Leaf area is critical for crop light interception, and thereby has a substantial influence on crop yield. This experiment was conducted to characterize the development of soybean [Glycine max (L.) Merr.] leaf area. Plastochron index and leaf relative growth rate of Jackson was contrasted with the PI416937, which also has demonstrated tolerance to drought. First, plastochron ratio (PR) and plastochron index (PI) were evaluated in greenhouse to compare the leaf growth rate between two genotypes under well-watered condition. There was reasonable constancy of PR between two genotypes. The PR means of Jackson and PI416937 were 0.41 and 0.44, respectively. A fairly smooth increase of PI during vegetative stage was observed. Second, the relative growth rates were graphed against leaf area, normalized with respect to final leaf area, under well-watered and water-deficit conditions. Leaf growth was sustained longer in well-watered condition than water-deficit condition and there was a sizable proportion of leaves which was ceased earlier their growth in water-deficit condition compared to well-watered condition. The leaf relative growth rate of Jackson until leaves had completed at 45% of their growth during water deficit period was higher than that of PI416937.
Water deficit is the primary constraint of soybean [Glycine max (L.) Merr.] yield, and a physiological understanding of processes affected by water deficit is a key step in identifying and improving drought tolerance in soybean. The objectives of this research were to evaluate biomass and nitrogen accumulation patterns and water use efficiency (WUE) as possible mechanisms associated with the drought tolerance of Jackson. Biomass accumulation of Jackson was contrasted with the PI416937, which also has demonstrated tolerance to drought. For water-deficit treatment, total biomass accumulation was negligible for PI416937, but biomass accumulation continued at approximately 64 % of the well-watered treatment of Jackson. Transpirational losses for Jackson and PI416937 were approximately the same for the water-deficit treatment, indicating that Jackson had superior WUE. Isotopic discrimination of 13 C relative to 12 C also indicated that Jackson had higher WUE. Results indicated that increased WUE for Jackson under water deficit showed it was tolerant to drought rather than had an avoidance mechanism.
In order to develop an appropriate cultural practice for a newly bred 2-rowed, waxy, naked barley cultivar 'Duwonchapssalbori' in the southern region, 2 varieties, 3 seeding dates, 4 seeding rates were designed with 2 replications. The heading and maturity dates of 'Duwonchapssalbori' were' earlier than 'Saessalbori by 2-5 and 4 days, respectively. Culm length of 'Duwonchapssalbori' was 60 to 66cm with lodging resistance. Grain yields of both varieties were 4.28-4.33 ton/ha in the plot of Oct. 20 seeding, 4.11-4.18 ton in the plot of Oct. 28 seeding, and 3.7-13.89 ton/ha in the Nov. 5 seeding. Grain yield with different seeding rates highly significantly fitted with secondary polynomial equations in the plots of Oct. 20 and Oct. 28 seedings (R2 =0.84-0.85), showing the greatest yield with seeding rate of 140kg per ha. However, the grain yield with various seeding rates in the plot of Nov. 5 seeding showed simple linear regression (R2 =0.71), showing the seeding rate over 160kg in the case of later seeding to be optimal. Number of spikes per m2 significantly contributed to grain yield, and fitted to simple linear regression (R2 =0.881-0.891), suggesting that plenty of early stands should be established at early seedling stage for high grain yield. Young spikes of 'Duwonchapssalbori' in the plot of Oct. 20 seeding elongated with linear increments since Feb. 10 (6.7mm), and showed rapid increment since March 7 (15.1mm). However, elongation of young spikes of 'Saessalbori' began from March 15 with slow increments. From these studies, the optimum seeding date and seeding rate in 'Duwonchapssalbori' were Oct. 20-28 and 140kg per ha for high grain yield in the southern regions.
Viviparous germination causes yield loss and quality deterioration of rice. This study was conducted to investigate varietal differences of the viviparous germination with different days after heading (DAH) and different temperatures. In the laboratory examination, the averaged germination rate of all varieties at 45DAH and at 25DAH was 79.9%, and 27.5% under the incubation at the temperature of 30~circC /20~circC (day/night) for 12 days. Andabyeo, Da- sanbyeo, and Nonganbyeo showed the lowest viviparous germination rates among the tested varieties. The shoot length of the viviparous germination measured 12days after incubation at 30/20~circC ranged from 21 to 53mm, indicating significant deterioration of rice quality. In the field test, the averaged viviparous germination rates of rice varieties at 25, 35, 45DAH with the underwater conditions for 4 days were 2.2, 6.2 and 9.2%, respectively, while their rates at 12 day after underwater conditions increased to 17.6, 44.2 and 43.8%, respectively. A variety that showed the highest viviparous germination rate at 25 and 35 and 45DAH was Heukjinjubyeo. When standing rice panicles without lodging were examined after consecutive raining for 7 days Juanbyeo showed the highest viviparous germination (45.5%), followed by Odaebyeo (16.0%), Jinbubyeo (14.5 %), Bongkwangbyeo (14.2 %) and Obongbyeo (12.6%). The viviparous germination of rice was greatly affected by variety, days after heading and temperature settings. Bongkwangbyeo, Juanbyeo, Dongjinbyeo, Hwasunchalbyeo, Naepoongbyeo and Heukjinjubyeo were classified as the most susceptible varieties to the viviparous germination in the field conditions.
