간행물

한국작물학회지 KCI 등재 Korean Journal of Crop Science

권호리스트/논문검색
이 간행물 논문 검색

권호

제47권 5호 (2002년 12월) 10

1.
2002.12 서비스 종료(열람 제한)
To evaluate the efficiency of anther floating culture according to the maturing group, the varietal difference and classification of fifty varieties was conducted in N6 liquid medium containing 1mg l-1 NAA, 0.25 mg l-1 kinetin. The efficiency of callus induction was widely ranged from 0 to 113.4%, but the mean callus induction was not significantly different among maturing groups. The efficiency of anther floating culture showed the highest variation in early-maturing group among three maturing groups. The varieties with the best callus induction were Sambaegbyeo and Jinbuolbyeo, while the recalcitrant variety was Obongbyeo in early-maturing group. The efficiency of plant regeneration showed the highest trends in late-maturing group among three maturing groups. The fifty varieties were classified into three groups (distance=0.78) by cluster analysis based on the callus formation and plant regeneration. Group including only two varieties, Shinunbongbyeo and Sambaegbyeo had the excellent androgenic efficiency, and the medium efficiency of Group was included thirty-six varieties. Whereas twelve varieties, including three Tongil varieties were fell into the bad efficiency of Group. Especially, Tongil varieties containing Japonica rice, Obongbyeo were the recalcitrant genotypes for the anther floating culture.
2.
2002.12 서비스 종료(열람 제한)
In order to examine the mechanistic basis for differential sensitivities to chilling and subsequent recovery between two rice (Oryza sativa L.) cutivars, a chilling-tolerant japonica type (Ilpumbyeo) and a chilling-susceptible indica type (Taebaekbyeo), changes of physiological responses and antioxidant enzymes were investigated. Both cultivars at 3 leaf stage were exposed at a low temperature of 5~circC for 3 days and subsequently recovered in a growth chamber at a 25~circC for 5 days with 250 mmol m-2 s-1 . Physiological parameters such as leaf fresh weight, relative water content, cellular leakage, lipid peroxidation, and chlorophyll a fluorescence showed that the chilling tolerant cultivar had a high tolerance during chilling. However, the chilling-susceptible cultivar revealed severe chilling damages. The chilling-tolerant cultivar was also faster in recovery than the chilling-susceptible cultivar in all parameters examined. We analyzed the activity and isozyme profiles of four antioxidant enzymes which are: superoxide dismutase (SOD), caltalase (CAT), ascorbate peroxidase (APX), and glutation reductase (GR). We observed that chilling-tolerance was due to a result of the induced or higher antioxidant enzyme system, CAT and APX in leaves and SOD, CAT, APX, and GR in roots. Especially, we observed the most significant differences between the chilling-tolerant cultivar and -susceptible cultivar in CAT and APX activity. Also in isozyme profiles, CAT and APX band intensity in the chilling-tolerant cultivar was distinctively higher than in the chilling-susceptible cultivars during chilling and recovery. Thus, the cold stability of CAT and APX are expected to contribute to a tolerance mechanism of chilling in rice plants. In addition, the antioxidative enzymes activity in roots may be more important than in that of leaves to protect chilling damage on rice plants.
3.
2002.12 서비스 종료(열람 제한)
Mature embryos of five oat genotypes were cultured to develop an efficient method of callus induction and plant regeneration. Murashige and Skoog(MS) and N6 media supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin were used for callus induction. Percentage of callus induction showed significant among the combinations of plant growth regulators. Callus induction showed high efficiency in medium containing 3 mg/~ell of 2,4-D. The high frequency of callus induction was obtained in Gwiri37. For plant regeneration, calli induced from mature embryos were transferred onto MS and N6 media supplemented with combinations of 6-benzyladenine (BA) and naphthaleneacetic acid (NAA) for 5 weeks. Percentage of plant regeneration showed high in MS medium containing 0.2 mg/~ell of NAA and 1 mg/~ell of BA. The callus initiation medium affected the subsequent plant regeneration. Treatment with 3 mg/~ell of 2,4-D, and 3 mg/~ell of 2,4-D and 3 mg/~ell of kinetin in callus induction media showed high frequency for plant regeneration. Plant regeneration frequency among the genotypes showed significant. Especially, Gwiri37 showed high regeneration frequency. Regenerated shoots were treated with 200, 350 and 500 mg/~ell of indole-3-butyric acid (IBA) transferred onto half-strength MS medium without plant growth regulators. Treatment of shoots with IBA induced root formation rapidly
4.
2002.12 서비스 종료(열람 제한)
