본 연구는 ICR-mouse를 실험군당 5마리를 공시하여 식이에 산벚나무 씨앗 기름 및 옥수수 기름을 첨가하여 배합한 사료의 섭취가 쥐의 혈중 지질 수준에 미치는 영향을 비교․ 분석하였다. 1. 산벚나무 씨앗에는 palmitic acid, oleic acid 및 linoleic acid 등이 함유되어 있으며, linolenic acid와 erucic acid가 소량 함유되어 있다. 2. 산벚나무 씨앗 기름의 항산화 값(DPPH)은 4.63%로 나 타났으며, 신장의 무게는 다른 두 군에 비해 산벚나무 씨앗 기름을 섭취한 군(1.24±0.22 g)에서 유의적으로 높았다(p<0.05). 3. 산벚나무 씨앗 기름을 섭취한 군에서 HDL-cholesterol 농 도는 가장 높았으며, 혈중 LDL-cholesterol 농도는 가장 낮았 다(p<0.05). 이상의 결과를 정리해 보면, 산벚나무의 씨앗 기름의 지방 산 조성은 다른 식물성 씨앗 등의 지방산 조성과 큰 차이가 없었으며, 혈중의 HDL-cholesterol 농도를 높여주는 반면에, LDL-cholesterol 농도는 낮추어 주는 것으로 나타났다. 따라서 산벚나무의 씨앗 기름을 현재 유지 자원으로 사용되고 있는 참깨나 들깨와 같이 식용유지 자원으로 개발하기 위해서는 Choi 등(2012)의 연구에서 지적했듯이 씨앗 기름의 항산화 효과, 산패 진행 정도 및 인체에 미치는 영향 등의 지속적인 연구가 이루어져야 한다고 사료된다.

Background: In this study, we investigated the antibacterial and nitric oxide (NO) production inhibitory activities of 75% ethanol extract of Prunus sargentii branches and its fractions against acne pathogens. Methods and Results: The antibacterial activity against acne causing pathogens was determined using the disc diffusion assay. The ethyl acetate fraction showed higher activities against Propionibacterium acnes, Staphylococcus aureus and Staphylococcus epidermidis than those shown by other fractions. In the DPPH radical and NO scavenging assays, the butanol fraction showed strong DPPH radical and NO scavenging abilities. These activities were related to the total polyphenol and flavonoid contents of butanol fraction. On the other hand, the chloroform and ethyl acetate fractions exhibited the highest NO production inhibitory activity in Lipopolysaccharide (LPS)-stimulated Raw 264.7 cells compared to those exhibited by other fractions. Conclusions: The extract and its ethyl acetate fraction from the branches of P. sargentii exhibited antibacterial activity and could be used as functional materials in antimicrobial related fields. Moreover, the chloroform and ethyl acetate fractions are potential antiinflammatory agents and butanol fraction acts as an effective radical scavenger.

This study was carried out to investigate the antibacterial activity against pathogens of acne and the anti-inflammatory effect of 75% ethanol extract and its fractions from the leaves of Prunus sargentii. In the antibacterial activity by the disc diffusion assay, the extract showed the highest effect against Propionibacterium acnes, Staphylococcus aureus and Staphylococcus epidermidis in 5 mg/disc. However, the ethyl acetate fraction showed the highest antibacterial activity in 1 mg/disc. On the other hand, the hexane and chloroform fraction showed strong nitric oxide (NO) production inhibitory effect in lipopolysaccharide (LPS)-stimulated Raw 264.7 cell. In the cell viability of Raw 264.7 by MTT assay, the extract and all fractions were exhibited normal viabilities as nontoxic result. Consequently, the extract from the leaves of P. sargentii and its ethyl acetate fraction could be applicable to functional materials for antibacterial activity related fields. Moreover, the hexane and chloroform fraction could be applicable to candidate materials as anti-inflammatory agent.

In this study, we investigated the antibacterial activity, antioxidative activity and whitening effect of 75% ethanol extracts from different parts of Prunus sargentii. The total phenolic compound content of the branch extract was 277.92 mg/g as the highest level. In the measurement of DPPH radical scavenging ability, SC50 values of the cork layer and branch extract were 26.79 μg/ml and 30.13 μg/ml. In nitric oxide (NO) scavenging ability, SC50 values of the branch and leaf extract were 49.19 μg/ml and 55.55 μg/ml. All extracts exhibited higher NO scavenging ability than ascorbic acid used as positive control. On the other hand, in antibacterial activity against Staphylococcus epidermidis and Staphylococcus aureus by disc diffusion assay, the pure bark extract showed the highest activity. Moreover, tyrosinase inhibitory activity of cork layer, pure bark and branch extracts showed higher activity than arbutin used as positive control. In the cytotoxicity measurement by MTT assay, leaf extract was exhibited Raw 264.7 cell viabilities of 44.68~61.83% as cytotoxic result in tested concentration. In conclusion, the branch extract of Prunus sargentii will be a functional materials without damage compared to other parts such as pure bark or cork layer in the plant.

Prunus sargentii of Rosaceae familiy, has been reported to have radical scavenging activity and anti-inflammatory effect. On these facts, this research was conducted to evaluate pharmaceutical activities of the bark extracts P. sargentii. Free radical scavenging activities of fraction (Fr) -5~10 isolated from P. sargentii was higher than 80% respectively at 10ppm. The superoxide dismutase (SOD)-like activity of Fr-5, 9 were about 97, 84%, respectively at 1,000 ppm. Xanthine oxidase inhibitory effect of Fr-9, 10 were about 75, 78%, respectively at 1,000 ppm. The tyrosinase inhibitory effect related to skin-whitening was 72, 68% in Fr-2, 9 isolated from Prunus sargentii R. at 1,000 ppm. Hyaluronidase inhibition activity related to the anti-inflammation effect was 98% for Fr-8 at 500 ppm. Isolation of the methanol soluble fraction from P. sargentii yielded two major phenol compounds, (-)-epicatechin and taxifolin. The structure of the compound was certainly determined by chemical analyses, as well as NMR and Mass spectroscopy. The present study was carried out in a search for new cosmetic material from the bark from P. sargentii. and, (-)-epicatechin and taxifolin were isolated as active principles. So P. sargentii R. methanolic extracts may be used for the cosmetic material.