This study was carried out to obtain the basic information for breeding cold-tolerant rice varieties with high yield-productivity through wide crosses between indica and japonica rice. Genetic analysis was conducted using 55 F1 s obtained from half-diallel crosses among eleven cultivars of various origin including indica and japonica rice. Screening for cold tolerance was done with cold-water irrigation after transplanting until ripening stage. Both general combining ability (GCA) and specific combining ability (SCA) effects were highly significant in all characters associated with dry matter accumulation at 30 and 50days after cold-water irrigation (DAC). The variance of GCA was much larger than that of SCA in plant height, shoot dry weight per plant (DWP), crop growth rate (CGR) and cold-water response index (CRI) of these characters except CRI of shoot dry weight per plant. The DWP, CGR and CRI of these characters of Gaochan 102, Tong88-7 and TR22183 were markedly higher than those of the others. GCA effects of these varieties on DWP, CGR and their CRI were also higher than those of the others, indicating that they are useful as promising parents for breeding cold-tolerant varieties. Analysis of genetic parameters for 11~times 11 half-diallel F1 s revealed that inter-locus gene interaction were concerned in the expression of plant height at 50 DAC, CRI of DWP at 50 DAC, and CRI of CGR, and that intra-locus gene interaction for plant height and the other characters were partial dominance and over-dominance, respectively. Narrow-sense heritability (h2 N ) was the highest in plant height as 0.729, and the lowest in CRI of DWP at 30 DAC as 0.048, suggesting that selection for cold tolerance will be quite effective in case that the selection criterion is the performance itself.f.
The in vitro culture of rice panicles is a culturing technique only panicle without other organs in culture solution containing organic substance, so that would be useful to study how assimilate supply affects grain development and maturation. To find the optimum stage for in vitro culture, rice panicles grown in greenhouse were sampled periodically after anthesis and cultured in nutrient medium. The panicles older than 1 weeks after anthesis had produced normal grains. Grain-filling was apparently dependent upon sucrose concentration (8-12 %) in medium, but not affected by nitrogen concentration supplied with glutamine. As far as rice panicle was supplied with sucrose and N in nutrient medium, grains continued accumulation of dry matter and maturation regardless to light condition. Considerably, grain-filling was improved when panicles were positioned horizontally inside flask, so that each grain was partially submerged to nutrient medium.
The environment in which a given genotype is grown may influence its grain quality characteristics. When varieties are ~times evaluated over numerous environments, a variety environment interaction usually is observed, but the relative magnitude of environmental(E), genetic(G), and G ~times E effects on quality is unclear. In order to determine relative contribution of genotype, environment, and G ~times E interaction to the variations observed in grain quality characteristics, 18 Korean wheat cultivars and experimental lines were evaluated in two environments in 1998 and 1999. Correlation coefficients between grain quality and agronomic characteristics were also estimated. The analysis of variance for the optical density obtained by reaction bet- ween gliadin and anti-gliadin polyclonal antibody (AGPab) indicated that gliadin content measured by Enzyme-Linked Immunosorbent Assay(ELISA) was significantly in- fluenced by environment and cultivar differences. The significant differences of year and year ~times location were also found. The ratio of the variances associated with environmental effects to the variances associated with genetic effect gave relatively greater influence of environmental factor on gliadin content. The different protein content from same genotype grown in different environment might be associated with degree of storage protein accumulations. Significant relationships between ELISA and protein content, yield, ten spike weight, and ten spike number were detected. Polyphenol oxidase (PPO) activity was significantly influenced by year, location, cultivar and year ~times location. The variance in grain PPO activities among growing years appeared larger than the variation produced by the cultivar examined. This suggested that the growing environment contributed more to variability in grain PPO concentration.
This study was conducted to assess the allelopathic potentiality of Korea traditional seventy-nine rice (Oryza sativa L.) cultivars on barnyardgrass (Echinochloa crus-galli P. Beauv. var. oryzicola Ohwi) and to identify possible allelopathic compounds from selected rice cultivars, such as Seogandodobyeo, Huadobyeo and Heugbalbyeo. In the straw mixture, Seogandodobyeo showed the greatest inhibition (67.07%) on total emergence percentage. The greatest inhibition on total seedling length and dry weight of barnyardgrass occurred in Huadobyeo (58.32 %) and Heugbalbyeo (81.20%), respectively. An HPLC analysis with nine standard compounds showed that the concentrations and compositions of allelopathic compound depend upon the cultivars. Four compounds including Ο-coumaric acid in Seogandodobyeo extracts, four compounds including p-coumaric acid in Huadobyeo extracts, and seven compounds including Ο-coumaric acid in Heugbalbyeo were detected, respectively. Ο-coumaric acid (0.97 mg/g) in Seogandodobyeo, p-coumaric acid (0.92mg/g) in Huadobyeo and Ο-coumaric acid (1.02 mg/g) in Heugbalbyeo was detected as the highest amounts, respectively. The preliminary identification by HPLC analysis resulted in peaks with retention times close to those of standards which were confirmed with EI/MS. The mass spectra of p-coumaric, and Ο-coumaric acids showed molecular ions (m/z) at 164 and 164, and their base peaks (m/z) at 164 and 118, respectively.
