In the present study, we investigated the protective effect of various grain methanolic extracts against UVB-induced photo-aging in human skin fibroblasts. Various grain methanolic extracts were evaluated for their antioxidant compounds and activities. 2,2-Ddiphenyl-1-picryhydrazyl radical (DPPH) and ABTS 2,2-azino-bris-(3-ethylbenzoth iazoline-6-sulphonic acid) radical cation scavenging activities have been used to measure the relative antioxidant activities of extracts from grains. The content of total polyphenolics in the extracts were evaluated using spectrophotometric methods. Human skin fibroblast (Hs68) cells were pretreated with various grain methanolic extracts (25 μg/mL). Skin toxicity was simulated by exposing the cells to UVB (30 mJ/cm2) irradiation. In response to the UVB-irradiation, an increased amount of matrix metalloproteinases (MMPs) release was observed, whereas pretreatment of various grain methanolic extracts significantly inhibited the production of MMP-1 in Hs68 cells. We also found that pretreatment of the extracts significantly decreased UVB-induced reactive oxygen species and significantly increased total collagen content in Hs68 cells. These results provide that grains could be regarded as a potential ingredient in natural cosmetics, used for UVB protection.

본 연구에서는 장미꽃 methanol 추출물의 항염증 활성을 조사하기 위하여 LPS에 의해 염증이 유도된 RAW 264.7 대 식세포에서 염증억제 효과를 알아보았다. 염증 억제의 지표 로서는 세포가 방출하는 NO 생성량과 iNOS 및 NF-κB 발현정도를 측정하였다. 실험 결과, RAW 264.7 대식세포에 대한 품종별 장미꽃 methanol 추출물(500 μg/mL)이 NO의 함량을 감소시키는 경향을 나타내었다. NO의 생성에 영향을 미치는 iNOS 단백질의 발현량을 측정한 결과, LPS 처리에 의해 활성 화된 iNOS 단백질의 발현이 장미꽃 methanol 추출물 처리 시 유 의적으로 수준으로 억제하는 경향을 보였다. Luciferase activity 를 실행한 결과, LPS로 자극한 세포와 비교하였을 때 염증과 관련된 NF-κB promoter activity가 장미꽃 methanol 추출물 처 리시 현저히 감소하는 경향을 나타내었고, 세포질의 IκBα의 인산화를 저해함으로써 전사요소인 NF-κB p65, p50을 핵 속 으로 유리시키는 과정을 억제하였다. 이 결과로 장미꽃 methanol 추출물이 전사단계에서 저해활성을 나타낸다는 것을 확인하 였다. 본 연구결과, 장미꽃 methanol 추출물은 항염증 효과를 나타냄에 따라 만성 질환 예방을 위한 기능성 식품의 원료로 활용될 수 있을 것으로 여겨진다.

To evaluate the antioxidant and potential of Humulus japonicus, total polyphenol and flavonoid content, radical scavenging activities, and antitumor activities were measured. The total polyphenol and flavonoid contents of the methanol extracts from Humulus japonicus were 30.13±1.13 and 13.61±0.49 ㎎ gallic acid equivalent/g extract, respectively. DPPH radical and hydroxyl radical scavenging activities of methanol extracts of Humulus japonicus were 60% and 35%, respectively. The Humulus japonicus higher activities of anticancer activities on liver cancer cell lines compared to other cancer cell lines.

MTT(3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium) assay were used to analyze anticancer activities from herbal plants. This study was conducted to inhibit the human lung cancer(A549) cell line in vitro. The 1C50 value of cytotoxicity showed range of 127.83-2018 .1 9μg/m~. Especially Coriander( 127.83μg/mR} and Catnip(140.68μg/me.) showed more stronger activity than other plants, respectively.

국내 식물자원 및 약용식물들의 식물 생장촉진 효과를 조사하기 위하여 64종 65점의 추출물 시료를 조제하여 벼(Oryza sativa) 유묘 생장에 미치는 영향을 평가하였다. 그 결과, 11종 12점의 쑥(Artemisia princeps) 경엽, 엉겅퀴(Cirsium japonicum var. maackii) 전초, 진달래(Rhododendron mucronulatum) 가지와 잎, 갈대(Phragmites communis) 줄기, 율무(Coix lacrymajobi var. mayuen) 전초, 싸리(Lespedeza bicolor) 가지와 잎, 산수국(Hydrangea serrata f. acuminata) 전초, 속단(Phlomis umbrosa) 전초, 감초(Glycyrrhiza uralensis) 근경, 감초(G. uralensis) 경엽, 당귀(Angelica gigas) 뿌리 및 천궁(Cnidium officinale) 근경 추출물은 벼 유묘 생장을 촉진시키는 것으로 나타났다. 본 결과에 따라 상기 식물 종의 부위들은 식물 생장 촉진용 농가 자가제조 액비 개발을 위한 소재로써 활용가능성이 있는 것으로 판명되었다.

A variety of herbs and plants have been traditionally used in oriental folk medicine for the treatment of inflammatory diseases. In our attempt to search for anti-inflammatory agents from natural products, we investigated 64 methanol extracts from 42 medicinal plants belonging to 10 families which were evaluated for inhibitory activities of NO production in lipopolysaccharide (LPS)-stimulated macrophage RAW 264.7 cells. Among them, 16 extracts exhibited inhibitory activities of NO production (IC50 values ranging from 59.6 to 94.7 μg/ml). Only the extract from aerial parts of Hosta lancifolia (H. lancifolia) did not exert cytotoxic effects at the concentrations tested. The extract from H. lancifolia decreased the mRNA and protein levels of inducible nitric oxide synthase (iNOS) and pro-inflammatory cytokines in activated macrophage RAW 264.7 cells in dose-dependent manner. The results suggest that the extract may contain bioactive compounds that suppress expression of pro-inflammatory cytokines, which may prove beneficial with regard to the development of natural agents for prevention and treatment of inflammatory diseases.

매실박 메탄을 추출물의 항균력을 조사한 결과, 이상의 농도에서 뚜렷한 항균활성을 보였고, 광역의 변패성 미생물들에 대하여 항균활성을 나타내었다. 또한, 매실박 메탄올추출물의 항균물질은 높은 온도에서 30분 동안 안정하였으며, 넓은 범위의 pH(3-11)에서 안정성을 나타냈다. 따라서 매실박 메탄올 추출물의 항균물질은 항균활성이 높고, 항균s spectrum이 광범위할 뿐 아니라, 높은 온도 및 넓은 범위의 pH에 안정하여 이상적인 천연 항균제로