Drought stress has detrimental effects on the seedling development, vegetative/ reproductive growth, photosynthesis, root proliferation, anthesis, anthesis-silking interval (ASI), pollination and grain yield in maize. Typically, two weeks before silking through pollination are an important time in maize life. Here we reviewed the effects of drought stress on growth, physiological/ molecular researches for drought tolerance, and breeding to genomics in maize. Drought stress during kernel development increases leaf dying and lodging, decreases grain filling period and grain yield. Physiological factors of drought stress/ effects are water content, water deficits, and water potential. Nowdays molecular marker assisted breeding method is becoming increasingly useful in the improvement of new germplasm with drought stress tolerance.
Even though phosphorus (P) is essential element for plant growth and development, it is not enough for crop production in soil. To breed more P deficient tolerance rice, screening and selection in rice population is needed. We tried to develop more simple and rough screening method for breeding of P deficient tolerance rice. In P deficient condition, tiller number was dramatically decreased among yield components in rice. Though this result, we confirmed tiller number could be the best marker in screening of P deficient tolerance rice. 480 rice genetic resources were cultivated in rice bed tray filled with P deficient soil for four weeks and each dry weight was measured. Among them, the 55 kinds of genetic resource were selected then cultivated in paddy field with 3 fertilizer conditions. Plant dry weight and tiller number in ripening stage were shown significant difference according to P condition. Plant dry weight and tiller number in ripening stage was highly correlated especially in P deficient condition. Furthermore, the tiller number in ripening stage and plant dry weight in rough screening were shown high degree correlation. Though these results, we might expect measuring of plant dry weight after cultivation in rice bed tray filled with P deficient soil could be a simple and effective screening method in selection of P deficient tolerance rice.
Rural Development Administration has recently developed mid-late maturing rice cultivars with high quality. This study was conducted to select suitable mid-late maturing rice cultivar in major cultivation areas of Gangwon Province among domestic breeding cultivars for 2 years from 2013 to 2014. The average air temperature during the experiment in 2013 and 2014 was higher 0.6~1.1°C in Chuncheon, 1.0~ 1.3°C in Gangreung, and 0.1~0.7°C in Cheolwon than normal year. Precipitation in ripening period was higher 52.5 mm in 2013, but lower 176.4 mm in 2014 than normal year in Chuncheon, lower 103.1 mm in 2013 and higher 42.9 mm in 2014 in Gangreung, lower 225.9~322.7 mm in Cheolwon. Duration of sunshine in ripening period was higher 142 hours in Chuncheon, 108 hours in Gangreung, and 94 hours in Cheolwon than normal year in 2013, higher 20~21 hours in Chuncheon and Gangreung, and 82 hours in Cheolwon than normal year in 2014. Milled rice yield of ‘Samgwangbyeo’ and ‘Daebobyeo’ was 594 kg per 10a, and 578 kg for ‘Jinsumibyeo’ in Chuncheon in plain area. For Gangreung in east coastal area, the yield of ‘Daebobyeo’ was 555 kg per 10a, ‘Hopumbyeo’ was 554 kg, and ‘Chilbobyeo’ was 546 kg. For Cheolwon in mid-mountainous area, the yield was 504 kg per 10a for ‘Daebobyeo’ and 489 kg for ‘Haiamibyeo’. But there was no difference in milled rice yield among cultivars tested in the study at each area. Head rice ratio of ‘Haiamibyeo’, ‘Jinsumibyeo’, and ‘Mipumbyeo’ in Chuncheon was 94.2~95.6% higher than the other cultivars. In Gangreung head rice ratio of ‘Hopumbyeo’, ‘Haiamibyeo’, and ‘Samgwangbyeo’ were 85.2~88.3% but there was no difference among cultivars except ‘Gopumbyeo’ and broken rice ratio of ‘Haiamibyeo’, ‘Samgwangbyeo’, and ‘Mipumbyeo’ were low as 7.5~8.5% and palatability of ‘Mipumbyeo’ was higher than the other cultivars. Head rice ratio of ‘Daebobyeo’ was the highest as 89.2% and there was no difference in broken and chalky rice ratio and palatability among cultivars in Cheolwon. Considering rice yield and quality in major cultivation areas of Gangwon Province, suitable mid-late maturing rice cultivars were ‘Samgwangbyeo’, ‘Haiamibyeo’, and ‘Jinsumibyeo’ in Chuncheon, ‘Daebobyeo’ and ‘Chilbobyeo’ in Gangreung, and ‘Daebobyeo’ and ‘Haiamibyeo’ in Cheolwon. The results obtained in the study imply that the selected cultivars with high yield and quality and suitability to Chuncheon, Gangreung, and Cheolwon, respectively could be recommended to rice cultivating farmers in the regions with high priority.
