Purpose: This study aimed to explore user experience with UV-C disinfection devices in clinical settings to obtain critical information for the development of domestic devices to meet the increasing demand for efficient environmental disinfection in healthcare settings, particularly in terms of effective multidrug-resistant organism control. Methods: A qualitative approach was employed involving 21 participants (infection control nurses, staff nurses, and device managers). Data were collected through five sessions of focus group interviews, and conventional content analysis was undertaken. Results: Four categories and 13 subcategories were identified: (i) Introduction and usage status of UV-C disinfection devices, with ‘Surge in demand for rapid response to infectious diseases’ and ‘Diversified application and management of UV-C disinfection devices based on needs’ subcategories; (ii) Advantages of using UV-C disinfection devices, with ‘Significantly reduced disinfection time,’ ‘Easy and simple operation,’ and ‘Providing a sense of reassurance from a visible confirmation of disinfection’ subcategories; (iii) Limitations of current UV-C disinfection devices, including ‘Ambiguous disinfection range and presence of disinfection blind spots,’ ‘Lack of standards for disinfection efficacy verification and management,’ ‘Safety concerns regarding ultraviolet radiation exposure,’ and ‘Issues related to maintenance of UV-C disinfection devices’ subcategories; and (iv) Expectations for domestic UV-C disinfection devices, including ‘Minimization of disinfection blind spots,’ ‘Variety in device sizes,’ ‘Auxiliary devices for enhancing usage efficiency,’ and ‘Clear protocols for device usage and maintenance. Subcategories.’ Conclusion: UV-C disinfection devices offer valuable benefits for infection control; however, improvements are needed to enhance their efficacy and usability. Practical recommendations include developing standardized safety protocols, enhancing UV-C coverage, and improving device mobility and maintenance to meet diverse healthcare needs. Such advancements in UV-C technology can significantly support effective infection control and operational efficiency in healthcare settings.

High-risk microbial pathogens are handled in a biosafety laboratory. After experiments, the pathogens may remain as contaminants. To safely manage a biosafety laboratory, disinfection of microbial contaminants is necessary. This study was carried out to evaluate the effect of UV-C irradiation for the disinfection of a high-risk plant pathogenic bacterium Erwinia amylovora in a laboratory setting. For the test, the bacterium (8.7 × 106 CFU/ml) was embedded on the surface of PDA and placed on the work surface in a biosafety cabinet (Class 2 Type A1), and on the three different surfaces of the laboratory bench, laboratory bench shelf, and the floor which were positioned in a straight line from the UV lamp installed in the ceiling of the biosafety laboratory (BSL 2 class). UV-C irradiation was administered for 10min, 30min, 1 hr, 2hr, 3 hr, and 4hr, respectively. The reduction rate of bacteria ranged from 95% to 99% in regard to 10 min irradiation, from 97% to 99% in regard to 30 min irradiation, from 99.8% to 99.9% in regard to 1 hr irradiation, and higher than 99.99% in regard to 2 hr irradiation. The bacterium was completely inactivated after 3 hr irradiation. A similar UV-C irradiation effect was obtained when the bacterium was placed at a distance of 1 m from the three different surface points. Bacterial reduction by UV-C irradiation was not significantly different among the three different surface points.