Bis(2-ethylhexyl) phthalate (DEHP) is one of the plasticizers used in the polyvinyl chloride (PVC) industry. It is known to be easily released into the environment. In this study, we investigated effects of DEHP on growth, metabolic pathway, and virulence gene expression in soil-borne bacterial plant pathogen, Pectobacterium carotovorum SCC1 using in vitro assays. As a result, DEHP at 20 μg mL-1 did not affect the growth, cell membrane permeability, or ATPase activity of P. carotovorum SCC1. However, it decreased succinyl-CoA synthase (SCS) activity in the tricarboxylic acid (TCA) cycle. Relative expression levels of virulence genes encoding pectate lyase and pectin were differentially influenced by DEHP treatment. These results suggest that biological characteristics of P. carotovorum might be influenced by DEHP in soil.

sodium bis-(2-ethylhexyl)sulfosuccinate-water 라멜라 액정의 비뉴톤 유동곡선을 cone-plate 레오메타를 사용하여 여러 농도와 온도 조건에서 얻었다. 이러한 비뉴톤 유동곡선을 비뉴 톤 유동식에 적용하여 유동파라메타를 구하였다. 특별히 주목할 점은 액정시료의 전단속도에 대한 전단 응력은 증가와 감소에서 틱소트로피와 다일레턴시 현상을 보여 hysteresis loop를 나타내고 있다는 점이 다. sodium bis-(2-ethylhexyl)sulfosuccinate-water 라멜라 액정은 작은 전단속도에서는 약한 젤 현상 을 보이지만 응력이 한계 응력 이상에서는 비 선형 점탄성 성질을 나타낸다. 전단속도 감소에서 분산계 는 전단속도가 증가할 때 측정된 값 보다는 큰 구조변화와 전단응력을 유지하고 있다.

4-isopropyl-2,6-bis(1-phenylethyl)aniline 1 (KTH13-AD1) is a metabolite of Cordyceps bassiana that has been traditionally used to treat various inflammatory disease. Even though it has the magnificent pharmaceutical potential, there was not much understanding on its anti-inflammatory actions. Therefore, this study was aimed to determine the anti-inflammatory effects of KTH13-AD1. We found that KTH13-AD1 suppressed nitric oxide (NO) and reactive oxygen species (ROS) production in lipopolysaccharide (LPS)- or sodium nitroprusside (SNP)-treated macrophages (RAW264.7 cells). Similarly, mRNA expression of inducible NO synthase (iNOS) and tumor necrosis factor-a (TNF-a) analyzed by RT-PCR and real-time PCR was also reduced by this compound. Interestingly, KTH13-AD1 also strongly diminished the levels of NF-kB-mediated luciferase activities and nuclear translocated NF-kB family proteins. In agreement with these, KTH13-AD1 suppressed the upstream signaling pathway for NF-kB activation including IkBa, IKKa/b, AKT, p85/PI3K and Src in time- and dose-dependent manners. Therefore, these results strongly suggest that KTH13-AD1 has a strong anti-inflammatory activity via suppression of the NF-kB signaling pathway.

목 적: 본 연구는 습윤성 물질을 포함한 기본적인 하이드로젤 콘택트렌즈 재료에 silicon 2,3-naphthalocyanine bis(trihexylsilyloxide)(SiNc)를 첨가하여 친수성 콘택트렌즈의 물리적 및 광학 특성을 측정하였다. 또한 제조된 고분자의 자외선 투과율과 접촉각을 측정하여 SiNc의 안의료용 렌즈 소재로의 활용도, 특히 습윤성을 향상시키는 안의료용 고분자로서의 활용도에 대해 알아보았다. 방 법: 친수성 하이드로젤 렌즈 제조를 위해 2-hydroxyethyl methacrylate (HEMA), N-vinyl-2-pyrrolidone (NVP), methylmethacrylate (MMA), 교차결합제인 ethylene glycol dimethacrylate (EGDMA) 그리고 개시제인 azobisisobutyronitrile (AIBN)을 사용하여 공중합 하였다. 또한, SiNc는 첨가제로 사용되었다. 중합 후 제조된 콘택트렌즈 재료의 함수율, 굴절률, 접촉각 및 분광투과율 등의 물리적 특성을 측정하였다. 결 과: 생성된 하이드로젤 렌즈 고분자의 물리적 특성을 측정한 결과, 함수율 38.12 ~ 37.98%, 굴절률 1.434 ~ 1.435, 접촉각 34.33 ~ 62.91°그리고 분광투과율의 경우 34.6 ~ 91.0%의 범위로 나타났다. 또한 SiNc를 첨가한 조합에서 자외선 투과율이 크게 감소하였으며, 습윤성은 증가하였다. 결 론: 습윤성 물질 및 SiNc를 첨가하여 제조된 안의료용 고분자 재료는 자외선 차단 및 습윤성이 우수한 하이드로젤 콘택트렌즈를 제조하기 위한 재료로 유용하게 사용될 것으로 기대된다.