Rice (Oryza sativa L.) ratooning is the production of a second rice crop from the stubble left behind after the main crop harvest. The objectives of this study were to evaluate the effects of main-crop stubble height, irrigation and fertilization on ratoon grain yield. Main crop ‘Jinbuol’ rice cultivar was harvested to leave with 10, 20, 30, or 40 cm stubble height. When the main crop stubble was harvested with 10 cm height, ratoon rice grain yield was increased by 2,810 kg/ha. Irrigation on stubbles after main crop harvest did not affect the ratoon crop yield and rice quality. The results showed a large variation in the ratoon performance by fertilizer application methods. Top-dressed nitrogen fertilizer on the stubble after harvest caused increase in panicle production and higher maturity rate. However, there was no significant difference in protein content, amylose content of milled rice and cooked rice characters between plots managed with and without nitrogen fertilizer.
‘Superhongmi’ is a new rice variety with reddish brown color developed from a crossing a high cyanidin 3-glucoside (C3G) content beeeds including Heugjinju and Suwon 425 and a big grain size breed such as Daeribbyeo. This variety heads on Sep. 5 and has 86.7 cm culm length. The fertility ratio of Superhongmi was about 81.7% and weight of 1,000-grain was about 26.8 g. The 1,000 grain weight of Superhongmi was heavier than that of Superjami. The total essential amino acid content of Superhongmi was lower than those of others. However, Superhongmi was high in tyrosine and arginine. Total polyphenol content of Superhongmi was 2 times higher than that of Heugjinju and flavonoid content was 8 times higher than that of Dongjinbyeo. In addition, hydroxy radical scavenging activity of Superhongmi was significantly higher than that of Dongjinbyeo. However, significant differences were not found in the newly breed colored rice. These results suggest that Superhongmi variety has very high value as a source of various functional food as well as stable food.
This research analyzed the characteristics of varieties of rice such as a harvest time and yield, and to select a proper variety to product green rice according to a conventional planting culture and a late planting culture. The most proper harvest time of rice, in general, was 15 to 25 days after heading to product the green rice. Sinsunchal among glutinous rice varieties and Chilbo among nonglutinous rice varieties showed the most amount harvest at 25 days after heading, which was a limit harvest time for the whole green rice production in overall rice varieties. The amount of green rice according to transplanting times, the May 30 th transplanting was more than the June 20 th . The yield of green rice harvested at 15~25 days after heading was varied according to varieties. Proper glutinous varieties for green rice production were Dongjinchal, Sangdongchal, Boseokchal, and Sinsunchal, in order of listed, which were transplanted at the May 30 th . Meanwhile, Boseokchal, Backokchal, and Sinsunchal, in order of listed, were proper varieties for green rice production, which were transplanted at the June 20 th . In nonglutinous rice, Samkwang, Nunbora, and Chilbo, which were transplanted at the May 30 th , were proper varieties for green rice production. Hwanggeomnodeul, Hopum, and Chilbo, which were transplanted at the June 20 th , were proper varieties for green rice production.
Developing rice lines with various amylose contents is necessary to diverse usages of rice in terms of raw materials for processed food production, and thereby to promote rice consumption in Korea. A rice mutant line, ‘Namil(SA)-dull1’ was established through sodium azide mutagenesis on ‘Namil’, a non-glutinous Korean Japonica rice cultivar. Namil(SA)-dull1’ had dull endosperm characteristics and the evaluated amylose content was 12.2%. A total of 94 F2 progenies from a cross between ‘Namil(SA)-dull1’ and ‘Milyang23’, a non-glutinous Tongil-type rice cultivar, was used for genetic studies on the endosperm amylose content. Association analyses, between marker genotypes of 53 SSR anchor markers and evaluated amylose contents of each 94 F2:3 seeds, initially localized rice chromosome 6 as the harboring place for the modified allele(s) directing low amylose content of ‘Namil(SA)-dull1’. By increasing SSR marker density on the putative chromosomal region followed by association analyses, the target region was narrowed down 0.94 Mbp segment, expanding from 28.95 Mbp to 29.89 Mbp, on rice chromosome 6 pseudomolecule. Among the SSR loci, RM7555 explained 84.2% of total variation of amylose contents in the F2 population. Further physical mapping on the target region directing low amylose content of ‘Namil(SA)-dull1’ would increase the breeding efficiency in developing promising rice cultivars with various endosperm characteristics.
