The baculovirus expression system (BES) utilize the p10 or polyhedrin promoter, a very late promoter that exhibits strong transcriptional activity primarily at the end of viral infection, to produce useful recombinant proteins. The burst sequence of the very late promoter is essential for strong transcription, and VLF-1 is a transcription factor that binds specifically to the burst sequence, and it has been reported that it can regulate the amount and timing of expression of protein by the very late promoter. Recently, a VLF-1 constitutively expressing cell line was constructed to increase the production of the target protein, but the effect was minimal. In this study, to find the optimal VLF-1 expression conditions to increase target protein production efficiency, we controlled the expression of VLF-1 through various promoters and evaluated the target protein expression efficiency by the p10 promoter accordingly.

This study improved the work efficiency by supplementing the shortcomings of the manual process by developing a double tube feeding device, and the following results were obtained by conducting the production capacity, production length, and defect rate tests. Developed a double tube production system to enable the simultaneous production of two tubes, increasing the production volume by about 1.5 times. The product length has been improved from semi-automatic to automated, and the production capacity has been improved from 16 to 25 pieces per hour (based on 15m). Developed a double-tube input straight line automatic adjustment feeder, which resulted in reducing the defect rate to less than 1%.

Recently, in the case of the root industry, although it is a basic industry that forms the basis of manufacturing competitiveness, there continues to be a shortage of manpower due to reasons such as dangerous working environments, industrial economic difficulties, and low wage systems. In addition, the demand for automation of production lines using robots is increasing due to a shrinking labor market due to a decrease in the working population due to aging, higher wages, shorter working hours, and limitations of foreign workers. In this study, a system was developed to automate the injection molding process for producing ball valves for automobiles by applying robot system. The applied process flow consists of alignment and insertion of insert parts, and removal, transfer, and loading of the product after injection molding, which is currently performed manually. Through the application of the developed robot automation system, the cycle time was improved by more than 30% and the defect rate was reduced by more than 70%.

This study was conducted to investigate the growth characteristics of cucumber (Cucumis sativus L. ‘Joeunbaekdadagi’) and tomato (Solanum lycopersicum L. ‘Dotaerang Dia’) seedlings by light intensities and CO2 concentrations in a closed-type plant production system (CPPS). Cucumber and tomato seeds were sown in 50-cell trays and germinated in CPPS at air temperature 25 ± 1°C and relative humidity 50 ± 10% for 4 days. After germination, the CO2 concentrations and light intensity treatment were treated at 500 (ambient), 1,000, and 1,500 μmol·mol-1 and 100, 200, and 300 μmol·m-2·s-1 photosynthetic photon flux density (PPFD), respectively. The leaf area of cucumber showed the highest value in CO2 1,500 μmol·mol-1. However, the leaf area of the tomato had no significant difference in CO2 concentrations and light intensities treatments. In cucumber and tomato both seedlings, the growth and quality such as compactness and leaf area rate were increased with the increase of light intensity, and there were highest in 300 μmol·m-2·s-1. The root surface and number of root tips of cucumber and tomato seedlings were significantly increased with the increase in light intensity. In conclusion, the regulation of the CO2 concentrations and light intensity can control the growth and quality of cucumber and tomato seedlings in CPPS, especially, increasing the light intensity can improve more significantly the growth and quality of seedlings.

유기농 논이용 콩-밀 생산체계에서 재배유형별 밀 파종 전 경운여부에 따라 경운/무경운 처리, 콩 재배시 비닐 피복 여부에 따라 피복/무피복 처리, 논의 생태적 개선 여부에 따라 개선/대조구 처리로 시험구를 조성하여 밀 재배 중 곤충자원의 출현 및 분포특성을 조사 비교하였다. 출현 곤충의 총 종수는 20종으로 경운 14종, 무경운 14종으로 차이는 없었고 피복 14종, 무피복 16종이었으며 개선구 18종, 대조구 13종으로 개선처리에서 곤충 출현 종수가 많았다. 출현 곤충의 총개체수는 경운 124개체, 무경운 76개체로 경운처리에서 많았고, 피복 100개체, 무피복 100개체로 차이가 없었으며 경운-무피복-개선에서 40개체로 가장 많았다. 유기농 밀에 피해를 주는 해충 으로 노린재류는 갈색날개노린재가 가장 많이 발생하였고, 메추리노린재, 시골가시허리노린재도 출현하였으 며 재배유형별 발생 개체수는 경운 81마리, 무경운 39마리로 경운에서 많았고, 무경운-피복-대조(7) = 무경운-무 피복-대조(7) < 무경운-무피복-개선(9) < 경운-피복-개선(12) < 경운-무피복-대조(13) < 무경운-피복-개선(16) < 경운-피복-대조(27) < 경운-무피복-개선(29) 순으로 발생하였다.

