This study was conducted to investigate the optimum supplementation ratio when replacing cotton seed meal with rapeseed meal for cultivating oyster mushrooms. The chemical properties among the treatments were pH 4.5~4.7, total carbon content was 46.3~46.5%, total nitrogen content was 1.6~1.7%, and carbon-nitrogen ratio was 27.0~27.8. These did not differ significantly from the control. Mycelial growth was 85.1 mm (Heuktari), and 72.8 mm (Suhan-1Ho) in medium containing 5% rapeseed meal, with no significant difference in mycelial density between cultivars. Fruiting body characteristics are as follows: In 'Heuktari', the color of the pileus was darker than control with an L value of 32.5, and pileus thickness and diameter were 3.2 mm and 27.6 mm, respectively. Stipe length and diameter were 86.0 mm and 9.1 mm, respectively. Valid stipe number per bottle (1,100 cc) was 25.9, and a yield of 189.7 g/1,100 cc was recorded in mixed medium containing 5% rapeseed meal (higher than control). In mixed medium containing 10% rapeseed meal, the yield was 184.5 g/1,100 cc (comparable to control). In 'Suhan-1Ho', supplemented with 5% rapeseed meal, pileus color was darker than control color, with an L value of 28.8. Pileus thickness was 4.5 mm. Stipe diameter was 12.3 mm (higher than control), and yield was 145.5 g/1,100 cc (comparable to control).

Potato is an important cash crop of Pakistan and widely cultivated in plains and mountains of Punjab. Khyber Pakhtun Khawa Gilgit Baltistan, on an area of 0.313 million hectares in summer, autumn and spring with an annual production of 07.9 million tons. In Pakistan potato yield is 25.2 tons per hectare, which is although above the world average but comparatively low as compare to the potential and other advance potato growing countries because of several reasons. Availability of healthy seeds and advance production technology are the major constraints to achieve the higher potato production goals. It is very alarming that in spite of good position in potato growing countries, we are unable to produce good quality seed. In Pakistan >01% quality seed is available against the seed demand around 0.5-0.7 million tons per annum and mostly depend upon the imported seed 15000-20000 tons per annum. Tissue culture industry has been a proven global technology in potato seed production, so it is dire need of the growing population and climatic change to adopt these modern technologies of potato production for sustainable and higher production and income to contribute potato growing farmers of Pakistan. It is possible with the commercial use of tissue culture and allied techniques as aeroponic potato seed production along with good management and plant protection measures to provide indigenous high vigor and high yield potential potato seed. Pakistan Agricultural Research Council has established Aeroponic Potato Seed Production facility at National Agricultural Research Center with the collaboration of Korean Govt RDA-KOPIA Pakistan. The Aeroponics is a modern technology for growing plants by providing a nutrient solution in the air without soil. Plants grow rapidly in the growth chamber under the aseptic environment, balanced nutrition and sufficient oxygen availability that improves potatoes production in the aeroponic system. This can produce 10 times more yield than conventional production systems. Aeroponics techniques are credited for making potato yield more efficient, and can reduce the number of steps in the potato seed multiplication. Through tissue culture and aeroponics facilities development in Pakistan, first ever more than 200,000 nucleus mini harvested at NARC from the autumn 2021 and spring 2022 planation at KOPIA screen houses. Further seed multiplication from the harvest is under progress at up hills and more than 500,000 tubers (G1) are expected to be harvested during October-November 2022. It is very encouraging, impressive and successful venture of KOPIA at Pakistan for the potato seed production and self-sufficiency to insure food security in the country.

Zanthoxylum schinifolium is an important short-term income species and a useful resource with various physiological activities. In this study, the distribution and characteristics of seed-rich individuals of Z. schinifolium trees were analyzed. Seed-rich individuals were selected from three regions in consideration of seed yield and growth factors. As a result of the leaf shape survey, the length and width of the leaves were shorter in Jeju and longer in Geochang and Hadong compared to the average. There were very large differences in seed production between individuals within the Z. schinifolium group. The amount of seeds was higher in Jeju and Hadong than in Geochang. As a result of analyzing the correlation between the seven morphological characteristics, the overall correlation coefficient was in the range of -0.910 ~ 0.933. Total seed mass (TS) and seed weight (SW) showed a strong positive (+) correlation. As a result of the principal component analysis, it was classified into two categories. The first principal component showed a high correlation in the order of total seed weight and total seed amount, and the second principal component showed a high correlation with seed length and tree height. As a result of comparing seed production for two years, it was found that there was no change in production. As a result of examining seed viability using the TTC method, all of Z. schinifolium seeds showed more than 90% vitality. It is judged that the above results will serve as basic data for the development of a new variety of seed-rich Z. schinifolium trees.