This study was conducted to investigate the mixed seeding of 5 forage barley varieties (youngyang, yuyeon, dami, wooho, soman) with 1 malting barley variety (hopum). Yield of the mixed seeding was compared with each single seeding and variety combinations. Yield of mixed seeding combination was higher than each single seeding. In mixed seeding of ‘Youngyang’ and ‘Hopum’ (50% and 50% ratio), ‘Yuyeon’ and ‘Hopum’ (70% and 30% ratio), ‘Dami’ and ‘Hopum’ (50% and 50% ratio), ‘Wooho’ and ‘Hopum’ (50% and 50% ratio) were higher yield of dry weight than yield of other mixed ratios. Most of all, mixed seeding of ‘Dami’ and ‘Hopum’ were high yield mixed seeding (ratios of 50% each other). In generally, the yields of barley mixed seeding with malting barley ‘Hopum’ were high production. The forage quality of mixed seeding combination was higher than any each single seeding. ‘Youyeon’ 50% and ‘Hopum’ 50% ratio was more higher
This study was carried out to find relationships of genetic distance and heterosis expression degree in the developed CNU waxy corn lines. The used material were developed in Corn Breeding Laboratory, Coll. of Agri. & Life Sci., CNU. Total 10 primers used for SSR maker analysis. In genetic distance, the used lines divided into four groups; A group has two lines, B group has one line, C group has ten lines and D group has eleven lines, respectively. Among used lines, CNU427 and CNU588 were very closed as a 74, while CNU451 and CNU429 were remote as a 40 inbred coefficient, respectively. While heterosis degree were very variable not only hybrids but also cross parents. Average heterosis of most cross-parent was high in plant height, ear height, flowering day and ear length. Especially, CNU H09-23 hybrid was high as 79.4% than other hybrids. Here, we gained the fact has closely relationship between genetic distance and heterosis.
This study was carried out to compare botanical and amylogram characteristics including table quality in waxy corn kernel. The used materials in this experiment were producted and evulated at Corn Breeding Laboratory, Coll. of Agri. & Life Sci., in CNU. In botanical characteristics CNU H09-26 among used hybrids was highest in stem height as 228.5 cm, but that of CNU H09-30 was lowest. Ear height was highest in CNU H09-23 as 78.2 cm, but that of CNU H09-30 was lowest. Ear length among hybrids were also variable as 21.2 cm to 10.8 cm. in amylogram analysis CNU H09-23 hybrid was lowest in pasting temperature, while break down of this hybrid was highest These results appeared highly in table quality. Accordingly we thought that this hybrid will be adapted as a leading variety for edible waxy corn.
We examined the effects of light colors (wavelength) and light quantities on the yield ratio and quality of cultivated cowpea sprouts. All light colors resulted in a lower cowpea sprout yield ratio compared to the untreated condition (darkness), but were similar in hard seed ratio. All light colors promoted the growth of the epicotyl and root when compared to the untreated condition, but limited the growth of the hypocotyl. White light (458 nm) significantly improved grade by increasing the lightness of the cotyledon and the hypocotyl and the yellowness of the hypocotyl. The Fe content of cowpea sprouts was higher in those grown under red light (632 nm), and the total amino acid content was higher for those grown under yellow light (560 nm), white light (458 nm), and blue light (460 nm) compared to plants grown in the untreated condition (darkness). The yield ratio of cowpea sprouts was lower in the yellow light condition (560 nm) at lower light quantity, but no differences were observed at other light colors and quantities. The lightness and yellowness of cowpea sprouts was higher in the yellow light (560 nm) and red light (632 nm) at lower light quantity, redness was lower. No significant differences were observed in the content of normal and inorganic components according to the light quantities of each light color, except that Fe content was higher in sprouts grown under red light (632 nm) as light quantity increased. Total amino acid content was slightly higher in sprouts grown under white light (458 nm) and blue light (460 nm) as light quantity increased.
