점박이응애(Tetranychus urticae)는 250과 1400여종의 식물에 피해를 주고 있는 해충으로 높은 번식력, 근친 교배, 무성생식, 짧은 수명주기 로 년간 발생회수가 많기 때문에 약제에 대한 저항성이 빠르게 발달하는 종이다. 저항성 기작은 AChE 저해, sodium channel modulator, glutamate gated chloride channels allosteric, mite growth inhibitors, inhibitors of acetyl CoA carboxylase, mitochondrial ATP synthase 저 해, mitochondrial complex I, II, III, 그리고 electron transport 저해 등이 있으며 약제에 따라 작용기작이 다르므로 약제살포 시 확인이 필요하 다. 약제저항성 발달을 평가하는 독성시험방법으로 slide dip, leaf dip, leaf disc, topical application, vial leaf dip, spray potter tower, leaf vial dipping 등이 있으며 최근에는 분자학적 진단법 등도 개발되어 있으나 이들 방법은 해충 종이나 발육상태에 약제에 따라 정확도가 달라지므로 현장 에 가장 맞는 방법을 찾아야 할 것이다. 저항성 점박이응애를 효과적으로 관리하기 위한 방법으로 저항성 위험이 낮은 살응애제(chemical acaricide) 약제 선발, 천적인 칠레이리응애, 사막이리응애를 이용한 생물적 방제, 님, 제충국, 등 식물추출물이나 정유를 활용한 친환경적인 방법 등이 제시되 고 있으며 종합관리를 위한 다양한 요인을 찾아 약제 사용을 줄여 나가야 할 것이다.

A poorly known spider, Pholcus kwangkyosanensis Kim & Park, 2009 is redescribed with diagnosis, detailed descriptions, and taxonomic photographs of diagnostic characters. P. kwangkyosanensis is similar in appearance to P. kwanaksanensis Namkung & Kim, 1990, in terms of the shape of the genital organ and body, but it can be easily distinguished from the latter by the shape of the cheliceral apophysis, uncus, and procursus in males, as well as the shape of the epigynum and pore plates in females. The specimens of this spider were collected at the type locality (Mt. Gwanggyosan, Suwon), specifically under the bridge on a local stream in an agricultural landscape surrounded by rice fields, upland fields, or horticultural greenhouses.

이 연구는 애니메이션 <스파이더맨: 뉴 유니버스>에서 색채 심리학 이 캐릭터 형성과 서사에 미치는 영향을 탐구한다. 연구는 색채가 애니 메이션 영화에서 감정 전달, 캐릭터 개성 형성, 이야기 발전에 대한 잠 재력을 밝히려고 한다. 정성적 분석 방법을 사용하여 영화 내 색채 사 용을 해석하고, 특히 색채가 캐릭터의 감정 표현과 관객의 감정 반응에 어떻게 영향을 미치는지 조사했다. 연구 결과는 <스파이더맨: 뉴 유니 버스>에서 색채 사용이 캐릭터 이미지의 깊이와 감정의 풍부함을 증진 시키고, 서사의 동적성과 시각적 매력을 강화함을 보여준다. 색채 변화 를 통해 영화는 감정과 주제를 성공적으로 전달하고, 관객의 감정 공명 과 이야기 이해를 증진한다. 색채의 전략적 사용은 캐릭터 간의 관계와 이야기의 핵심 전환점을 강조하여, 시각적 서사에서 색채의 필수적인 역할을 보여준다. 이 연구는 애니메이션 영화 제작에서 색채 심리학의 적용을 더 깊이 탐구할 필요성을 강조하며, 향후 연구에서 정량적 연구 방법을 사용하여 색채가 관객 인식과 감정 반응에 미치는 영향에 대한 실증 데이터를 수집할 것을 제안한다. 동시에, 문화적 배경과 개인 경 험이 색채 인식에 미치는 영향을 고려한 개인 차이를 더 깊이 탐구할 필요가 있다.

In contrast to conventional silk fibroin, spider silk's potential as a scaffold material for tissue engineering is examined in this work. The remarkable qualities of spider silk are being researched for use in making films for tissue regeneration. In comparison to silk fibroin films, the study's analysis of orb-web spider Trichonephila clavata films highlights their improved cell adhesion and nanofibrous network structure. Tests for solubility substantiate the durability of spider silk films, while in vitro investigations demonstrate low cytotoxicity and enhance cellular viability. The conclusion highlights the exceptional properties of spider silk, which make it a viable option for tissue engineering applications and a step forward for in vitro cell culture and regenerative bioengineering.

Spider silk, which combines strength and lightness, emerges as an ideal candidate for non-woven fabric production. Unlike synthetic polymeric materials, silk are nontoxic and biocompatible and cause no allergic reaction. In particular, the dragline silk produced by the golden orb-web spider, Trichoephila clavata, is renowned for its superior strength and exceptional mechanical properties compared to other spider silks. Non-woven fabrics have found extensive applications in diverse industrial sectors, owing to their advantageous qualities such as breathability, durability, and lightweight characteristics. Natural dragline silk was extracted from the orb-weaving spider and subsequently randomly arranged. Compressing into a sheet-plane form, in addition to inducing the formation of water-resistant, stable β-sheet structures through ethanol vapor treatment or direct treatment, preserved the morphology of the silk fibers. The resulting non-woven sheets were analyzed with an field emission scanning electron microscope (FESEM) to observe their fine structures and mesh size were measured through image analysis.