Cassia tora seed was processed by germination and roasting to develop a germinated Cassia tora tea. The effects of extraction temperature on antioxidative compounds in tea extracts were examined and compared to four different commercial Cassia tora tea products. The contents of antioxidative compounds, such as phenolics, flavonoids, and β-carotene, increased as the extraction temperature increased (5 to 80oC), subsequently increasing in vitro antioxidant activities (DPPH and ABTS radical scavenging activities). Germinated Cassia tora tea had higher antioxidative activity than selected commercial Cassia tora teas, and the difference was significant at low extraction temperature. Therefore, the germinated Cassia tora tea could contribute to a new type of tea product with enhanced bioactive and antioxidative activities by adopting appropriate processing and extraction conditions.

양파 종자는 양파 생산을 위한 기초적 투입자원이기 때문에 건전한 종자는 양파 생산에서 매우 중요하다. 양파 종자에서 분리한 박테리아 중에서 양파 종자 발아를 억제하는 균주 OS1을 분리하였다. 분자 동정을 위해 16S rRNA 유전자 영역의 DNA를 분석하였고 이를 바탕으로 한 유연관계 분석을 통해 OS1 균주를 Leclercia adecarboxylata로 동정하였다. L. adecarboxylata는 면역 저하 환자 또는 기저 질환이 있는 환자에게 기회감염을 일으키는 것으로 알려져 있다. L. adecarboxylata OS1은 휘발 물질을 생산하고 이 휘발 물질이 양파 종자 발아를 억제하는 것으로 분석하였다. 특히, 양파 구근을 이용한 병원성 검정에서도 L. adecarboxylata OS1는 갈변증상과 썩음증상을 유발하였다. 아직 L. adecarboxylata에 의한 대규모 포장 또는 수확후병으로 인한 양파 피해사례가 보고된 적은 없지만, 이러한 결과는 L. adecarboxylata는 잠재적인 양파 병원체가 될 수 있음을 시사하고 양파 생산에 잠재적 위협 요소가 될 가능성을 보여준다. 또한, L. adecarboxylata는 인체 기회감염 병원체이기 때문에 양파로부터 언제든지 인체로 감염될 수 있는 위험성을 갖고 있다고 볼 수 있다.

Iris laevigata, which belongs to the Iridaceae, is now designated as an “endangered” (EN) grade by Korea Forest Service because it does not have many natural sites known for its reckless development and damage to its natural habitats. This study was carried out to establish the propagation protocol from seed to restore the native habitat of the I. laevigata and to utilize it for ornamental purposes. Basically, the appearance and internal structure of seeds were observed and imbibition experiments were conducted. Germination rate was measured by cold stratification experiment, after warm followed by cold stratification experiment, and GA3 treatment experiment. The seeds had underdeveloped embryos, which had grown to about 25% of those of fully matured seed before germination. In the controlled laboratory experiment, after cold stratification at 5°C for 0, 4, 8, or 12 weeks, the seeds germinated to 0, 11.7, 43.4, or 51.7%, respectively, after 4 weeks of incubation at 25°C. After warm stratification (25°C, 8 weeks) followed by cold stratification for 0, 4, 8, or 12 weeks, the seeds germinated to 0, 51.7, 85.0, or 88.3%, respectively, after 4 weeks of incubation at 25°C. GA3 treatment did not overcome the dormancy. Our study determined the dormancy type of I. laevigata seed. Imbibition experiments showed that there was no physical dormancy, and it was also found that there was an underdeveloped embryo when it was observed that the embryo was growing according to the period of incubation. A nd t he e m bryo grew a t relatively w arm temperatures. It is concluded that the seeds of I. laevigata have morphophysiological dormancy (deep simple MPD). This is the first report to determine the dormancy type in seeds of this valuable ornamental plant.