Thirty-eight Pea (Pisum sativum L.) genotypes were screened to identify varieties to be suitable for sprout. Based on seed yield and sprout qualities such as whole length and sprout yield, five genotypes (PI269803, PI343278, PI343283, PI343300 and PI 343307) were primarily selected as candidates for pea sprouts. In order to determine optimal cultivation condition for pea sprouting, growth characteristics were investigated according to the change of germination temperature and days for sprouting. Whole length and hypocotyl length were observed to increase as a time dependent manner at each tested temperature (20, 23, and 25°C). However, whole length, hypocotyl length, and sprout yield were highly increased at 23°C compared to 20 and 25°C. Especially, PI269803 and PI343300 showed higher sprout yield than the others. In addition, the effect of the change of germination temperature on antioxidant properties was estimated by measuring total phenolic content (TPC) and free radical scavenging activity (DPPH and ABST activity). TPC and DPPH/ABST activities of PI269803 and PI343300 were higher at 23°C than at 20 and 25°C, while antioxidant properties of PI343278 and PI343283 were decreased in a temperaturedependent manner. The results show a high degree of correlation between TPC and antioxidant activities and suggest that the temperature change for pea sprouting could be responsible for antioxidant properties. Taken together, these results provide optimal cultivation conditions for pea sprouting and suggest that PI269803 and PI343300 with high sprout yield and antioxidant properties could be used for pea sprouts.
Small-sized tuber of sweet potato (Ipomoea batatas Lam.) is more preferred by Korean consumers, because it is convenient to eat with hands, and can easily be steamed or roasted in small pan as a healthy snack for a small family. The present study was aimed to investigate the effect of highdensity plantings and harvesting times on production of smallsized tuber (50~200 g). Four varieties (‘Annobeny’, ‘Daeyumi’, ‘Shinzami’, and ‘Yeonhwangmi’) were planted in 2-row zigzag high-density planting (75×30 cm, A; 75×25 cm, B) and 1-row planting (75×25 cm, control) covered with black vinyl film on May 23th, 2014. Marketable yields and small-sized tuber yields were compared between 120- and 150-day harvesting. Vine length and fresh weight per plant were significantly decreased in 2-row planting A and B plots compared to the control at 120 days after planting. Weight of tuber per plant, mean weight of tuber and number of tuber per plant were significantly decreased in 120-day harvesting of A and B plots than in the control. Marketable yields per 10a were increased by 17% (2.4 ton‧10a -1 ) and 8% (2.6 ton‧10a -1 ) in 120-day harvesting of A and B plots compared to the control (2.4 ton‧10a -1 ), and those were largely increased by 29% (4.0 ton‧10a -1 ) and 26% (3.9 ton‧10a -1 ) in 150-day harvesting than in the control (3.1 ton‧10a -1 ). Proportions of small size tuber (50~200 g) were increased by about 65% in 150-day harvesting of A and B plots compared to the control (55.3%). Small-size tuber yield and marketable yield were highly increased in 150-day harvesting of 2-row zigzag high-density planting A (75×30 cm).
This study was conducted to evaluate the cytotoxicity and α-amylase, α-glucosidase, pancreatic lipase inhibition in vitro by different solvent fractions from the roots of Codonopsis lanceolata. The values of IC50 against Calu-6 cell showed a high effect in n-hexane fraction (10.13 μg/mL) whereas DW fraction exhibited the weakest inhibition on cell viability, having an IC50 value of over 1,000 μg/mL. The values of IC50 against HCT-116 cell showed the highest activity in n-BuOH fraction (102.01 μg/mL), followed by n-hexane fraction (145.85 μg/mL), methylene chloride fraction (332.02 μg/mL), ethyl acetate fraction (462.93 μg/mL) and DW fracion (>1,000 μg/mL). α-Amylase inhibitory activity in methylene chloride fraction and ethyl acetate fraction was found to have a higher inhibitory effect with 24.5% and 25.6% than the other fractions. The highest α-glucosidase inhibitory activity was observed from the ethyl acetate fraction extract, while the extract of DW fraction showed the lowest level of inhibitory activity at given experiment concentration. The pancreatic lipase inhibitory activity of C. lanceolata was found to have a higher the effect in ethyl acetate fraction. Inhibition of lipase activity of the ethyl acetate fraction and n-hexane fraction showed a relatively high, while the extract of DW fraction showed the lowest level at given experiment concentration. These results suggested that the roots of C. lanceolata may assist in the potential biological activity on carbohydrate, lipid Inhibitory activity and anticancer activity.
