Optically Adaptive System for Imaging Spectroscopy (OASIS) 3D data of the Canada-France-Hawaii Telescope in the central 10''.48''.3 (2.92.3 kpc2) region of the Seyfert 2 galaxy NGC 1358 were analyzed. Emission line maps for H at 6563 Å and H at 4861 Å were obtained from the OASIS spectra in the 4800-5500 and 6220-6990 Å wavelength regions. Density distribution, as indicated by the H/H flux ratio, is 105.5 cm3 at the center and 104.4-106.0 cm3 surrounding the center. An elliptical region with a density of 105.5-105.8 cm-3 (perpendicular to the bar's position angle, PA) was discovered at symmetrical locations approximately 1.2-2.0 arcsec and 1.1-1.9 arcsec south east (SE) and north west (NW), respectively, of the center along the bar (PA=130o) axis. A lower-density region (appearing as a void) also existed between the center and this symmetrical structure. The high F(H)/F(H) flux ratio values and the distribution of line widths suggest a region with high-density neutral hydrogen gas. The H flux image and linewidth, and F(H)/F(H) flux ratio image maps suggest presence of a substructure associated with a supermassive black hole at the galactic center, as well as independent structures with relatively strong fluxes in the SE and NW regions. The SE structure is delineated as one substantial substructure, whereas the NW substructure appears broken, or is potentially two separate structures, due to dust shielding. The regions have an independent boundary layer with a density exceeding 106.0cm3 toward the center, likely resulting from collision of the structure flowing along the bar with the Inner Lindblad resonance zone.

Toxic gas emissions are a critical global health concern, responsible for numerous deaths each year. These hazardous gases can cause severe physiological reactions and even death upon exposure. To address this issue, we propose a graphene-Kaptonbased flexible biosensor for non-invasive toxic gas detection. The sensor is designed to accurately detect and identify several harmful gases, including carbon monoxide (CO), fluorine azide ( FN3), hydrogen iodide (HI), nitrogen ( N2), methane ( CH4), nitrous oxide ( N2O), and ozone ( O3). Utilizing the Computer Simulation Technology (CST) Studio Suite 2024, we simulate the detection process, focusing on advanced techniques and miniature flexible structures. The sensor’s active element is a graphene patch embedded within a polyimide (Kapton) film, which allows for precise determination of the RF planar resonant structure’s frequency response. The graphene–Kapton biosensor is shown to have remarkable detection performance, as demonstrated by the results of the simulation, with a diffusivity of 9.09e−08[m2∕S] , an accuracy of 6.62e−13 , and a power loss of 1.5mW . These findings highlight the sensor’s potential as an effective tool for detecting and identifying toxic gases with high precision and efficiency.

Fetal Bovine Serum (FBS) plays a crucial role in animal cell culture; however, the increasing number of bovine fetuses used and sacrificed solely for FBS collection has raised ethical concerns globally. The welfare of fetuses during FBS blood collection has become a key focus of debate among animal welfare and ethics organizations worldwide. Previous studies indicate that heat-inactivated coelomic fluid (HI-CF) from the earthworm Perionyx excavatus may serve as a viable FBS alternative in adherent cell cultures. This study evaluates the potential of HI-CF as an FBS substitute during the in vitro maturation (IVM) stage of bovine embryo culture, with a focus on improving developmental rate through antioxidation effects. In this study, 2% HI-CF was incorporated into IVM media, assessing its impact on cell growth, differentiation, and the expression of genes related to antioxidation. The group of 2% of HI-CF exhibited a trend toward increased cleavage and blastocyst development rates compared to the control group. Although antioxidant genes such as NRF2 and GSR showed no statistically significant differences between the control and treatment groups, a trend toward increased expression was observed. Conversely, GPX1 displayed a trend of decreased expression. Notably, IGF1 and NQO1 were significant upregulated (p < 0.05) in the 2% HI-CF group. Additionally, oocytes stained with H2DCFDA showed a significantly reduced ROS levels (p < 0.05) in the 2% HI-CF group compared with controls. These findings suggest that HI-CF's antioxidative effects support enhanced cell growth and blastocyst development rate, surpassing those observed with FBS. Consequently, HI-CF shows promise as an effective alternative to FBS in vitro maturation of bovine oocytes.