 지하부 축적량을 비교하면 개나리>명자나무> 쥐똥나무 순으로 볼 수 있으나, 지상부에서는 쥐똥나무>개나리>명자나무 순으로 중금속의 축적량이 가장 많았다.

This study was performed to examine the accumulated concentrations (conc.) of cadmium (Cd) in the organs of Arabidopsis thaliana grown in the soil with different conc. of Cd. The official standard conc. of Cd of pollutant exhaust notified by the Korean ministry of environment (0.1 mg/L) and ten times higher (1 mg/L) and fifty times higher (5 mg/L) conc. and no Cd in the soil as control were used for this investigation. The results showed that accumulated conc. of Cd in the stems of plant grown in the soil with different conc. (0.1, 1 and 5 mg/L) were increased 9%, 24% and 286% respectively, compared with normal plant stem. The accumulated conc. of Cd in the leafs of plant grown in the soil with official standard conc. and conc. ten times higher and conc. fifty times higher were increased 3%, 22% and 453%, respectively, compared with normal plant leaf. The accumulated conc. of Cd in the root of plant grown in the soil with 0.1 and 1 mg/L conc. of Cd were increased 6%, 19%, respectively, compared with normal plant root. However, it was observed about 84% of increased accumulation of the Cd in the root of plant, when highest (5 mg/L) conc. was used. The accumulated conc. of Cd in the different organs of Arabidopsis thaliana were increased according to increase of Cd conc. in the soil. When official standard conc. and ten times higher conc. of Cd were used, the accumulated conc. of Cd increased average 6%, 21%, respectively, compared with normal plant organ, and the accumulated conc. of Cd between leaf, stem and root were not significant. However, the accumulated conc. of Cd in the plant organs grown in the conc. fifty times higher were increased about 285%, compared with normal plant. In addition, the accumulated conc. of Cd in different organs of Arabidopsis thaliana exhibited wide differences between organs, that is, stem was increased 118% than root, leaf was increased 256%, 64% than root and stem, respectively. These results show that accumulated conc. of Cd in Arabidopsis thaliana with highest (5 mg/L) conc. of Cd in soil, were much higher in the leaf than the stem or root in proportion to the conc. of Cd contaminated within the soil.