A diverse of recommendation has been made for the structure and management of dairy cows, despite demanding research, the relationship between lactation number and various factors is yet to be established. The present study was aimed to investigate the covariance among lactation number, growth performance, calving interval, and milk production was considered to increase an efficiency of selection schemes and to manage more efficiently Holstein dairy cows that have been raised on small-scale family farms in Republic of Korea. For that purpose, the data were observed from 850 Holstein dairy cows, which a total of 3929 milking, since April 2016 - January 2017. We measured the body weight, height, age, calving interval, and milk production of the each dairy cow. Also, information about the date of lactation, calving interval, and milk production was recorded using an automatic milking system(AMS) with identification numbers. Milk production was calculated per udder quarter in the AMS. Our study results showed the increased average body weight(p>0.05) in 1, 2, 3, and 4th lactating dairy cows and afterwards, we noticed the tendency on the average body weight(p<0.05) per lactation progressed. There was no significant difference noticed on height measurement of dairy cows. From the processing data of 850 Holstein dairy cows, the lactation number 1 and 7 had a greater calving interval with significantly lowered milk production, and the lactation number 2, 3, 4, 5, and 6 had significantly lowered the calving interval(p<0.05) with a greater milk production. From our study results, we evidenced that there is a significant relationship between the lactation number, growth performance, calving interval, and milk yield, and the maximum production of milk occurring in the 3rd and 4th lactation dairy cows. The achieved results from this study can be used by the small-scale farmers to encourage the structure and management of growth performance, calving interval, and milk yield in Holstein dairy cows in Korea.

본 조사연구는 충남 예산지역에 위치하고 있는 젖소 사 육농가 중 10개 전업 검정농가를 대상으로 2015년 1월부터 2015년 12월까지 농협중앙회 젖소개량사업소의 검정성적 을 통하여 유량 및 유조성분을 조사·분석하여 젖소농가의 경영지표 설정과 농가의 경영기술 개선을 위한 기초적 자 료를 얻고자 실시한 결과를 요약하면 다음과 같다. 평균 산유량은 33.62kg로 나타났다. 산차별로는 유의한 차이 (p<0.05)를 보였으며 1산차는 29.60kg로 가장 낮은 산유량 을 나타났으나, 2산차 이상부터 높은 산유량을 보였다. 산 유량별로도 유의한 차이를 보였으며(p<0.05), 계절별 평균 산유량은 봄에 34.51kg으로 다른 계절보다 높은 유량을 보 였다(p<0.05). 산차별의 유지율은 유의성 없이 1산에서 6산 차까지 비슷한 결과를 보였다. 산유량별 간에 유지율은 유 의한 차이(p<0.05)가 있었고, 계절별 간에는 유의한 차이 (p<0.05)를 보였는데, 겨울에 유지율 4.15%로 가장 높은 성 적을 보였으나, 여름에 3.61%로 가장 낮은 함량을 보였다. 산차별 간의 단백율은 유의한 차이 없이 평균치와 비슷한 수준을 보였다. 산유량별 간의 유단백율은 유의한 차이 (p<0.05)로 1일 30kg미만 군에서는 3.51% 높은 유단백율을 보였고, 40kg이상 군은 3.14%로 가장 낮은 함량으로 나타났다. 계절별 간의 유단백율은 가을과 겨울은 각각 3.40% 와 3.41%으로 높았으나, 봄과 여름은 각각 3.28%와 3.27% 로 낮은 수준를 보였다(p<0.05). 총고형분은 산차별 간에 1 산차가 8.92%로 가장 높은 함량를 보였으나(p<0.05), 산차 가 커질수록 감소현상을 보였다. 산유량별에 따른 총고형 분율은 1일 30 kg미만 군에서 8.92%로 높았으나, 40kg이상 군은 8.62%로 낮은 총고형분율을 나타났다(p<0.05). 계절 별 간의 총고형분율은 가을과 겨울에 각각 8.87%와 8.84% 으로 높은 함량이 었으나, 봄과 여름에는 각각 8.77%와 8.75%로 낮았다(p<0.05). 산차별간의 체세포수는 1산차가 100.75천개/ml로 가장 낮은 수를 나타냈으나, 산차가 커질 수록 체세포수는 증가현상을 보였다(p<0.05). 산유량별로 는 1일 30kg미만 군은 157.00천개/ml의 높은 체세포수로 유의한 차이를 보였으나(p<0.05), 40kg이상 군의 123.64천 개/ml로 낮은 수로 나타났다. 산유량은 유지율, 유단백율 및 총고형분율과 각각 -0.378, -0.534 및 –0.370으로 음(-)의 상관계수를 나타냈다. 유지율은 유단백율 및 총고형분율과 의 상관계수는 각각 0.600과 0.493으로 매우 높은 양(+)의 관계를 보였다. 유단백율과 총고형분율 간에는 0.833으로 양(+)의 매우 높은 상관관계를 보였다. 산유량과 유지율의 단순회귀모형은 y=-0.0297x+4.907(R2=0.1426), 유지율과 유 단백율은 y=0.2824x+2.2482(R2=0.3606), 유단백율과 총고형 분율은 y=0.8564x+5.9404(R2=0.6942)를 나타냈다. 따라서 젖소의 산유량과 유성분은 계절적 요인, 그리고 체세포수 는 산차와 산유능력에 영향을 받는 것으로 나타나 적절한 젖소의 산유량과 유질개선에 각별한 관리가 요구된다.