표면발현(surface-display system)은 세포 또는 바이러스 표면에 목적 단백질을 고정하여 발현시킴으로써 목적 단백질에 대하여 독립적인 공간 구조 및 생물학적 활성을 부여하는 단백질 공학 기술이다. 또한 이를 이용하여 높은 중화항체 유도 및 대량생산이 가능한 삼량체의 형태로 항원 단백질의 발현 또한 가능하다. BES(baculovirus expression system)에서의 표면발현 기술은 번역 후 수정과정 및 복잡한 구조의 다양한 단백질의 발현이 가능하기 에 다른 숙주 기반 시스템보다 효율적이라고 보고되고 있다. 그러나 목적 단백질 외의 다른 표면 단백질과 발현 공간에서의 경쟁으로 목적 단백질의 낮은 생산량이 큰 문제점으로 지적되고 있다. 따라서, 이러한 BES에서 표면 발현의 생산 효율을 증대시키기 위하여, 동일한 표면 공간에 대한 단백질 간의 발현 경쟁에 대해 실험적으로 확인 후, 그를 해결하기 위하여 표면발현에 최적인 목적 단백질 발현을 위한 프로모터 선발 실험을 수행하였다. 이를 통해 BES에서 표면발현에 의한 목적 단백질의 생산 효율을 증대시킬 수 있음을 확인하였다.

본 연구는 DFT 재배 시스템을 이용한 밀폐형 식물생산시스 템에 MB처리 유무에 따른 상추(Lactuca sativa L.)와 배초향 (Agastache rugosa Kuntze)의 생육과 양액 특성을 조사하기 위해 수행되었다. 상추와 배초향은 밀폐형 식물생산시스템에 서 23일간 재배되었다. MB는 매일 9시, 13시, 17시에 5분씩 16일간 처리하였다. 상추와 배초향의 엽장, 엽폭, 엽면적, 생 체중 및 건물중은 MB처리구에서 대조구에 비해 유의하게 낮 았다. 상추와 배초향의 총 뿌리길이, 뿌리 표면적, 근단 수도 MB처리구에서 유의적으로 낮게 나타났다. 평균 뿌리 직경의 경우, 상추는 처리 간에 차이가 없었으나, 배초향은 MB처리 구에서 유의적으로 굵어 종 간에 차이가 있음을 확인하였다. 본 연구 결과, DFT 방식으로 상추와 배초향을 재배할 때 MB 처리는 비생물적 스트레스를 유발하여 생육을 억제하는 것으 로 나타났다.

In order to permanently dispose of radioactive waste drums generated from nuclear power plants, disposal suitability must be demonstrated and the nuclides and radioactivity contained in the waste drums, including those in the shielding drums, must be identified. At present, reliable measurements of the nuclide concentration are performed using drum nuclide analysis devices at power plants and disposal facilities during acceptance inspection. The essential functions required to perform nuclide analysis using the non-destructive assay system are the correction for self-attenuation and the dead time correction. Until now, measurements have mainly been performed for drums containing solid waste such as DAW drums using SGS calibration drums with ordinary iron drums. However, for drums containing non-uniform radioactive waste, such as waste filters embedded in cement within shielding drums, a separate calibration drum needs to be produced. In order to produce calibration drums for shielded and embedded waste drums, the design considered the placement of calibration sources, setting of shielding thickness, correction for medium density, and cement mixing ratio. Based on these considerations, three calibration drums were produced. First, a shielding drum with an empty interior was produced. Second, a density correction drum filled with cement was produced to create apparent density on the surface of the shielding drum. Third, a physical model drum was produced containing a mock waste filter and cement filled in the shielding drum.