The seedlings of yacon (Polymnia sonchifolia Poeppig & Endlicher), which were cuttings, plug seedling (PS), crown bud before sprouting (CBBS), crown bud after sprouting (CBAS), and divided seedling after budding (DSAB) were planted at 70~times 50 cm planting distance on ridge; that was interow spacingintrarow spacing, and about 28,500~pm 71 plants/ha. CBBS didn't need work and equipment to raise seedlings. PS and DSAB grew taller to 140.5 and 143.3cm, respectively, than others at 150 days after planting. In the changes of plant height, PS and DSAB showed taller than others during growth period, cuttings, CBBS, and CBAS grew rapidly in middle growth stage. Excepting main stem and petiole length, other characters were significant for seedling. Fresh weights were different among seedlings. Even though the yield of plants grown from CBAS and CBBS were lower with 34.7 and 36.4 ton/ha, respectively, than 3.6 ton/ha of DSAB; its yield index were over 95%, hence, those of plants grown from cutting and PS were lower with 73 and 87%, respectively. The ratio of tuberous roots over 200g to total tuberous roots per plant was the highest from DSAB. Most of tuberous roots were under 200g per tuberous root from cuttings. CBBS, CBAS, and DSAB are suitable to use seedlings for high yield of yacono. Yacon plant by DSAB much produced tuberous root of over 200g.
5.
2002.12 서비스 종료(열람 제한)
In order to determine the optimum harvest time for the seed production of inbreds and hybrids in silage corn, the ears of sib-pollinated 'KS5', 'KS7rhm', and 'Ga209' and cross-pollinated 'KS5' ~times 'KS6' (Suwon19), 'KS7 rhm' ~times 'KSl17' (Suwonok), and 'Ga209' ~times 'DB544'(Kwanganok) were harvested at the one-week intervals from 4 to 10 weeks after silking. The optimum harvest time for the seed production for 'KS5', 'KS5' ~times 'KS6', 'KS7 rhm', and 'KS7rhm' ~times 'KS117' was 7 weeks after silking considering both emergence rate and plumule growth in cold test. Although earlier harvested seeds showed similar germination rate as the seeds harvested at the optimum time at 25~circC , their emergence rate were lower in cold test. Seed weight and α -amylase activity of earlier harvested seeds were lower compared to those of seeds harvested at the optimum time, while leakage of total sugars and electrolytes were higher. However, the later harvested seeds showed lower germination rates at 25~circC and emergence rates in cold test probably due to the lower α -amylase activity although they showed increased seed weight and reduced leakage of total sugars and electrolytes. In contrast, the emergence rate of 'Ga209' and 'Ga209' ~times 'DB544' in cold test increased up to 10 weeks after silking probably due to the increased seed weight and α -amylase activity and reduced sugar and electrolyte leakages during the germination. The cross-pollinated F1 hybrid seeds showed higher germination and emergence rates at 25~circC and in cold test, and higher plumule growth and α -amylase activity compared to those of sib-pollinated inbreds.
6.
2002.12 서비스 종료(열람 제한)
The strip intercropping system has used due to many advantages. Many researches supported these crop systems are usually equal to or better than monoculture crop systems in both total production and profit. There was no research to examine the important ecological factors in the competition between crops. A strip intercropping system composed of adjacent narrow strips of corn, soybean, and oat/legumes has been investigated in Iowa, USA. This study conducted to investigate why and how the differences in soybean yield are produced and affected by light, one of the microclimate, of the strip intercropping system. In height, the two rows of soybean closest to corn were taller than the two rows near the then-empty oat strip. The height of each crop decreased as the amount of light received increased. Weight of plant parts was lowest in row 1, nearest corn, and highest in row 4, next to the vacant oat strip. Daily photon flux density(PFD) increased with increasing distance from corn, with the highest value occurring on the edge next to the empty oat strip. Analyses of the relationship between light and biomass of soybean showed that all biomass measurements had a positive relationship to total PFD per day except height.
7.
2002.12 서비스 종료(열람 제한)
To evaluate growth habits, fresh pod yield potential, and possibility of early and late seeding, seeding dates were extended from March 21 to June 20 by PE mulching and non-mulching. Soil temperature, under 5cm from surface, above 15~circC at 10 a.m. in early seeding reached about March 25 in mulching and April 5 to April 12 in non-mulching. Days to emergence and first flowering were accelerated owing to increasing temperature, as seeding was delayed. Days to emergence according to seeding dates reduced 21 to 8 day in mulching and 33 to 10 day in non-mulching. Days to flowering were ranged from 51 to 26 day in mulching and from 69 to 32 day in non-mulching and differences between mulching and non-mulching on each seeding date had 18 to 4 days. Early seedings till April 21 had 160-170 flowers per plant for 8 weeks, while late seedings from May 21 increased more speedily with 200 flower for 6 weeks. Harvesting of fresh peanut, at 80 days after first flowering, was possible from Aug. 1 to Oct. 7 (133-108 days to harvest) by mulching and from Aug. 19 to Oct. 12 (151 to 114 days) by non-mulching. Yields between mulching and non-mulching in early seeding until April 21 had more difference, but in late seeding after May 21 was higher and showed insignificance. Pod setting periods by early and late seeding were about 3 weeks equally. In late seeding pod setting were almost concentrated for front 15 days. In spite of difference of fresh pod weight between two seeding times, the distributions of average of seed weight showed nearly same tendency.
