Nutrient acquisition by insect herbivores affect all aspect of the the lifespan of individauls. For seed-sucking insect herbivores, they face challenges with nutrient acquisition due to requirement for extra-oral digestion of seed contents into a readily-ingestible state. In this study, we demonstrated environmentally-transmitted Caballeronia insecticola allow seed-sucking R. pedestris to overcome challenges with extra-oral digestion. Through the evaluation, first, we found symbiotic insects exhibited enhanced feeding efficiency by consuming significantly larger amount of food per feeding attempt compared to apo-symbiotic insects (P<0.05). Then, we observed feeding behavior modification in the symbiotic insects from the behavior tracking evaluation. Symbiotic insects displayed dichotomic behavior which can be generally divided into early focused feeding and later subdued resting periods. By contrast, apo-symbiotic insects displayed unordered behavior by frequent switches between feeding and walking behavior.

One of the overlooked points in mosquito blood feeding research is a final step before blood feeding. We provide the anatomical and chemosensory evidence that a piercing structure of the mouthpart of the mosquitoes is an essential apparatus for the penultimate stage in blood feeding in mosquitoes. Indeed, the stylet possesses a number of sensory hairs located at the tip of the stylet. These hairs house olfactory receptor neurons that express two conventional olfactory receptors of Aedes aegypti (AaOrs), AaOr8 and AaOr49, together with the odorant co-receptor (AaOrco). In vivo calcium imaging using transfected cell lines demonstrated that AaOr8 and AaOr49 were activated by volatile compounds present in blood. Taken together, we identified olfactory receptor neurons in the stylet involved in mosquito blood feeding behaviors, which in turn indicates that olfactory perception in the stylet is necessary and sufficient for mosquitoes to find host blood in order to rapidly acquire blood meals from a host animal.

담배가루이 등 방제용 천적으로 유망한 담배장님노린재(Nesidiocoris tenuis)의 줄알락명나방(Ephestia kuehniella) 알 섭취 전 먹은 식물성 먹이에 따라 줄알락명나방알 섭취량이 달라지는지를 알아보기 위해 포트에 재식된 참깨, 파프리카, 솜에 적신 물을 각각 하루 동안 섭식하게 한 담배장님노린재에게 냉동한 줄알락명나방의 알을 공급하고 하루 동안 섭취한 알의 개수를 비교하였다. 파프리카를 먼저 섭식한 담배장님노린재가 가장 많은 55.7±30.19개의 줄알락명나방 알을 섭취한 것으로 나타났으며, 참깨를 먼저 섭식한 경우에는 줄알락명나방 알을 24.2±34.70개 섭취하였고, 물을 먼저 섭식한 경우에는 줄알락명나방 알을 49.2±30.58개 섭취하였다. 파프리카와 물을 먼저 섭식한 경우 통계적인 유의차는 보이지 않았다. 그러나 참깨를 먼저 섭식한 경우 파프리카 또는 물을 먼저 섭식한 경우에 비해 더 적은 수의 줄알락명나방의 알을 섭취한 것으로 나타났다.

This study was carried out to know whether the host plant feeding have an influence to subsequent prey consumption of a zoophytophagous mirid, Nesidiocoris tenuis, with biological control potential. Piece of leave of Paprika, Sesame, and cotton ball soaked with water were respectively provided to the test insects, adult mirid starved about a day, and frozen eggs of Ephestia kuehniella, were presented to them for a day and counted the number of the eggs consumed by them. The mirid fed by Sesame leave took significantly less prey than both the Paprika and the water fed one.

This study was performed to compare the host preference, survivorship and feeding behavior using EPG against ggot-mae-mi, Lycorma delicatula against seven plants such as Ailanthus altissima, Vitis vinifera, Malus pumila, Pyrus calleryana, Hibiscus syriacus, and Pinus densiflora. In host preference. L. delicatula was most preferred the Ailanthus altissima, Vitis vinifera and was not preferred the other plants. Survival rate of 3rd Nymph was recorded from Ailanthus altissima, Vitis vinifera as 15.0, and 15.4 days, respectively, it showed longest period. However, Malus pumila, Pyrus calleryana, Hibiscus syriacus, were survived within 6 days and Pinus densiflora was within 5 days. Moreover, L. delicatula was survived within 2 days to the three kinds of fruits. Ecdysis rate from 3rd to 4th nymph also high from Ailanthus altissima, Vitis vinifera as 63.3, 63.0 % and the order was followed as Malus pumila(17.7%) > Pyrus calleryana(9.3%) > Hibiscus syriacus(7.8%) > Pinus densiflora(5.9%). Especially, ecdysis rate was recorded 0% to the three kinds of fruits. Feeding behavior was analyzed using EPG and compared the differences their waveform from seven kinds of plants and three kinds of fruits. Non-probing time was short in host plants, reversely, Phloem-feeding time was recorded longer in Ailanthus altissima, Vitis vinifera as 45.7 and 13.7 min, respectively. And other plants and fruits were not showed feeding behavior.

아메리카잎굴파리의 내부기생봉인 Neochrysocharis formosa의 산란과 기주체액섭취 선호성을 조사하였다. N. formosa는 기주유충의 몸 뒤쪽에 대부분 산란하였다. 온도 조건에 따른 일산란수와 일기주체액섭취수를 조사한 결과 온도가 높을수록 증가하는 경향이었으며 15, 20, 25, 3에서의 산란수와 체액섭취수의 비율은 각각 0.5, 0.9, 1.0, 1.0이었다. 항온조건하에서 N. formosa의 총산란수와 기주체액섭취수는 각각 176개 188.7개이었으며 산란수와 기주체액섭취수 비율은 0.9이었고 암컷 수명은 11.8일이었다. 산란과 기주체액섭취는 주로 아메리카잎굴파리 3령유층을 선호하였다.