글로벌 OTT 서비스의 등장과 급속한 확산은 기존 영상 콘텐츠 산업의 지형을 바꾸는 역할을 하고 있다. 이러한 OTT 서비스는 지역별 장벽이 존재했던 영상 콘텐츠 시장에서 콘텐츠 유통의 패러다임이 바뀌고 있다는 것과 콘텐츠 제작 영역에도 많은 영향을 주고 있다는 것을 보여준다. 결과적으로 영상 콘텐츠 산업은 제작, 유통, 소비에 이르는 산업 구조에 급격한 변동을 겪고 있다. 글로벌 OTT 사업자가 콘텐츠 유통과 소비의 중요한 부분이 되면서 나타난 중요한 제작 영역의 변화 중 하나는 전문적인 제작 능력을 갖춘 제작사들이 두각을 나타내고 있다는 사실이다. 이 논문에서는 글로벌 OTT 서비스의 급격한 확산으로 변화하게 된 제작과 유통 시스템에 대해 분석하면서 앞으로의 산업 동향에 대해서 정리하고 있다.

The International Atomic Energy Agency recommends the deep geological disposal system as one of the disposal methods for high-level radioactive waste (HLW), such as spent nuclear fuel. The deep geological disposal system disposes of HLW in a deep and stable geological formation to isolate the HLW from the human biosphere and restrict the inflow of radionuclides into the ecosystem. It mainly consists of an engineered barrier and a natural barrier. Safety evaluation using a numerical model has been performed primarily to evaluate the buffer’s long-term stability. However, although the gas generation rate input for long-term stability evaluation is the critical factor that has the most significant influence on the long-term hydraulic-mechanical behavior of the buffer, in-depth research and experimental data are lacking. In this study, the gas generation rate on the interface between the disposal canister and the buffer material, a component of the engineered barrier, was mainly studied. Gas can be generated between the disposal canister and the buffer material due to various causes such as anaerobic corrosion of the disposal canister metal, organic matter decomposition, radiation decomposition, and steam generation due to high temperature. The generation of gas in such a disposal environment increases the pore gas pressure in the buffer and causes internal cracks. The occurred cracks increase the intrinsic permeability of the buffer, which leads to a decrease in the primary performance of the buffer. For this reason, it is essential to apply the appropriate gas generation rate according to the disposal condition and buffer material for accurate long-term stability analysis. Therefore, the theoretical models regarding the estimation of gas generation were summarized through a literature study. The amount of gas generated was estimated according to the disposal environment and material of the disposal canister. It is expected that estimated values might be used to estimate the long-term stability analysis of buffer performance according to the disposal condition.

In the era of the 4th industrial revolution driven by the convergence of ICT(information and communication technology) and manufacturing, research on smart factories is being actively conducted. In particular, the manufacturing industry prefers smart factories that autonomously connect and analyze data. For the efficient implementation of smart factories, it is essential to have an integrated production system that vertically integrates separately operated production equipment and heterogeneous S/W systems such as ERP, MES. In addition, it is necessary to double-verify production data by using automatic data collection technology so that the production process can be traced transparently. In this study, we want to show a case of data-centered integration of a large aircraft parts processing factory that requires high precision, takes a long time, and has the characteristics of processing large raw materials. For this, the components of the data-oriented integrated production system were identified and the connection structure between them was explained. And we would like to share the experience gained through the design and implementation case. The integrated production system proposed in this study integrates internal components based on data, which is expected to serve as a basis for SMEs to develop into an advanced stage, and traces materials with RFID technology.