This study aimed to investigate the effects of Dandelion (Taraxzcum coreanum) supplementation on milk yield, milk composition and blood characteristics in lactating dairy cows. Eight lactating dairy cows were divided into two groups (control: TMR supplementation, treatment: TMR with Dandelion supplementation). The milk yield, milk fat, lactose, solids not fat (SNF) and somatic cells counts (SCC) were not significantly different between the control group and the treatment group, whereas milk protein, milk urea nitrogen (MUN) and free fat acid (FFA) were significantly higher in the treatment group compared to the control (p<0.05). The blood components of the treatment group were compared with those of the control group and only aspartate aminotransferase (AST) appeared significantly high (p<0.05). The other blood components were not significantly different in the two groups. Blood corpuscle components in the groups were not significantly different. Especially, all blood corpuscle components in the treatment group were within the normal range. However, the white blood cells (WBC), lymphocytes (LYM) and hematocrits (HCT) in the control group exceeded the normal range. Based on the above results, the addition of Dandelion to feed increased milk protein, MUN and FFA, but did not significantly affect the composition of the blood and corpuscle in Holstein milking cows.

반추위 pH 조정제로서 일반적으로 중조가 많이 사용되고 있으나 사료에 중조의 첨가량을 증가시키는 경우에 기호성을 저하시키는 경향이 있다. 따라서 본 연구에서는 사료의 기호성에 영향을 미치지 않으면서 반추위 완충효과를 나타내도록 개발된 액상형 완충제의 젖소사료 첨가제로서의 이용효과를 구명하기 위하여 수행되었다. 시험축으로 착유 중기 젖소 27두를 공시하였으며, 처리는 대조구와 중조 첨가구, 그리고 액상형 완충제 첨가구로 하여 처리별 9두씩 배치하였다. 대조구는 착유우용 자가배합 TMR 사료를 급여하였으며, 중조 첨가구는 대조 사료에 중조를 두당 1일 150g 추가하여 급여하였고, 액상형 완충제 첨가구는 같은 방식으로 액상형 완충제를 두당 15㎖ 추가하여 사양시험을 수행하였다. 시험결과 산유량은 대조구, 중조 및 액상형 완충제 첨가구의 경우에 각각 23.82, 23.91, 24.91kg으로 대조구에 비하여 액상형 완충제 처리구에서 다소 증가하는 경향이었으나 처리 간에 뚜렷한 유의 있는 결과를 보여주지는 않았다. 처리에 따른 유성분 변화에서는 유단백질 및 무지고형분에서 액상형 완충제를 첨가한 경우에 대조구에 비해 유의 있는 증가(p < 0.05)를 보여주었고, 유지율에서도 대조구에 비하여 처리구가 다소 증가하는 경향을 보였다. 혈액성분 중 포도당과 콜레스테롤은 대조구에 비해 액상형 완충제의 첨가구에서 다소 증가하였으나, 처리 간에 유의 있는 차이를 나타내지는 않았다. 따라서 개발된 액상형 완충제의 추가급여 시 생산성 증대, 특히 유성분 증가 효과가 있어 반추위 pH 조정을 위하여 일반적으로 이용되고 있는 중조를 대체하여 반추동물의 사료첨가제로 이용이 가능할 것으로 평가되었다.