The elongation of mesocotyl and coleoptile plays important roles in the seedling emergence and stand establishment of dry direct-seeded rice. Experiments were carried out to elucidate the effects of seed-presoaking treatments of GA3 and some inorganic salts on the mesocotyl, and coleoptile elongation of rice. Seed-soaked GA3 promoted the elongation of mesocotyl, but little effect on the coleoptile elongation. The stimulation effects of GA3 were found to be enhanced by addition of CaCl2 However, the sole treatment of CaCl2 showed no stimulating effect on the mesocotyl and coleoptile elongation. Mesocotyl elongation was most prominent in the combined treatments of 50ppm GA3 with 100 mM CaCl2 . The synergistic effects of GA3 and CaCl2 on mesocotyl elongation varied with varietal groups. The stimulating effects of GA3 were enhanced significantly by the addition of CaCl2 in japonica varieties, Dongjinbyeo, Ilpumbyeo and Milyang 95, and tall indica variety, Labelle, but not in semidwarf Tongil type varieties, Tongilbyeo, Milyang 23, and Nampungbyeo, and semi-dwarf indica, Short Labelle. The promoting effects of GA3 on the mesocotyl elongation were decreased in proportion to the lowered osmotic potential by PEG 6000 on the contrary to CaCl2 This implies that the synergistic effects of CaCl2 with GA3 on mesocotyl elongation was not caused by osmotic potential lowered by CaCl2 addition but by the salt itself. Salts such as Ca(NO3 )2 , MgCl2 BaCl2 , NaCl, KCl and KNO3 showed the synergistic effects with GA3 on mesocotyl elongation as well. The degree of synergistic effects showed no differences among salts tested, implying that there is no specificity of ions constituting the salts.
Germination of sweet and super sweet corn is lower than normal corn due to the higher sugar and lower starch contents of kernels. Sweet corn seeds are easily deteriorated in the field under the unfavorable condition, therefore it is important to identify the optimal harvesting time for seed production. This trial was conducted to investigate the responses of germination percentage of shrunken-2(sh2), brittle(bt), sugary(su), and sugary enhancer(se) hybrids in relation to harvesting dates. Eight hybrids of four different gene sweet corns were harvested at 15, 20, 25, 30, 35, 40, 45, and 50 days after silking(DAS). Germination test was performed using paper towel method. Mean germination percentages across eight hybrids showed the highest value at 45 DAS. There were significant differences among genes and within gene for germination. Shrunken-2 hybrid Mecca was higher than su hybrids for germination, indicating that sh2 would not be poorer than su Late harvesting beyond the optimal harvesting date might not be desirable because of more lodging and ear rots. Theoretical optimal harvesting date estimated from the regression equation was 40.9 DAS, however, practical date for harvesting would be a few days later than the estimated date if seedling vigor might be considered. Kernel dry weight per ear showed similar response to germination. Regression equation showed the highest kernel dry weight at 40.7 DAS. Significant correlations between kernel dry weight and germination were observed, impling that kernel dry matter accumulation would be an important factor for germination.
In nature, plant diseases, insects and parasites (hereafter called as "pest") must be co-survived. The most common expression of co-survival of a host crop to the pest can be tolerance. With tolerance, chemical uses can be minimized and it protects environment and sustains host productivity and the minimum pest survival. Tolerance can be applicable in all living organisms including crop plants, lifestocks and even human beings. Tolerant system controls pest about 90 to 95% (this pest control system often be called as horizontal or partial resistance), while the use of chemicals or selection of high resistance controls pest 100% (the most expression of this control system is vertical resistance or true resistance). Controlling or eliminating the pests by either chemicals or vertical resistance create new problems in nature and destroy the co-survial balance of pest and host. Controlling pests through tolerance can only permit co-survive of pests and hosts. Tolerance is durable and environmentally-friend. Crop cultivars based on tolerance system are different from those developed by genetically modified organism (GMO) system. The former stabilizes genetic balance of a pest and a host crop in nature while the latter destabilizes the genetic balance due to 100% control. For three decades, the author has implemented the tolerance system in breeding maize cultivars against various pests in both tropical and temperate environments. Parasitic weed Striga species known as the greatest biological problem in agriculture has even been controlled through this system. The final effect of the tolerance can be an integrated genetic pest management (IGPM) without any chemical uses and it makes co-survival of pests in nature.in nature.