계란의 비린내 강도는 가금류의 종류와 개인의 인식에 따라 다르지만 여러 요인에 의해 발생할 수 있다. 특히 트 리메틸아민(TMA)이 원인으로 밝혀졌다. 주목할만한 점은 리보플라빈이 TMA를 무취의 트리메틸아민-N-옥사이드로 전환시키는 역할을 하는 효소인 플라빈 함유 모노옥시게 나제 3의 활성을 증가시킬 수 있다는 점이다. 본 연구는 난황의 TMA 함량을 분석하여 비린내에 대한 기여도를 평 가하고, 비린내를 방지하는 방법을 개발하는 것을 목적으 로 하였다. 고체상 미세추출-기체 크로마토그래피/질량 분 석법을 사용하여 리보플라빈이 강화, 보충된 사료를 먹인 암탉의 난황에 있는 휘발성 화합물을 감지하고 정량화했다. 또한 샘플 간 휘발성 물질의 상대적 함량을 비교하기 위해 전자코를 이용하여 상관관계 연구를 수행하였다. TMA는 콜린이 함유된사료를 섭취한 가금류의 난황에서 고농도로 검출되었지만 리보플라빈이 보충된 사료를 섭취한 가금류 에서는 검출되지 않았다. 전반적으로, 이 연구는 리보플라 빈이 TMA를 포함하여 계란에 존재하는 휘발성 물질의 양 과 품질에 영향을 미친다는 것을 시사한다. 이러한 발견 이 계란의 비린내를 줄이는 것은 물론 품질 향상에 기여 하기를 기대한다.

The experiment was performed for establishing the best condition of experiment and defining the issue of GC/FID methods based on HS-SPME-capillary column when TMA is measured in the air. Also the experiments are performed based on odor process test method alternately. At first, The result that KOH and NaOH were compared for eluting the TMA among the preprocessing processes, showed that NaOH is 2.2 times higher figure of area, 0.9972 of curve linearity(R2), and 1.96% of relative standard deviation. As a result using the ultrasonic cleaner and 30℃ constant temperature water bath, constant temperature water bath showed stabilized curve linearity (R2 0.9972) and relative standard deviation (RSD 2%). In advance of experiment conditions, the result performed acid filter paper method and acidic solution absorption among TMA sampling methods, is that acidic solution absorption detected 5 times better sensitivity than Acid filter paper method. Acidic solution absorption showed to provide excellent analysis sensitivity in this experiment restrictively. Therefore, in case of experiment by GC/FID method based on HS-SPME-capillary column, selection of sampling method expects that acidic solution absorption better effect and in case of disassemble reagent, NaOH and 30℃ constant temperature water bath is better effect in the experiment conditions.

In this study, a series of experiments were carried out to measure temporal stability of trimethylamine (TMA) in two different bag sampler types (polyvinyl fluoride bag (PVF) and polyester aluminum bag (PEA)) as a function of storage time. To this end, temporal variability of TMA was measured using its standard gas samples up to 4 days. For the purpose of comparison, storability of TMA was tested between PVF and PEA. To this end, standard gas samples were prepared at two concentration levels of 1 and 10 ppm. The results of this study indicate that the reduction of TMA levels generally increased with the extension of the storage time. Although both bag types showed moderate losses of TMA, differences between bag types (PVF and PEA) were likely to be less significant than those of concentration levels.

This study attempts to identify whether Phenethylamine (PTA), having a similar smell to fish malodor, is appropriate as a replacement of Trimethylamine (TMA). In a continuous measurement test, the odor intensity of both reagents TMA and PTA measured within 5 minutes showed between 0.4~0.7, indicating a dramatic reduction in olfactory sense, while the measurement test conducted after olfactory recovery did not represent much decrease. The result shows that both TMA and PTA have odors that stay long, but are judged to be inappropriate as a replacement as they stimulate the olfactory sense of the panel and give direct impact on the panel"s next judgement.

An analytical method for trimethylamine in ambient air was developed, using headspace gas chromatography with flame ionization detection. Trimethylamine was collected on the acid filter which was impregnated sulfuric aicd in the 47 ㎜ diameter of glass fiber filter. Trimethylamine collected on the acid filter was regenerated in the headspace vial and introduced into the GC analytical column directly. Several parameters such as sample volume, equilibrium temperature and time, and slurry method of filter were optimized to provide maximum detection response. Resolution power also compared according to liquid phase of analytical column. The detection limit of method was 0.13 ppb with 50 L sampling volume. The developed acid filter method is easy to deal with the field sampling and the method was adopted as the standard method for odor analysis in Korea.