This study was conducted for SNPs in the 5'-regions of estrogen receptor-α(ESR-α), and association with calving interval (CI), service per conception (SPC) and 305 days milk yield in Hanwoo and Holstein dairy cattle. The genet-ic improvement was incurred low reproduction performance. The objective of this study was to investigate connec-tions between single nucleotide polymorphisms (SNP) of Estrogen receptor-α (ESR-α) with reproduction performance (calving interval, service per conception, and 305 d milk yield) in Hanwoo and Holstein dairy cattle. Hanwoo and Holstein blood samples were collected from 183 and 124 dam of breeding farms and DNA was extracted. Primer design was based on NCBI GenBank (Accession No. AY340579). The PCR-RFLP method with Bgl I was used to ge-notype the cattle. The result showed two variants of the ESR-α gene. The Bgl I cut the 492 bp amplification pro- duct into 322 bp and 170 bp fragments for allele G, while allele A remained uncut, resulting in two restriction frag-ments for homozygote G/G and three fragments for heterozygote A/G. We found two of different genotypes in the-se breeds, A/G and G/G. In Hanwoo, the A/G genotype frequency was 0.13, and G/G was 0.87. The CI of A/G was 382.18±10.03 days, and G/G was 381.69±5.22 days. The SPC of A/G was 1.62±0.16, and G/G was 1.32±0.04. While CI showed no significance difference, SPC exhibited significant difference (p<0.05). In Holstein cattle, the frequency of genotype A/G was 0.02 and G/G was 0.98. The 305 days milk yield of A/G was 7,253.00±936.00 kg and of G/G was 8,747.51±204.88 kg, showing no significant difference.

This study was conducted to determine the efficacy of anthelmintics against gastrointestinal parasitic infestation in periparturient dairy cows and its effect on milk yield and quality. Sixty pregnant cows of 1st & 2nd parity were divided into four groups. The efficacy of anthelmintic treatment was evaluated by counting faecal egg per gram (EPG) compared with pre-treatment values. The milk yield of each cow was recorded in pre and post treatment lactations. Cows of group A were treated with Nitroxynil 10 mg/kg body weight subcutaneously 30 days before parturition, group B were treated with combination of triclabendazole and levamisole 19.5 mg/kg body weight orally at calving and group C treated with Endex® at calving and 42 days after. The mean change in EPG 14 days after treatment was significantly higher (p<0.05) in treated (79.1%) cows than control. Average milk yield of group C (2.8 ± 0.8) was significantly higher (p<0.01) than group A (2.6 ± 0.7). Similarly, the average milk yield in all the treated cows was significantly (p<0.01) higher in treated lactation (2.5 ± 0.7) than in the previous lactation (2.2 ± 0.7). The average milk yield in all treated cows was significantly (p<0.01) higher than the control cows. Although, milk yields were higher in second parity than the first, there was no significant difference (p>0.05). The milk protein percentage was significantly higher (p<0.05) in treated group than the control group. Therefore, it may be concluded that periparturient anthelmintic treatment effectively reduced the gastrointestinal parasitic load and improved milk yield.

This study was conducted to investigate the effects of supplementing additional sucrose, in the form of black sugar (BS), into the diet of Holstein dairy cows on dry matter intake (DMI), milk yield, and milk composition. Eight Holstein dairy cows (741 ± 65.8 kg body weight) were divided into two groups, including the control and BS groups. Animals in the control group were offered a total mixed ration (TMR) ad libitum, and the BS group was offered TMR with 300 g of BS/head/d. After two weeks of adaptation period, the animal performance, including DMI, milk yield and milk composition, was measured. Cows supplemented with BS appeared to consume more feed than that by the controls (i.e., 17.08 and 18.28 kg/d for the control and BS groups, respectively). However, there were no significant differences between treatments. Milk yield or milk composition, such as milk fat, milk protein, lactose, solids-non-fat, total solids and pH, did not differ between treatments. However, there was a significant difference (p<0.05) in the concentration of milk urea nitrogen (MUN). The MUN concentration of the BS group was approximately 15% lower than that of the control group (i.e., 18.75 vs. 16.05 mg/dL for the control and BS groups, respectively), which suggests improved nitrogen metabolism in the animals. The somatic cell count was numerically lower in the cows of the BS group compared to those in the control group. However, a significant difference was not noted due to the substantial amount of variation among cows. In terms of the trace mineral composition for milk, the concentration of Cu from BS animals was higher (p<0.05) than that of the control animals. In summary, supplementing the diets of dairy cows with BS marginally affected animal performance and improved nitrogen metabolism. The level of supplementation and other factors, such as animal variation were discussed.