This experiment was conducted to investigate the change in the productivity of Italian ryegrass seeds according to the inter-row spacing in the southern region of the Korean Peninsula. Italian ryegrass (Lolium multiflorum Lam.) ‘Green Call’ variety was sown in Jinju, Gyeongnam in the fall of 2020 with three inter-row spacings (20, 30 and 40 cm). The experiment was arranged a randomized block design with three replications. The ryegrass was sown on October 17, 2020, and the harvest was on May 31, about 60 days from the first heading stage. There was no difference among treatments with an average of April 27th in heading stage. Plant height was significantly longer at 30 cm seeding interval and the shortest in 20 cm treatment. The length of the spike was the longest in the 40 cm seeding interval, and the number of seeds per spike was the highest in the 20cm seeding interval, but there was no significant difference among treatments. The seed yield was the highest at the 20 cm sowing interval (2,180 kg/ha), and decreased as the spacing increased. The dry matter content of seeds and straw was found to be 44.90% and 45.51% on average, and there was no significant difference among treatments. The amount of remaining straw after harvesting was found to be 7,506 kg/ha on average on DM basis, and was high at the 20 cm seeding interval. In view of the above results, it was found that it is most advantageous to sow at intervals of 20 cm when producing Italian ryegrass seeds through autumn sowing in the southern region.

본 연구는 2018, 2019, 2020년 논에서의 배수 깊이에 따른 식용(일미찰), 사료용 옥수수(광평옥) 그리고 장류콩(대원콩) 및 나물콩(해품콩)에 대한 생육 특성과 수량 구성요소를 비교 검토하고자 수행하였다. 논의 배수로 폭은 80cm로 고정하고 깊이를 20cm과 40cm로 각각 달리하였다. 연차간에 있어서 일미찰은 간장, 착수고, 이삭무게 이삭착립장 및 이삭 수량이 고도의 유의성이 나타났으며, 광평옥도 일미찰과 유사한 반응을 보였다. 배수로 깊이에서는 40cm가 20cm보다 수량이 고도로 유의하게 높았다. 한편, 연차간 해품콩은 간장, 분지, 꼬투리 수, 미 성숙 종자률, 100립 중 및 종실 수량에 고도의 유의한 차이를 보였다. 반면, 대원콩은 간장, 100립중, 수량간에 고도로 유의하였다. 배수로 깊이 간에는 해품콩과 대원콩의 수량은 통계적 유의한 차이는 나지 않았지만 20cm보다 40cm에서 수량이 약간 높았다. 본 연구 결과 옥수수의 수량은 배수로 깊이에서 따라 통계적인 고도 유한 차이를 보였으나 콩은 통계적인 차이는 나타나지 않았으나 40cm이 20cm보다는 높았다. 한편 콩은 미성숙 종자률은 배수로 깊이에 효과적으로 차이를 보였다. 따라서 논에서 옥수수 또는 콩을 재배시 가장자리에 20cm 배수로 보다는 40cm 배수로 설치를 권장한다.

Bulbophyllum auricomum Lindl. is a rare orchid and has flowers with an attractive fragrance. The present study investigated the tissue culture method for micropropagation. Capsules derived from artificial self-pollination were obtained for the best seed germination in MS basal medium. Plant growth regulators (1.0 mg·L-1 of BAP and 2.0 mg·L-1 of NAA) were affected by callus induction from subcultured pseudobulb explants. For the callus subculture, different natural plant extracts were tested in 11 treatment media. Among them, MS medium with 150 mL·L-1 of coconut water was generally effective in fresh weight (1.75 ± 0.08) and (3.01 ± 0.20) of callus proliferation and PLBs induction at 1 and 2 months, respectively, followed by an MS combination of 30 g·L-1 of banana and 20 g·L-1 of potato extract. The results of a comparative study of different MS mediums containing plant growth regulators with a natural extract combination and MS medium supplemented with natural extract only showed that MS medium supplemented with a combination of natural extracts (150 mL·L-1 of coconut water) and plant growth regulators (2.0 mg·L-1 of BAP and 1.0 mg·L-1 of NAA) obtained the highest shoot regeneration (3.37 ± 0.17) and (6.41 ± 0.68) after 1 month and 2 months of culturing, respectively.