MMBR system has been suggested as a promising system to resolve harvesting problems induced from low settling efficiency of microalgae. And recently, a lot of research on reducing fouling at the MMBR system has investigated focused on EPS in many cases. EPS of microalgae mainly consists of polysaccharides and protein components, and is produced through photosynthesis and nitrogen-carbon metabolic pathways. Especially, P-EPS is one of major compounds which occur membrane fouling phenomenon, as its hydrophobic protein components cause floc formation and cake layer accumulation. And it is already known that almost every microalgae can metabolize P-EPS or Chl-a when nitrogen sources as a substrate is insufficient or exhausted situation. With the above backgrounds, uptake rates of P-EPS or Chl-a by Scenedesmus quadricauda according to the type of carbon source and nitrogen concentration were evaluated in order to verify correlation between carbon source vs P-EPS production, and indeed Scenedesmus quadricauda uses P-EPS or Chl-a when the amounts of nitrogen sourc es in the feed is not satisfied. As a result, it was shown that P-EPS and Chl-a production were increased proportional to nitrogen concentration under organic carbon condition. And especially, the amo unts of P-EPS and Chl-a in the cell were diminished with the nitrogen source becomes insufficient or exhausted. Because P-EPS accelerates fouling at the MMBR system, P-EPS degradation by Scenedesmus quadricauda in order to get nitrogen source may contribute to reducing fouling. About a affects of N-consumed Chl-a to the MMBR fouling, more survey is needed. On the contrary, considering the purpose of MMBR system of this study, i.e. harvesting useful high value microalgae efficiently feeding adequate industrial process wastewater, it seems like difficult to maintain satisfied metabolic activity and to harvest with high yield rate using nitrogen-poor MMBR feed.

본 연구에서는 ‘설향’ 딸기의 관부를 부분 난방하고 양액을 온수로 공급하면서 관행 재배 방식에 비해 온실 공간 온도를 낮게 관리하는 딸기 부분 난방 시험을 수행하였다. 정식 후 11월까지는 특별한 처리가 없어 대조구, 시험구 모두 온실 내 환 경이 유사하게 관리되었으며 관부 난방 및 온수 양액을 공급 하기 시작한 12월부터는 야간의 온실 온도, 관부 온도 및 베드 온도가 차이를 보였다. 12월의 온실 야간 평균 온습도는 대조구 7.1℃, 87.2%, 시험구 5.7℃, 88.7%로 시험구의 온도가 낮았으나 관부 난방을 수행함으로써 시험구 온실의 관부 및 베드의 온도를 9.3℃, 12.7℃로 유지하였고, 대조구 온실의 관부 및 베드 온도 7.9℃, 10.8℃보다 높게 관리할 수 있었다. 시험기간 내에서 시험구 온실의 딸기 관부 및 베드 온도는 대조구에 비해 모두 약 2.0℃ 가량 높게 유지되는 것으로 나타났다. 주간에는 온수 양액을 공급함으로써 지하수를 이용했을 때보 다 평균 8.7℃ 높은 온도 양액을 공급할 수 있었고 이로 인해 베드 온도도 약 5.0℃ 가량 높게 나타났다. 시험구에서는 시험 기간 동안 온실 난방, 관부 난방 및 온수 양액 공급에 총 9,475.7×10 3 kcal의 에너지를 소비하였고 대조구에서는 온실 공간 난방에 총 16,847×10 3 kcal의 에너지를 소비하여 시험구에서 대조구 대비 약 43.8%의 에너지 절감 효과를 확인할 수 있었다. 시험구에서 딸기 관부의 온도를 높게 관리함으로써 작물의 생육을 촉진시킬 수 있었고 그로 인해 정식 후 초세가 대조구에 비해 좋지 않았던 시험구의 딸기가 25주 후에는 대조구와 생육적인 면에서 큰 차이가 없는 것으로 나타났다. 딸기의 수확량은 초세가 좋았던 대조구에서 1화방의 수확량이 시험구에 비해 많았으나 2화방, 3화방에서는 관부의 온도를 높게 관리한 시험구의 수확량이 더 많았다. 3월 말까지의 주당 수확량은 시험구 412.7g/plant, 대조구 393.3g/plant로 유의미한 차이는 없었으나 시험구가 대조구에 비해 4.9% 많이 나온 것으로 보아 딸기의 온도 민감부인 관부의 온도를 높게 관리하는 것이 딸기 생육과 생산성에 영향을 미치는 것으로 판단된다.