This study aimed to improve the reproductive efficiency of dairy herds by comparison and analyzing estrous appearance rate, conception and non-conception rate according to the stage of lactation using the lactation and reproductive records of average (less than 10,000 liters milk in 305 days) and high yielding (more than 10,000 liters milk in 305 days) Holstein cows (n=102). Milk production and reproduction data were collected between January 2010 and December 2012 from Holstein cows kept in the commercial dairy farms. Average (n=32) and high yielding (n=24) Holstein cows used to analyze the relationship between milk yield and reproductive performance. Our results showed that estrous appearance rate according to the stage of lactation was 25.0% (30∼59d), 40.6% (60∼ 89d), 25% (90∼110d) and 9.4% (>111d) in average yielding cows and 16.7% (30∼59d), 20.8% (60∼89d), 12.5% (90 ∼110d) and 50.0% (>111d) in high yielding cows, respectively. Conception rate according to the stage of lactation was 87.5% (30∼59d), 61.5% (60∼ 89d), 75.0% (90∼110d) and 66.7% (>111d) in average yielding cows and 25.0% (30∼59d), 0% (60∼89d), 33.3% (90∼ 110d) and 50.0% (>111d) in high yielding cows, respectively. Days between parturition and conception was 23.7% (<149d), 0% (150∼209d) and 0% (>210 d) in average yielding cows and 69.0% (<149 d), 77.8% (150∼209d) and 38.9% (>210d) in high yielding cows, respectively. Conception rate from 110 days postpartum in high yielding cows was 41.7% (110∼150d), 50.0% (151∼180d) and 50.0% (>181d). Body condition score (BCS) in 120 days postpartum was 2.64±0.1 in average yielding cows and 2.28±0.1 in high yielding cows, respectively.

본 시험은 포도부산물의 첨가가 젖소의 산유량, 유성분 및 혈액 성상에 미치는 영향을 조사하기 위하여 실시하였다. 비유중기 젖소 8두(평균 월령: 89.8, 평균 유기: 164.4, 평균 산차: 2.8산)를 공시하였으며, 포도부산물을 첨가하지 않은 대조구와 1일 1 kg을 첨가한 시험구로 두 처리구를 설정하여 3주간 급여하였다. 유지방, 유단백, 유당 및 총 고형물 함량은 처리간에 차이가 없었다. 그러나 산유량은 포도부산물 을 첨가한 처리구가 23.13 kg/d으로 대조구의 21.16 kg/d에 비해 높은 반면(p<0.05), 체세포 수는 대조구가 포도부산물 처리구에 비해 높았다(p<0.05). 혈중 glucose, alanine aminotransferase, total protein 및 blood urea nitrogen 함량은 처리구간 차이가 없었으나, AST와 총콜레스테롤 함량은 대조구 (123.75 IU/L, 200.82 mg/dl)가 포도부산물 처리구(95.34 IU/L, 180.63 mg/dl)에 비해 높았다(p<0.05). 이상의 결과에서, 다양한 생리활성을 가진 포도부산물은 젖소를 위한 기능성 사료로 이용 가능성이 높을 것으로 사료된다.

본 연구는 젖소에 있어서 삭제가 유 생산성에 미치는 영향을 구명하기 위하여 2003년부터 2004년까지 2년간에 걸쳐 축산연구소 개방형 깔짚 우사에서 사육중인 홀스타인 착유우 17두를 대상으로 처리구 9두 및 대조구 8두를 공시하였고 삭제는 분만 후 20일을 전후하여 1회에 한해 실시하여 다음과 같은 결과를 얻었다. 1. 유기에 따른 두당 1일 유량은 100일 이하의 경우 대조구 및 삭제구가 각각 및 , 일의 경우 및 , 201일 이상의 경우 및 으로

본 실험은 사료급여 종류가 동절기 우유의 생산량 및 조성분에 미치는 영향을 규명하기 위하여, 1998년 10월 부터 1999년 3월까지 실시하였다. 실험구 처리는 볏짚구(볏짚 위주 조사료＋농후사료: T1)를 대조구로 하여 옥수수 사일리지구(옥수수 사일리지 위주 ＋ 농후사료:T2) 및 TMR구(완전혼합사료: T3)를 상호 비교하였으며 그 결과를 요약하면 다음과 같다. 1. 우유 생산량은 T2(31.93.2kg) > T3(29.63.8kg) > T1구(22

대상방목 체계하에서 Lolium perenne초생의 채식 허용량을 3 수준으로 달리 하여 고능력 착유우에 의한 방목시험으로부터 얻은 채식량과 산유량을 조사하여 얻을 결과를 요약하면 아래와 같다. 채식된 사포로부터 배설된 분증질소, 크롬 및 회분 함량은 시험시기가 늦을수록, 채식허용량이 낮을수록 약간 감소되는 경향이었다. 시험시기별 채식 허용량의 수준에 따른 채식초의 소화율은 시험시기 1의 82.7%에서 시험시기 3의 79.1%로 감소하였고, 고수준의 채

