Bovine coat color is decided by the melanocortin receptor 1 (MC1R) genotype mutation and melanogenesis. Specially, in the various cattle breeds, dominant black coat color is expressed by dominant genotype of ED, red or brown is expressed in the frame shift mutation of recessive homozygous e by base pair deletion and wild type of E+ is expressed in various coat colors. However, not very well known about the effected of MC1R genotype mutation on the coat color through family lines in KBC. Therefore, this study were to investigate effect of MC1R genotype mutation on the coat color, and to suggest mating breed system in accordance with of MC1R genotype for increased on brindle coat color appearance. Parents (sire 2 heads and dam 3 heads) and offspring (total : 54 heads) from crossbreeding in KBC family line with the MC1R genotype and phenotype records were selected as experimental animals. The relationship between melanocortin 1 receptor (MC1R) genotypes expression verified by PCR-RFLP, and brindle coat color appearance to the family line of the cross mating breed from MC1R genotype pattern was determined. As a result, 4MC1R genetic variations, E+/E+ (sire 1), E+/e (sire 2 and dam 3), E+/e with 4 bands of 174, 207 and 328 bp (dam 1) and E+/e with 3 bands of 174, 207, 328 and 535 bp (dam 2) from parents (sire and dam) of KBC. However, 3 genetic variations, e/e (24%), E+/E+ (22%) and E+/e (56%) were identified in offspring. Also, brindle coat color expressrated was the e/e with the 0%, E+/E+ with 67% and E+/e with 77% from MC1R genotype in offspring on the cross mating of KBC. Furthermore, when the sire had E+/e genotype and the dam had E+/E+ with the 3 bands or E+/e genotype, and both had whole body-brindle coat color, 62% of the offspring had whole body-brindle coat color. Therefore, the seresults, the mating system from MC1R genotype patterns of the sires (E+/e) and dams (E+/E+ with the 3 bands or E+/e) with brindle coat color may have the highest whole body-brindle coat color expression in their offspring.

The environment in which a given genotype is grown may influence its grain quality characteristics. When varieties are ~times evaluated over numerous environments, a variety environment interaction usually is observed, but the relative magnitude of environmental(E), genetic(G), and G ~times E effects on quality is unclear. In order to determine relative contribution of genotype, environment, and G ~times E interaction to the variations observed in grain quality characteristics, 18 Korean wheat cultivars and experimental lines were evaluated in two environments in 1998 and 1999. Correlation coefficients between grain quality and agronomic characteristics were also estimated. The analysis of variance for the optical density obtained by reaction bet- ween gliadin and anti-gliadin polyclonal antibody (AGPab) indicated that gliadin content measured by Enzyme-Linked Immunosorbent Assay(ELISA) was significantly in- fluenced by environment and cultivar differences. The significant differences of year and year ~times location were also found. The ratio of the variances associated with environmental effects to the variances associated with genetic effect gave relatively greater influence of environmental factor on gliadin content. The different protein content from same genotype grown in different environment might be associated with degree of storage protein accumulations. Significant relationships between ELISA and protein content, yield, ten spike weight, and ten spike number were detected. Polyphenol oxidase (PPO) activity was significantly influenced by year, location, cultivar and year ~times location. The variance in grain PPO activities among growing years appeared larger than the variation produced by the cultivar examined. This suggested that the growing environment contributed more to variability in grain PPO concentration.

콩 유전자형간 상호접목을 통하여 지상부 생육과 콩깍지에 대한 종실건물중 비율에 미치는 영향을 비교 검토하고자, 꼬투리의 비대가 충실한 백운콩과 수원 158호, 꼬투리 크기에 비하여 종실건물축적이 불량한 수집검정콩 강릉재래 및 금릉재래를 공시하여, 자가접목 및 4개 유전자형간 상호접목을 한 후 이식재배시험한 결과를 요약하면 다음과 같다. 1. 지상부의 생육정도와 콩깍지 및 종실건물중은 지상부 유전자형간 유의적인 차이가 인정되었으나, 지하부 유전자형간 차이가 없었다. 2. 백립중 및 꼬투리에 대한 종실건물중 비율도 지상부 유전자형에 의하여 결정되기 때문에, 상대적으로 양분흡수와 밀접한 관련이 있는 지하부 유전자형과는 관계가 적었다. 3. 접목된 개체들의 콩깍지 건물중과 콩깍지에 대한 종실건물 비율은 유의적인 부의 상관관계를 나타내고 있어, 콩깍지 건물중이 클수록 콩깍지에 대한 종실건물 비율이 낮아지는 경향이었다.