A series of noble poly(amide-imide)s and copoly(amide-imide)s bearing 1,2-bis(4-phenoxy)benzene units were synthesized by the direct polycondensation of 1,2-bis(4-trimellitimidophenoxy)benzene[1,2-PTPB] with a combination of commercially available aromatic diamines and diacids such as m-phenylene diamine, p-phenylene diamine(PPD), isophthalic acid and terephthalic acid(TA) in N-methyl-2-pyrrolidone(NMP) using triphenyl phosphite and pyridine as a condensing agent in the presence of dehydrating agent (CaCl2). The resulting polymers had inherent viscosities in the range of 0.37~0.78 dL/g and most of them were soluble m common organic solvents including NMP, dimethylacetamide, dimethylsulfoxide, dimethylformamide, and m-cresol. Wide-angle X-ray diffractograms revealed that the copoly(amide-imide) derived from PPD with mixed acids of 1,2-BTPB and TA, showed crystalline nature, whereas all of the other polymers were found to be amorphous. The glass transition temperatures of the polymers occurred over the temperature range of 270~323℃ in their differential scanning calorimetry curves and their 10% weight loss temperature, determined by thermogravimetric analysis in air and nitrogen atmosphere, were in the range 465~535℃, 500~550℃, respectively, indicating their good thermal stability.

The Maillard Reaction Products (MRPs) such as Glucose-tyrosine (Glu-Tyr) and Xylose-arginine (Xyl-Arg) have antioxidant, antimutagenic, and antibacterial effects. However, to date, still little is known about the other biological effects of the MRPs. In this study, we investigated whether the fructose-tyrosine MRP, 2,4-bis(p-hydroxyphenyl)-2-butenal (Fru-Tyr), could modulate cell cycle progression and NF-κB activity, and thereby induce apoptotic cell death of colon cancer cells. Treatment with different concentrations (10-40 μg/ ml) of Fru-Tyr for 24 h inhibited colon cancer cell (SW620 and HCT116) growth followed by induction of G₂/M phase cell cycle arrest and apoptosis in a dose-dependent manner. We also found that Fru-Tyr suppressed tumor necrosis factor-alpha (TNF-α)-induced NF-κB transcriptional activity. Moreover, Fru-Tyr induced the expression of apoptotic gene, cleaved caspse-3. These results suggest that Fru-Tyr inhibited colon cancer cell growth through induction of G₂/M phase cell cycle arrest and apoptotic cell death by modulating of NF-κB.

A group of new N,N-bis(5-acetylpyridin-2-yl)phenylamine derivatives was synthesized in good yield applying an optimized Buchwald-Hartwig amination protocol. The synthesized compounds showed UV absorption maxima in the range of 320-360 nm, and showed good luminescence at dilute concentrations in the blue region of the spectra (in the range of 480-497 nm). They showed also a bathochromic shift associating the increase in solvent polarity. The synthesized compounds could be investigated for use in OLEDs or as potential monomers for PLEDs.

1.2-bis(aminoacyl)hydrazine derivatives and dipeptides were synthesized by conventional peptide synthesis procedures. Their antioxidant activity were inverstigated by over-storage test using corn oil as substrte. 1.2-bis (aminoacyl) hydrazine derivatives and dipetides containing hydrophobic side chain amino acid showed higher antioxidant activity. A free N-terminal amino group was also found to be important for the appearance of antioxidant activity. 1.2-bis (aminoacyl) hydrazine derivatives showed higher antioxidant activity than dipeptides. Antimicrobial activites of dipeptides and 1.2-bis (aminoacyl) hydrazine derivatives were also examined by the paper disc method. All of these compounds had shown no antimicrobial activity.