Sunlight, and in particular its UV component, is the major environmental trigger that underlies the major signs of human skin and skin cancer in general. Therefore, this study was carried out to investigate the UV protection effects of R. coreanus. R. coreanus was extracted by ultra high pressure extraction process at 500 MPa and 30℃ for 5 and 15 minutes. The cytotoxicity of the extracts extracted by ultra high pressure process on human dermal fibroblast cell CCD-986sk, human kidney normal cell HEK293, and human lung normal cell HEL299 was measured as 17.5%, 16.5% and 14.0%, respectively in adding 1.0 mg/ml of the samples, which was much lower than that from conventional water extraction method at 100℃ as 23.2%, 22.5%, 21.2%. The secretion of NO- from macrophage showed 15.9 μM on the R. coreanus extract from this process, which was higher than others. Prostaglandin E2 (PGE2) production from UV-induced human skin cells was also greatly decreased down to 510 pg/ml, compared to the control. From the results, we considered that the extracts from R. coreanus could be potent natural materials for skin anti-inflammation agent, and could be used as a potential anti-aging for the photo-damaged skin.

This study was conducted to develop an efficient cryopreservation method of human embryonic stem (ES) cells using vitrification. In an initial experiment, sub-clumps of human ES cells (CHA-hES3 and CHA-hES4) were vitrified using grids after incubation with STO feeder cells for 1 or 16 h (Groups 1-1 and 1-2, respectively). After storage for months, thawed clumps were re-plated on a fresh feeder layer. The survival rates of warmed CHA-hES3 and CHA-hES4 cells of Group 1-2 were significantly higher than those of the corresponding Group 1-1 cells. In the second experiment, human ES cells were vitrified after incubation with feeder or feeder-conditioned medium (Groups 2-1 to -7). Relative mRNA expression of BM proteins and survival rates were increased following incubation of ES cells with fresh feeder cells for 16 h. In conclusion, increasing of tight adhesion between ES cells by extended incubation with feeder could reduce cryoinjury after vitrifying/warming.

The purpose of this qualitative study is to examine the literatures about human health effects due to the climate change and ozone depletion since 1990s. Over the past decade climate becomes an increasingly significant factor in world affairs because of its effect on food supplies, energy consumption, environmental quality and natural hazards etc. Simultaneously the scientific associations have had to reappraise the nature and scope of climatology, increasing political relevance, leading to a growth in interdisciplinary research involving natural and socioeconomic scientists. The 2000s promise to continue this broadening of scope as the three-way interaction of human society, medical system and atmospheric system are further explored and evaluated. This paper reviews this trends, paying particular reference to climate-based environmental concerns and human health effects being debated.

Reactive oxygen species(ROS)에 의한 산화적 손상은 냉동보존 과정과 체외 배양과정 중 세포 생존률 감소의 주된 요인 중 하나이며, 특히 줄기세포의 경우 냉동보존 후 쉽게 분화하거나 사멸하는 경향이 있음이 잘 알려져 있다. 따라서 본 연구는 체외 배양된 인간 조혈모 줄기세포의 냉동보존 시 선별된 항산화제를 처리하여 항산화제가 줄기세포의 생존 및 자동분화에 미치는 영향을 조사하고자 하였다. 해동 후 세포의 생존률은 -tocopherol

본 연구는 해운서비스 거래기업간의 관계를 대상으로 관계마케팅 기법을 적용함으로써 보다 동태적인 시장메카니즘을 분석하고자 수행되었다. 해운기업은 영업업무가 큰 비중을 차지하는 기업으로서 해운기업과 하주의 관계구조를 분석함에 있어서 고객의 접점에 있는 영업사원의 관계특성을 기업차원의 관계특성과 함께 고려해야할 필요가 있다. 따라서 본 연구는 해운서비스 공급기업에 초점을 맞추어 하주들과 장기협력관계를 구축하는 데 영향을 미치는 관계의 질을 중심으로 그 선행요인인 관계특성과 성과요인인 장기협력관계를 실증분석함으로써 해운기업이 하주와 장기협력관계를 구축하는 데 유용한 정보를 제공하고자 하였다.

본 연구는 압화를 이용하여 다양한 작품을 제작하는 원예치료 프로그램을 통해 여성 정신분열증 환자의 무력감 감소와 인간관계에 미치는 영향을 알아보고자, 전남 영광에 위치한 기독 신하정신병원 개방병동의 여성 정신분열증 환자 7명을 대상자로 선정하여 원예치료 프로그램을 6월 20일부터 12월 18일 까지 주 1회 실시하였다. 대상자 7명의 치료효과를 측정하기 위해 원예치료 프로그램 전과 후에 무력감과 인간관계 측정지를 이용하여 평가하였고, 프로그램 초반부와 후반부에 대상자들의 특성을 조사하였다. 대상자 7명 모두가 원예치료 프로그램을 실시한 후 무력감이 감소한 것으로 나타났고, 대상자 전체의 무력감 평균치는 프로그램 실시 전 28.14에서, 실시 후에는 22.43으로 낮아졌다. 인간관계는 대상자 7명 중에 5명이 원예치료 실시 전과 비교해 보았을 때 호전되었고, 전체 대상자의 인간관계 측정치의 평균도 프로그램 실시 전에는 63.50이었는데, 실시 후에는 69.67로 높아졌고 통계적으로도 유의한 것으로 나타났다.

The present study was conducted to determine the effect of recombinant human follicle stimulating hormone (rhFSH) on the estrogen synthesis by human fetal ovarian tissues. Fetal ovaries were 18-19 weeks old (18 wks:n=1, 19 wks:n=2). One case of 19-week-old fetal ovaries were obtained from anencephalic fetus. Obtained ovarieswere cleaned and diced around pieces, and cultured in the sandwich agar bed system for 21-23 days. Estrone () and estradiol-17 () in the medium was measured by radioimmunoassay. Amount of synthesis was greater than in both normal cases. In contrast, fetal ovaries from anencephalic fetus did not produce neither nor in the presence or absence of rhFSH. Results suggest that the fetal ovaries have a capacity of estrogen production at 18-19 weeks of gestation Existence of FSH receptor is also supposed. Different results by anecephalic fetal ovaries suggest the difference in the development between normal and anencephalic fetal ovaries.

The safety of the ship manoeuver is dependent not only on the inherent characteristics of the ship itself and environmental conditions, but also on the skill and experience of the ship operator. As the ship operator's judgement is related to his experience and skill, based on outside information, piloted controllability also depends upon the effectiveness, accuracy and reliability of various information which is obtainable from navigational equipments and aids to navigation that are available to the ship ope-rator. Although these factors, human factors and informational factors, have been pointed out as major factors which affect the controllability of a ship, there was no a comprehensive examination on the effect of these two factors. In this thesis, therefore, an attempt was made to examine whether or not there is clear indication that these two factors affect the controllability of a ship in reality. Experiments were conducted using the port design simulator of the Korea Maritime University.