본 연구는 한국종축개량협회(韓國種畜改良協會)에서 1985년-1989년 사이에 일반(一般) 낙농농가(酪農農家)를 대상으로 실시한 산유능력검정자료(産乳能力檢定資料) 4,008두(頭)의 기록(記錄)을 이용(利用)하여 유우(乳牛)의 주요경제형질중(主要經濟形質中), 유량(乳量)에 관여하는 환경요인중(環境要因中) 년도(年度), 분만연령(分娩年齡), 농가(農家), 착유(搾乳) 기간효과(期間效果)를 구명(究明)하기 위하여 수행되었으며 그 결과(結果)를 요약(要約)하면 다음과 같다. 1. 일반능력(一般能力)에 있어서 각(各) 형질(形質)의 평균치(平均値) 및 표준편차(標準偏差)는 실유량(實乳量)이 , 분만연령(分娩年齡) 개월(個月), 초산월령(初産月齡) 개월(個月) 그리고 착유기간(搾乳期間) 일(日)이였다. 12. 최소자승법(最小自乘法)에 의해 추정(推定)된 전체(全體) 분산(分散)에 대한 각(各) 요인별(要因別) 분산성분(分散成分)의 비(比)는 년도(年度)가 1.91%, 분만연령(分娩年齡)이 4.86%, 농가(農家)가 8.89%, 착유기간(搾乳期間)이 54.94% 그리고 오차(誤差)의 분산비(分散比)가 29.39%로 나타나 착유기간(搾乳期間)의 효과(效果)가 가장 큰 것으로 나다났다. 3. 유량(乳量)에 대한 전체의 최소자승효과(最小自乘效果) 추정치(推定値)는 6,229.31kg이였고, 년도별효과(年度別效果)에 있어서 최소자승(最小自乘) 평균치(平均値)는 1985년-1987년이 6,000.76kg, 1988년 6,028.11kg, 1989년 6,659.07kg이었다. 4. 분만연령별효과(分娩年齡別效果)에 있어서 24개월령이하(個月齡以下)는 5,456.01kg, 61-66개월령(個月齡)은 최고치인 6,565.48kg으로 분만년령간(分娩年齡間) 고도의 유의성(p<0.01)이 인정되었다. 이러한 분만연령별(分娩年齡別) 효과(效果)에 있어 24개월령이하(個月齡以下)에서부터 61-66개월령(個月齡)까지 점차 증가(增加)하다가 67-72개월령(個月齡)에 저조한 성적을 보이며 이상치를 나타냈고, 그 이후부터는 점차 감소(減少)되는 경향(傾向)을 보였다. 5. 농가별효과(農家別效果)에 있어서는 최소자승(最小自乘) 평균치(平均値)에 있어 가장 낮은 농가(農家)에서는 4,958.50kg에서 가장 높은 농가(農家)는 7,479.07kg으로 비교적 변이(變異)가 심하였고, 이는 고도(高度)의 유의성(p<0.01)이 인정되었다. 6. 착유기간별효과(搾乳期間別效果)에 있어서 착유기간(搾乳期間)이 경과(經過)함에 따라 최소자승평균치(最小自乘平均値)가 증가(增加)하는 면을 보였으며 착유기간(搾乳期間)에 따른 실유량평균간(實乳量平均間)에는 고도의 유의성이 인정되었다.

The present study was conducted to investigate effect of dietary protected amino acid on milk yield and composition in dairy cow using meta-analysis. Total 21 research papers were employed in analysis, and mixed model was used for the analysis of effects. Effect of protected methionine (PM) and combination of protected methionine and lysine (PML) were investigated under two different levels of dietary crude protein (CP, <18% and >18%). For performance of dairy cow, milk yield, milk composition including milk fat and protein content and yield and 4% FCM (fat corrected milk) production were used for analysis. In case of milk yield, a trend of increment was found at PM supplementation at low CP (P=0.055). However, the effect of PM at high CP was detected as not significant (P>0.05). In case of milk protein, inclusion of PM at low CP showed significant decrement (P<0.05). However, there was no significant effect of MP on milk protein at high CP (P>0.05). Supplementation of MP at high CP level showed significant increment of milk fat (P<0.05). MP supplementation represented significant increment of 4% FCM production (P<0.05) regardless of dietary CP levels. Effects of PML on milk yield and composition at both of low and high dietary CP were not significant in this study. However, it seem to be that there was a possible positive effect of MPL application at high dietary CP on performance of dairy cow.