This research carried out to figure out the effect of the green manure crop cultivated at a preparation field and the shading net on the growth, development, and quality of ginseng. Followings are results obtained from the research. Leaf width of ginseng under the shading net of a two-layered blue and two-layered black polythylene net (TBTBPN) was good at rye and hairy vetch cultured group. Leaf length of ginseng under the shading net of a threelayered blue and one-layered black polyethylene net (TBOBPN) was good at barley and hairy vetch cultured group. Meanwhile, leaf width was good at hairy vetch cultured group. Leaf length of ginseng under a blue polyethylene sheet (BPS) was good at a barley and barley + hairy vetch cultured group, but stem length was shorter compare to other shading net cultivations. Root weight of ginseng was good under the shading net of a two-layered blue and two-layered black polyethylene net (TBTBPN) at a rye and hairy vetch cultured group, and was good under the shading net of a three-layered blue and onelayered black polyethylene net (TBOBPN) at a barley + hairy vetch cultured group, but there was no significant difference under blackout screen according to manure crop varieties. Ratio of rusty root was 10.2% at the barley cultured group under the shading net of a two-layered blue and two-layered black polyethylene net (TBTBPN), and was 23.1% at hairy vetch cultured group under shading net of a three-layered blue and one-layered black polyethylene net (TBOBPN). Ratio of rusty root was the lowest at a rye cultured group regardless the shading nets. Content of the ginsenoside was the highest at the rye cultured group under the shading net of two-layered blue and two-layered black polyethylene net (TBTBPN), was the highest at the barley cultured group under the shading net of a three-layered blue and one-layered black polyethylene net (TBOBPN), and was the highest at the rye cultured group under the blackout screen.
Miscanthus has been considered as the most promising bioenergy crop for lignocellulosic biomass production. In Korea, M. sacchariflorus and M. sinensis can be found easily in all regions. It is a great advantage to utilize as important species with respect to genetic and cross-breeding programs materials for creation of novel hybrids. For successful breeding programs, it is important to precisely understand the variability of flowering traits among Miscanthus species as breeding parents materials. In this study, flowering traits were observed daily in 960 germplasms of two Miscanthus species (M. sacchariflorus and M. sinensis) for growing seasons over 2 years. The flowering process was divided into three stages. ST (sprouting time) was recorded when first leaf of the plant emerged on soil. FS1 (flowering stage 1) and FS2 (flowering stage 2) were recorded when flag leaf was firstly observed, and 1 cm of panicle was showing on at least one stem, respectively. For 2013 and 2014, the latest germplasms exerted flag leaf, i.e. September 30 (DOY of FS1 164.1) and September 4 (DOY of FS1 141.0) occurred M. sacchariflorus cv. Geodae 1 and M. sacchariflorus cv. Uram collected from Southern Korea (Jeollanam-do), while Miscanthus germplasms collected from northern Korea (Gyeonggi-do) which emerged the earliest flag leaf in July and August, significantly decreased DOY. For DOY from ST to FS2, M. sacchriflorus germplasms ranged from 140 to 190 days, and 110 to 170 days for 2013 and 2014. The highest frequency showed to 160 days for 2013, and 150 days for 2014. In M. sinensis germplasms, the highest frequency showed to 180 days for 2013, and 170 days for 2014. In the results of correlation between the day of years from ST to FS2 for 2013 and 2014, M. sacchriflorus and M. sinensis showed high coefficient of correlation (0.70 and 0.89). It can be supposed that flowering characteristics of Miscanthus are largely affected by the unique phenotypic characteristic of native habitat than environmental factors of the current planted site. This study for flowering traits of Miscanthus may provides an important information in order to expedite the introduction as breeding materials for creation of new hybrid.