8.
2002.12 서비스 종료(열람 제한)
Allelic diversity of 44 microsatellite marker loci originated from the coding regions of specific genes or the non-coding regions of barley genome was analyzed for 19 barley genotypes. Multi-allelic variation was observed at the most of marker loci except for HVM13, HVM15, HVM22, and HVM64. The number of different alleles ranged from 2 to 12 with a mean of 4.0 alleles per micro-satellite. Twenty-one alleles derived from 10 marker loci are specific for certain genotypes. The level of polymorphism (Polymorphic Information Content, PIC) based on the band pattern frequencies among genotypes was relatively high at the several loci such as HVM3, HVM5, HVM14, HVM36, HVM62 and HVM67. In the cluster analysis using genetic similarity matrix calculated from microsatellite-derived DNA profiles, two major groups were classified and the spike-row type was a major factor for clustering. Correlation between genetic similarity matrices based on microsatellite markers and pedigree data was highly significant (r=0.57** ), but these two parameters were moderately associated each other. On the other hand, RAPD-based genetic similarity matrix was more highly associated with microsatellite-based genetic similarity (r=0.63** ) than coefficient of parentage.
9.
2002.12 서비스 종료(열람 제한)
No-tillage (NT) practice for corn production has advantages of reduction of soil erosion and energy conservation. Research on effects of NT for sweet corn or super sweet corn is very limited. Hybrids of sugary (su) and shrunken-2 (sh2) were tested under NT and conventional tillage (CT) practices to investigate plant characters, ear characters, fresh yield, and grain yield. Sugary hybrids were Golden Cross Bantam 70 (GCB70), Sprint, Geumdanok, and Danok3. Shrunken-2 hybrids were BSS9472, Cambella90, GSS9299, Jubilee, KS-Y-65, and Chodangok1. Emergence rates under NT were lower than those under CT for su, while there was no difference between tillage systems for sh2. There were no differences between CT and NT for days to tasseling and silking, plant height, and ear height for both su and sh2. Ear characters such as ear length, number of kernel rows, number of kernels per row, and t100-kernel weight under NT were not significantly different from those under CT. There were no differences between two tillage practice for fresh and grain yield, rather they showed trend of increases under NT practices. Results from this trial indicate that NT practice for both su and sh2 cultivation may be possible to recommend to farmers.
10.
2002.12 서비스 종료(열람 제한)
Spikelet number per unit area(SPN) is a major determinant of rice yield. Nitrogen nutrition status and biomass during reproductive stage determine the SPN. To formulate a model for estimating SPN, the 93 field experiment data collected from widely different regions with different japonica varieties in Korea and Japan were analyzed for the upper boundary lines of SPN responses to nitrogen nutrition index(NNI), shoot dry weight and shoot nitrogen content at panicle initiation and heading stage. The boundary lines of SPN showed asymptotic responses to all the above parameters(X) and were well fitted to the exponential function of f(X)=alphacdot1- etacdotexp(gamma;cdot;X) . Excluding the constant, from the boundary line equation, the values of the equation range from 0 to 1 and represent the indices of parameters expressing the degree of influence on SPN. In addition to those indices, the index of shoot dry weight increase during reproductive stage was calculated by directly dividing the shoot dry weight increase by the maximum value (800 extrmg/m-2 ) of dry weight increase as it showed linear relationship with SPN. Four indices selected by forward stepwise regression at the stay level of 0.05 were those for NNI (INNIP ) at panicle initiation, NNI(INNIh ) and shoot dry weight(IDWh ) at heading stage, and dry weight increase(IDW ) between those two stages. The following model was obtained: SPN=48683ㆍ IDWH 0.482 ㆍ INNIp 0.387 ㆍ INNIH 0.318 ㆍ IDW 0.35 ). This model accounted for about 89% of the variation of spikelet number. In conclusion this model could be used for estimating the spikelet number of japonica rice with some confidence in widely different regions and thus, integrated into a rice growth model as a component model for spikelet number estimation.n.n.