Transmission electron microscopy was used to examine the microscopic structural features and myofibril organization of cardiac muscle cells in the orb-web spider T. clavata. There are many myofibrils, many mitochondria, a large sarcoplasmic reticulum, and transverse tubules (T-tubules) in the muscle fibers, even if the myofibril striations may not be as noticeable as in skeletal muscles. Because of their consistent striations, sarcomeres are characterized by Z-lines that are 2.0 μm on average in length and do not clearly distinguish between the A- and I-bands. A single T-tubule paired with a terminal cisterna of the sarcoplasmic reticulum constitutes a dyadic junction, which is primarily located at the A-I level of sarcomeres. Cells are joined by intercalated discs, which create several linkages via specialized junctions such as desmosomes, gap junctions, and fascia adherens—all of which are essential for heart function. Our results with transmission electron microscopy (TEM) clearly show that the contraction of the spider's heart muscle is neurogenically controlled, since each fiber is innervated by a motor neuron branch via neuromuscular junctions. These results highlight the neurogenic process controlling spiders' cardiac muscle contractions and advance our knowledge of the peculiar cardiac muscle structure of these animals.

The genus Argiope are known to decorate their webs with various patterns. The purpose of these web decorations, referred to as stabilimenta, is still a subject of debate. They are believed to serve either as lures to attract prey insects or as a means of providing protection to the spider by enhancing the web's visibility to deter potential predators. In this study, we observed spider orientation with respect to the direction of the sun in a controlled laboratory environment. To assess the orientation preference of the spider, the frame was repositioned daily by reversing its direction. Over a period of 21 days, the orientation of the spider was meticulously recorded, distinguishing between the illuminated side (open side) and the shaded side (closed side). field emission scanning electron microscopy (FESEM) visualization were used to investigate the microstructural characterization of stabilimenta in both juvenile and adult spiders. Our results represent that the decorative structure of stabilimentum shows diverse microstructural features according to the maturation level of the spider. In addition, it has been confirmed that spiders tend to prefer the open side direction when stabilimentum is present.

This study investigated the distribution and diversity of spider communities living in natural and semi-natural environments located in Gongju, Chungcheongnam-do, South Korea, and thereby emphasizes the importance of urban planning and biodiversity conservation. In this study, we documented 128 spider species through pit-fall trapping, sweeping, and manual collection methods and analyzed their alpha and beta diversity, nestedness, and interactions. As a result, natural habitats (forests) showed higher biodiversity and more complex species interactions. Despite anthropogenic impacts, such as human activities, urban habitats have shown notable biodiversity. Nestedness analysis and association rule learning reveal diverse community structures in multiple habitats and intricate interspecies relationships. This study highlights the importance of habitat heterogeneity in supporting biodiversity and the importance of considering multiple ecological factors in urban planning.

Two-spotted spider mite (Tetranychus urticae Koch; TSSM) is an agriculturally serious pest tat has acquired strong resistance against acaricide. Alternatively, mite-pathogenic fungi could be used to control the mites. The spider mite has symbiotic microorganisms which could be involved in the physiological and ecological adaption to biotic stress. In this study, mite-pathogenic fungi were used to control female adults, and the change of microbiome in the fungus-infected mites was analyzed. The acaricidal activity of fungal isolates was tested, and Akanthomyces attenuatus JEF-147 with the highest acaricidal activity was determined, followed by semi-field assay. Microbiome in the female adults which was infected by A. attenuatus JEF-147 was analyzed, and composition of microorganism was changed by fungal treatment. In bacteria abundance, the arthropod defense-related Rickettsia increased, but reproduction-associated Wolbachia decreased. The change in major bacterial abundance in the infected mites could be explained by the trade-off between reproduction and immunity. This work describes that a possible trade-off in arthropods against fungal pathogens could be predicted by the microbiome analysis.

The series compounds from natural products are an effective repellent and deterrent against various kinds of pests. In this study, we evaluated the spatial repellency of fifteen compounds from natural products on the two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae), in the laboratory and field by using two-choice and no-choice bioassays. In laboratory two-choice tests, six compounds displayed active spatial repellency against female adult mites at a 2mg dose. The repellency of each compound was also as effective as the 6-compound blend. Three of the six compounds showed the predominant repellent activity (over 90%) that lasted for at least 3 days in laboratory no-choice tests. In a field test, we found that the number of T. urticae was fewer in strawberry seeding treated lure with 2mg of these compounds than in strawberry seeding treated lure with solvent control. Given that the findings are efficacious, economical, and natural products, they can be used in the sustainable management of T. urticae in greenhouse.

Two species of the genus Oxytate L. Koch, 1878 have been recorded in Korea to date. The spider fauna was surveyed in the mountainous terrain of Mt. Hallasan National Park in 2019. A female of Oxytate lobia sp. nov. was collected with a sweep net between shrubs in mixed forests during a seasonal spider survey and is described with measurements, morphological illustrations, and a diagnosis. The new species can be easily distinguished from similar species within the genus by the long copulatory duct conjugated with a spermatheca, a pair of triangular pits at the bottom, and no ditches.